Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,523,635	A→C	N110T (AAC→ACC)	yncE →	PQQ‑like domain‑containing protein YncE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,523,635	0	A	C	100.0%	187.9 / NA	50	N110T (AAC→ACC)	yncE	PQQ‑like domain‑containing protein YncE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (32/18);  total (32/18)

CTGGATAAAGGTGGCGTGGTTTATCGTCTTGATCCGGTCACTCTGGAAGTGACGCAGGCGATCCATAACGATCTCAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAAACAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCACGGAAGAGGTGCGCCCGCTGCAACCGCGTGAGCTGGTAGCTGACGATGCCACGAACAC  >  NC_000913/1523499‑1523767                                                                                                                                         |                                                                                                                                     cTGGATAAAGGTGGCGTGGTTTATCGTCTTGATCCGGTCACTCTGGAAGTGACGCAGGCGATCCATAACGATCTCAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAg                                                                                                                                   <  2:756823/140‑1 (MQ=255)   ggATAAAGGTGGCGTGGTTTATCGTCTTGATCCGGTCACTCTGGAAGTGACGCAGGCGATCCATAACGATCTCAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAgcg                                                                                                                                 <  2:86639/140‑1 (MQ=255)   ggATAAAGGTGGCGTGGTTTATCGTCTTGATCCGGTCACTCTGGAAGTGACGCAGGCGATCCATAACGATCTCAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAgcg                                                                                                                                 <  2:650347/140‑1 (MQ=255)       aaaGGTGGCGTGGTTTATCGTCTTGATCCGGTCACTCTGGAAGTGACGCAGGCGATCCATAACGATCTCAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGt                                                                                                                             <  1:727905/140‑1 (MQ=255)              gCGTGGTTTATCGTCTTGATCCGGTCACTCTGGAAGTGACGCAGGCGATCCATAACGATCTCAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCg                                                                                                                      >  1:537607/1‑140 (MQ=255)                 tGGTTTATCGTCTTGATCCGGTCACTCTGGAAGTGACGCAGGCGATCCATAACGATCTCAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATa                                                                                                                   <  2:537607/140‑1 (MQ=255)                   gTTTATCGTCTTGATCCGGTCACTCTGGAAGTGACGCAGGCGATCCATAACGATCTCAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGa                                                                                                                 >  1:429819/1‑140 (MQ=255)                             ttGATCCGGTCACTCTGGAAGTGACGCAGGCGATCCATAACGATCTCAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGATGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACg                                                                                                       >  2:293008/1‑140 (MQ=255)                             ttGATCCGGTCACTCTGGAAGTGACGCAGGCGATCCATAACGATCTCAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACg                                                                                                       >  2:652160/1‑140 (MQ=255)                                 tCCGGTCACTCTGGAAGTGACGCAGGCGATCCATAACGATCTCAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCg                                                                                                   <  1:1018240/140‑1 (MQ=255)                                     gTCACTCTGGAAGTGACGCAGGCGATCCATAACGATCTCAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGt                                                                                               >  2:42965/1‑140 (MQ=255)                                          tctGGAAGTGACGCAGGCGATCCATAACGATCTCAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAg                                                                                          >  1:126141/1‑140 (MQ=255)                                            tGGAAGTGACGCAGGCGATCCATAACGATCTCAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGc                                                                                        >  2:972891/1‑140 (MQ=255)                                             gcaaGTGACGCAGGCGATCCATAACGATCTCAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGcc                                                                                       <  2:981872/138‑1 (MQ=255)                                              gAAGTGACGCAGGCGATCCATAACGATCTCAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCg                                                                                      >  2:817844/1‑140 (MQ=255)                                                    aCGCAGGCGATCCATAACGATCTCAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGt                                                                                >  1:39433/1‑140 (MQ=255)                                                      gCAGGCGATCCATAACGATCTCAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGc                                                                              >  1:688088/1‑140 (MQ=255)                                                         ggCGATCCATAACGATCTCAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTgg                                                                           <  2:373027/140‑1 (MQ=255)                                                                 aTAACGATCTCAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGATGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGt                                                                   <  1:293008/140‑1 (MQ=255)                                                                        tctcAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGt                                                                   <  2:320438/133‑1 (MQ=255)                                                                        tctcAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGt                                                                   >  1:320438/1‑133 (MQ=255)                                                                        tctcAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCa                                                            <  2:429819/140‑1 (MQ=255)                                                                        tctcAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCa                                                            >  1:206576/1‑140 (MQ=255)                                                                        tctcAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCa                                                            >  1:661561/1‑140 (MQ=255)                                                                        tctcAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCa                                                            >  1:360411/1‑140 (MQ=255)                                                                        tctcAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCa                                                            >  2:202157/1‑140 (MQ=255)                                                                        tctcAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCa                                                            >  2:181297/1‑140 (MQ=255)                                                                        tctcAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGCTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCa                                                            >  2:131340/1‑140 (MQ=255)                                                                          tcAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCACg                                                          >  1:644349/1‑140 (MQ=255)                                                                                  tttGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCACGGAAGAGGt                                                  >  1:385738/1‑140 (MQ=255)                                                                                        gCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCACGGAAGAGGTGCGccc                                            >  2:1004113/1‑140 (MQ=255)                                                                                                       acGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCACGGAAGAGGTGCGCCCGCTGCAACCGCGTGa                             >  2:531849/1‑140 (MQ=255)                                                                                                        cGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCACGGAAGAGGTGCGCCCGCTGCAACCGCGTGAg                            >  1:21811/1‑140 (MQ=255)                                                                                                        cGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCACGGAAGAGGTGCGCCCGCTGCAACCGCGTGAg                            >  1:382583/1‑140 (MQ=255)                                                                                                        cGACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCACGGAAGAGGTGCGCCCGCTGCAACCGCGTGAg                            >  2:436824/1‑140 (MQ=255)                                                                                                         gACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCACGGAAGAGGTGCGCCCGCTGCAGCCGCGTGAGc                           <  2:126141/140‑1 (MQ=255)                                                                                                         gACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCACGGAAGAGGTGCGCCCGCTGCAACCGCGTGAGc                           >  2:733700/1‑140 (MQ=255)                                                                                                         gACTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCACGGAAGAGGTGCGCCCGCTGCAACCGCGTGAGc                           >  1:302156/1‑140 (MQ=255)                                                                                                           cTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCACGGAAGAGGTGCGCCCGCTGCAACCGCGTGAGCTg                         >  2:372165/1‑140 (MQ=255)                                                                                                           cTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCACGGAAGAGGTGCGCCCGCTGCAACCGCGTGAGCTg                         >  2:827/1‑140 (MQ=255)                                                                                                           cTCAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCACGGAAGAGGTGCGCCCGCTGCAACCGCGTGAGCTg                         >  1:612474/1‑140 (MQ=255)                                                                                                             cAGACGTTGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCACGGAAGAGGTGCGCCCGCTGCAACCGCGTGAGCTGGt                       >  1:14217/1‑140 (MQ=255)                                                                                                                   ttGTGGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCACGGAAGAGGTGCGCCCGCTGCAACCGCGTGAGCTGGTAGCTGa                 <  1:652160/140‑1 (MQ=255)                                                                                                                     gtgGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCACGGAAGAGGTGCGCCCGCTGCAACCGCGt                               >  2:379453/1‑124 (MQ=255)                                                                                                                     gtgGTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCACGGAAGAGGTGCGCCCGCTGCAACCGCGt                               <  1:379453/124‑1 (MQ=255)                                                                                                                       ggTTTGGTAACACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCACGGAAGAGGTGCGCCCGCTGCAACCGCGTGAGCTGGTAGCTGACGAt             <  1:202157/140‑1 (MQ=255)                                                                                                                               aaCACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCACGGAAGAGGTGCGCCCGCTGCAACCGCGTGAGCTGGTAGCTGACGATGCCACGaa     <  2:433049/140‑1 (MQ=255)                                                                                                                               aaCACCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCACGGAAGAGGTGCGCCCGCTGCAACCGCGTGAGCTGGTAGCTGACGATGCCACGaa     <  1:131340/140‑1 (MQ=255)                                                                                                                                 cacCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCACGGAAGAGGTGCGCCCGCTGCAACCGCGTGAGCTGGTAGCTGACGATGCCACGAaca   <  1:817844/140‑1 (MQ=255)                                                                                                                                  acCGTAACCAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCACGGAAGAGGTGCGCCCGCTGCAACCGCGTGAGCTGGTAGCTGACGATGCCACGAacac  >  1:311589/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                     CTGGATAAAGGTGGCGTGGTTTATCGTCTTGATCCGGTCACTCTGGAAGTGACGCAGGCGATCCATAACGATCTCAAGCCGTTTGGTGCCACCATCAATAACACGACTCAGACGTTGTGGTTTGGTAACACCGTAAACAGCGCGGTCACGGCGATAGATGCCAAAACGGGCGAAGTGAAAGGCCGTCTGGTGCTGGATGATCGTAAGCGCACGGAAGAGGTGCGCCCGCTGCAACCGCGTGAGCTGGTAGCTGACGATGCCACGAACAC  >  NC_000913/1523499‑1523767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑