Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,482,477	A→G	G128G (GGT→GGC)	azoR ←	FMN dependent NADH:quinone oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,482,477	0	A	G	100.0%	175.7 / NA	47	G128G (GGT→GGC)	azoR	FMN dependent NADH:quinone oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/29);  total (18/29)

CAAATTTCACATCGGTAATGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGACCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCGAGATGTTGAAGTTATACATCGGTGCCGCAATAACGATAACGTCGTGGGCTTTCAGCT  >  NC_000913/1482347‑1482613                                                                                                                                   |                                                                                                                                         cAAATTTCACATCGGTAATGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGa                                                                                                                                 <  1:176993/140‑1 (MQ=255)   aaTTTCACATCGGTAATGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGAcc                                                                                                                               <  1:524513/140‑1 (MQ=255)    aTTTCACATCGGTAATGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCg                                                                                                                              >  1:212209/1‑140 (MQ=255)        cacaTCGGTAATGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTtct                                                                                                                          <  2:251282/140‑1 (MQ=255)        cacaTCGGTAATGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTtct                                                                                                                          <  1:676759/140‑1 (MQ=255)                   tGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCgg                                                                                                               <  2:687890/140‑1 (MQ=255)                   tGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCgg                                                                                                               <  2:212209/140‑1 (MQ=255)                   tGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCgg                                                                                                               <  1:25599/140‑1 (MQ=255)                          aaaGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTaa                                                                                                        >  1:599994/1‑140 (MQ=255)                             gCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGc                                                                                                     <  2:1045510/140‑1 (MQ=255)                                gAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTg                                                                                                  <  1:34923/140‑1 (MQ=255)                                  ggAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTgcg                                                                                                <  2:571474/140‑1 (MQ=255)                                               aTACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGt                                                                                   <  1:438824/140‑1 (MQ=255)                                               aTACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGt                                                                                   >  2:452500/1‑140 (MQ=255)                                               aTACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCAGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGt                                                                                   <  2:925564/140‑1 (MQ=255)                                                     cGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAAt                                                                             >  1:406146/1‑140 (MQ=255)                                                         aCCAGGTCCGTTGGTCCATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGtt                                                                         >  2:733728/1‑140 (MQ=255)                                                              gTCCGTTGGTCCATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCa                                                                    >  1:590060/1‑140 (MQ=255)                                                                       tCCATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCg                                                           <  1:34855/140‑1 (MQ=255)                                                                        ccATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCga                                                          >  2:584437/1‑140 (MQ=255)                                                                        ccATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCga                                                          >  2:329461/1‑140 (MQ=255)                                                                        ccATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCga                                                          <  1:935875/140‑1 (MQ=255)                                                                          aTCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCgaga                                                        <  2:406146/140‑1 (MQ=255)                                                                             tttGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCGAGATGt                                                     <  2:590060/140‑1 (MQ=255)                                                                              ttGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAATTGAGTCGAGATGtt                                                    <  1:814910/140‑1 (MQ=255)                                                                              ttGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCGAGATGtt                                                    <  2:464619/140‑1 (MQ=255)                                                                               tgtgGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCGAGATGTTg                                                   <  1:187878/140‑1 (MQ=255)                                                                               tgtgGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCGAGATGTTg                                                   <  2:452183/140‑1 (MQ=255)                                                                               tgtgGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCGAGATGTTg                                                   <  2:446427/140‑1 (MQ=255)                                                                                gtgGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCGAGATGTTGa                                                  <  2:821396/140‑1 (MQ=255)                                                                                     tCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCGAGATg                                                      <  1:950972/131‑1 (MQ=255)                                                                                     tCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGGCGAGATg                                                      >  2:950972/1‑131 (MQ=255)                                                                                        tgccgcGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCGAGATGTTg                                                   <  1:79364/130‑1 (MQ=255)                                                                                        tgccgcGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCGAGATGTTg                                                   >  2:79364/2‑131 (MQ=255)                                                                                        cgccgcGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCGAGATGTTg                                                   <  2:608927/131‑1 (MQ=255)                                                                                        cgccgcGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCGAGATGTTg                                                   >  1:608927/1‑131 (MQ=255)                                                                                          ccgcGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCGAGATGTTGAAGTTATACAt                                        <  2:599994/140‑1 (MQ=255)                                                                                             cgGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCGAGATGTTGAAGTTATACATCgg                                     <  1:819433/140‑1 (MQ=255)                                                                                             cgGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCGAGATGTTGAAGTTATACATCgg                                     <  1:551275/140‑1 (MQ=255)                                                                                               gCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCGAGATGTTGAAGTTATACATCGGTg                                   >  2:904382/1‑140 (MQ=255)                                                                                                             tGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCGAGATGTTGAAGTTATACATCGGTGCCGCAataacgata                     >  1:267983/1‑140 (MQ=255)                                                                                                             tGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCGAGATGTTGAAGTTATACATCGGTGCCGCAataacgat                      >  2:1016965/1‑139 (MQ=255)                                                                                                             tGGCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCGAGATGTTGAAGTTATACATCGGTGCCGCAataacgat                      <  1:1016965/139‑1 (MQ=255)                                                                                                              ggCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCGAGATGTTGAAGTTATACATCGGTGCCGCAataacgataa                    >  2:302096/1‑140 (MQ=255)                                                                                                              ggCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCGAGATGTTGAAGTTATACATCGGTGCCGCAataacgataa                    >  2:172774/1‑140 (MQ=255)                                                                                                              ggCTTTTTTACCCGTTACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCGAGATGTTGAAGTTATACATCGGTGCCGCAataacgataa                    >  1:789338/1‑140 (MQ=255)                                                                                                                             tACCAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCGAGATGTTGAAGTTATACATCGGTGCCGCAATAACGATAACGTCGTGGGCTTTCa     >  2:378123/1‑140 (MQ=255)                                                                                                                                cAGGCCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCGAGATGTTGAAGTTATACATCGGTGCCGCAATAACGATAACGTCGTGGGCTTTCAGCt  <  2:371122/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                         CAAATTTCACATCGGTAATGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGACCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGCGGGCAACCAGGTCAAAATAGTTTTTCAACTGAGTCGAGATGTTGAAGTTATACATCGGTGCCGCAATAACGATAACGTCGTGGGCTTTCAGCT  >  NC_000913/1482347‑1482613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑