Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,797,060	C→T	A295A (GCG→GCA)	pheT ←	phenylalanine‑‑tRNA ligase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,797,060	0	C	T	100.0%	199.3 / NA	57	A295A (GCG→GCA)	pheT	phenylalanine‑‑tRNA ligase subunit beta
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/36);  total (21/36)

CGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGAC  >  NC_000913/1796925‑1797191                                                                                                                                        |                                                                                                                                    cGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAgctttgctt                                                                                                                                 >  2:1058662/1‑140 (MQ=255) cGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAgctttgctt                                                                                                                                 >  2:682854/1‑140 (MQ=255)         aGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTTGCTTCAGTACCg                                                                                                                         <  2:267897/140‑1 (MQ=255)          gAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTTGCTTCAGTACCGt                                                                                                                        <  1:1058662/140‑1 (MQ=255)          gAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTTGCTTCAGTACCGt                                                                                                                        <  1:682854/140‑1 (MQ=255)             cgcgCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTTGCTTCAGTACCGTCGa                                                                                                                     >  1:830591/1‑140 (MQ=255)             cgcgCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTTGCTTCAGTACCGTCGa                                                                                                                     >  1:260460/1‑140 (MQ=255)             cgcgCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTTGCTTCAGTACCGTCGa                                                                                                                     >  1:260461/1‑140 (MQ=255)              gcgcATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTTGCTTCAGTACCGTCGag                                                                                                                    <  2:112574/140‑1 (MQ=255)              gcgcATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTTGCTTCAGTACCGTCGag                                                                                                                    >  2:444426/1‑140 (MQ=255)              gcgcATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTTGCTTCAGTACCGTCGag                                                                                                                    <  2:388814/140‑1 (MQ=255)                              cGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTTGCTTCAGTACCGTCGAgcagca                                                                                                               >  1:311024/1‑129 (MQ=255)                              cGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTTGCTTCAGTACCGTCGAgcagca                                                                                                               <  2:311024/129‑1 (MQ=255)                              cGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTTGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCg                                                                                                    >  1:53236/1‑140 (MQ=255)                              cGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTTGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCg                                                                                                    >  1:322142/1‑140 (MQ=255)                              cGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTTGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCg                                                                                                    >  2:905961/1‑140 (MQ=255)                              cGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTTGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCg                                                                                                    <  2:805038/140‑1 (MQ=255)                              cGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTTGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCg                                                                                                    >  2:39157/1‑140 (MQ=255)                                     tgtTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTTGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCtt                                                                                             <  2:830591/140‑1 (MQ=255)                                        ttCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTTGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCg                                                                                          <  1:39157/140‑1 (MQ=255)                                          cGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTTGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGcc                                                                                        <  2:126550/140‑1 (MQ=255)                                          cGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTTGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGcc                                                                                        <  1:33653/140‑1 (MQ=255)                                                 cACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTTGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCGca                                                                                 <  1:905961/140‑1 (MQ=255)                                                   cGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTTGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCGcacc                                                                               <  2:722899/140‑1 (MQ=255)                                                          gtgtTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTTGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATgc                                                                        <  2:783216/140‑1 (MQ=255)                                                              tCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTTGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCGCACcaca                                                                            >  1:587428/1‑132 (MQ=255)                                                              tCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTTGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCGCACcaca                                                                            <  2:587428/132‑1 (MQ=255)                                                              tCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTTGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATgccgcc                                                                    >  2:880629/1‑140 (MQ=255)                                                              tCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTTGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATgccgcc                                                                    >  2:1009227/1‑140 (MQ=255)                                                                gCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTTGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCtt                                                                  <  1:444426/140‑1 (MQ=255)                                                                       aaGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTTGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCg                                                           >  1:464324/1‑140 (MQ=255)                                                                        aGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTTGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGa                                                          <  2:260460/140‑1 (MQ=255)                                                                        aGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTTGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGa                                                          <  2:260461/140‑1 (MQ=255)                                                                              cgccCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTTGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTa                                                    <  1:1009227/140‑1 (MQ=255)                                                                                 ccATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTTGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCg                                                 <  1:114592/140‑1 (MQ=255)                                                                                    tCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTTGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGaaa                                              >  1:636555/1‑140 (MQ=255)                                                                                         aGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTTGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTg                                         <  2:464324/140‑1 (MQ=255)                                                                                               ttgttgTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTTGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCgg                                   <  1:1018970/140‑1 (MQ=255)                                                                                               ttgttgTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTTGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCgg                                   <  1:802091/140‑1 (MQ=255)                                                                                                tgttgtGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTTGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGc                                  >  1:764417/1‑140 (MQ=255)                                                                                                    gtgGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTTGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGc                              >  1:502041/1‑140 (MQ=255)                                                                                                    gtgGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTTGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGc                              <  2:53236/140‑1 (MQ=255)                                                                                                    gtgGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTTGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGc                              <  2:637364/140‑1 (MQ=255)                                                                                                          ggCGATGACCAGAGTGTCAGCATTCAGCTTTGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCgg                                   <  1:903498/129‑1 (MQ=255)                                                                                                          ggCGATGACCAGAGTGTCAGCATTCAGCTTTGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCgg                                   >  2:903498/1‑129 (MQ=255)                                                                                                          ggCGATGACCAGAGTGTCAGCATTCAGCTTTGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGc                              >  2:1040209/1‑134 (MQ=255)                                                                                                          ggCGATGACCAGAGTGTCAGCATTCAGCTTTGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGc                              <  1:1040209/134‑1 (MQ=255)                                                                                                           gCGATGACCAGAGTGTCAGCATTCAGCTTTGCTTCAGTACCGTCGAGCAGCCCCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTc                       <  1:491268/140‑1 (MQ=255)                                                                                                              aTGACCAGAGTGTCAGCATTCAGCTTTGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGag                    <  2:322142/140‑1 (MQ=255)                                                                                                              aTGACCAGAGTGTCAGCATTCAGCTTTGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGag                    <  2:764417/140‑1 (MQ=255)                                                                                                              aTGACCAGAGTGTCAGCATTCAGCTTTGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGag                    <  1:824172/140‑1 (MQ=255)                                                                                                              aTGACCAGAGTGTCAGCATTCAGCTTTGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGag                    <  1:783381/140‑1 (MQ=255)                                                                                                              aTGACCAGAGTGTCAGCATTCAGCTTTGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGag                    <  2:904248/140‑1 (MQ=255)                                                                                                                 aCCAGAGTGTCAGCATTCAGCTTTGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGagcag                 >  1:888105/1‑140 (MQ=255)                                                                                                                  ccAGAGTGTCAGCATTCAGCTTTGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGagcagc                <  2:566704/140‑1 (MQ=255)                                                                                                                           cAGCATTCAGCTTTGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTg       >  2:960300/1‑140 (MQ=255)                                                                                                                             gCATTCAGCTTTGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGt     <  1:880629/140‑1 (MQ=255)                                                                                                                              cATTCAGCTTTGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTg    <  2:603663/140‑1 (MQ=255)                                                                                                                                ttCAGCTTTGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGAc  >  2:846122/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                    CGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGAC  >  NC_000913/1796925‑1797191

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑