Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	129,270	C→T	G453G (GGC→GGT)	lpd →	lipoamide dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	129,270	0	C	T	98.5%	232.2 / ‑5.8	68	G453G (GGC→GGT)	lpd	lipoamide dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (45/22);  total (46/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCGGTGGTGCGATTGTCGGTACTAACGGCGGCGAGCTGCTGGGTGAAATCGGCCTGGCAATCGAAATGGGTTGTGATGCTGAAGACATCGCACTGACCATCCACGCGCACCCGACTCTGCACGAGTCTGTGGGCCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAA  >  NC_000913/129136‑129405                                                                                                                                       |                                                                                                                                        aTCGGTGGTGCGATTGTCGGTACTAACGGCGGCGAGCTGCTGGGTGAAATCGGCCTGGCAATCGAAATGGGTTGTGATGCTGAAGACATCGCACTGACCATCCACGCGCACCCGACTCTGCACGAGTCTGTGGGTCTggc                                                                                                                                    >  1:997813/1‑140 (MQ=255)     gtggtgCGATTGTCGGTACTAACGGCGGCGAGCTGCTGGGTGAAATCGGCCTGGCAATCGAAATGGGTTGTGATGCTGAAGACATCGCACTGACCATCCACGCGCACCCGACTCTGCACGAGTCTGTGGGTCTGGCGGCa                                                                                                                                >  1:111229/1‑140 (MQ=255)       ggtgCGATTGTCGGTACTAACGGCGGCGAGCTGCTGGGTGAAATCGGCCTGGCAATCGAAATGGGTTGTGATGCTGAAGACATCGCACTGACCATCCACGCGCACCCGACTCTGCACGAGTCTGTGGGTCTGGCGGCAGa                                                                                                                              <  1:342888/140‑1 (MQ=255)       ggtgCGATTGTCGGTACTAACGGCGGCGAGCTGCTGGGTGAAATCGGCCTGGCAATCGAAATGGGTTGTGATGCTAAAGACATCGCACTGACCATCCACGCGCACCCGACTCTGCACGAGTCTGTGGGTCTGGCGGCAGa                                                                                                                              >  1:265960/1‑140 (MQ=255)        gtgCGATTGTCGGTACTAACGGCGGCGAGCTGCTGGGTGAAATCGGCCTGGCAATCGAAATGGGTTGTGATGCTGAAGACATCGCACTGACCATCCACGCGCACCCGACTCTGCACGAGTCTGTGGGTCTGGCGGCAGaa                                                                                                                             <  1:298481/140‑1 (MQ=255)               tGTCGGTACTAACGGCGGCGAGCTGCTGGGTGAAATCGGCCTGGCAATCGAAATGGGTTGTGATGCTGAAGACATCGCACTGACCATCCACGCGCACCCGACTCTGCACGAGTCTGTGGGTCTGGCGGCAGAAGTGTTCg                                                                                                                      >  1:722548/1‑140 (MQ=255)               tGTCGGTACTAACGGCGGCGAGCTGCTGGGTGAAATCGGCCTGGCAATCGAAATGGGTTGTGATGCTGAAGACATCGCACTGACCATCCACGCGCACCCGACTCTGCACGAGTCTGTGGGTCTGGCGGCAGAAGTGTTCg                                                                                                                      <  2:394442/140‑1 (MQ=255)                gTCGGTACTAACGGCGGCGAGCTGCTGGGTGAAATCGGCCTGGCAATCGAAATGGGTTGTGATGCTGAAGACATCGCACTGACCATCCACGCGCACCCGACTCTGCACGAGTCTGTGGGTCTGGCGGCAGAAGTGTTc                                                                                                                       <  1:642818/138‑1 (MQ=255)                gTCGGTACTAACGGCGGCGAGCTGCTGGGTGAAATCGGCCTGGCAATCGAAATGGGTTGTGATGCTGAAGACATCGCACTGACCATCCACGCGCACCCGACTCTGCACGAGTCTGTGGGTCTGGCGGCAGAAGTGTTc                                                                                                                       >  2:642818/1‑138 (MQ=255)                gTCGGTACTAACGGCGGCGAGCTGCTGGGTGAAATCGGCCTGGCAATCGAAATGGGTTGTGATGCTGAAGACATCGCACTGACCATCCACGCGCACCCGACTCTGCACGAGTCTGTGGGTCTGGCGGCAGAAGTGTTCGa                                                                                                                     >  1:529012/1‑140 (MQ=255)                gTCGGTACTAACGGCGGCGAGCTGCTGGGTGAAATCGGCCTGGCAATCGAAATGGGTTGTGATGCTGAAGACATCGCACTGACCATCCACGCGCACCCGACTCTGCACGAGTCTGTGGGTCTGGCGGCAGAAGTGTTCGa                                                                                                                     >  1:474272/1‑140 (MQ=255)                gTCGGTACTAACGGCGGCGAGCTGCTGGGTGAAATCGGCCTGACAATCGAAATGGGTTGTGATGCTGAAGACATCGCACTGACCATCCACGCGCACCCGACTCTGCACGAGTCTGTGGGTCTGGCGGCAGAAGTGTTCGa                                                                                                                     >  1:370482/1‑140 (MQ=255)                 tCGGTACTAACGGCGGCGAGCTGTTGGGTGAAATCGGCCTGGCAATCGAAATGGGTTGTGATGCTGAAGACATCGCACTGACCATCCACGCGCACCCGACTCTGCACGAGTCTGTGGGTCTGGCGGCAGAAGTGTTCGaa                                                                                                                    <  2:111229/140‑1 (MQ=255)                  cGGTACTAACGGCGGCGAGCTGCTGGGTGAAATCGGCCTGGCAATCGAAATGGGTTGTGATGCTGAAGACATCGCACTGACCATCCACGCGCACCCGACTCTGCACGAGTCTGTGGGTCTGGCGGCAGAAGTGTTCGAAg                                                                                                                   >  1:837354/1‑140 (MQ=255)                     tACTAACGGCGGCGAGCTGCTGGGTGAAATCGGCCTGGCAATCGAAATGGGTTGTGATGCTGAAGACATCGCACTGACCATCCACGCGCCCCCGACTCTGCACGAGTCTGTGGGTCTGGCGGCAGAAGTGTTCGAAGGTa                                                                                                                <  2:529012/140‑1 (MQ=255)                     tACTAACGGCGGCGAGCTGCTGGGTGAAATCGGCCTGGCAATCGAAATGGGTTGTGATGCTGAAGACATCGCACTGACCATCCACGCGCACCCGACTCTGCACGAGTCTGTGGGTCTGGCGGCAGAAGTGTTCGAAGGTa                                                                                                                <  2:763011/140‑1 (MQ=255)                               ggcgAGCTGCTGGGTGAAATCGGCCTGGCAATCGAAATGGGTTGTGATGCTGAAGACATCGCACTGACCATCCACGCGCACCCGACTCTGCACGAGTCTGTGGGTCTGGCGGCAGAAGTGTTCGAAGGTAGCATTa                                                                                                          >  2:603427/1‑136 (MQ=255)                               ggcgAGCTGCTGGGTGAAATCGGCCTGGCAATCGAAATGGGTTGTGATGCTGAAGACATCGCACTGACCATCCACGCGCACCCGACTCTGCACGAGTCTGTGGGTCTGGCGGCAGAAGTGTTCGAAGGTAGCATTa                                                                                                          <  1:603427/136‑1 (MQ=255)                                      tgctgGGTGAAATCGGCCTGGCAATCGAAATGGGTTGTGATGCTAAAGACATCGCACTGACCATCCACGCGCCCCCGACTCTGCACGAGTCTGTGGGTCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCg                                                                                               <  2:265960/140‑1 (MQ=255)                                                aaTCGGCCTGGCAATCGAAATGGGTTGTGATGCTGAAGACATCGCACTGACCATCCACGCGCACCCGACTCTGCACGAGTCTGTGGGTCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAg                                                                                     >  1:384427/1‑140 (MQ=255)                                                      ccTGGCAATCGAAATGGGTTGTGATGCTGAAGACATCGCACTGACCATCCACGCGCACCCGACTCTGCACGAGTCTGTGGGTCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCgaaga                                                                               >  1:395536/1‑140 (MQ=255)                                                      ccTGGCAATCGAAATGGGTTGTGATGCTGAAGACATCGCACTGACCATCCACGCGCACCCGACTCTGCACGAGTCTGTGGGTCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCgaaga                                                                               <  2:557787/140‑1 (MQ=255)                                                         ggCAATCGAAATGGGTTGTGATGCTGAAGACATCGCACTGACCATCCACGCGCACCCGACTCTGCACGAGTCTGTGGGTCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCgaagaaga                                                                            >  2:94907/1‑140 (MQ=255)                                                         ggCAATCGAAATGGGTTGTGATGCTGAAGACATCGCACTGACCATCCACGCGCACCCGACTCTGCACGAGTCTGTGGGTCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCgaagaaga                                                                            >  1:967542/1‑140 (MQ=255)                                                                    tGGGTTGTGATGCTGAAGACATCGCACTGACCATCCACGCGCACCCGACTCTGCACGAGTCTGTGGGTCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTc                                                                 >  2:269575/1‑140 (MQ=255)                                                                       gTTGTGATGCTGAAGACATCGCACTGACCATCCACGCGCACCCGACTCTGCACGAGTCTGTGGGTCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGtt                                                              <  2:384427/140‑1 (MQ=255)                                                                        ttGTGATGCTGAAGACATCGCACTGACCATCCACGCGCACCCGACTCTGCACGAGTCTGTGGGTCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGttt                                                             >  1:834584/1‑140 (MQ=255)                                                                          gtgATGCTGAAGACATCGCACTGACCATCCACGCGCACCCGACTCTGCACGAGTCTGTGGGTCTGGCGACAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGc                                                           <  1:436392/140‑1 (MQ=255)                                                                              tGCTAAAGACATCGCACTGACCAGCCACGCGCACCCGACTCTGCACGAGTCTGTGGGTCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGa                                                       >  1:704001/1‑140 (MQ=255)                                                                                cTGAAGACATCGCACTGACCATCCACGCGCACCCGACTCTGCACGAGTCTGTGGGTCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCg                                                         <  1:616527/136‑1 (MQ=255)                                                                                cTGAAGACATCGCACTGACCATCCACGCGCACCCGACTCTGCACGAGTCTGTGGGTCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCg                                                         >  2:736751/1‑136 (MQ=255)                                                                                cTGAAGACATCGCACTGACCATCCACGCGCACCCGACTCTGCACGAGTCTGTGGGTCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCg                                                         >  2:616527/1‑136 (MQ=255)                                                                                cTGAAGACATCGCACTGACCATCCACGCGCACCCGACTCTGCACGAGTCTGTGGGTCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCg                                                         <  1:736751/136‑1 (MQ=255)                                                                                cTGAAGACATCGCACTGACCATCCACGCGCACCCGACTCTGCACGAGTCTGTGGGTCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAAc                                                     >  1:806575/1‑140 (MQ=255)                                                                                     gACATCGCACTGACCATCCACGCGCACCCGACTCTGCACGAGTCTGTGGGTCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCg                                                <  2:704001/140‑1 (MQ=255)                                                                                      aCATCGCACTGACCATCCACGCGCACCCGACTCTGCACGAGTCTGTGGGTCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCgg                                               <  1:269575/140‑1 (MQ=255)                                                                                       cATCGCACTGACCATCCACGCGCACCCGACTCTGCACGAGTCTGTGGGTCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGc                                              >  2:393628/1‑140 (MQ=255)                                                                                        aTCGCACTGACCATCCACGCGCACCCGACTCTGCACGAGTCTGTGGGTCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCa                                             >  1:468439/1‑140 (MQ=255)                                                                                        aTCGCACTGACCATCCACGCGCACCCGACTCTGCACGAGTCTGTGGGTCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCa                                             >  2:210635/1‑140 (MQ=255)                                                                                          cGCACTGACCATCCACGCGCACCCGACTCTGCACGAGTCTGTGGGTCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAAt                                           >  1:184478/1‑140 (MQ=255)                                                                                               tGACCATCCACGCGCACCCGACTCTGCACGAGTCTGTGGGTCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTaaaa                                      <  2:370482/140‑1 (MQ=255)                                                                                                  ccATCCACGCGCACCCGACTCTGCACGAGTCTGTGGGTCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAg                                   >  1:715301/1‑140 (MQ=255)                                                                                                    aTCCACGCGCACCCGACTCTGCACGAGTCTGTGGGTCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCg                                 >  1:261241/1‑140 (MQ=255)                                                                                                    aTCCACGCGCACCCGACTCTGCACGAGTCTGTGGGTCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCg                                 >  1:140277/1‑140 (MQ=255)                                                                                                      ccACGCGCACCCGACTCTGCACGAGTCTGTGGGTCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGc                               >  1:107871/1‑140 (MQ=255)                                                                                                      ccACGCGCACCCGACTCTGCACGAGTCTGTGGGTCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGc                               >  1:261568/1‑140 (MQ=255)                                                                                                       cACGCGCACCCGACTCTGCACGAGTCTGTGGGTCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCt                              >  2:535622/1‑140 (MQ=255)                                                                                                           cgcACCCGACTCTGCACGAGTCTGTGGGTCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAAcc                          >  1:633998/1‑140 (MQ=255)                                                                                                           cgcACCCGACTCTGCACGAGTCTGTGGGTCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAAcc                          >  2:939381/1‑140 (MQ=255)                                                                                                              ccccGACTCTGCACGAGTCTGTGGGTCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCAcg                       <  2:834584/139‑1 (MQ=255)                                                                                                              aCCCGACTCTGCACGAGTCTGTGGGTCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCAcg                       >  2:323525/1‑140 (MQ=255)                                                                                                                ccGACTCTGCACGAGTCTGTGGGTCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCAcgcc                     >  1:703964/1‑140 (MQ=255)                                                                                                                ccGACTCTGCACGAGTCTGTGGGTCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCAcgcc                     >  1:543348/1‑140 (MQ=255)                                                                                                                ccGACTCTGCACAAGTCTGTGGGTCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCAcgcc                     >  1:454440/1‑140 (MQ=255)                                                                                                                 cGACTCTGCACGAGTCTGTGGGTCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCAcgccg                    <  2:468439/140‑1 (MQ=255)                                                                                                                   aCTCTGCACGAGTCTGTGGGTCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCt                  <  2:107871/140‑1 (MQ=255)                                                                                                                       tGCACGAGTCTGTGGGTCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCttttt              >  1:323837/1‑140 (MQ=255)                                                                                                                       tGCACGAGTCTGTGGGTCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCttttt              >  1:923005/1‑140 (MQ=255)                                                                                                                        gCACGAGTCTGTGGGTCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCtttttt             >  1:855317/1‑140 (MQ=255)                                                                                                                         cACGAGTCTGTGGGTCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCttttttt            >  1:401451/1‑140 (MQ=255)                                                                                                                          aCGAGTCTGTGGGTCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTa           >  1:139066/1‑140 (MQ=255)                                                                                                                          aCGAGTCTGTGGGTCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTa           >  2:176041/1‑140 (MQ=255)                                                                                                                              gTCTGTGGGTCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTc       <  1:761449/140‑1 (MQ=255)                                                                                                                              gTCTGTGGGTCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTc       <  2:923005/140‑1 (MQ=255)                                                                                                                              gTCTGTGGGCCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGATGAAGAAGTAATTTTTGGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTc       >  2:927656/1‑140 (MQ=255)                                                                                                                               tCTGTGGGTCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCt      >  1:723310/1‑140 (MQ=255)                                                                                                                                 tgtgGGTCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGc    >  1:674310/1‑140 (MQ=255)                                                                                                                                 tgtgGGTCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGc    >  2:1000965/1‑140 (MQ=255)                                                                                                                                   tgGGTCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCaa  >  1:574378/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                        ATCGGTGGTGCGATTGTCGGTACTAACGGCGGCGAGCTGCTGGGTGAAATCGGCCTGGCAATCGAAATGGGTTGTGATGCTGAAGACATCGCACTGACCATCCACGCGCACCCGACTCTGCACGAGTCTGTGGGCCTGGCGGCAGAAGTGTTCGAAGGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAATTTTTCGTTTGCCGGAACATCCGGCAATTAAAAAAGCGGCTAACCACGCCGCTTTTTTTACGTCTGCAA  >  NC_000913/129136‑129405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑