Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,680,494	C→T	T313I (ACT→ATT)	ydgI →	putative arginine:ornithine antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,680,494	0	C	T	100.0%	110.9 / NA	32	T313I (ACT→ATT)	ydgI	putative arginine:ornithine antiporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/13);  total (19/13)

TCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACA  >  NC_000913/1680358‑1680625                                                                                                                                         |                                                                                                                                    tCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCa                                                                                                                                  >  2:351419/1‑140 (MQ=255)       gCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGc                                                                                                                            >  1:101647/1‑140 (MQ=255)           gTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAATCACCGTTCCTGGCCGCCATTCATAAAGCATTc                                                                                                                        >  1:95530/1‑140 (MQ=255)               gATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTccc                                                                                                                      >  2:821929/1‑138 (MQ=255)               gATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTccc                                                                                                                      <  1:821929/138‑1 (MQ=255)                 tggtggAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCgcgc                                                                                                                  >  1:629224/1‑140 (MQ=255)                   gtggAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCAt                                                                                                                <  1:182509/140‑1 (MQ=255)                          tgatgGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTgcg                                                                                                         >  2:288133/1‑140 (MQ=255)                            atgGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTgcgcg                                                                                                       >  2:372211/1‑140 (MQ=255)                                      ggggCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGca                                                                                             >  2:823861/1‑140 (MQ=255)                                             aaTCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTg                                                                                      >  1:255568/1‑140 (MQ=255)                                                   catcGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCAt                                                                                <  2:629224/140‑1 (MQ=255)                                                   catcGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCAt                                                                                <  1:1006088/140‑1 (MQ=255)                                                       gCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGc                                                                            <  2:380872/140‑1 (MQ=255)                                                           ccGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCa                                                                        >  1:701877/1‑140 (MQ=255)                                                            cGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCAc                                                                       <  1:288133/140‑1 (MQ=255)                                                                         tCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCaa                                                          >  1:458295/1‑140 (MQ=255)                                                                              ttGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCt                                                     <  1:758250/140‑1 (MQ=255)                                                                                             aaGCTGGACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTc                                      <  2:201255/140‑1 (MQ=255)                                                                                                   gACCATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGTGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTAc                                >  1:758839/1‑140 (MQ=255)                                                                                                     ccATCATGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTc                              >  2:905206/1‑140 (MQ=255)                                                                                                        tcatGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATc                           <  2:255568/140‑1 (MQ=255)                                                                                                         catGGCAGCAGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCt                          >  1:925710/1‑140 (MQ=255)                                                                                                             gcagcaGAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCt                      >  1:862175/1‑140 (MQ=255)                                                                                                                gcagAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGa                    >  2:303338/1‑139 (MQ=255)                                                                                                                gcagAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGa                    <  1:303338/139‑1 (MQ=255)                                                                                                                 cagAAGCACCGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGAcc                  >  2:727418/1‑140 (MQ=255)                                                                                                                       ccccGTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTcc            <  2:101647/138‑1 (MQ=255)                                                                                                                           gTTCCTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGAtt        >  2:685160/1‑140 (MQ=255)                                                                                                                              ccTGGCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTaca     >  2:377081/1‑140 (MQ=255)                                                                                                                                 ggCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTacaaca  <  1:82319/140‑1 (MQ=255)                                                                                                                                 ggCCGCCATTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTacaaca  <  1:685160/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    TCAATGGCCGGTCTGATGGTGGAAATGATGGGACCATGGGGCGAAATCATCATCGCTGCCGGTTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACA  >  NC_000913/1680358‑1680625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑