Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,294,874	A→G	C11R (TGC→CGC)	ccmF ←	holocytochrome c synthetase membrane subunit CcmF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,294,874	0	A	G	100.0%	184.9 / NA	50	C11R (TGC→CGC)	ccmF	holocytochrome c synthetase membrane subunit CcmF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/27);  total (23/27)

ACCAGAAATGCGCCAGCCACAGACATAAACAGCAGCCAGGCAAACAAGCGGGAAGACGCCATCATGCGCGCATCTCCGCGCGCCACGCCCCATAGCGGATACACGGACAGCAGCAGCGCAATTCCCAGCGCCAGGCACAGCAGTCCGTTACCAATTTCTGGCATCATGATGCTGGGTCCTTATAAACACTCGCCGGGCGACGGTGGTTAGCTTCCATCGCTTTCTCAACTTCTGGCGGCGTATAGTTTTCATCGTGTTTCGCCAGCACTTCTT  >  NC_000913/2294738‑2295010                                                                                                                                         |                                                                                                                                         aCCAGAAATGCGCCAGCCACAGACATAAACAGCAGCCAGGCAAACAAGCGGGAAGACGCCATCATGCGCGCATCTCCGCGCGCCACGCCCCATAGCGGATACACGGACAGCAGCAGCGCAATTCCCAGCGCCAGGCGcag                                                                                                                                       <  2:419848/140‑1 (MQ=255) aCCAGAAATGCGCCAGCCACAGACATAAACAGCAGCCAGGCAAACAAGCGGGAAGACGCCATCATGCGCGCATCTCCGCGCGCCACGCCCCATAGCGGATACACGGACAGCAGCAGCGCAATTCCCAGCGCCAGGCGcag                                                                                                                                       <  1:326868/140‑1 (MQ=255)   cAGAAATGCGCCAGCCACAGACATAAACAGCAGCCAGGCAAACAAGCGGGAAGACGCCATCATGCGCGCATCTCCGCGCGCCACGCCCCATAGCGGATACACGGACAGCAGCAGCGCAATTCCCAGCGCCAGGCgcagca                                                                                                                                     <  1:431464/140‑1 (MQ=255)   cAGAAATGCGCCAGCCACAGACATAAACAGCAGCCAGGCAAACAAGCGGGAAGACGCCATCATGCGCGCATCTCCGCGCGCCACGCCCCATAGCGGATACACGGACAGCAGCAGCGCAATTCCCAGCGCCAGGCgcagca                                                                                                                                     <  1:401580/140‑1 (MQ=255)     gAAATGCGCCAGCCACAGACATAAACAGCAGCCAGGCAAACAAGCGGGAAGACGCCATCATGCGCGCATCTCCGCGCGCCACGCCCCATAGCGGATACACGGACAGCAGCAGCGCAATTCCCAGCGCCAGGCGCAGCAGt                                                                                                                                   >  2:294330/1‑140 (MQ=255)            gccagccaCAGACATAAACAGCAGCCAGGCAAACAAGCGGGAAGACGCCATCATGCGCGCATCTCCGCGCGCCACGCCCCATAGCGGATACACGGACAGCAGCAGCGCAATTCCCAGCGCCAGGCGCAGCAGTCCGTTAc                                                                                                                            <  1:599882/140‑1 (MQ=255)                        cATAAACAGCAGCCAGGCAAACAAGCGGGAAGACGCCATCATGCGCGCATCTCCGCGCGCCACGCCCCATAGCGGATACACGGACAGCAGCAGCGCAATTCCCAGCGCCAGGCGCAGCAGTCCGTTACCAATTTCTGGca                                                                                                                <  1:118828/140‑1 (MQ=255)                            aaCAGCAGCCAGGCAAACAAGCGGGAAGACGCCATCATGCGCGCATCTCCGCGCGCCACGCCCCATAGCGGATACACGGACAGCAGCAGCGCAATTCCCAGCGCCAGGCGCAGCAGTCCGTTACCAATTTCTGGcatcat                                                                                                            >  2:341235/1‑140 (MQ=255)                                gcagcCAGGCAAACAAGCGGGAAGACGCCATCATGCGCGCATCTCCGCGCGCCACGCCCCATAGCGGATACACGGACAGCAGCAGCGCAATTCCCAGCGCCAGGCGCAGCAGTCCGTTACCAATTTCTGGCATCatgatg                                                                                                        <  1:366418/140‑1 (MQ=255)                                  agcCAGGCAAACAAGCGGGAAGACGCCATCATGCGCGCATCTCCGCGCGCCACGCCCCATAGCGGATACACGGACAGCAGCAGCGCAATTCCCAGCGCCAGGCGCAGCAGTCCGTTACCAATTTCTGGCATCATGATGCt                                                                                                      <  1:991639/140‑1 (MQ=255)                                   gcCAGGCAAACAAGCGGGAAGACGCCATCATGCGCGCATCTCCGCGCGCCACGCCCCATAGCGGATACACGGACAGCAGCAGCGCAATTCCCAGCGCCAGGCGCAGCAGTCCGTTACCAATTTCTGGCATCATGATGCTg                                                                                                     >  2:83620/1‑140 (MQ=255)                                   gcCAGGCAAACAAGCGGGAAGACGCCATCATGCGCGCATCTCCGCGCGCCACGCCCCATAGCGGATACACGGACAGCAGCAGCGCAATTCCCAGCGCCAGGCGCAGCAGTCCGTTACCAATTTCTGGCATCATGATGCTg                                                                                                     >  2:669470/1‑140 (MQ=255)                                    ccAGGCAAACAAGCGGGAAGACGCCATCATGCGCGCATCTCCGCGCGCCACGCCCCATAGCGGATACACGGACAGCAGCAGCGCAATTCCCAGCGCCAGGCGCAGCAGTCCGTTACCAATTTCTGGCATCATGATGCTgg                                                                                                    >  2:26149/1‑140 (MQ=255)                                    ccAGGCAAACAAGCGGGAAGACGCCATCATGCGCGCATCTCCGCGCGCCACGCCCCATAGCGGATACACGGACAGCAGCAGCGCAATTCCCAGCGCCAGGCGCAGCAGTCCGTTACCAATTTCTGGCATCATGATGCTgg                                                                                                    <  2:162137/140‑1 (MQ=255)                                     cAGGCAAACAAGCGGGAAGACGCCATCATGCGCGCATCTCCGCGCGCCACGCCCCATAGCGGATACACGGACAGCAGCAGCGCAATTCCCAGCGCCAGGCGCAGCAGTCCGTTACCAATTTCTGGCATCATGATGCTggg                                                                                                   <  2:557268/140‑1 (MQ=255)                                     cAGGCAAACAAGCGGGAAGACGCCATCATGCGCGCATCTCCGCGCGCCACGCCCCATAGCGGATACACGGACAGCAGCAGCGCAATTCCCAGCGCCAGGCGCAGCAGTCCGTTACCAATTTCTGGCATCATGATGCTggg                                                                                                   <  1:1784/140‑1 (MQ=255)                                     cAGGCAAACAAGCGGGAAGACGCCATCATGCGCGCATCTCCGCGCGCCACGCCCCATAGCGGATACACGGACAGCAGCAGCGCAATTCCCAGCGCCAGGCGCAGCAGTCCGTTACCAATTTCTGGCATCATGATGCTggg                                                                                                   <  1:238655/140‑1 (MQ=255)                                     cAGGCAAACAAGCGGGAAGACGCCATCATGCGCGCATCTCCGCGCGCCACGCCCCATAGCGGATACACGGACAGCAGCAGCGCAATTCCCAGCGCCAGGCGCAGCAGTCCGTTACCAATTTCTGGCATCATGATGCTggg                                                                                                   <  2:769353/140‑1 (MQ=255)                                      aGGCAAACAAGCGGGAAGACGCCATCATGCGCGCATCTCCGCGCGCCACGCCCCATAGCGGATACACGGACAGCAGCAGCGCAATTCCCAGCGCCAGGCGCAGCAGTCCGTTACCAATTTCTGGCATCATGATGCTGGGt                                                                                                  <  1:669721/140‑1 (MQ=255)                                       ggCAAACAAGCGGGAAGACGCCATCATGCGCGCATCTCCGCGCGCCACGCCCCATAGCGGATACACGGACAGCAGCAGCGCAATTCCCAGCGCCAGGCGCAGCAGTCCGTTACCAATTTCTGGCATCATGATGCTGGGTc                                                                                                 <  1:294330/140‑1 (MQ=255)                                       ggCAAACAAGCGGGAAGACGCCATCATGCGCGCATCTCCGCGCGCCACGCCCCATAGCGGATACACGGACAGCAGCAGCGCAATTCCCAGCGCCAGGCGCAGCAGTCCGTTACCAATTTCTGGCATCATGATGCTGGGTc                                                                                                 <  1:26149/140‑1 (MQ=255)                                         cAAACAAGCGGGAAGACGCCATCATGCGCGCATCTCCGCGCGCCACGCCCCATAGCGGATACACGGACAGCAGCAGCGCAATTCCCAGCGCCAGGCGCAGCAGTCCGTTACCAATTTCTGGCATCATGATGCTGGGTCCt                                                                                               >  2:298434/1‑140 (MQ=255)                                                          gCCATCATGCGCGCATCTCCGCGCGCCACGCCCCATAGCGGATACACGGACAGCAGCAGCGCAATTCCCAGCGCCAGGCGCAGCAGTCCGTTACCAATTTCTGGCATCATGATGCTGGGTCCTTATAAACACTCGCCggg                                                                              <  2:502615/140‑1 (MQ=255)                                                          gCCATCATGCGCGCATCTCCGCGCGCCACGCCCCATAGCGGATACACGGACAGCAGCAGCGCAATTCCCAGCGCCAGGCGCAGCAGTCCGTTACCAATTTCTGGCATCATGATGCTGGGTCCTTATAAACACTCGCCggg                                                                              <  2:676922/140‑1 (MQ=255)                                                               catGCGCGCATCTCCGCGCGCCACGCCCCATAGCGGATACACGGACAGCAGCAGCGCAATTCCCAGCGCCAGGCGCAGCAGTCCGTTACCAATTTCTGGCATCATGATGCTGGGTCCTTATAAACACTCGCCGGGCGACg                                                                         >  2:360622/1‑140 (MQ=255)                                                                  gcgcgcATCTCCGCGCGCCACGCCTCATAGCGGATACACGGACAGCAGCAGCGCAATTCCCAGCGCCAGGCGCAGCAGTCCGTTACCAATTTCTGGCATCATGATGCTGGGTCCTTATAAACACTCGCCGGGCGACggtg                                                                      >  2:675272/1‑140 (MQ=255)                                                                   cgcgcATCTCCGCGCGCCACGCCCCATAGCGGATACACGGACAGCAGCAGCGCAATTCCCAGCGCCAGGCGCAGCAGTCCGTTACCAATTTCTGGCATCATGATGCTGGGTCCTTATAAACACTCGCCGGGCGACggtgg                                                                     >  2:803125/1‑140 (MQ=255)                                                                   cgcgcATCGCCGCGCGCCACGCCCCATAGCGGATACACGGACAGCAGCAGCGGAATTCCCAGCGCCAGGCGCAGCAGTCCGTTACCAATTTCTGGCATCATGATGCTGGGTCCTTATAAACACTCGCCGGGCGACggtgg                                                                     <  2:585292/140‑1 (MQ=255)                                                                     cgcATCTCCGCGCGCCATGCCCCATAGCGGATACACGGACAGCAGCAGCGCAATTCCCAGCGCCAGGCGCAGCAGTCCGTTACCAATTTCTGGCATCATGATGCTGGGTCCTTATAAACACTCGCCGGGCGACGGTGGtt                                                                   >  1:360623/1‑140 (MQ=255)                                                                        aTCTCCGCGCGCCACGCCCCATAGCGGATACACGGACAGCAGCAGCGCAATTCCCAGCGCCAGGCGCAGCAGTCCGTTACCAATTTCTGGCATCATGATGCTGGGTCCTTATAAACACTCGCCGGGCGACGGTGGTTAGc                                                                >  1:13020/1‑140 (MQ=255)                                                                             cgcgcgCCACGCCCCATAGCGGATACACGGACAGCAGCAGCGCAATTCCCAGCGCCAGGCGCAGCAGTCCGTTACCAATTTCTGGCATCATGATGCTGGGTCCTTATAAACACTCGCCGGGCGACGGTGGTTAGCTTCCa                                                           >  1:675480/1‑140 (MQ=255)                                                                                gcgcCACGCCCCATAGCGGATACACGGACAGCAGCAGCGCAATTCCCAGCGCCAGGCGCAGCAGTCCGTTACCAATTTCTGGCATCATGATGCTGGGTCCTTATAAACACTCGCCGGGCGACGGTGGTTAGCTTCCATCg                                                        <  1:341235/140‑1 (MQ=255)                                                                                 cgcCACGCCCCATAGCGGATACACGGACAGCAGCAGCGCAATTCCCAGCGCCAGGCGCAGCAGTCCGTTACCAATTTCTGGCATCATGATGCTGGGTCCTTATAAACACTCGCCGGGCGACGGTGGTTAGCTTCCATCGc                                                       <  1:298434/140‑1 (MQ=255)                                                                                              aGCGGATACACGGACAGCAGCAGCGCAATTCCCAGCGCCAGGCGCAGCAGTCCGTTACCAATTTCTGGCATCATGATGCTGGGTCCTTATAAACACTCGCCGGGCGACGGTGGTTAGCTTCCATCGCTTTCTCAACTTCt                                          >  2:536296/1‑140 (MQ=255)                                                                                               gCGGATACACGGACAGCAGCAGCGCAATTCCCAGCGCCAGGCGCAGCAGTCCGTTACCAATTTCTGGCATCATGATGCTGGGTCCTTATAAACACTCGCCGGGCGACGGTGGTTAGCTTCCATCGCTTTCTCAACTTCTg                                         <  2:13020/140‑1 (MQ=255)                                                                                                  gATACACGGACAGCAGCAGCGCAATTCCCAGCGCCAGGCGCAGCAGTCCGTTACCAATTTCTGGCATCATGATGCTGGGTCCTTATAAACACTCGCCGGGCGACGGTGGTTAGCTTCCATCGCTTTCTCAACTTCTggcg                                      <  1:803125/140‑1 (MQ=255)                                                                                                   aTACACGGACAGCAGCAGCGCAATTCCCAGCGCCAGGCGCAGCAGTCCGTTACCAATTTCTGGCATCATGATGCTGGGTCCTTATAAACACTCGCCGGGCGACGGTGGTTAGCTTCCATCGCTTTCTCAACTTCTggcgg                                     >  1:259200/1‑140 (MQ=255)                                                                                                   aTACACGGACAGCAGCAGCGCAATTCCCAGCGCCAGGCGCAGCAGTCCGTTACCAATTTCTGGCATCATGATGCTGGGTCCTTATAAACACTCGCCGGGCGACGGTGGTTAGCTTCCATCGCTTTCTCAACTTCTggcg                                      <  1:474004/139‑1 (MQ=255)                                                                                                   aTACACGGACAGCAGCAGCGCAATTCCCAGCGCCAGGCGCAGCAGTCCGTTACCAATTTCTGGCATCATGATGCTGGGTCCTTATAAACACTCGCCGGGCGACGGTGGTTAGCTTCCATCGCTTTCTCAACTTCTggcg                                      >  2:474004/1‑139 (MQ=255)                                                                                                    tACACGGACAGCAGCAGCGCAATTCCCAGCGCCAGGCGCAGCAGTCCGTTACCAATTTCTGGCATCATGATGCTGGGTCCTTATAAACACTCGCCGGGCGACGGTGGTTAGCTTCCATCGCTTTCTCAACTTCTggcggc                                    <  2:169047/140‑1 (MQ=255)                                                                                                      cacGGACAGCAGCAGCGCGATTCCCAGCGCCAGGCGCAGCAGTCCGTTACCAATTTCTGGCATCATGATGCTGGGTCCTTATAAACACTCGCCGGGCGACGGTGGTTAGCTTCCATCGCTTTCTCAACTTCTGGCGGCGt                                  <  2:476538/140‑1 (MQ=255)                                                                                                        cGGACAGCAGCAGCGCAATTCCCAGCGCCAGGCGCAGCAGTCCGTTACCAATTTCTGGCATCATGATGCTGGGTCCTTATAAACACTCGCCGGGCGACGGTGGTTAGCTTCCATCGCTTTCTCAACTTCTGGCGGCGtat                                <  2:675480/140‑1 (MQ=255)                                                                                                        cGGACAGCAGCAGCGCAATTCCCAGCGCCAGGCGCAGCAGTCCGTTACCAATTTCTGGCATCATGATGCTGGGTCCTTATAAACACTCGCCGGGCGACGGTGGTTAGCTTCCATCGCTTTCTCAACTTCTGGCGGCGtat                                <  1:669470/140‑1 (MQ=255)                                                                                                         ggACAGCAGCAGCGCAATTCCCAGCGCCAGGCGCAGCAGTCCGTTACCAATTTCTGGCATCATGATGCTGGGTCCTTATAAACACTCGCCGGGCGACGGTGGTTAGCTTCCATCGCTTTCTCAACTTCTGGCGGCGtata                               >  2:337693/1‑140 (MQ=255)                                                                                                             agcagcagcGCAATTCCCAGCGCCAGGCGCAGCAGTCCGTTACCAATTTCTGGCATCATGATGCTGGGTCCTTATAAACACTCGCCGGGCGACGGTGGTTAGCTTCCATCGCTTTCTCAACTTCTGGCGGCGTATAGttt                           >  2:777900/1‑140 (MQ=255)                                                                                                               cagcagcGCAATTCCCAGCGCCAGGCGCAGCAGTCCGTTACCAATTTCTGGCATCATGATGCTGGGTCCTTATAAACACTCGCCGGGCGACGGTGGTTAGCTTCCATCGCTTTCTCAACTTCTGGCGGCGTATAGTTTTc                         >  2:147442/1‑140 (MQ=255)                                                                                                                     cgcAATTCCCAGCGCCAGGCGCAGCAGTCCGTTACCAATTTCTGGCATCATGATGCTGGGTCCTTATAAACACTCGCCGGGCGACGGTGGTTAGCTTCCATCGCTTTCTCAACTTCTGGCGGCGTATAGTTTTCATCgtg                   >  1:193139/1‑140 (MQ=255)                                                                                                                     cgcAATTCCCAGCGCCAGGCGCAGCAGTCCGTTACCAATTTCTGGCATCATGATGCTGGGTCCTTATAAACACTCGCCGGGCGACGGTGGTTAGCTTCCATCGCTTTCTCAACTTCTGGCGGCGTATAGTTTTCATCgtg                   >  1:550915/1‑140 (MQ=255)                                                                                                                     cgcAATTCCCAGCGCCAGGCGCAGCAGTCCGTTACCAATTTCTGGCATCATGATGCTGGGTCCTTATAAACACTCGCCGGGCGACGGTGGTTAGCTTCCATCGCTTTCTCAACTTCTGGCGGCGTATAGTTTTCATCgtg                   >  2:50044/1‑140 (MQ=255)                                                                                                                           tCCCAGCGCCAGGCGCAGCAGTCCGTTACCAATTTCTGGCATCATGATGCTGGGTCCTTATAAACACTCGCCGGGCGACGGTGGTTAGCTTCCATCGCTTTCTCAACTTCTGGCGGCGTATAGTTTTCATCGTGTTTCGc             <  1:360622/140‑1 (MQ=255)                                                                                                                                    cAGGCGCAGCAGTCCGTTACCAATTTCTGGCATCATGATGCTGGGTCCTTATAAACACTCGCCGGGCGACGGTGGTTAGCTTCCATCGCTTTCTCAACTTCTGGCGGCGTATAGTTTTCATCGTGTTTCGCCAGCActtc    >  2:586322/1‑140 (MQ=255)                                                                                                                                      ggCGCAGCAGTCCGTTACCAATTTCTGGCATCATGATGCTGGGTCCTTATAAACACTCGCCGGGCGACGGTGGTTAGCTTCCATCGCTTTCTCAACTTCTGGCGGCGTATAGTTTTCATCGTGTTTCGCCAGCActtctt  >  1:532296/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                         ACCAGAAATGCGCCAGCCACAGACATAAACAGCAGCCAGGCAAACAAGCGGGAAGACGCCATCATGCGCGCATCTCCGCGCGCCACGCCCCATAGCGGATACACGGACAGCAGCAGCGCAATTCCCAGCGCCAGGCACAGCAGTCCGTTACCAATTTCTGGCATCATGATGCTGGGTCCTTATAAACACTCGCCGGGCGACGGTGGTTAGCTTCCATCGCTTTCTCAACTTCTGGCGGCGTATAGTTTTCATCGTGTTTCGCCAGCACTTCTT  >  NC_000913/2294738‑2295010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑