Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,875,472	T→C	V35V (GTA→GTG)	nimR ←	DNA‑binding transcriptional repressor NimR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,875,472	0	T	C	100.0%	179.2 / NA	48	V35V (GTA→GTG)	nimR	DNA‑binding transcriptional repressor NimR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/23);  total (25/23)

TACTTGATTGCTATGTTCCACCCCGCCCGGTATCCAAACCGCATATTGTGGCGGCACCATCCACAAAGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGTACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGGTGTCGGTCGGGTTCATAGCCATTGAGATTCAACCTGTGCATCATTTTGTCCGAACTTAGCGATAATTTGTC  >  NC_000913/1875337‑1875603                                                                                                                                        |                                                                                                                                    tACTTGATTGCTATGTTCCACCCCGCCCGGTATCCAAACCGCATATTGTGGCGGCACCATCCACAAAGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAg                                                                                                                                 >  2:14114/1‑140 (MQ=255)  aCTTGATTGCTATGTTCCACCCCGCCCGGTATCCAAACCGCATATTGTGGCGGCACCATCCACAAAGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAgg                                                                                                                                <  1:538148/140‑1 (MQ=255)   cTTGATTGCTATGTTCCACCCCGCCCGGTATCCAAACCGCATATTGTGGCGGCACCATCCACAAAGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGa                                                                                                                               <  2:114477/140‑1 (MQ=255)   cTTGATTGCTATGTTCCACCCCGCCCGGTATCCAAACCGCATATTGTGGCGGCACCATCCACAAAGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGa                                                                                                                               <  1:247261/140‑1 (MQ=255)       aTTGCTATGTTCCACCCCGCCCGGTATCCAAACCGCATATTGTGGCGGCACCATCCACAAAGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGa                                                                                                                               >  2:95378/1‑136 (MQ=255)       aTTGCTATGTTCCACCCCGCCCGGTATCCAAACCGCATATTGTGGCGGCACCATCCACAAAGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGa                                                                                                                               <  1:95378/136‑1 (MQ=255)       aTTGCTATGTTCCACCCCGCCCGGTATCCAAACCGCATATTGTGGCGGCACCATCCACAAAGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTgg                                                                                                                           <  2:846734/140‑1 (MQ=255)          gCTATGTTCCACCCCGCCCGGTATCCAAACCGCATATTGTGGCGGCACCATCCACAAAGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTa                                                                                                                        >  2:1039342/1‑140 (MQ=255)                ttCCACCCCGCCCGGTATCCAAACCGCATATTGTGGCGGCACCATCCACAAAGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCAt                                                                                                                  <  2:639935/140‑1 (MQ=255)                ttCCACCCCGCCCGGTATCCAAACCGCATATTGTGGCGGCACCATCCACAAAGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCAt                                                                                                                  <  1:14114/140‑1 (MQ=255)                 tCCACCCCGCCCGGTATCCAAACCGCATATTGTGGCGGCACCATCCACAAAGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATc                                                                                                                 >  1:91747/1‑140 (MQ=255)                     ccccGCCCGGTATCCAAACCGCATATTGTGGCGGCACCATCCACAAAGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGa                                                                                                                               <  2:211607/122‑1 (MQ=255)                     ccccGCCCGGTATCCAAACCGCATATTGTGGCGGCACCATCCACAAAGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGa                                                                                                                               >  1:211607/1‑122 (MQ=255)                     ccccGCCCGGTATCCAAACCGCATATTGTGGCGGCACCATCCACAAAGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGtt                                                                                                             <  1:233858/140‑1 (MQ=255)                         gcccgGTATCCAAACCGCATATTGTGGCGGCACCATCCACAAAGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCgg                                                                                                         <  1:560980/140‑1 (MQ=255)                                         gCATATTGTGGCGGCACCATCCACAAAGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGc                                                                                         >  1:89780/1‑140 (MQ=255)                                           atatTGTGGCGGCACCATCCACAAAGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAGCTCATCCGTTCCGGCGTGAATACAAAATGCGa                                                                                       >  1:341275/1‑140 (MQ=255)                                              ttGTGGCGGCACCATCCACAAAGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACcg                                                                                    <  2:823429/140‑1 (MQ=255)                                                gtgGCGGCACCATCCACAAAGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACcgcg                                                                                  <  1:1039342/140‑1 (MQ=255)                                                gtgGCGGCACCATCCACAAAGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACcgcg                                                                                  <  1:750300/140‑1 (MQ=255)                                                 tgGCGGCACCATCCACAAAGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCgg                                                                                 >  1:228960/1‑140 (MQ=255)                                                  ggcggcACCATCCACAAAGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGc                                                                                >  2:772908/1‑140 (MQ=255)                                                                 aaaGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCGGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGGTgtcggtc                                                                 <  2:802885/140‑1 (MQ=255)                                                                   aGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGgtgt                                                                      <  1:664544/133‑1 (MQ=255)                                                                   aGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGGTGtcggtcgg                                                               >  1:231370/1‑140 (MQ=255)                                                                   aGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCATTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGgtgt                                                                      >  2:664544/1‑133 (MQ=255)                                                                       ttttCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGGTGTCGGTCGGGTTc                                                           >  2:280461/1‑140 (MQ=255)                                                                        tttCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGGTGTCGGTCGGGTTCa                                                          <  2:89780/140‑1 (MQ=255)                                                                           ccACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGGTGTCGGTCGGGTTCATAg                                                       >  1:494214/1‑140 (MQ=255)                                                                            cACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGGTGTCGGTCGGGTTCATAGc                                                      <  2:414969/140‑1 (MQ=255)                                                                               cGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGGTGTCGGTCGGGTTCATAGCCAt                                                   <  2:494214/140‑1 (MQ=255)                                                                                gTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGGTGTCGGTCGGGTTCATAGCCAtt                                                  >  1:459962/1‑140 (MQ=255)                                                                                 tACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGGTGTCGGTCGGGTTCATAGCCATTg                                                 >  2:324455/1‑140 (MQ=255)                                                                                 tACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGGTGTCGGTCGGGTTCATAGCCATTg                                                 >  2:693744/1‑140 (MQ=255)                                                                                         aTTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGGTGTCGGTCGGGTTCATAGCCATTGAGATTCaa                                         >  1:700056/1‑140 (MQ=255)                                                                                          ttGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGGTGTCGGTCGGGTTCATAGCCATTGAGATTCAAc                                        <  2:91747/140‑1 (MQ=255)                                                                                           tGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGGTGTCGGTCGGGTTCATAGCCATTgaga                                              >  1:459316/1‑133 (MQ=255)                                                                                           tGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGGTGTCGGTCGGGTTCATAGCCATTgaga                                              <  2:459316/133‑1 (MQ=255)                                                                                           tGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGGTGTCGGTCGGGTTCATAGCCATTGAGATTCAAcc                                       >  2:535942/1‑140 (MQ=255)                                                                                                  tGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGGTGTCGGTCGGGTTCATAGCCATTGAGATTCAACCTGTGcat                                >  2:17263/1‑140 (MQ=255)                                                                                                      gcgcCAGTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGGTGTCGGTCGGGTTCATAGCCATTGAGATTCAACCTGTGCATCAtt                            <  2:228960/140‑1 (MQ=255)                                                                                                            gTATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGGTGTCGGTCGGGTTCATAGCCATTGAGATTCAACCTGTGCATCATTTTGTcc                      >  2:416439/1‑140 (MQ=255)                                                                                                             tATCAGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGGTGTCGGTCGGGTTCATAGCCATTGAGATTCAACCTGTGCATCATTTTGTCCg                     >  1:956677/1‑140 (MQ=255)                                                                                                                 aGCTGTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGGTGTCGGTCGGGTTCATAGCCATTGAGATTCAACCTGTGCATCATTTTGTCCGAACt                 >  1:444820/1‑140 (MQ=255)                                                                                                                     gTCCTTTGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGGTGTCGGTCGGGTTCATAGCCATTGAGATTCAACCTGTGCATCATTTTGTCCGAACTTAGc             <  1:416439/140‑1 (MQ=255)                                                                                                                           tGCGGTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGGTGTCGGTCGGGTTCATAGCCATTGAGATTCAACCTGTGCATCATTTTGTCCGAACTTAGCGATAAt       >  2:539363/1‑140 (MQ=255)                                                                                                                              ggTGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGGTGTCGGTCGGGTTCATAGCCATTGAGATTCAACCTGTGCATCATTTTGTCCGAACTTAGCGATAATTTg    <  2:622721/140‑1 (MQ=255)                                                                                                                                tGCTGATGCACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGGTGTCGGTCGGGTTCATAGCCATTGAGATTCAACCTGTGCATCATTTTGTCCGAACTTAGCGATAATTTGTc  >  2:368055/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                    TACTTGATTGCTATGTTCCACCCCGCCCGGTATCCAAACCGCATATTGTGGCGGCACCATCCACAAAGCATTTTCCACCGTACAGGTAATTGCACCATGTAGCGCCAGTATCAGCTGTCCTTTGCGGTGCTGATGTACAGGACTGGTTAACTCATCCGTTCCGGCGTGAATACAAAATGCGACCGCGGCTTCATGGTGTCGGTCGGGTTCATAGCCATTGAGATTCAACCTGTGCATCATTTTGTCCGAACTTAGCGATAATTTGTC  >  NC_000913/1875337‑1875603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑