Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,645,263	G→A	A688V (GCC→GTC)	pbpC ←	peptidoglycan glycosyltransferase PbpC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,645,263	0	G	A	100.0%	102.2 / NA	30	A688V (GCC→GTC)	pbpC	peptidoglycan glycosyltransferase PbpC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/14);  total (16/14)

GAAACCACCAGCGTTCACCTGCCCCTCCACTTGATTGCAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGGCATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGCGCGACGTTCTGATGCGGGCAGCCAGGGTTCCAGCGGTAGCGGCCAGACATTTATCATTTCTTGTCGCGCTTGCGGGCAATC  >  NC_000913/2645141‑2645396                                                                                                                           |                                                                                                                                      gAAACCACCAGCGTTCACCTGCCCCTCCACTTGATTGCAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGc                                                                                                                      <  2:159601/140‑1 (MQ=255)             gTTCACCTGCCCCTCCACTTGATTGCAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTc                                                                                                          <  2:79936/140‑1 (MQ=255)              ttCACCTGCCCCTCCACTTGATTGCAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCg                                                                                                         >  2:726942/1‑140 (MQ=255)                     gCCCCTCCACTTGATTGCAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCgg                                                                                                  <  2:341615/140‑1 (MQ=255)                     gCCCCTCCACTTGATTGCAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCgg                                                                                                  >  1:491148/1‑140 (MQ=255)                      ccccTCCACTTGATTGCAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCggt                                                                                                 >  2:477731/1‑140 (MQ=255)                           ccACTTGATTGCAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTaa                                                                                            >  2:245908/1‑140 (MQ=255)                           ccACTTGATTGCAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTaa                                                                                            >  1:961616/1‑140 (MQ=255)                                   ttGCAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTAAGCGCACAg                                                                                    >  1:346536/1‑140 (MQ=255)                                     gCAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTAAGCGCACAgcg                                                                                  <  1:207389/140‑1 (MQ=255)                                      cAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTaa                                                                                            <  2:83733/129‑1 (MQ=255)                                      cAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTaa                                                                                            >  1:83733/1‑129 (MQ=255)                                       aaCGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGcgcg                                                                                <  1:595203/140‑1 (MQ=255)                                        aCGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGCGCGa                                                                               <  1:424194/140‑1 (MQ=255)                                        aCGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGCGCGa                                                                               <  1:385883/140‑1 (MQ=255)                                            cAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGCGCGACGtt                                                                           >  1:349961/1‑140 (MQ=255)                                                    cTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGCGCGACGTTCTGATGCg                                                                   <  1:477731/140‑1 (MQ=255)                                                      tCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGCGCGACGTTCTGATGCggg                                                                 <  1:888353/140‑1 (MQ=255)                                                                  ggTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGCGCGACGTTCTGATGCGGGCAGCCAGGGTTc                                                     >  1:1006279/1‑140 (MQ=255)                                                                  ggTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGCGCGACGTTCTGATGCGGGCAGCCAGGGGTc                                                     >  1:674617/1‑140 (MQ=255)                                                                   gTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGCGCGACGTTCTGATGCGGGCAGCCAGGGGTcc                                                    >  2:816995/1‑140 (MQ=255)                                                                          tttAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGCGCGACGTTCTGATGCGGGCAGCCAGGGTTCCAGCGGTa                                             >  2:274700/1‑140 (MQ=255)                                                                              ataatCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGCGCGACGTTCTGATGCGGGCAGCCAGGGTTCCAGCGGTAGCgg                                         >  2:1031669/1‑140 (MQ=255)                                                                               taatCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGCGCGACGTTCTGATGCGGGCAGCCAGGGTTCCAGCGGTAGCGGc                                        <  1:245908/140‑1 (MQ=255)                                                                                 atCGCGCCATCGCGAACGCCTGTCAGCTGAAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGGGCGACGTTCTGATGCGGGCAGCCAGGGTTCCAGCGGTAGCgggct                                      <  2:77976/140‑4 (MQ=255)                                                                                  tCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGCGCGACGTTCTGATGCGGGCAGCCAGGGTTCCAGCGGTAGCGGCCAg                                     >  2:25492/1‑140 (MQ=255)                                                                                         aTCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGCGCGACGTTCTGATGCGGGCAGCCAGGGTTCCAGCGGTAGCGGCCAGACATTTa                              <  2:489452/140‑1 (MQ=255)                                                                                           cgcgAACGCCTGTCAGCTGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGCGCGACGTTCTGATGCGGGCAGCCAGGGTTCCAGCGGTAGCGGCCAGACATTTATc                            <  1:726942/140‑1 (MQ=255)                                                                                                            tGCAGCGGGAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGCGCGACGTTCTGATGCGGGCAGCCAGGGTTCCAGCGGTAGCGGCCAGACATTTATCATTTCTTGTCGCGCTTg           >  2:310541/1‑140 (MQ=255)                                                                                                                   ggAGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGCGCGACGTTCTGATGCGGGCAGCCAGGGTTCCAGCGGTAGCGGCCAGACATTTATCATTTCTTGTCGCGCTTGCGGGCaa    >  1:255631/1‑140 (MQ=255)                                                                                                                     aGCTGGACATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGCGCGACGTTCTGATGCGGGCAGCCAGGGTTCCAGCGGTAGCGGCCAGACATTTATCATTTCTTGTCGCGCTTGCGGGCAATc  <  1:25492/140‑1 (MQ=255)                                                                                                                           |                                                                                                                                      GAAACCACCAGCGTTCACCTGCCCCTCCACTTGATTGCAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGGCATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGCGCGACGTTCTGATGCGGGCAGCCAGGGTTCCAGCGGTAGCGGCCAGACATTTATCATTTCTTGTCGCGCTTGCGGGCAATC  >  NC_000913/2645141‑2645396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑