Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,680,476	T→C	V307A (GTA→GCA)	ydgI →	putative arginine:ornithine antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,680,476	0	T	C	100.0%	131.3 / NA	37	V307A (GTA→GCA)	ydgI	putative arginine:ornithine antiporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/16);  total (21/16)

GCTGAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACC  >  NC_000913/1680340‑1680609                                                                                                                                         |                                                                                                                                      gCTGAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCAcc                                                                                                                                    >  2:758250/1‑140 (MQ=255) gCTGAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCAcc                                                                                                                                    >  1:236181/1‑140 (MQ=255)         tCGTAACCCGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTgg                                                                                                                            <  2:960455/140‑1 (MQ=255)             aaCCCGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGgccgc                                                                                                                        <  2:193275/140‑1 (MQ=255)              aCCCGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGgccgcc                                                                                                                       <  2:236181/140‑1 (MQ=255)               cccGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCa                                                                                                                      <  2:328874/140‑1 (MQ=255)                   tCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCa                                                                                                                  >  2:351419/1‑140 (MQ=255)                    cAATGGCTGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCat                                                                                                                     >  2:475443/1‑135 (MQ=255)                    cAATGGCTGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCat                                                                                                                     <  1:475443/136‑2 (MQ=255)                         gCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGc                                                                                                            >  1:101647/1‑140 (MQ=255)                             gTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAATCACCGTTCCTGGCCGCCATTCATAAAGCATTc                                                                                                        >  1:95530/1‑140 (MQ=255)                             gTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCatt                                                                                                                    >  1:151736/1‑126 (MQ=255)                             gTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCatt                                                                                                                    <  2:151736/128‑3 (MQ=255)                                 gATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTccc                                                                                                      >  2:821929/1‑138 (MQ=255)                                 gATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTccc                                                                                                      <  1:821929/138‑1 (MQ=255)                                   tggtggAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCgcgc                                                                                                  >  1:629224/1‑140 (MQ=255)                                     gtggAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCAt                                                                                                <  1:182509/140‑1 (MQ=255)                                            tgatgGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTgcg                                                                                         >  2:288133/1‑140 (MQ=255)                                              atgGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTgcgcg                                                                                       >  2:372211/1‑140 (MQ=255)                                                        ggggCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGca                                                                             >  2:823861/1‑140 (MQ=255)                                                               aaTCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTg                                                                      >  1:255568/1‑140 (MQ=255)                                                                     catcGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCAt                                                                <  1:1006088/140‑1 (MQ=255)                                                                     catcGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCAt                                                                <  2:629224/140‑1 (MQ=255)                                                                         gCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGc                                                            <  2:380872/140‑1 (MQ=255)                                                                             ccGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCa                                                        >  1:701877/1‑140 (MQ=255)                                                                              cGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCAc                                                       <  1:288133/140‑1 (MQ=255)                                                                                           tCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCaa                                          >  1:458295/1‑140 (MQ=255)                                                                                                ttGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCt                                     <  1:758250/140‑1 (MQ=255)                                                                                                               aaGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTc                      <  2:201255/140‑1 (MQ=255)                                                                                                                     gACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGTGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTAc                >  1:758839/1‑140 (MQ=255)                                                                                                                       ccATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTc              >  2:905206/1‑140 (MQ=255)                                                                                                                          tcatGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATc           <  2:255568/140‑1 (MQ=255)                                                                                                                           catGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCt          >  1:925710/1‑140 (MQ=255)                                                                                                                               gcagcaGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCt      >  1:862175/1‑140 (MQ=255)                                                                                                                                  gcagAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGa    >  2:303338/1‑139 (MQ=255)                                                                                                                                  gcagAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGa    <  1:303338/139‑1 (MQ=255)                                                                                                                                   cagAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGAcc  >  2:727418/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      GCTGAAATTCGTAACCCGTCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACC  >  NC_000913/1680340‑1680609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑