Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,408,070	T→C	R170R (CGT→CGC)	alaA →	glutamate‑‑pyruvate aminotransferase AlaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,408,070	0	T	C	100.0%	158.5 / NA	43	R170R (CGT→CGC)	alaA	glutamate‑‑pyruvate aminotransferase AlaA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/27);  total (16/27)

CTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGTGGGATCGTTATTATCAACCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAATGGAGATTGTGGAGATTGCACGTCAGCATAATCTCATTATCTTCGCCGATGAAATTTATGACAAAATTCTCT  >  NC_000913/2407934‑2408203                                                                                                                                         |                                                                                                                                      cTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCggg                                                                                                                                    <  1:812864/140‑1 (MQ=255) cTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCggg                                                                                                                                    <  1:520293/140‑1 (MQ=255)  tGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGa                                                                                                                                   <  1:624439/140‑1 (MQ=255)  tGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGa                                                                                                                                   <  2:795526/140‑1 (MQ=255)  tGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGa                                                                                                                                   >  1:451188/1‑140 (MQ=255)  tGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGa                                                                                                                                   <  1:598247/140‑1 (MQ=255)       cAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGtt                                                                                                                              <  1:989974/140‑1 (MQ=255)        aGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGtta                                                                                                                             >  1:605572/1‑140 (MQ=255)            tACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTtattat                                                                                                                         <  2:605572/140‑1 (MQ=255)              cccACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCa                                                                                                                       <  1:602404/140‑1 (MQ=255)               ccACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCaa                                                                                                                      <  2:529311/140‑1 (MQ=255)                   tctGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCa                                                                                                                  >  2:483370/1‑140 (MQ=255)                    ctGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTtattat                                                                                                                         <  1:542533/132‑1 (MQ=255)                    ctGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTtattat                                                                                                                         >  2:542533/1‑132 (MQ=255)                        aCCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCAAATaa                                                                                                             >  1:692847/1‑140 (MQ=255)                        aCCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCAAATaa                                                                                                             >  1:551451/1‑140 (MQ=255)                            cggcggTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCAAATAACCCa                                                                                                         >  1:749376/1‑140 (MQ=255)                            cggcggTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCAAATAACCCa                                                                                                         >  2:212014/1‑140 (MQ=255)                              gcggTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCAAATAACCCAAc                                                                                                       <  1:212014/140‑1 (MQ=255)                                  tttCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCAAATAACCCAACCGgc                                                                                                   <  1:178138/140‑1 (MQ=255)                                   ttCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCAAATAACCCAACCGgcg                                                                                                  <  1:592320/140‑1 (MQ=255)                                   ttCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCAAATAACCCAACCGgcg                                                                                                  <  2:749376/140‑1 (MQ=255)                                   ttCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCAAATAACCCAACCGgcg                                                                                                  <  1:818085/140‑1 (MQ=255)                                   ttCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCAAATAACCCAACCGgcg                                                                                                  <  1:483370/140‑1 (MQ=255)                                     cGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCAAATAACCCAACCGGcgcg                                                                                                <  1:752046/140‑1 (MQ=255)                                                    aaGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCAAATAACCCAACCGgcg                                                                                                  <  1:651507/123‑1 (MQ=255)                                                    aaGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCAAATAACCCAACCGgcg                                                                                                  >  2:651507/1‑123 (MQ=255)                                                    aaGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCAAATAACCCAACCGGCGCGGTATATTCCAAagag                                                                                 >  2:287531/1‑140 (MQ=255)                                                              ttATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAAtgg                                                                       <  1:527728/140‑1 (MQ=255)                                                              ttATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAAtgg                                                                       <  1:444986/140‑1 (MQ=255)                                                                      gCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAAtggagattgtg                                                               <  2:1013206/140‑1 (MQ=255)                                                                        gATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCACTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAAtggagattgtgga                                                             <  2:988845/140‑1 (MQ=255)                                                                             aTCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAAtggagattgtggagattg                                                        <  1:724054/140‑1 (MQ=255)                                                                             aTCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAAtggagattgtggagattg                                                        <  1:369678/140‑1 (MQ=255)                                                                                  ctGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAATGGAGATTGTGGAGATTGCACGt                                                   <  2:551451/140‑1 (MQ=255)                                                                                    gACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAATGGAGATTGTGGAGATTGCACGTCa                                                 >  2:269120/1‑140 (MQ=255)                                                                                     aCTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAATGGAGATTGTGGAGATTGCACGTCaa                                                <  1:269120/140‑2 (MQ=255)                                                                                         gTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAATGGAGATTGTGGAGATTGCACGTCAGCATa                                            >  1:587757/1‑140 (MQ=255)                                                                                         gTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAATGGAGATTGTGGAGATTGCACGTCAGCATa                                            >  1:483901/1‑140 (MQ=255)                                                                                                           tatTCGCGCTAAAATTACGCCTCGTACGCGCGGGATCGTTATTATCAACCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAATGGAGATTGTGGAGATTGCACGTCAGCATAATCTCATTATCTTCGCCg                          >  1:724979/1‑140 (MQ=255)                                                                                                                                 cGTACGCGCGGGATCGTTATTATCAACCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAATGGAGATTGTGGAGATTGCACGTCAGCATAATCTCATTATCTTCGCCGATGAAATTTATGACa           <  1:964465/133‑1 (MQ=255)                                                                                                                                 cGTACGCGCGGGATCGTTATTATCAACCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAATGGAGATTGTGGAGATTGCACGTCAGCATAATCTCATTATCTTCGCCGATGAAATTTATGACa           >  2:964465/1‑133 (MQ=255)                                                                                                                                   tACGCGCGGGATCGTTATTATCAACCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAATGGAGATTGTGGAGATTGCACGTCAGCATAATCTCATTATCTTCGCCGATGAAATTTATGACAAAATTctct  >  2:932942/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      CTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAATTACGCCTCGTACGCGTGGGATCGTTATTATCAACCCAAATAACCCAACCGGCGCGGTATATTCCAAAGAGCTTTTAATGGAGATTGTGGAGATTGCACGTCAGCATAATCTCATTATCTTCGCCGATGAAATTTATGACAAAATTCTCT  >  NC_000913/2407934‑2408203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑