Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,627,973	T→C	F153L (TTT→CTT)	ydfG →	3‑hydroxy acid dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,627,973	0	T	C	100.0%	205.8 / NA	56	F153L (TTT→CTT)	ydfG	3‑hydroxy acid dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (38/18);  total (38/18)

AGGCCTGGTATATATGACGCGCGCCGTCTTACCGGGTATGGTTGAACGTAATCATGGTCATATTATTAACATTGGCTCAACGGCAGGTAGCTGGCCGTATGCCGGTGGTAACGTTTACGGTGCGACGAAAGCGTTTGTTCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTACGGCGGTGCGCGTCACCGACATCGAACCGGGTCTGGTGGGTGGTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTA  >  NC_000913/1627840‑1628102                                                                                                                                      |                                                                                                                                  aGGCCTGGTATATATGACGCGCGCCGTCTTACCGGGTATGGTTGAACGTAATCATGGTCATATTATTAACATTGGCTCAACGGCAGGTAGCTGGCCGTATGCCGGTGGTAACGTTTACGGTGCGACGAAAGCGCTTGTTc                                                                                                                             >  1:598201/1‑140 (MQ=255)      tGGTATATATGACGCGCGCCGTCTTACCGGGTATGGTTGAACGTAATCATGGTCATATTATTAACATTGGCTCAACGGCAGGTAGCTGGCCGTATGCCGGTGGTAACGTTTACGGTGCGACGAAAGCGCTTGTTcacagt                                                                                                                        >  1:923980/1‑135 (MQ=255)        gTATATATGACGCGCGCCGTCTTACCGGGTATGGTTGAACGTAATCATGGTCATATTATTAACATTGGCTCAACGGCAGGTAGCTGGCCGTATGCCGGTGGTAACGTTTACGGTGCGACGAAAGCGCTTGTTCGTCAGtt                                                                                                                      <  1:331870/140‑1 (MQ=255)                gACGCGCGCCGTCTTACCGGGTATGGTTGAACGTAATCATGGTCATATTATTAACATTGGCTCAACGGCAGGTAGCTGGCCGTATGCCGGTGGTAACGTTTACGGTGCGACGAAAGCGCTTGTTCGTCAGTTTAGCCTGa                                                                                                              <  1:1040122/140‑1 (MQ=255)                  cgcgcgCCGTCTTACCGGGTATGGTTGAACGTAATCATGGTCATATTATTAACATTGGCTCAACGGCAGGTAGCTTGCCGTATGCCGGTGGCAACGTTTACGGTGCGACGAAAGCGCTTGTTCGTCAGTTTAGCCTGAAt                                                                                                            >  2:1049118/1‑140 (MQ=255)                  cgcgcgCCGTCTTACCGGGTATGGTTGAACGTAATCATGGTCATATTATTAACATTGGCTCAACGGCAGGTAGCTGGCCGTATGCCGGTGGTAACGTTTTCGGTGCGACGAAAGCGCTTGTTCGTCAGTTTAGCCTGaaa                                                                                                            >  2:922747/1‑139 (MQ=255)                  cgcgcgCCGTCTTACCGGGTATGGTTGAACGTAATCATGGTCATATTATTAACATTGGCTCAACGGCAGGTAGCTGGCCGTATGCCGGTGGTAACGTTTACGGTGCGACGAAAGCGCTTGTTCGTCAGTTTAGCCTGAAt                                                                                                            >  2:480682/1‑140 (MQ=255)                  cgcgcgCCGTCTTACCGGGTATGGTTGAACGTAATCATGGTCATATTATTAACATTGGCTCAACGGCAGGTAGCTGGCCGTATGCCGGTGGTAACGTTTACGGTGCGACGAAAGCGCTTGTTCGTCAGTTTAGCCTGAAt                                                                                                            <  1:203271/140‑1 (MQ=255)                  cgcgcgCCGTCTTACCGGGTATGGTTGAACGTAATCATGGTCATATTATTAACATTGGCTCAACGGCAGGTAGCTGGCCGTATGCCGGTGGTAACGTTTACGGTGCGACGAAAGCGCTTGTTCGTCAGTTTAGCCTGAAt                                                                                                            >  1:293062/1‑140 (MQ=255)                  cgcgcgCCGTCTTACCGGGTATGGTTGAACGTAATCATGGTCATATTATTAACATTGGCTCAACGGCAGGTAGCTGGCCGTATGCCGGTGGTAACGTTTACGGTGCGACGAAAGCGCTTGTTCGTCAGTTTAGCCTGAAt                                                                                                            >  1:335345/1‑140 (MQ=255)                  cgcgcgCCGTCTTACCGGGTATGGTTGAACGTAATCATGGTCATATTATTAACATTGGCTCAACGGCAGGTAGCTGGCCGTATGCCGGTGGTAACGTTTACGGTGCGACGAAAGCGCTTGTTCGTCAGTTTAGCCTGAAt                                                                                                            >  1:644921/1‑140 (MQ=255)                  cgcgcgCCGTCTTACCGGGTATGGTTGAACGTAATCATGGTCATATTATTAACATTGGCTCAACGGCAGGTAGCTGGCCGTATGCCGGTGGTAACGTTTACGGTGCGACGAAAGCGCTTGTTCGACAGTTTAGCCTGAAt                                                                                                            >  2:82544/1‑140 (MQ=255)                   gcgcgcCGTCTTACCGGGTATGGTTGAACGTAATCATGGTCATATTATTAACATTGGCTCAACGGCAGGTAGCTGGCCGTATGCCGGTGGTAACGTTTACGGTGCGACGAAAGCGCTTGTTCGTCAGTTTAGCCTGAATc                                                                                                           >  2:28346/1‑140 (MQ=255)                   gcgcgcCGTCTTACCGGGTATGGTTGAACGTAATCATGGTCATATTATTAACATTGGCTCAACGGCAGGTAGCTGGCCGTATGCCGGTGGTAACGTTTACGGTGCGACGAAAGCGCTTGTTCGTCAGTTTAGCCTGAATc                                                                                                           >  2:623411/1‑140 (MQ=255)                    cgcgcCGTCTTACCGGGTATGGTTGAACGTAATCATGGTCATATTATTAACATTGGCTCAACGGCAGGTAGCTGGCCGTATGCCGGTGGTAACGTTTACGGTGCGACGAAAGCGCTTGTTCGTCAGTTTAGCCTGAATCt                                                                                                          >  2:156624/1‑140 (MQ=255)                     gcgcCGTCTTACCGGGTATGGTTGAACGTAATCATGGTCATATTATTAACATTGGCTCAACGGCAGGTAGCTGGCCGTATGCCGGTGGTAACGTTTACGGTGCGACGAAAGCGCTTGTTCGTCAGTTTAGCCTGAATCTg                                                                                                         >  2:496032/1‑140 (MQ=255)                      cgcCGTCTTACCGGGTATGGTTGAACGTAATCATGGTCATATTATTAACATTGGCTCAACGGCAGGTAGCTGGCCGTATGCCGGTGGTAACGTTTACGGTGCGACGAAAGCGCTTGTTCGTCAGTTTAGCCTGAATCTGc                                                                                                        >  1:390104/1‑140 (MQ=255)                       gcCGTCTTACCGGGTATGGTTGAACGTAATCATGGTCATATTATTAACATTGGCTCAACGGCAGGTAGCTGGCCGTATGCCGGTGGTAACGTTTACGGTGCGACGAAAGCGCTTGTTCGTCAGTTTAGCCTGAATCTGCg                                                                                                       <  2:390104/140‑1 (MQ=255)                            cTTACCGGGTATGGTTGAACGTAATCATGGTCATATTATTAACATTGGCTCAACGGCAGGTAGCTGGCCGTATGCCGGTGGTAACGTTTACGGTGCGACGAAAGCGCTTGTTCGTCAGTTTAGCCTGAATCTGCGTACgg                                                                                                  <  1:163050/140‑1 (MQ=255)                                     tATGGTTGAACGTAATCATGGTCATATTATTAACATTGGCTCAACGGCAGGTAGCTGGCCGTATGCCGGTGGTAACGTTTACGGTGCGACGAAAGTGCTTGTTCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATg                                                                                         >  2:2745/1‑140 (MQ=255)                                       tGGTTGAACGTAATCATGGTCATATTATTAACATTGGCTCAACGGCAGGTAGCTGGCCGTATGCCGGTGGTAACGTTTACGGTGCGACGAAAGCGCTTGTTCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGt                                                                                       >  1:247030/1‑140 (MQ=255)                                        ggTTGAACGTAATCATGGTCATATTATTAACATTGGCTCAACGGCAGGTAGATGGCCGTATGCCGGTGGTAACGTTTACGGTGCGACGAAAGCGCTTGTTCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTa                                                                                      >  2:947451/1‑140 (MQ=255)                                         gTTGAACGTAATCATGGTCATATTATTAACATTGGCTCAACGGCAGGTAGCTGGCCGTATGCCGGTGGTAACGTTTACGGTGCGACGAAAGCGCTTGTTCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTAc                                                                                     >  1:498879/1‑140 (MQ=255)                                         gTTGAACGTAATCATGGTCATATTATTAACATTGGCTCAACGGCAGGTAGCTGGCCGTATGCCGGTGGTAACGTTTACGGTGCGACGAAAGCGCTTGTTCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTAc                                                                                     >  1:664473/1‑140 (MQ=255)                                           tGAACGTAATCATGGTCATATTATTAACATTGGCTCAACGGCAGGTAGCTGGCCGTATGCCGGTGGTAACGTTTACGGTGCGACGAAAGCGCTTGTTCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTAcgg                                                                                   <  2:664473/140‑1 (MQ=255)                                             aaCGTAATCATGGTCATATTATTAACATTGGCTCAACGGCAGGTAGCTGGCCGTATGCCGGTGGTAACGTTTACGGTGCGACGAAAGCGCTTGTTCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCa                                                                                           <  1:961856/130‑1 (MQ=255)                                             aaCGTAATCATGGTCATATTATTAACATTGGCTCAACGGCAGGTAGCTGGCCGTATGCCGGTGGTAACGTTTACGGTGCGACGAAAGCGCTTGTTCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCa                                                                                           >  2:961856/1‑130 (MQ=255)                                             aaCGTAATCATGGTCATATTATTAACATTGGCTCAACGGCAGGTAGCTGGCCGTATGCCGGTGGTAACGTTTACGGTGCGACGAAAGCGCTTGTTCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTAcggcg                                                                                 <  2:866905/140‑1 (MQ=255)                                             aaCGTAATCATGGTCATATTATTAACATTGGCTCAACGGCAGGTAGCTGGCCGTATGCCGGTGGTAACGTTTACGGTGCGACGAAAGCGCTTGTTCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTAcggcg                                                                                 >  2:233061/1‑140 (MQ=255)                                                       tGGTCATATTATTAACATTGGCTCAACGGCAGGTAGCTGGCCGTATGCCGGTGGTAACGTTTACGGTGCGACGAAAGCGCTTGTTCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTACGGCGGGGCGCGTcc                                                                       >  2:836194/1‑139 (MQ=255)                                                        ggTCATATTATTAACATTGGCTCAACGGCAGGTAGCTGGCCGTATGCCGGTGGTAACGTTTACGGTGCGACGAAAGCGCTTGTTCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTACGGCGGGGggcggcgc                                                                      >  2:529066/1‑136 (MQ=255)                                                           cATATTATTAACATTGGCTCAACGGCAGGTAGCTGGCCGTATGCCGGTGGTAACGTTTACGGTGCGAGGAAAGCGCTTGTTCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTACGGCGGGGCGCGTCAcccc                                                                   >  2:963524/1‑138 (MQ=255)                                                              attattAACATTGGCTCAACGGCAGGTAGCTGGCCGTATGCCGGTGGTAACGTTTACGGTGCGACGAAAGTGCTTGTTCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTACGGCGGTGCGCGTCACCGACAt                                                                <  1:2745/140‑1 (MQ=255)                                                               ttattaACATTGGCTCAACGGCAGGTAGCTGGCCGTATGCCGGTGGTAACGTTTACGGTGCGACGAAAGCGCTTGTTCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTACGGCGGTGCGCGTCACCGACa                                                                 >  2:827175/1‑138 (MQ=255)                                                               ttattaACATTGGCTCAACGGCAGGTAGCTGGCCGTATGCCGGTGGTAACGTTTACGGTGCGACGAAAGCGCTTGTTCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTACGGCGGTGCGCGTCACCGACa                                                                 <  1:827175/138‑1 (MQ=255)                                                                    aaCATTGGCTCAACGGCAGGTAGCTGGCCGTATGCCGGTGGTAACGTTTACGGTGCGACGAAAGCGCTTGTTCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTACGGCGGTGCGCGTCACCGACATCGAAcc                                                          <  1:480682/140‑1 (MQ=255)                                                                        ttGGCTCAACGGCAGGTAGCTGGCCGTATGCCGGTGGTAACGTTTATGGTGCGACGAAAGCGCTTGTTCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTACGGCGGTGCGCGTCACCGACATCGAACCGGGt                                                      >  1:537032/1‑140 (MQ=255)                                                                        ttGGCTCAACGGCAGGTAGCTGGCCGTATGCCGGTGGTAACGTTTATGGTGCGACGAAAGCGCTTGTTCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTACGGCGGTGCGCGTCACCGACATCGAACCGGGt                                                      <  2:537032/140‑1 (MQ=255)                                                                        ttGGCTCAACGGCAGGTAGCTGGCCGTATGCCGGTGGTAACGTTTACGGTGCGACGAAAGCGCTTGTTCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTACGGCGGTGCGCGTCACCGACATCGAACCGGGt                                                      <  2:498879/140‑1 (MQ=255)                                                                         tGGCTCAACGGCAGGTAGCTGGCCGTATGCCGGTGGTAACGTTTACGGTGCGACGAAAGCGCTTGTTCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTACGGCGGTGCGCGTCACCGACATCGAACCGGGTc                                                     >  1:369477/1‑140 (MQ=255)                                                                          ggCTCAACGGCAGGTAGCTGGCCGTATGCCGGTGGTAACGTTTACGGTGCGACGAAAGCGCTTGTTCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTACGGCGGTGGGCGGCACCGACATCCAACCcgggtt                                                    >  2:346571/1‑137 (MQ=255)                                                                          ggCTCAACGGCAGGTAGCTGGCCGTATGCCGGTGGTAACGTTTACGGTGCGACGAAAGCGCTTGTTCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTACGGCGGTGCGCGTCACCGACATCGAACCGGGTCt                                                    >  1:416874/1‑140 (MQ=255)                                                                          ggCTCAACGGCAGGTAGCTGGCCGTATGCCGGTGGTAACGTTTACGGTGCGACGAAAGCGCTTGTTCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTACGGCGGTGCGCATCACCGACATCCAACCGGGGCt                                                    >  2:577429/1‑140 (MQ=255)                                                                           gCTCAACGGCAGGTAGCTGGCCGTATGCCGGTGGTAACGTTTACGGTGCGACGAAAGCGCTTGTTCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTACGGCGGGGCGCGTCCCCCACATCGAACCGGGGCtg                                                   >  2:23107/1‑140 (MQ=255)                                                                            cTCAACGGCAGGTAGCTGGCCGTATGCCGGTGGTAACGTTTACGGTGCGACGAAAGCGCTTGTTCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTACGGCGGTGCGCGTCACCGACATCGAACCGGGGCtgg                                                  >  1:864109/1‑140 (MQ=255)                                                                                     aGGTAGCTGGCCGTATGCCGGTGGTAACGTTTACGGTGCGACGAAAGCGCTTGTTCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTACGGCGGTGCGCGTCACCGACATCGAACCGGGTCTGGTGGGTGGTa                                         <  1:963524/140‑1 (MQ=255)                                                                                         aGCTGGCCGTATGCCGGTGGTAACGTTTACGGTGCGACGAAAGCGCTTGTTCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTACGGCGGTGCGCGTCACCGACATCGAACCGGGTCTGGTGGGTGGTACCGa                                     <  2:598201/140‑1 (MQ=255)                                                                                           cTGGCCGTATGCCGGTGGTAACGTTTACGGTGCGACGAAAGCGCTTGTTCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTACGGCGGTGCGCGTCACCCACATCGGACCGGGGCTGGTGGGGGGGACCCAGt                                   >  1:962542/1‑140 (MQ=255)                                                                                              gCCGTATGCCGGTGGTAACGTTTACGGTGCGACGAAAGCGCTTGTTCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTACGGCGGTGCGCGGCACCGACATCGAACCGGGGCTGGGGGGGGGGACCGAGgttt                                >  2:167145/1‑140 (MQ=255)                                                                                                  tATGCCGGTGGTAACGTTTACGGTGCGACGAAAGCGCTTGTTCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTACGGCGGTGCGCGTCACCGACATCGAACCGGGTCTGGTGGGTGGTACCGAGTTTTCCaa                            >  2:209762/1‑140 (MQ=255)                                                                                                  tATGCCGGTGGTAACGTTTACGGTGCGACGAAAGCGCTTGTTCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTACGGCGGTGCGCGTCACCGACATCGAACCGGGGCTGGGGGGGGGGACCGAGGTTTCCaa                            >  2:353034/1‑140 (MQ=255)                                                                                                      ccGGTGGTAACGTTTACGGTGCGACGAAAGCGCTTGTTCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTACGGCGGTGCGCGTCACCGACATCGAACCGGGTCTGGTGGGTGGTACCGAGTTTTCCAATGTc                        <  2:247030/140‑1 (MQ=255)                                                                                                      ccGGTGGTAACGTTTACGGTGCGACGAAAGCGCTTGTTCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTACGGCGGTGCGCGTCACCGACATCGAACCGGGTCTGGTGGGTGGTACCGAGTTTTCCAATGTc                        <  1:947451/140‑1 (MQ=255)                                                                                                        ggtggtAACGTTTACGGTGCGACGAAAGCGCTTGTTCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTACGGCGGTGCGCGTCACCGACATCGAACCGGGTCTGGTGGGTGGGACCGAGGTTTTCAAAGTCCg                      >  2:468948/1‑140 (MQ=255)                                                                                                                cGTTTACGGTGCGACGAAAGCGCTTGTTCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTACGGCGGTGCGCGTCACCGACATCGAACCGGGTCTGGTGGGTGGTACCGAGTTTTCCAATGTCCGCTTTAAAg              <  1:55361/140‑1 (MQ=255)                                                                                                                    tACGGTGCGACGAAAGCGCTTGTTCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTACGGCGGTGCGCGGCACCGACATCGAACCGGGGCTGGGGGGTGGTACCGAGGTTTCCAATGTCCGCTTTAAAGGCGa          >  2:549467/1‑140 (MQ=255)                                                                                                                     aCGGTGCGACGAAAGCGCTTGTTCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTACGGCGGTGCGCGTCACCGACATCGAACCGGGTCTGGTGGGTGGTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGAt         >  1:341745/1‑140 (MQ=255)                                                                                                                            gacgaAAGCGCTTGTTCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTACGGCGGTGCGCGTCACCGACATCGAACCGGGTCTGGTGGGTGGTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTa  >  1:560876/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                  AGGCCTGGTATATATGACGCGCGCCGTCTTACCGGGTATGGTTGAACGTAATCATGGTCATATTATTAACATTGGCTCAACGGCAGGTAGCTGGCCGTATGCCGGTGGTAACGTTTACGGTGCGACGAAAGCGTTTGTTCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTACGGCGGTGCGCGTCACCGACATCGAACCGGGTCTGGTGGGTGGTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTA  >  NC_000913/1627840‑1628102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑