Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,573,760	G→A	L182L (CTC→CTT)	eutT ←	putative ethanolamine utilization cobalamin adenosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,573,760	0	G	A	100.0%	105.4 / NA	30	L182L (CTC→CTT)	eutT	putative ethanolamine utilization cobalamin adenosyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/19);  total (11/19)

GCGTAATACTTCAAAGCTGCGGGTGATAAAGACCTGCGCCGCTACGGTTTCGGTTTCCCGCACTTTGGTGCGTAGCAGATTGAGCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGC  >  NC_000913/2573625‑2573891                                                                                                                                        |                                                                                                                                    gCGTAATACTTCAAAGCTGCGGGTGATAAAGACCTGCGCCGCTACGGTTTCGGTTTCCCGCACTTTGGTGCGTAGCAGATTGAGCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCAAGAt                                                                                                                                 <  2:310856/140‑1 (MQ=255)           tCAAAGCTGCGGGTGATAAAGACCTGCGCCGCTACGGTTTCGGTTTCCCGCACTTTGGTGCGTAGCAGATTGAGCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCAAGATAGCGCAGCgg                                                                                                                       <  2:596/140‑1 (MQ=255)                    cGGGTGATAAAGACCTGCGCCGCTACGGTTTCGGTTTCCCGCACTTTGGTGCGTAGCAGATTGAGCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCAAGATAGCGCAGCGGCTGATgaga                                                                                                              >  1:723084/1‑140 (MQ=255)                        tGATAAAGACCTGCGCCGCTACGGTTTCGGTTTCCCGCACTTTGGTGCGTAGCAGATTGAGCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGACACCAGATGATCGTGGTCAAGATAGCGCAGCGGCTGATGAGAAAGc                                                                                                          <  1:204766/140‑1 (MQ=255)                         gATAAAGACCTGCGCCGCTACGGTTTCGGTTTCCCGCACTTTGGTGCGTAGCAGATTGAGCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCAAGATAGCGCAGCGGCTGATGAGAAAGcc                                                                                                         <  2:956474/140‑1 (MQ=255)                            aaaGACCTGCGCCGCTACGGTTTCGGTTTCCCGCACTTTGGTGCGTAGCAGATTGAGCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCAAGATAGCGCAGCGGCTGATGAGAAAGCCGAt                                                                                                      <  2:327328/140‑1 (MQ=255)                                 ccTGCGCCGCTACGGTTTCGGTTTCCCGCACTTTGGTGCGTAGCAGATTGAGCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCAAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGa                                                                                                 >  2:602148/1‑140 (MQ=255)                                  cTGCGCCGCTACGGTTTCGGTTTCCCGCACTTTGGTGCGTAGCAGATTGAGCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCAAGATAGCGCAGCGGCTGATgag                                                                                                               <  1:293466/125‑1 (MQ=255)                                  cTGCGCCGCTACGGTTTCGGTTTCCCGCACTTTGGTGCGTAGCAGATTGAGCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCAAGATAGCGCAGCGGCTGATgag                                                                                                               >  2:293466/1‑125 (MQ=255)                                     cgccgcTACGGTTTCGGTTTCCCGCACTTTGGTGCGTAGCAGATTGAGCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCAAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCtt                                                                                             >  1:665231/1‑140 (MQ=255)                                                tttcggtttcCCGCACTTTGGTGCGTAGCAGATTGAGCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCAAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGc                                                                                  <  1:548713/140‑1 (MQ=255)                                                     gtttcCCGCACTTTGGTGCGTAGCAGATTGAGCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCAAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGAc                                                                             >  2:864024/1‑140 (MQ=255)                                                          ccGCACTTTGGTGCGTAGCAGATTGAGCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCAAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCg                                                                        >  1:647986/1‑140 (MQ=255)                                                               cTTTGGTGCGTAGCAGATTGAGCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCAAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTgg                                                                   <  2:248431/140‑1 (MQ=255)                                                                  tGGTGCGTAGCAGATTGAGCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCAAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAg                                                                <  2:647986/140‑1 (MQ=255)                                                                      gCGTAGCAGATTGAGCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCAAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCa                                                            <  1:481311/140‑1 (MQ=255)                                                                         tAGCAGATTGAGCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCAAGATAGCGCGGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCg                                                         <  1:585333/140‑1 (MQ=255)                                                                                 tGAGCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCAAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTccccc                                                 >  1:1039582/1‑140 (MQ=255)                                                                                         gcagcaTCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCAAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATc                                         >  1:385217/1‑140 (MQ=255)                                                                                         gcagcaTCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCAAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATc                                         >  2:80001/1‑140 (MQ=255)                                                                                           agcaTCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCAAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCgg                                       <  2:1039582/140‑1 (MQ=255)                                                                                           agcaTCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCAAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCgg                                       <  1:145059/140‑1 (MQ=255)                                                                                            gcaTCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCAAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGgc                                      <  2:385217/140‑1 (MQ=255)                                                                                            gcaTCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCAAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGgc                                      <  1:836232/140‑1 (MQ=255)                                                                                               tCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCAAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGgcgcg                                   <  1:274377/140‑1 (MQ=255)                                                                                                cgcgCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCAAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGgcgcgc                                  <  1:864024/140‑1 (MQ=255)                                                                                                                          aGATGATCGTGGTCAAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGTGCGCATAATGTTGCCAAGACGCGAACGGat        >  2:286267/1‑140 (MQ=255)                                                                                                                               atCGTGGTCAAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCg   <  2:723084/140‑1 (MQ=255)                                                                                                                                tCGTGGTCAAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCgc  >  2:141316/1‑140 (MQ=255)                                                                                                                                   tGGTCAAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACg           >  1:845782/1‑128 (MQ=255)                                                                                                                                   tGGTCAAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACg           <  2:845782/128‑1 (MQ=255)                                                                                                                                        |                                                                                                                                    GCGTAATACTTCAAAGCTGCGGGTGATAAAGACCTGCGCCGCTACGGTTTCGGTTTCCCGCACTTTGGTGCGTAGCAGATTGAGCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGC  >  NC_000913/2573625‑2573891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑