Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	233,193	C→T	D255N (GAT→AAT)	mltD ←	membrane‑bound lytic murein transglycosylase D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	233,193	0	C	T	100.0%	175.3 / NA	49	D255N (GAT→AAT)	mltD	membrane‑bound lytic murein transglycosylase D
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/22);  total (27/22)

ACTTGCGCCCAGCGTGGAGCCTTTTACGCCA‑GCGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTACTGCTTCGTTTCCTGCGGCAACGGTAACGACCAGAAGTCCGTGGATTTCCCACGCGCTTTG  >  NC_000913/233056‑233329                                                                                                                                           |                                                                                                                                         aCTTGCGCCCAGCGTGGAGCCTTTTACGCCA‑GCGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATTgg                                                                                                                                        >  1:907009/1‑140 (MQ=255)         cctgCGTGGCG‑CTTTTACCCCAGGCTTTAAATGTCTTCATCTTGCTGAAGAAACCCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATTGGTCGTTGGc                                                                                                                                <  2:184944/137‑1 (MQ=255)                ggatctttttCCGCCG‑GCTTTGATTGTCTTCAGCTTGCTGACGGAACCCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATTGGTCGTTGGCAGACGTa                                                                                                                         <  2:455452/134‑1 (MQ=255)                ggAGCTTTTTCCGCCA‑GTGTTGATTTTTTTCAGCTTGCGGACGAAACCCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATTGGTCGTTGGCAGACGTa                                                                                                                         <  2:221617/140‑1 (MQ=255)                      ttttCCGCCG‑GGGTTGATTTTCTTCTGTTTGCGGACGAAACCCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATTGGTCGTTGGCAGACGTACGCCAt                                                                                                                   <  2:227792/140‑1 (MQ=255)                       tttACGCCA‑GCGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATTGGTCGTTGGCAGACGTACGCCATa                                                                                                                  >  2:952013/1‑140 (MQ=255)                                 gCGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATTGGTCGTTGGCAGACGTACGCCATAACGCTTGCt                                                                                                         >  1:950179/1‑140 (MQ=255)                                  cGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATTGGTCGTTGGCAGACGTACGCCATAACGCTTGCTg                                                                                                        >  1:380525/1‑140 (MQ=255)                                       aaTTTCTTCAGCTTGCTGACGAAACCCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATTGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGttttt                                                                                                   <  1:456387/140‑1 (MQ=255)                                            cTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATTGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGaa                                                                                              >  1:262490/1‑140 (MQ=255)                                             ttCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATTGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAat                                                                                             >  2:67481/1‑140 (MQ=255)                                               cAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATTGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAatat                                                                                           >  1:762698/1‑140 (MQ=255)                                                   ttccTGACGAAACCCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATTGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACt                                                                                       <  1:73784/137‑1 (MQ=255)                                                     gCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATTGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCa                                                                                     >  1:435548/1‑140 (MQ=255)                                                     gCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATTGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCa                                                                                     >  1:278821/1‑140 (MQ=255)                                                       tGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATTGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAAt                                                                                   >  1:25940/1‑140 (MQ=255)                                                           ggAAATCCCCGCCACATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATTGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCa                                                                               <  2:208516/140‑1 (MQ=255)                                                                   ccGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATTGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTa                                                                       <  2:189903/140‑1 (MQ=255)                                                                    cGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATTGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAg                                                                      <  1:952013/140‑1 (MQ=255)                                                                     gCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATTGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAgg                                                                     <  1:580478/140‑1 (MQ=255)                                                                      ccATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATTGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGc                                                                    <  1:245213/140‑1 (MQ=255)                                                                      ccATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATTGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGc                                                                    <  2:950179/140‑1 (MQ=255)                                                                         tatCTGCTGCCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATTGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGc                                                                    <  1:851560/137‑1 (MQ=255)                                                                         tatCTGCTGCCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATTGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGc                                                                    >  2:851560/1‑137 (MQ=255)                                                                                 aCCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATTGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTACTGCtt                                                         >  1:1051067/1‑140 (MQ=255)                                                                                     tCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATTGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTACTGCTTCGtt                                                     >  1:104346/1‑140 (MQ=255)                                                                                     tCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATTGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTACTGCTTCGtt                                                     >  1:16405/1‑140 (MQ=255)                                                                                     tCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATTGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTACTGCTTCGtt                                                     >  1:745878/1‑140 (MQ=255)                                                                                     tCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATTGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTACTGCTTCGtt                                                     >  1:182946/1‑140 (MQ=255)                                                                                     tCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATTGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTACTGCTTCGtt                                                     >  2:28070/1‑140 (MQ=255)                                                                                       gCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATTGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTACTGCTTCGTTTc                                                   >  2:67360/1‑140 (MQ=255)                                                                                                    ggCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATTGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTACTGCTTCGTTTCCTGCGGCAACGGt                                      >  2:362330/1‑140 (MQ=255)                                                                                                     gctgctCAGGTGCACACGCGCCAGAGCACGGCTTTCATTGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTACTGCTTCGTTTCCTGCGGCAACGGTa                                     <  2:862154/140‑1 (MQ=255)                                                                                                     gctgctCAGGTGCACACGCGCCAGAGCACGGCTTTCATTGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTACTGCTTCGTTTCCTGCGGCAACGGTa                                     >  1:318692/1‑140 (MQ=255)                                                                                                     gctgctCAGGTGCACACGCGCCAGAGCACGGCTTTCATTGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTACTGCTTCGTTTCCTGCGGCAACGGTa                                     <  2:380525/140‑1 (MQ=255)                                                                                                     gctgctCAGGTGCACACGCGCCAGAGCACGGCTTTCATTGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTACTGCTTCGTTTCCTGAGGCAACgata                                     <  2:262490/140‑4 (MQ=255)                                                                                                               tGCACACGCGCCAGAGCACGGCTTTCATTGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTACTGCTTCGTTTCCTGCGGCAACGGTAACGACCAGaa                           <  2:1002259/140‑1 (MQ=255)                                                                                                                   cacGCGCCAGAGCACGGCTTTCATTGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTACTGCTTCGTTTCCTGCGGCAACGGTAACGACCAGAAGTcc                       >  2:600420/1‑140 (MQ=255)                                                                                                                        gcCAGAGCACGGCTTTCATTGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTACTGCTTCGTTTCCTGCGGCAACGGTAACGACCAGAAGTCCGTGGa                  >  1:48756/1‑140 (MQ=255)                                                                                                                        gcCAGAGCACGGCTTTCATTGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTACTGCTTCGTTTCCTGCGGCAACGGTAACGACCAGAAGTCCGTGGa                  >  1:871040/1‑140 (MQ=255)                                                                                                                         ccAGAGCACGGCTTTCATTGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTACTGCTTCGTTTCCTGCGGCAACGGTAACGACCAGAAATCCGTGGAt                 >  2:714204/1‑140 (MQ=255)                                                                                                                             agCACGGCTTTCATTGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTACTGCTTCGTTTCCTGCGGCAACGGTAACGACCAGAAGTCCGTGGATTTcc             >  2:284684/1‑140 (MQ=255)                                                                                                                                aCGGCTTTCATTGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTACTGCTTCGTTTCCTGCGGCAATGGTAACGACCAGAAGTCCGTGGATTTCCCAc          <  1:898968/140‑1 (MQ=255)                                                                                                                                aCGGCTTTCATTGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTACTGCTTCGTTTCCTGCGGCAACGGTAACGACCAGAAGTCCGTGGATTTCCCAc          <  1:613879/140‑1 (MQ=255)                                                                                                                                aCGGCTTTCATTGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTACTGCTTCGTTTCCTGCGGCAACGGTAACGACCAGAAGTCCGTGGATTTCCCAc          <  1:67481/140‑1 (MQ=255)                                                                                                                                  ggCTTTCATTGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTACTGCTTCGTTTCCTGCGGCAACGGTAACGACCAGAAGTCCGTGGATTTCCCAcgc        >  1:402089/1‑140 (MQ=255)                                                                                                                                    cTTTCATTGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTACTGCTTCGTTTCCTGCGGCAACGGTAACGACCAGAAGTCCGTGGATTTCCCACgcgc      >  2:935983/1‑140 (MQ=255)                                                                                                                                     tttCATTGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTACTGCTTCGTTTCCTGCGGCAACGGTAACGACCAGAAGTCCGTGGATTTCCCACGCGCt     <  2:318692/140‑1 (MQ=255)                                                                                                                                       tCATTGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTACTGCTTCGTTTCCTGCGGCAACGGTAACGACCAGAAGTCCGTGGATTTCCCACGCGCttt   >  2:978484/1‑140 (MQ=255)                                                                                                                                        cATTGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTACTGCTTCGTTTCCTGCGGCAACGGTAACGACCAGAAGTCCGTGGATTTCCCACGCGCTTTg  <  2:508179/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                         ACTTGCGCCCAGCGTGGAGCCTTTTACGCCA‑GCGTTGAATGTCTTCAGCTTGCTGACGGAAATCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTTAGGCACGTACTGCTTCGTTTCCTGCGGCAACGGTAACGACCAGAAGTCCGTGGATTTCCCACGCGCTTTG  >  NC_000913/233056‑233329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑