Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,853,409	A→G	F191L (TTT→CTT)	ydjE ←	putative transporter YdjE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,853,409	0	A	G	100.0%	154.2 / NA	42	F191L (TTT→CTT)	ydjE	putative transporter YdjE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/20);  total (22/20)

AGTAAGCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAACCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGG  >  NC_000913/1853272‑1853537                                                                                                                                          |                                                                                                                                 aGTAAGCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTACACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGcc                                                                                                                                >  2:828644/1‑140 (MQ=255)     aGCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGc                                                                                                                            >  1:776188/1‑140 (MQ=255)      gCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGTCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCt                                                                                                                           <  2:323306/140‑1 (MQ=255)      gCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCt                                                                                                                           <  2:266737/140‑1 (MQ=255)      gCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCt                                                                                                                           <  1:668568/140‑1 (MQ=255)        gggggTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTaa                                                                                                                         <  1:350829/140‑1 (MQ=255)           ggTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAg                                                                                                                      >  2:984694/1‑140 (MQ=255)           ggTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAg                                                                                                                      <  2:347930/140‑1 (MQ=255)           ggTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAg                                                                                                                      <  2:776188/140‑1 (MQ=255)            gTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGt                                                                                                                     <  1:70166/140‑1 (MQ=255)                 cGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGcc                                                                                                                >  1:993793/1‑140 (MQ=255)                   aaTACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCaa                                                                                                              <  1:975085/140‑1 (MQ=255)                     tACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATa                                                                                                            <  1:212030/140‑1 (MQ=255)                     tACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATa                                                                                                            <  2:485209/140‑1 (MQ=255)                     tACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATa                                                                                                            <  2:550669/140‑1 (MQ=255)                         cttctCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATaccac                                                                                                        <  2:993793/140‑1 (MQ=255)                          ttctCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATaccacc                                                                                                       <  1:830058/140‑1 (MQ=255)                          ttctCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATaccacc                                                                                                       >  1:769647/1‑140 (MQ=255)                          ttctCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATaccacc                                                                                                       >  1:697558/1‑140 (MQ=255)                          ttctCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATaccacc                                                                                                       >  2:360078/1‑140 (MQ=255)                           tctctcTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATACCAccc                                                                                                      <  2:94120/140‑1 (MQ=255)                                ctTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATACCACCcagcag                                                                                                 >  2:189137/1‑140 (MQ=255)                                  ttCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATACCACCCAGCAGaa                                                                                               >  1:610693/1‑140 (MQ=255)                                    cAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCCAACAGTATGCCAATACCACCCAGCAGAAAc                                                                                             >  2:757344/1‑140 (MQ=255)                                                     tCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTaaa                                                                            >  2:1049722/1‑140 (MQ=255)                                                          aaGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAg                                                                       <  2:610693/140‑1 (MQ=255)                                                              tGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGAt                                                                   <  2:598367/140‑1 (MQ=255)                                                                            tGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTa                                                     <  1:708312/140‑1 (MQ=255)                                                                             gCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTAt                                                    >  1:579995/1‑140 (MQ=255)                                                                              cGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATc                                                   >  1:1000192/1‑140 (MQ=255)                                                                              cGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATc                                                   >  2:1048885/1‑140 (MQ=255)                                                                              cGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATc                                                   >  2:497735/1‑140 (MQ=255)                                                                                  ttGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATcgccg                                               <  1:202929/140‑1 (MQ=255)                                                                                  ttGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATcgccg                                               <  1:670131/140‑1 (MQ=255)                                                                                          tCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGc                                       >  2:1059371/1‑140 (MQ=255)                                                                                           cccTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCa                                      >  2:174475/1‑140 (MQ=255)                                                                                               gccagccaTCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATggg                                  >  1:918672/1‑140 (MQ=255)                                                                                                        cGTGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGtt                         >  2:675846/1‑140 (MQ=255)                                                                                                          tGGCGACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTAc                       <  1:984694/140‑1 (MQ=255)                                                                                                              gACTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACcaac                   >  2:1032006/1‑140 (MQ=255)                                                                                                               aCTCGATAAAGTATTTACCTGAGAGAAGCCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACcaaca                  >  2:728241/1‑140 (MQ=255)                                                                                                                               aCCTGAGAGAAGCCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCgg  >  1:883493/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                 AGTAAGCGGGGGTAAACGAATACTCTTCTCTCTTTCAATTTGCTGCTCTACTTCACGAAGTTGGCATTCTGCACCTGCGATTTGCCCTTTCCCTGCCAGCCATCGTGGCGACTCGATAAAGTATTTACCTGAGAGAAACCAGGCTAACAGTATGCCAATACCACCCAGCAGAAACATTATTCGCCAACTAAAAAAAGCGATAACCACCACGCCTATCGCCGCAGACAGCATGGGCGACCAGTTACCAACAAATGAGAGCCGCGCGG  >  NC_000913/1853272‑1853537

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑