Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,583,445	A→G	A81A (GCT→GCC)	ydeO ←	DNA‑binding transcriptional dual regulator YdeO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,583,445	0	A	G	100.0%	134.5 / NA	36	A81A (GCT→GCC)	ydeO	DNA‑binding transcriptional dual regulator YdeO
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/20);  total (16/20)

GTGTAATGAAACCTTTGCATGCGGCAAAAATAGACAGGCAAGAAAAAAGCAAAAGTTCTGAAAAATTATGATGATTGCGTTCTTCCGAGTTGAGATAAGCAATCATTTCATTAAGCAATCCAGTCGGTACATTTGAAGCACGTAAAAACTTCGCAGGAACAGGCACCGTCACTGTCTGGATGTTAGTGCATTGTAGATATTTTTTAATGGTATTTTCAGTAAATCCGATCTCTTTAAAATGCCGCTCATAAGGTTCAAAATGCGTACG  >  NC_000913/1583309‑1583576                                                                                                                                         |                                                                                                                                    gtgtAATGAAACCTTTGCATGCGGCAAAAATAGACAGGCAAGAAAAAAGCAAAAGTTCTGAAAAATTATGATGATTGCGTTCTTCCGAGTTGAGATAAGCAATCATTTCATTAAGCAATCCAGTCGGTACATTTGAGGCa                                                                                                                                  >  1:812320/1‑140 (MQ=255) gtgtAATGAAACCTTTGCATGCGGCAAAAATAGACAGGCAAGAAAAAAGCAAAAGTTCTGAAAAATTATGATGATTGCGTTCTTCCGAGTTGAGATAAGCAATCATTTCATTAAGCAATCCAGTCGGTACATTTGAGGCa                                                                                                                                  >  2:30222/1‑140 (MQ=255)    tAATGAAACCTTTGCATGCGGCAAAAATAGACAGGCAAGAAAAAAGCAAAAGTTCTGAAAAATTATGATGATTGCGTTCTTCCGAGTTGAGATAAGCAATCATTTCATTAAGCAATCCAGTCGGTACATTTGAGGCACGt                                                                                                                               >  2:1852/1‑140 (MQ=255)    tAATGAAACCTTTGCATGCGGCAAAAATAGACAGGCAAGAAAAAAGCAAAAGTTCTGAAAAATTATGATGATTGCGTTCTTCCGAGTTGAGATAAGCAATCATTTCATTAAGCAATCCAGTCGGTACATTTGAGGCACGt                                                                                                                               >  2:847879/1‑140 (MQ=255)     aaTGAAACCTTTGCATGCGGCAAAAATAGACAGGCAAGAAAAAAGCAAAAGTTCTGAAAAATTATGATGATTGCGTTCTTCCGAGTTGAGATAAGCAATCATTTCATTAAGCAATCCAGTCGGTACATTTGAGGCACGTa                                                                                                                              >  1:734439/1‑140 (MQ=255)            ccTTTGCATGCGGCAAAAATAGACAGGCAAGAAAAAAGCAAAAGTTCTGAAAAATTATGATGATTGCGTTCTTCCGAGTTGAGATAAGCAATCATTTCATTAAGCAATCCAGTCGGTACATTTGAGGCACGTAAAAACtt                                                                                                                       >  2:406193/1‑140 (MQ=255)              tttGCATGCGGCAAAAATAGACAGGCAAGAAAAAAGCAAAAGTTCTGAAAAATTATGATGATTGCGTTCTTCCGAGTTGAGATAAGCAATCATTTCATTAAGCAATCCAGTCGGTACATTTGAGGCACGTAAAAACTTCg                                                                                                                     <  2:903774/140‑1 (MQ=255)              tttGCATGCGGCAAAAATAGACAGGCAAGAAAAAAGCAAAAGTTCTGAAAAATTATGATGATTGCGTTCTTCCGAGTTGAGATAAGCAATCATTTCATTAAGCAATCCAGTCGGTACATTTGAGGCACGTAAAAACTTCg                                                                                                                     <  1:722922/140‑1 (MQ=255)                tGCATGCGGCAAAAATAGACAGGCAAGAAAAAAGCAAAAGTTCTGAAAAATTATGATGATTGCGTTCTTCCGAGTTGAGATAAGCAATCATTTCATTAAGCAATCCAGTCGGTACATTTGAGGCACGTAAAAACTTCGCa                                                                                                                   <  1:465203/140‑1 (MQ=255)                 gCATGCGGCAAAAATAGACAGGCAAGAAAAAAGCAAAAGTTCTGAAAAATTATGATGATTGCGTTCTTCCGAGTTGAGATAAGCAATCATTTCATTAAGCAATCCAGTCGGTACATTTGAGGCACGTAAAAACTTCGCAg                                                                                                                  <  1:260129/140‑1 (MQ=255)                    tGCGGCAAAAATAGACAGGCAAGAAAAAAGCAAAAGTTCTGAAAAATTATGATGATTGCGTTCTTCCGAGTTGAGATAAGCAATCATTTCATTAAGCAATCCAGTCGGTACATTTGAGGCACGTAAAAACTTCGCAGGaa                                                                                                               <  1:1852/140‑1 (MQ=255)                        gCAAAAATAGACAGGCAAGAAAAAAGCAAAAGTTCTGAAAAATTATGATGATTGCGTTCTTCCGAGTTGAGATAAGCAATCATTTCATTAAGCAATCCAGTCGGTACATTTGAGGCACGTAAAAACTTCGCAGGAACAgg                                                                                                           <  2:286877/140‑1 (MQ=255)                           aaaaTAGACAGGCAAGAAAAAAGCAAAAGTTCTGAAAAATTATGATGATTGCGTTCTTCCGAGTTGAGATAAGCAATCATTTCATTAAGCAATCCAGTCGGTACATTTGAGGCACGTAAAAACTTCGCAGGAACAGGCAc                                                                                                        >  1:744581/1‑140 (MQ=255)                                aGACAGGCAAGAAAAAAGCAAAAGTTCTGAAAAATTATGATGATTGCGTTCTTCCGAGTTGAGATAAGCAATCATTTCATTAAGCAATCCAGTCGGTACATTTGAGGCACGTAAAAACTTCGCAGGAACAGGCACCGTCa                                                                                                   <  2:120756/140‑1 (MQ=255)                                aGACAGGCAAGAAAAAAGCAAAAGTTCTGAAAAATTATGATGATTGCGTTCTTCCGAGTTGAGATAAGCAATCATTTCATTAAGCAATCCAGTCGGTACATTTGAGGCACGTAAAAACTTCGCAGGAACAGGCACCGTCa                                                                                                   <  2:505714/140‑1 (MQ=255)                                aGACAGGCAAGAAAAAAGCAAAAGTTCTGAAAAATTATGATGATTGCGTTCTTCCGAGTTGAGATAAGCAATCATTTCATTAAGCAATCCAGTCGGTACATTTGAGGCACGTAAAAACTTCGCAGGAACAGGCACCGTCa                                                                                                   <  2:538752/140‑1 (MQ=255)                                aGACAGGCAAGAAAAAAGCAAAAGTTCTGAAAAATTATGATGATTGCGTTCTTCCGAGTTGAGATAAGCAATCATTTCATTAAGCAATCCAGTCGGTACATTTGAGGCACGTAAAAACTTCGCAGGAACAGGCACCGTCa                                                                                                   <  2:812320/140‑1 (MQ=255)                                    aGGCAAGAAAAAAGCAAAAGTTCTGAAAAATTATGATGATTGCGTTCTTCCGAGTTGAGATAAGCAATCATTTCATTAAGCAATCCAGTCGGTACATTTGAGGCACGTAAAAACTTCGCAGGAACAGGCACCGTCACTGt                                                                                               <  2:744581/140‑1 (MQ=255)                                      gCAAGAAAAAAGCAAAAGTTCTGAAAAATTATGATGATTGCGTTCTTCCGAGTTGAGATAAGCAATCATTTCATTAAGCAATCCAGTCGGTACATTTGAGGCACGTAAAAACTTCGCAGGAACAGGCACCGTCACTGTCt                                                                                             >  1:477321/1‑140 (MQ=255)                                       cAAGAAAAAAGCAAAAGTTCTGAAAAATTATGATGATTGCGTTCTTCCGAGTTGAGATAAGCAATCATTTCATTAAGCAATCCAGTCGGTACATTTGAGGCACGTAAAAACTTCGCAGGAACAGGCACCGTCACTGTCTg                                                                                            <  1:30222/140‑1 (MQ=255)                                              aaaGCAAAAGTTCTGAAAAATTATGATGATTGCGTTCTTCCGAGTTGAGATAAGCAATCATTTCATTAAGCAATCCAGTCGGTACATTTGAGGCACGTAAAAACTTCGCAGGAACAGGCACCGTCACTGTCTGGATGTTa                                                                                     <  1:31030/140‑1 (MQ=255)                                                aGCAAAAGTTCTGAAAAATTATGATGATTGCGTTCTTCCGAGTTGAGATAAGCAATCATTTCATTAAGCAATCCAGTCGGTACATTTGAGGCACGTAAAAACTTCGCAGGAACAGGCACCGTCACTGTCTGGa                                                                                          <  1:26359/133‑1 (MQ=255)                                                aGCAAAAGTTCTGAAAAATTATGATGATTGCGTTCTTCCGAGTTGAGATAAGCAATCATTTCATTAAGCAATCCAGTCGGTACATTTGAGGCACGTAAAAACTTCGCAGGAACAGGCACCGTCACTGTCTGGa                                                                                          >  2:26359/1‑133 (MQ=255)                                                aGCAAAAGTTCTGAAAAATTATGATGATTGCGTTCTTCCGAGTTGAGATAAGCAATCATTTCATTAAGCAATCCAGTCGGTACATTTGAGGCACGTAAAAACTTCGCAGGAACAGGCACCGTCACTGTCTGGATGTTAGt                                                                                   >  1:77713/1‑140 (MQ=255)                                                aGCAAAAGTTCTGAAAAATTATGATGATTGCGTTCTTCCGAGTTGAGATAAGCAATCATTTCATTAAGCAATCCAGTCGGTACATTTGAGGCACGTAAAAACTTCGCAGGAACAGGCACCGTCACTGTCTGGATGTTAGt                                                                                   <  2:77713/140‑1 (MQ=255)                                                aGCAAAAGTTCTGAAAAATTATGATGATTGCGTTCTCCCGAGTTGAGATAAGCAATCATTTCATTAAGCAATCCAGTCGGTACATTTGAGGCACGTAAAAACTTCGCAGGAACAGGCACCGTCACTGTCTGGATGt                                                                                       >  1:125020/1‑136 (MQ=255)                                                aGCAAAAGTTCTGAAAAATTATGATGATTGCGTTCTCCCGAGTTGAGATAAGCAATCATTTCATTAAGCAATCCAGTCGGTACATTTGAGGCACGTAAAAACTTCGCAGGAACAGGCACCGTCACTGTCTGGATGt                                                                                       <  2:125020/136‑1 (MQ=255)                                                                  ttATGATGATTGCGTTCTTCCGAGTTGAGATAAGCAATCATTTCATTAAGCAATCCAGTCGGTACATTTGAGGCACGTAAAAACTTCGCAGGAACAGGCACCGTCACTGTCTGGATGTTAGTGCATTGTAGATAtttttt                                                                 >  1:434049/1‑140 (MQ=255)                                                                   tATGATGATTGCGTTCTTCCGAGTTGAGATAAGCAATCATTTCATTAAGCAATCCAGTCGGTACATTTGAGGCACGTAAAAACTTCGCAGGAACAGGCACCGTCACTGTCTGGATGTTAGTGCATTGTAGATATTTTTTa                                                                <  2:734439/140‑1 (MQ=255)                                                                      gatgatTGCGTTCTTCCGAGTTGAGATAAGCAATCATTTCATTAAGCAATCCAGTCGGTACATTTGAGGCACGTAAAAACTTCGCAGGAACAGGCACCGTCACTGTCTGGATGTTAGTGCATTGTAGATATTTTTTAATg                                                             <  1:640153/140‑1 (MQ=255)                                                                                   ttcCGAGTTGAGATAAGCAATCATTTCATTAAGCAATCCAGTCGGTACATTTGAGGCACGTAAAAACTTCGCAGGAACAGGCACCGTCACTGTCTGGATGTTAGTGCATTGTAGATATTTTTTAATGGTATTTTCAGTaa                                                >  2:700851/1‑140 (MQ=255)                                                                                         gTTGAGATAAGCAATCATTTCATTAAGCAATCCAGTCGGTACATTTGAGGCACGTAAAAACTTCGCAGGAACAGGCACCGTCACTGTCTGGATGTTAGTGCATTGTAGATATTTTTTAATGGTATTTTCAGTAAATCCGa                                          <  1:847879/140‑1 (MQ=255)                                                                                            gagaTAAGCAATCATTTCATTAAGCAATCCAGTCGGTACATTTGAGGCACGTAAAAACTTCGCAGGAACAGGCACCGTCACTGTCTGGATGTTAGTGCATTGTAGATATTTTTTAATGGTATTTTCAGTAAATCCGAtct                                       <  1:497906/140‑1 (MQ=255)                                                                                             agaTAAGCAATCATTTCATTAAGCAATCCAGTCGGTACATTTGAGGCACGTAAAAACTTCGCAGGAACAGGCACCGTCACTGTCTGGATGTTAGTGCATTGTAGATATTTTTTAATGGTATTTTCAGTAAATCCGAtctc                                      >  1:359671/1‑140 (MQ=255)                                                                                                                                 aCATTTGAGGCACGTAAAAACTTCGCAGGAACAGGCACCGTCACTGTCTGGATGTTAGTGCATTGTAGATATTTTTTAATGGTATTTTCAGTAAATCCGATCTCTTTAAAATGCCGCTCATAAGGTTCAAAATGCGTACg  >  2:867124/1‑140 (MQ=255)                                                                                                                                 aCATTTGAGGCACGTAAAAACTTCGCAGGAACAGGCACCGTCACTGTCTGGATGTTAGTGCATTGTAGATATTTTTTAATGGTATTTTCAGTAAATCCGATCTCTTTAAAATGCCGCTCATAAGGTTCAAAATGCGTACg  >  2:987610/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                    GTGTAATGAAACCTTTGCATGCGGCAAAAATAGACAGGCAAGAAAAAAGCAAAAGTTCTGAAAAATTATGATGATTGCGTTCTTCCGAGTTGAGATAAGCAATCATTTCATTAAGCAATCCAGTCGGTACATTTGAAGCACGTAAAAACTTCGCAGGAACAGGCACCGTCACTGTCTGGATGTTAGTGCATTGTAGATATTTTTTAATGGTATTTTCAGTAAATCCGATCTCTTTAAAATGCCGCTCATAAGGTTCAAAATGCGTACG  >  NC_000913/1583309‑1583576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑