Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,250,657	C→A	A314E (GCG→GAG)	yeiH →	conserved inner membrane protein YeiH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,250,657	0	C	A	100.0%	225.2 / NA	64	A314E (GCG→GAG)	yeiH	conserved inner membrane protein YeiH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (35/29);  total (35/29)

TTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAACCACTACATCTTGCTCCTGTTAACCC  >  NC_000913/2250527‑2250793                                                                                                                                   |                                                                                                                                         ttCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGAGCTGaaaaa                                                                                                                                 >  2:412429/1‑140 (MQ=255)      cGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGAGCTGAAAAAAGCTg                                                                                                                            >  1:318288/1‑140 (MQ=255)      cGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGAGCTGAAAAAAGCTg                                                                                                                            >  1:207602/1‑140 (MQ=255)                           gTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGAt                                                                                                         >  1:998894/1‑138 (MQ=255)                           gTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGAt                                                                                                         <  2:998894/138‑1 (MQ=255)                             tCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCa                                                                                                     <  2:116484/140‑1 (MQ=255)                             tCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCa                                                                                                     >  1:923735/1‑140 (MQ=255)                             tCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACACTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGAt                                                                                                         <  2:1002396/136‑1 (MQ=255)                             tCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACACTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGAt                                                                                                         >  1:1002396/1‑136 (MQ=255)                                       aCCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGt                                                                                           >  1:207626/1‑140 (MQ=255)                                       aCCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGt                                                                                           >  2:867103/1‑140 (MQ=255)                                            agagCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGATCACTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGcc                                                                                      >  2:222000/1‑140 (MQ=255)                                              agCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTg                                                                                    >  2:102066/1‑140 (MQ=255)                                               gCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGcc                                                                                      >  2:30464/1‑137 (MQ=255)                                               gCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGcc                                                                                      <  1:30464/137‑1 (MQ=255)                                               gCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTgg                                                                                   >  1:178844/1‑140 (MQ=255)                                               gCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTgg                                                                                   >  2:953151/1‑140 (MQ=255)                                                cGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGATCACTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGc                                                                                  >  1:930136/1‑140 (MQ=255)                                                cGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGc                                                                                  >  2:676683/1‑140 (MQ=255)                                                cGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGc                                                                                  >  1:162278/1‑140 (MQ=255)                                                       aaCATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGAttgt                                                                           <  2:321829/140‑1 (MQ=255)                                                            gCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTtggtg                                                                      >  1:293822/1‑140 (MQ=255)                                                             cTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTg                                                                                <  1:727478/129‑1 (MQ=255)                                                             cTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTg                                                                                >  2:727478/1‑129 (MQ=255)                                                               ggTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTtggtggtg                                                                   >  1:312089/1‑140 (MQ=255)                                                                 tAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGAttgtt                                                                          >  1:836451/1‑131 (MQ=255)                                                                 tAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGAttgtt                                                                          <  2:836451/131‑1 (MQ=255)                                                                 tAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTggtggtggt                                                                 <  2:707215/140‑1 (MQ=255)                                                                    cGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGATCACTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCg                                                              <  2:930136/140‑1 (MQ=255)                                                                    cGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCg                                                              <  1:1053475/140‑1 (MQ=255)                                                                    cGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCg                                                              <  2:207602/140‑1 (MQ=255)                                                                    cGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCg                                                              <  1:869352/140‑1 (MQ=255)                                                                      cTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGAt                                                            <  1:43991/140‑1 (MQ=255)                                                                      cTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGAt                                                            >  1:539113/1‑140 (MQ=255)                                                                          aTACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAAc                                                        >  1:872343/1‑140 (MQ=255)                                                                           tACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACt                                                       <  2:666731/140‑1 (MQ=255)                                                                              cTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTAtg                                                    >  1:905175/1‑140 (MQ=255)                                                                                   tgctgGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTaa                                                         <  1:23501/130‑1 (MQ=255)                                                                                   tgctgGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTaa                                                         >  2:23501/1‑130 (MQ=255)                                                                                    gctgGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACt                                                       >  1:937898/1‑131 (MQ=255)                                                                                    gctgGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACt                                                       <  2:937898/131‑1 (MQ=255)                                                                                     ctgGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGACTCa                                             >  2:978926/1‑140 (MQ=255)                                                                                           atggcgatggcgGCGCTGGGTCTGACCACTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGAtt                                               >  2:837989/1‑132 (MQ=255)                                                                                           atggcgatggcgGCGCTGGGTCTGACCACTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGAtt                                               <  1:837989/132‑1 (MQ=255)                                                                                            tggcgatggcgGCGCTGGGTCTGACCACTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTc                                      <  1:412429/140‑1 (MQ=255)                                                                                             ggcgatggcgGCGCTGGGTCTGACCACTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCa                                     <  2:312089/140‑1 (MQ=255)                                                                                             ggcgatggcgGCGCTGGGTCTGACCACTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGACTCAAAGCGTCa                                     <  1:978926/140‑1 (MQ=255)                                                                                               cgatggcgGCGCTGGGTCTGACCACTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATc                                   >  2:173136/1‑140 (MQ=255)                                                                                                  tggcgGCGCTGGGTCTGACCACTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCa                                >  2:1005349/1‑140 (MQ=255)                                                                                                  tggcgGCGCTGGGTCTGACCACTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCa                                >  1:828686/1‑140 (MQ=255)                                                                                                    gcggcgCTGGGTCTGACCACTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATa                              <  1:173136/140‑1 (MQ=255)                                                                                                    gcggcgCTGGGTCTGACCACTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATa                              >  1:482762/1‑140 (MQ=255)                                                                                                        cgcTGGGTCTGACCACTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAAc                           <  2:323903/139‑1 (MQ=255)                                                                                                        cgcTGGGTCTGACCACTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAAc                           >  1:323903/1‑139 (MQ=255)                                                                                                                 tGACCCCTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAACCACTACATCt                 <  2:716432/140‑1 (MQ=255)                                                                                                                   aCCACTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAACCACTACATCTTg               <  2:51874/140‑1 (MQ=255)                                                                                                                    ccACTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAACCACTACATCTTGc              <  1:953151/140‑1 (MQ=255)                                                                                                                       cTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAACCACTACATCTTGc              <  2:698788/137‑1 (MQ=255)                                                                                                                       cTCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAACCACTACATCTTGc              >  1:698788/1‑137 (MQ=255)                                                                                                                        tCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAACCACTACATCTTGc              <  2:105035/136‑1 (MQ=255)                                                                                                                        tCACGTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAACCACTACATCTTGc              >  1:105035/1‑136 (MQ=255)                                                                                                                            gTCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAACCACTACATCTTGCTCCTGTTa      <  2:773399/140‑1 (MQ=255)                                                                                                                             tCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAACCACTACATCTTGc              <  2:228364/131‑1 (MQ=255)                                                                                                                             tCAGCGAGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAACCACTACATCTTGc              >  1:228364/1‑131 (MQ=255)                                                                                                                                gcgagcTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAACCACTACATCTTGCTCCTGTTAAccc  <  2:318288/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                         TTCATCGTAGTTGCCATCTTTAACTCGTTCCACCTGTTACCGCAGAGCGTGGTGAACATGCTGGTAACGCTGGATACCTTCCTGCTGGCAATGGCGATGGCGGCGCTGGGTCTGACCACTCACGTCAGCGCGCTGAAAAAAGCTGGGGCGAAACCTCTGCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAACCACTACATCTTGCTCCTGTTAACCC  >  NC_000913/2250527‑2250793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑