Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,549,919	T→C	E209E (GAA→GAG)	yfeX ←	porphyrinogen peroxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,549,919	0	T	C	98.4%	217.4 / ‑4.3	61	E209E (GAA→GAG)	yfeX	porphyrinogen peroxidase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (29/31);  total (30/31)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.24e-01

CGCAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAACGCTGGACAAACACATAGCTGCCGCCCGC  >  NC_000913/2549789‑2550050                                                                                                                                   |                                                                                                                                    cgcAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCg                                                                                                                            >  1:140015/1‑140 (MQ=255)      tAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGAt                                                                                                                       >  2:606085/1‑140 (MQ=255)      tAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGAt                                                                                                                       >  1:518632/1‑140 (MQ=255)        ggCGCAGAAGTACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGAtct                                                                                                                     <  2:4629/140‑1 (MQ=255)             aGAAGTACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCg                                                                                                                <  2:715226/140‑1 (MQ=255)              gAAGTACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCGt                                                                                                               >  1:818941/1‑140 (MQ=255)               aaGTACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCGtt                                                                                                              <  2:457711/140‑1 (MQ=255)               aaGTACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCGtt                                                                                                              <  1:956195/140‑1 (MQ=255)               aaGTACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCGtt                                                                                                              <  2:815436/140‑1 (MQ=255)                aGTACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCGTTg                                                                                                             >  2:813099/1‑140 (MQ=255)                 gTACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCGTTgg                                                                                                            <  1:887422/140‑1 (MQ=255)                           tGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGt                                                                                                  >  2:1045596/1‑140 (MQ=255)                           tGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGt                                                                                                  >  2:80717/1‑140 (MQ=255)                            gAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAATGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTg                                                                                                 <  2:986137/140‑1 (MQ=255)                            gAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTg                                                                                                 >  1:721532/1‑140 (MQ=255)                                 ccACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGccc                                                                                            >  1:748111/1‑140 (MQ=255)                                   aCTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGa                                                                                          <  1:640612/140‑1 (MQ=255)                                   aCTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGa                                                                                          >  2:195948/1‑140 (MQ=255)                                   aCTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGa                                                                                          <  1:1045596/140‑1 (MQ=255)                                        cAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCAcc                                                                                     <  1:80717/140‑1 (MQ=255)                                        cAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGGGCGCCCGATCAcc                                                                                     >  2:317642/1‑140 (MQ=255)                                           tGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATc                                                                                        <  1:441057/134‑1 (MQ=255)                                           tGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATc                                                                                        >  2:441057/1‑134 (MQ=255)                                             ccGTACGGTAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCAtctc                                                                                >  1:211046/1‑140 (MQ=255)                                             ccGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCAtctc                                                                                <  1:606085/140‑1 (MQ=255)                                                tACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCAcc                                                                                     <  1:568800/132‑1 (MQ=255)                                                tACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCAcc                                                                                     >  2:568800/1‑132 (MQ=255)                                                tACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTg                                                                             >  1:670510/1‑140 (MQ=255)                                                tACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTg                                                                             >  2:25988/1‑140 (MQ=255)                                                     cAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCAtc                                                                                  >  2:374788/1‑130 (MQ=255)                                                     cAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCAtc                                                                                  <  1:374788/130‑1 (MQ=255)                                                           ctGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGc                                                                  <  2:756434/140‑1 (MQ=255)                                                              gCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCa                                                               >  2:165083/1‑140 (MQ=255)                                                                        ttCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAg                                                     <  2:818941/140‑1 (MQ=255)                                                                          cAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAgct                                                   <  2:413294/140‑1 (MQ=255)                                                                           aGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAgctg                                                  >  1:703864/1‑140 (MQ=255)                                                                             cccTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAg                                                     >  1:699803/1‑135 (MQ=255)                                                                             cccTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAg                                                     <  2:699803/135‑1 (MQ=255)                                                                                tttGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCa                                             >  2:125759/1‑140 (MQ=255)                                                                                  tGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAg                                                     <  2:1005632/130‑1 (MQ=255)                                                                                  tGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAg                                                     >  1:1005632/1‑130 (MQ=255)                                                                                      aTCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTtgt                                       <  2:748111/140‑1 (MQ=255)                                                                                       tcttctTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTtgtg                                      >  1:114132/1‑140 (MQ=255)                                                                                           cttTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTcc                                  <  2:114132/140‑1 (MQ=255)                                                                                           cttTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTcc                                  <  2:518632/140‑1 (MQ=255)                                                                                                aGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAACg                             <  1:317642/140‑1 (MQ=255)                                                                                                 gATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAACGc                            <  1:195948/140‑1 (MQ=255)                                                                                                  aTCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAACGCt                           >  1:554942/1‑140 (MQ=255)                                                                                                  aTCAACGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGATCTGCTTCAGGTTGTGTTCCCAACGCt                           >  1:743306/1‑140 (MQ=255)                                                                                                  aTCAACGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAACGc                            <  1:703830/139‑1 (MQ=255)                                                                                                  aTCAACGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAACGc                            >  2:703830/1‑139 (MQ=255)                                                                                                   tCAACGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTcc                                  <  1:759764/132‑1 (MQ=255)                                                                                                   tCAACGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTcc                                  >  2:759764/1‑132 (MQ=255)                                                                                                     aaCGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCGGCTTCAGGTTGTGTTCCCAACGCTGGa                        >  1:1040308/1‑140 (MQ=255)                                                                                                      aCGCGGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAACGCTGGAc                       <  1:165083/140‑1 (MQ=255)                                                                                                       cgcgGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAACGCTGGACa                      <  2:703864/140‑1 (MQ=255)                                                                                                       cgcgGGTGAGGTGAGAGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAACGCTGGACa                      <  2:140015/140‑1 (MQ=255)                                                                                                                   gagaGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAACGCTGGACAAACACATAGCTg          <  2:721532/140‑1 (MQ=255)                                                                                                                   gagaGGTTTCCGGACGCTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAACGCTGGACAAACACATAGCTg          <  1:125759/140‑1 (MQ=255)                                                                                                                           tCCGGACGCTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAACGCTGGACAAACACATAGCTGccgcccgc  >  1:435282/1‑140 (MQ=255)                                                                                                                           tCCGGACGCTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAACGCTGGACAAACACATAGCTGccgcccgc  >  2:925851/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                    CGCAGTAGGCGCAGAAGTACAGACCGTGAGTGCCACTGGCAGTGCCGTACGGCAGGCTCTGGCGAACAATCTTCAGCCCTTTGCCATCTTCTTTCAGATCAACGCGGGTGAGGTGAGAGGTTTCCGGACGTTCGTCGCCGTCGATCTCTTCGTTGGCCTCTTTGGTGCGCCCGATCACCATCTCCTGATCGTGAACGCTCATCCGGTTGAGCTGCTTCAGGTTGTGTTCCCAACGCTGGACAAACACATAGCTGCCGCCCGC  >  NC_000913/2549789‑2550050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑