Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,603,730	A→G	T238A (ACC→GCC)	lsrC →	Autoinducer‑2 ABC transporter membrane subunit LsrC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,603,730	0	A	G	100.0%	66.2 / NA	19	T238A (ACC→GCC)	lsrC	Autoinducer‑2 ABC transporter membrane subunit LsrC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (3/16);  total (3/16)

ACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTT  >  NC_000913/1603597‑1603863                                                                                                                                      |                                                                                                                                      aCAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCCGATTGGGTTTATTCCCAACCAAACCGGGGCCgggg                                                                                                                                 >  2:334380/1‑139 (MQ=255)     ggCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTTCTTCGCCGATTTGGTTTTTCCCCCACCAGACCGGGGCCGGGCTggg                                                                                                                             >  1:92577/1‑139 (MQ=255)             tCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTGCCGGGCTGGAGATGAAAg                                                                                                                     <  2:621644/140‑1 (MQ=255)             tCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTGCCGGGCTGGAGATGAAAg                                                                                                                     <  2:311213/140‑1 (MQ=255)                    ggCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGGATTGTGTTTGCTTCGCAGATTGGTTTTTTCCCCCACCAGACCCGGGCCGGGGTGGGGATGAAAGCAAATGc                                                                                                              >  2:597403/1‑140 (MQ=255)                         tCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTGCCGGGCTGGAGATGAAAGCAATTGCAGCCt                                                                                                         <  2:414166/140‑1 (MQ=255)                                 aaGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGTTTCGCCGATTGGTTTTTTCCCCCACCAGACCGGGGCCGGGGTGGGGATGAAAGCAATTGCAGCCTGCGTGCTg                                                                                                 >  2:644096/1‑140 (MQ=255)                                  aGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTGCCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTgg                                                                                                <  2:708700/140‑1 (MQ=255)                                       ttCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGGATTGTGTTTTCTTCGCAGATTGGTTTTTTTCCCCACCAGACCGGTGCCGGGGTGGAGGTGGAAGGAATTGTAGCCCGCGGGGTGggcggg                                                                                           >  2:205708/1‑139 (MQ=255)                                       ttCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTGCCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGggcggc                                                                                           <  1:205708/140‑1 (MQ=255)                                            aTTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTGCCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAg                                                                                      <  2:8028/140‑1 (MQ=255)                                             ttGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGGATTGTGTTTGCTTCGCAGGATGGGTTTATTCCCCACCACACCCGGGCCGGGGTGGAGATGGAAGCAAATGCAAGCTGCGTGCCGGGCGGCCTTAgg                                                                                     >  2:633201/1‑139 (MQ=255)                                                tgGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGGGCCGGGCTGGGGATGAAAGCAATTTCAGCCTGCGTGCTGGGCGGCATTAGtttt                                                                                  >  2:745650/1‑139 (MQ=255)                                                               aCGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTGCCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGt                                                                   <  1:934120/140‑1 (MQ=255)                                                               aCGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTGCCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGt                                                                   <  1:558142/140‑1 (MQ=255)                                                                        tGGCGGCACTGGCGGGAATTGTGTTTTCTTCGCAGATTTGTTTTTTCCCCCACCAGACCGGGGCCGGGGTGGGGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGGATTTGTTTGCTCGGTGGGTCCGGGGCGGTCAtt                                                          >  2:551490/1‑140 (MQ=255)                                                                         ggcggcACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTGCCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTg                                                         <  2:450983/140‑1 (MQ=255)                                                                                   gCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTGCCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACt                                               <  2:516488/140‑1 (MQ=255)                                                                                   gCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTGCCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACt                                               <  1:334380/140‑1 (MQ=255)                                                                                      ggAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTGCCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCgg                                            <  1:551490/140‑1 (MQ=255)                                                                                              gtTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTGCCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGt                                    >  1:1010488/1‑140 (MQ=255)                                                                                                         aGATTGGTTTTATCCCCAACCAGACCGGTGCCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAg                         <  1:767653/140‑1 (MQ=255)                                                                                                              ggTTTTATCCCCAACCAGACCGGTGCCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGa                    <  1:597403/140‑1 (MQ=255)                                                                                                                ttttATCCCCAACCAGACCGGTGCCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGTTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATa                  >  1:679677/1‑140 (MQ=255)                                                                                                                      ccccAACCAGACCGGTGCCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTAc            <  2:92577/140‑1 (MQ=255)                                                                                                                           aCCAGACCGGTGCCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTg       <  1:603328/140‑1 (MQ=255)                                                                                                                                aCCGGTGCCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGtt  >  2:85330/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                      ACAGGGCGCTCGTCAACTGGGCGTTCGTACTGAAGCCATTCGCATTGTGGCATTTTCGTTGAACGGCTGCATGGCGGCACTGGCGGGAATTGTGTTTGCTTCGCAGATTGGTTTTATCCCCAACCAGACCGGTACCGGGCTGGAGATGAAAGCAATTGCAGCCTGCGTGCTGGGCGGCATTAGTTTGCTCGGTGGTTCCGGTGCGATCATTGGTGCGGTACTCGGCGCATGGTTCCTGACGCAGATCGATAGCGTACTGGTGCTGTT  >  NC_000913/1603597‑1603863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑