Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	179,722	A→G	L162L (TTA→TTG)	dgt →	dGTP triphosphohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	179,722	0	A	G	100.0%	172.0 / NA	46	L162L (TTA→TTG)	dgt	dGTP triphosphohydrolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/26);  total (20/26)

GATATCGGCAATCCGCCGTTTGGTCATTTTGGCGAAGCGGCGATAAATGACTGGTTTCGCCAACGTTTGCACCCGGAAGATGCCGAAAGCCAGCCTCTGACTGACGATCGCTGCAGCGTGGCGGCACTACGTTTACGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATACATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCG  >  NC_000913/179588‑179858                                                                                                                                       |                                                                                                                                         gATATCGGCAATCCGCCGTTTGGTCATTTTGGCGAAGCGGCGATAAATGACTGGTTTCGCCAACGTTTGCACCCGGAAGATGCCGAAAGCCAGCCTCTGACTGACGATCGCTGCAGCGTGGCGGCACTACGTTTgcggga                                                                                                                                     <  1:481515/140‑1 (MQ=255) gATATCGGCAATCCGCCGTTTGGTCATTTTGGCGAAGCGGCGATAAATGACTGGTTTCGCCAACGTTTGCACCCGGAAGATGCCGAAAGCCAGCCTCTGACTGACGATCGCTGCAGCGTGGCGGCACTACGTTTgcggga                                                                                                                                     <  2:37010/140‑1 (MQ=255) gATATCGGCAATCCGCCGTTTGGTCATTTTGGCGAAGCGGCGATAAATGACTGGTTTCGCCAACGTTTGCACCCGGAAGATGCCGAAAGCCAGCCTCTGACTGACGATCGCTGCAGCGTGGCGGCACTACGTTTgcggga                                                                                                                                     <  1:308685/140‑1 (MQ=255)   tatCGGCAATCCGCCGTTTGGTCATTTTGGCGAAGCGGCGATAAATGACTGGTTTCGCCAACGTTTGCACCCGGAAGATGCCGAAAGCCAGCCTCTGACTGACGATCGCTGCAGCGTGGCGGCACTACGTTTgcgggacg                                                                                                                                   >  1:917256/1‑140 (MQ=255)    atCGGCAATCCGCCGTTTGGTCATTTTGGCGAAGCGGCGATAAATGACTGGTTTCGCCAACGTTTGCACCCGGAAGATGCCGAAAGCCAGCCTCTGACTGACGATCGCTGCAGCGTGGCGGCACTACGTTTgcgggacgg                                                                                                                                  >  2:448704/1‑140 (MQ=255)             ccgccgTTTGGTCATTTTGGCGAAGCGGCGATAAATGACTGGTTTCGCCAACGTTTGCACCCGGAAGATGCCGAAAGCCAGCCTCTGACTGACGATCGCTGCAGCGTGGCGGCACTACGTTTGCGGGACGGGGAAGAAcc                                                                                                                         >  1:889275/1‑140 (MQ=255)                      ggTCATTTTGGCGAAGCGGCGATAAATGACTGGTTTCGCCAACGTTTGCACCCGGAAGATGCCGAAAGCCAGCCTCTGACTGACGATCGCTGCAGCGTGGCGGCACTACGTTTGCGGGACGGGGAAGAACCGCTTAACGa                                                                                                                >  2:877914/1‑140 (MQ=255)                          aTTTTGGCGAAGCGGCGATAAATGACTGGTTTCGCCAACGTTTGCACCCGGAAGATGCCGAAAGCCAGCCTCTGACTGACGATCGCTGCAGCGTGGCGGCACTACGTTTGCGGGACGGGGAAGAACCGCTTAACGAGCTg                                                                                                            <  2:805825/140‑1 (MQ=255)                             ttGGCGAAGCGGCGATAAATGACTGGTTTCGCCAACGTTTGCACCCGGAAGATGCCGAAAGCCAGCCTCTGACTGACGATCGCTGCAGCGTGGCGGCACTACGTTTGCGGGACGGGGAAGAACCGCTTAACGAGCTgcgg                                                                                                         <  2:319687/140‑1 (MQ=255)                               ggCGAAGCGGCGATAAATGACTGGTTTCGCCAACGTTTGCACCCGGAAGATGCCGAAAGCCAGCCTCTGACTGACGATCGCTGCAGCGTGGCGGCACTACGTTTGCGGGACGGGGAAGAACCGCTTAACGAGCTgcggcg                                                                                                       <  2:28153/140‑1 (MQ=255)                                      cggcgATAAATGACTGGTTTCGCCAACGTTTGCACCCGGAAGATGCCGAAAGCCAGCCTCTGACTGACGATCGCTGCAGCGTGGCGGCACTACGTTTGCGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGAtt                                                                                                >  2:140928/1‑140 (MQ=255)                                          gATAAATGACTGGTTTCGCCAACGTTTGCACCCGGAAGATGCCGAAAGCCAGCCTCTGACTGACGATCGCTGCAGCGTGGCGGCACTACGTTTGCGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTc                                                                                            <  2:682110/140‑1 (MQ=255)                                                    tGGTTTCGCCAACGTTTGCACCCGGAAGATGCCGAAAGCCAGCCTCTGACTGACGATCGCTGCAGCGTGGCGGCACAACGTTTGCGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATg                                                                                  <  2:1055927/140‑1 (MQ=255)                                                     ggTTTCGCCAACGTTTGCACCCGGAAGATGCCGAAAGCCAGCCTCTGACTGACGATCGCTGCAGCGTGGCGGCACTACGTTTGCGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGt                                                                                 >  1:838400/1‑140 (MQ=255)                                                      gTTTCGCCAACGTTTGCACCCGGAAGATGCCGAAAGCCAGCCTCTGACTGACGATCGCTGCAGCGTGGCGGCACTACGTTTGCGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTc                                                                                >  2:1020843/1‑140 (MQ=255)                                                      gTTTCGCCAACGTTTGCACCCGGAAGATGCCGAAAGCCAGCCTCTGACTGACGATCGCTGCAGCGTGGCGGCACTACGTTTGCGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTc                                                                                >  1:978752/1‑140 (MQ=255)                                                       tttCGCCAACGTTTGCACCCGGAAGATGCCGAAAGCCAGCCTCTGACTGACGATCGCTGCAGCGTGGCGGCACTACGTTTGCGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCa                                                                               <  1:785671/140‑1 (MQ=255)                                                           gCCAACGTTTGCACCCGGAAGATGCCGAAAGCCAGCCTCTGACTGACGATCGCTGCAGCGTGGCGGCACTACGTTTGCGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCAtttt                                                                           <  1:980460/140‑1 (MQ=255)                                                            ccAACGTTTGCACCCGGAAGATGCCGAAAGCCAGCCTCTGACTGACGATCGCTGCAGCGTGGCGGCACTACGTTTGCGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTg                                                                          <  2:399082/140‑1 (MQ=255)                                                             cAACGTTTGCACCCGGAAGATTCCGAAAGCCAGCCTCTGACTGACGATCGCTGCAGCGTGGCGGCACTACGTTTGCGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGa                                                                         <  2:734106/140‑1 (MQ=255)                                                             cAACGTTTGCACCCGGAAGATGCCGAAAGCCAGCCTCTGACTGACGATCGCTGCAGCGTGGCGGCACTACGTTTGCGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGa                                                                         <  1:1020843/140‑1 (MQ=255)                                                             cAACGTTTGCACCCGGAAGATGCCGAAAGCCAGCCTCTGACTGACGATCGCTGCAGCGTGGCGGCACTACGTTTGCGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGa                                                                         <  2:978752/140‑1 (MQ=255)                                                                 gTTTGCACCCGGAAGATGCCGAAAGCCAGCCTCTGACTGACGATCGCTGCAGCGTGGCGGCACTACGTTTGCGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAgggg                                                                     <  2:189589/140‑1 (MQ=255)                                                                  tttGCACCCGGAAGATGCCGAAAGCCAGCCTCTGACTGACGATCGCTGCAGCGTGGCGGCACTACGTTTGCGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGa                                                                    <  1:895300/140‑1 (MQ=255)                                                                  tttGCACCCGGAAGATGCCGAAAGCCAGCCTCTGACTGACGATCGCTGCAGCGTGGCGGCACTACGTTTGCGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGa                                                                    <  2:921876/140‑1 (MQ=255)                                                                  tttGCACCCGGAAGATGCCGAAAGCCAGCCTCTGACTGACGATCGCTGCAGCGTGGCGGCACTACGTTTGCGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGa                                                                    <  1:745147/140‑1 (MQ=255)                                                                         ccGGAAGATGCCGAAAGCCAGCCTCTGACTGACGATCGCTGCAGCGTGGCGGCACTACGTTTGCGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGcaca                                                             >  1:791405/1‑140 (MQ=255)                                                                           ggAAGATGCCGAAAGCCAGCCTCTGACTGACGATCGCTGCAGCGTGGCGGCACTACGTTTGCGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAg                                                           >  2:163670/1‑140 (MQ=255)                                                                                aTGCCGAAAGCCAGCCTCTGACTGACGATCGCTGCAGCGTGGCGGCACTACGTTTGCGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCAtt                                                      >  2:270971/1‑140 (MQ=255)                                                                                 tGCCGAAAGCCAGCCTCTGACTGACGATCGCTGCAGCGTGGCGGCACTACGTTTGCGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGaa                                                                   <  1:576482/126‑1 (MQ=255)                                                                                 tGCCGAAAGCCAGCCTCTGACTGACGATCGCTGCAGCGTGGCGGCACTACGTTTGCGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGaa                                                                   >  2:576482/1‑126 (MQ=255)                                                                                 tGCCGAAAGCCAGCCTCTGACTGACGATCGCTGCAGCGTGGCGGCACTACGTTTGCGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTc                                                     >  1:272480/1‑140 (MQ=255)                                                                                   ccGAAAGCCAGCCTCTGACTGACGATCGCTGCAGCGTGGCGGCACTACGTTTGCGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGc                                                   <  1:163670/140‑1 (MQ=255)                                                                                    cGAAAGCCAGCCTCTGACTGACGATCGCTGCAGCGTGGCGGCACTACGTTTGCGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGcc                                                  <  2:889275/140‑1 (MQ=255)                                                                                                   gactgacGATCGCTGCAGCGTGGCGGCACTACGTTTGCGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATACATTGa                                   >  1:377032/1‑140 (MQ=255)                                                                                                    actgacGATCGCTGCAGCGTGGCGGCACTACGTTTGCGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTg                                                >  1:1046942/1‑126 (MQ=255)                                                                                                    actgacGATCGCTGCAGCGTGGCGGCACTACGTTTGCGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTg                                                <  2:1046942/126‑1 (MQ=255)                                                                                                        acGATCGCTGCAGCGTGGCGGCACTACGTTTGCGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATACATTGATGCgg                              >  1:195038/1‑140 (MQ=255)                                                                                                        acGATCGCTGCAGCGTGGCGGCACTACGTTTGCGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATACATTGATGCgg                              >  2:729455/1‑140 (MQ=255)                                                                                                        acGATCGCTGCAGCGTGGCGGCACTACGTTTGCGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATACATTGATGCgg                              >  1:816006/1‑140 (MQ=255)                                                                                                         cGATCGCTGCAGCGTGGCGGCACTACGTTTGCGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCGTTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATACATTGATGCGGa                             <  1:480054/140‑1 (MQ=255)                                                                                                         cGATCGCTGCAGCGTGGCGGCACTACGTTTGCGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATACATTGATGCGGa                             <  2:377032/140‑1 (MQ=255)                                                                                                         cGATCGCTGCAGCGTGGCGGCACTACGTTTGCGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATACATTGATGCGGa                             <  1:140928/140‑1 (MQ=255)                                                                                                          gATCGCTGCAGCGTGGCGGCACTACGTTTGCGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATACATTGATGCGGAt                            >  1:175592/1‑140 (MQ=255)                                                                                                                tGCAGCGTGGCGGCACTACGTTTGCGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATACATTGATGCGGATGAAtct                      >  1:923758/1‑140 (MQ=255)                                                                                                                tGCAGCGTGGCGGCACTACGTTTGCGGGACGGGGAAGAACCACTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATACATTGATGCGGATGAAtct                      >  2:531315/1‑140 (MQ=255)                                                                                                                          cggcACTACGTTTGCGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATACATTGATGCGGATGAATCTCACCTGGGca            <  2:838400/140‑1 (MQ=255)                                                                                                                          cggcACTACGTTTGCGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATACATTGATGCGGATGAATCTCACCTGGGca            <  1:877914/140‑1 (MQ=255)                                                                                                                            gcACTACGTTTGCGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATACATTGATGCGGATGAATCTCACCTGGGcaca          >  2:379916/1‑140 (MQ=255)                                                                                                                             cACTACGTTTGCGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATACATTGATGCGGATGAATCTCACCTGGGCACAg         <  2:791405/140‑1 (MQ=255)                                                                                                                                    tttGCGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATACATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCg  <  1:386500/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         GATATCGGCAATCCGCCGTTTGGTCATTTTGGCGAAGCGGCGATAAATGACTGGTTTCGCCAACGTTTGCACCCGGAAGATGCCGAAAGCCAGCCTCTGACTGACGATCGCTGCAGCGTGGCGGCACTACGTTTACGGGACGGGGAAGAACCGCTTAACGAGCTGCGGCGCAAGATTCGTCAGGACTTATGTCATTTTGAGGGGAATGCACAAGGCATTCGCCTGGTGCATACATTGATGCGGATGAATCTCACCTGGGCACAGGTTGGCG  >  NC_000913/179588‑179858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑