| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,613,481 | T→C | L528L (TTA→CTA) | hyfR → | DNA‑binding transcriptional activator HyfR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,613,481 | 0 | T | C | 97.1% | 117.3 / ‑4.3 | 34 | L528L (TTA→CTA) | hyfR | DNA‑binding transcriptional activator HyfR |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (20/13); total (20/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.12e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
CATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTTTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATCGCGCAATTGACGCCATCCCGACCGAGGCACTACGCCAGTTGATGTCGTGGGATTGGCCGGGCAACGTGCGCGAGCTGGAAAACGTGATTGAGCG > NC_000913/2613348‑2613617 | cATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTCTAGCaa < 2:733051/140‑1 (MQ=255) aTTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTCTAGCaaa > 2:467332/1‑140 (MQ=255) caccaACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTCTAGCAAAACATTTCa > 2:281620/1‑140 (MQ=255) accaACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTCTAGCAAAACATTTCAc < 2:131999/140‑1 (MQ=255) tGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTCTAGCAAAACATTTCACGCaaaaaa > 1:90408/1‑140 (MQ=255) gtgGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTCTAGCAAAACATTTCACGCAAAAAATGgcgc < 1:850353/140‑1 (MQ=255) tgGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTCTAGCAAAACATTTCACGCAAAAAATGgcgcg > 1:757952/1‑140 (MQ=255) aaaTGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTCTAGCAAAACATTTCACGCAAAAAATGGCGCGCCat > 2:62246/1‑140 (MQ=255) aaTGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTCTAGCAAAACATTTCACGCAAAAAATGgcgc < 1:109761/134‑1 (MQ=255) aaTGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTCTAGCAAAACATTTCACGCAAAAAATGgcgc > 2:109761/1‑134 (MQ=255) gAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTCTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATcg < 1:169814/140‑1 (MQ=255) aaGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTCTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATcg < 2:1026414/139‑1 (MQ=255) aaGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTCTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATcg > 1:1026414/1‑139 (MQ=255) gTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTCTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATcgcg < 2:937891/131‑1 (MQ=255) gTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTCTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATcgcg > 1:937891/1‑131 (MQ=255) ttATCGCCTGAATGTCTTCCCACTGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTCTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATCGCGCAATTGACGCCATCCCGACCGAGGCAc < 1:467332/140‑1 (MQ=255) tCGCCTGAATGTCTTCCCACTGGGATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTCTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATCGCGCAATTGACGCCATCCCGACCGAGGCACTAc > 2:625570/1‑140 (MQ=255) tGAATGTCTTCCCACTGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTCTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATCGCGCAATTGACGCCATCCCGACCGAGGCACTACGCCAg > 1:59308/1‑140 (MQ=255) tGAATGTCTTCCCACTGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTCTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATCGCGCAATTGACGCCATCCCGACCGAGGCACTACGCCAg > 1:543647/1‑140 (MQ=255) tGAATGTCTTCCCACTGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTCTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATCGCGCAATTGACGCCATCCCGACCGAGGCACTACGCCAg > 2:433545/1‑140 (MQ=255) aTGTCTTCCCACTGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTCTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATCGCGCAATTGACGCCATCCCGACCGAGGCACTACGCCAGTTg < 2:757952/140‑1 (MQ=255) tCCCACTGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTTTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATCGCGCAATTGACGCCATCCCGACCGAGGCACTACGCCAGTTGATGTCg < 2:131320/140‑1 (MQ=255) tGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTCTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATCGCGCAATTGACGCCATCCCGACCGAGGCACTACGCCAGTTGATGTCGTGGGAt > 1:389723/1‑140 (MQ=255) tGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTCTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATCGCGCAATTGACGCCATCCCGACCGAGGCACTACGCCAGTTGATGTCGTGGGAt > 1:363058/1‑140 (MQ=255) gAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTCTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATCGCGCAATTGACGCCATCCCGACCGAGGCACTACGCCAGTTGATGTCGTGGGATTg > 1:633269/1‑140 (MQ=255) cgcgACCGTCCGGAAGATATCCCTCTTCTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATCGCGCAATTGACGCCATCCCGACCGAGGCACTACGCCAGTTGATGTCGTGGGATTGGCCGGGCAACGTgcg < 2:184772/140‑1 (MQ=255) cgcgACCGTCCGGAAGATATCCCTCTTCTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATCGCGCAATTGACGCCATCCCGACCGAGGCACTACGCCAGTTGATGTCGTGGGATTGGCCGGGCAACGTgcg < 2:59308/140‑1 (MQ=255) cGGAAGATATCCCTCTTCTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATCGCGCAATTGACGCCATCCCGACCGAGGCACTACGCCAGTTGATGTCGTGGGATTGGCCGGGCAACGTGCGCGAGCTGGaa > 2:833521/1‑140 (MQ=255) gAAGATATCCCTCTTCTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATCGCGCAATTGACGCCATCCCGACCGAGGCACTACGCCAGTTGATGTCGTGGGATTGGCCGGGCAACGTGCGCGAGCTGGaaaa > 2:994661/1‑140 (MQ=255) atCCCTCTTCTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATCGCGCAATTGACGCCATCCCGACCGAGGCACTACGCCAGTTGATGTCGTGGGATTGGCCGGGCAACGTGCGCGAGCTGGAAAACGTGAt > 1:464820/1‑140 (MQ=255) atCCCTCTTCTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATCGCGCAATTGACGCCATCCCGACCGAGGCACTACGCCAGTTGATGTCGTGGGATTGGCCGGGCAACGTGCGCGAGCTGGAAAACGTGAt > 2:905634/1‑140 (MQ=255) atCCCTCTTCTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATCGCGCAATTGACGCCATCCCGACCGAGGCACTACGCCAGTTGATGTCGTGGGATTGGCCGGGCAACGTGCGCGAGCTGGAAAACGTGAt > 1:928106/1‑140 (MQ=255) ctTCTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATCGCGCAATTGACGCCATCCCGGCCGAGGCACTACGCCAGTTGATGTCGTGGGATTGGCCGGGCAACGTGCGCGAGCTGGAAAACGTGATTGAgcg < 1:153758/140‑1 (MQ=255) ctTCTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATCGCGCAATTGACGCCATCCCGACCGAGGCACTACGCCAGTTGATGTCGTGGGATTGGCCGGGCAACGTGCGCGAGCTGGAAAACGTGATTGAgcg < 1:625570/140‑1 (MQ=255) | CATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTTTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATCGCGCAATTGACGCCATCCCGACCGAGGCACTACGCCAGTTGATGTCGTGGGATTGGCCGGGCAACGTGCGCGAGCTGGAAAACGTGATTGAGCG > NC_000913/2613348‑2613617 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |