Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,723,976	A→G	H417R (CAC→CGC)	pssA →	phosphatidylserine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,723,976	0	A	G	100.0%	311.3 / NA	83	H417R (CAC→CGC)	pssA	phosphatidylserine synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (40/43);  total (40/43)

CGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTT  >  NC_000913/2723839‑2724111                                                                                                                                          |                                                                                                                                        cGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCt                                                                                                                                       >  2:709603/1‑140 (MQ=255)   gTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTAt                                                                                                                                     >  1:92051/1‑140 (MQ=255)   gTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTAt                                                                                                                                     >  2:410014/1‑140 (MQ=255)   gTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTAt                                                                                                                                     >  2:670264/1‑140 (MQ=255)       taaCCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATcgcg                                                                                                                                 <  1:549423/140‑1 (MQ=255)       taaCCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATcgcg                                                                                                                                 <  2:1038271/140‑1 (MQ=255)       taaCCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATcgcg                                                                                                                                 <  2:632859/140‑1 (MQ=255)        aaCCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGa                                                                                                                                <  1:1027117/140‑1 (MQ=255)          ccTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATc                                                                                                                              <  1:65412/140‑1 (MQ=255)           cTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATcgcg                                                                                                                                 <  2:730746/136‑1 (MQ=255)           cTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATcgcg                                                                                                                                 >  1:730746/1‑136 (MQ=255)           cTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATcgcg                                                                                                                                 >  1:953070/1‑136 (MQ=255)           cTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATcgcg                                                                                                                                 <  2:953070/136‑1 (MQ=255)             gAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGc                                                                                                                           >  1:304556/1‑140 (MQ=255)              aaCCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATcgc                                                                                                                                  <  1:570447/132‑1 (MQ=255)              aaCCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATcgc                                                                                                                                  >  2:570447/1‑132 (MQ=255)              aaCCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCa                                                                                                                          <  1:460156/140‑1 (MQ=255)              aaCCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCa                                                                                                                          <  1:670264/140‑1 (MQ=255)              aaCCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCa                                                                                                                          >  2:923348/1‑140 (MQ=255)              aaCCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCa                                                                                                                          >  2:925966/1‑140 (MQ=255)                  cgcgcgCCTGGCGTCTGGATCTGGAAAACGCCATGTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCATTAAGCGCTATCGCGATCTGGAAAGt                                                                                                                      >  2:939541/1‑140 (MQ=255)                    cgcgcCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTAt                                                                                                                    >  2:531319/1‑140 (MQ=255)                    cgcgcCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTAt                                                                                                                    >  1:197574/1‑140 (MQ=255)                    cgcgcCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTAt                                                                                                                    >  1:212250/1‑140 (MQ=255)                     gcgcCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTAtt                                                                                                                   >  1:13495/1‑140 (MQ=255)                       gcCTAGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGc                                                                                                                 <  2:976031/140‑1 (MQ=255)                                  ggatctggaAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCgg                                                                                                      <  1:1053973/140‑1 (MQ=255)                                      ctggaAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGaa                                                                                                  <  1:410014/140‑1 (MQ=255)                                        ggaAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAgg                                                                                                >  1:494052/1‑140 (MQ=255)                                        ggaAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCAAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAgg                                                                                                <  1:359995/140‑1 (MQ=255)                                              cGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTa                                                                                          <  1:743411/140‑1 (MQ=255)                                                             cGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGtt                                                                           >  2:460075/1‑140 (MQ=255)                                                             cGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGtt                                                                           >  2:290939/1‑140 (MQ=255)                                                                 ccGCAACTTGGGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTgcg                                                                       <  2:207250/140‑1 (MQ=255)                                                                 ccGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTgcg                                                                       <  2:13495/140‑1 (MQ=255)                                                                  cGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTgcgc                                                                      <  1:625531/140‑1 (MQ=255)                                                                      aCTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTa                                                                  <  1:673382/140‑1 (MQ=255)                                                                          gAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCg                                                              >  2:729080/1‑140 (MQ=255)                                                                          gAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCg                                                              <  1:729080/140‑1 (MQ=255)                                                                          gAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCg                                                              >  1:474635/1‑140 (MQ=255)                                                                          gAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCg                                                              >  2:543378/1‑140 (MQ=255)                                                                             cTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCAt                                                           <  1:925966/140‑1 (MQ=255)                                                                               ggCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGA‑‑TGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGAc                                                       >  2:765764/1‑140 (MQ=255)                                                                                gcgcCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGa                                                        <  1:531319/140‑1 (MQ=255)                                                                                 cgcCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGAc                                                       >  2:218802/1‑140 (MQ=255)                                                                                 cgcCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGAc                                                       >  1:404232/1‑140 (MQ=255)                                                                                  gcCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGAcc                                                      <  2:304556/140‑1 (MQ=255)                                                                                    cacaGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGa                                                    <  2:566483/140‑1 (MQ=255)                                                                                    cacaGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGa                                                    <  2:212250/140‑1 (MQ=255)                                                                                         cGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGattaat                                               <  1:554971/140‑1 (MQ=255)                                                                                             agaaagaaCTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGc                                           >  2:133365/1‑140 (MQ=255)                                                                                             agaaagaaCTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGc                                           >  2:185643/1‑140 (MQ=255)                                                                                               aaagaaCTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCg                                         <  1:602953/140‑1 (MQ=255)                                                                                               aaagaaCTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCg                                         <  2:494052/140‑1 (MQ=255)                                                                                                aagaaCTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGc                                        <  1:457818/140‑1 (MQ=255)                                                                                                 agaaCTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCa                                       >  1:311166/1‑140 (MQ=255)                                                                                                      tGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTg                                  <  2:197574/140‑1 (MQ=255)                                                                                                       ggAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGt                                 <  1:218802/140‑1 (MQ=255)                                                                                                        gAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTa                                >  2:711638/1‑140 (MQ=255)                                                                                                        gAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTa                                >  2:521550/1‑140 (MQ=255)                                                                                                          gCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAAt                              >  1:122409/1‑140 (MQ=255)                                                                                                            tGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATca                            <  2:130211/140‑1 (MQ=255)                                                                                                            tGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATca                            <  1:381979/140‑1 (MQ=255)                                                                                                            tGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATca                            >  1:215620/1‑140 (MQ=255)                                                                                                              aTCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATcaca                          >  1:893361/1‑140 (MQ=255)                                                                                                                ccGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAAc                        >  1:390286/1‑140 (MQ=255)                                                                                                                 cgcgAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAAcc                       <  2:474635/140‑1 (MQ=255)                                                                                                                 cgcgAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAAcc                       <  2:390286/140‑1 (MQ=255)                                                                                                                 cgcgAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAAcc                       <  2:122409/140‑1 (MQ=255)                                                                                                                 cgcgAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAAcc                       <  2:611536/140‑1 (MQ=255)                                                                                                                 cgcgAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAAcc                       <  1:189397/140‑1 (MQ=255)                                                                                                                  gcgAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAAccc                      <  2:893361/140‑1 (MQ=255)                                                                                                                  gcgAGCATACCACCATCGTTAAGCGCTATCGCGA‑‑TGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCg                    <  1:765764/140‑1 (MQ=255)                                                                                                                   cgAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAAcccc                     >  2:56192/1‑140 (MQ=255)                                                                                                                      gCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTc                  <  1:551662/140‑1 (MQ=255)                                                                                                                          accaccATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGt              >  2:483177/1‑140 (MQ=255)                                                                                                                                 tCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGgt       <  1:460075/140‑1 (MQ=255)                                                                                                                                  cGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGgtt      >  1:909558/1‑140 (MQ=255)                                                                                                                                    ttAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGgtttg    >  2:779479/1‑140 (MQ=255)                                                                                                                                      aaGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCTCATCGATCGATTAATTAGCCGCATGCTGTAATCACAACCCCGTCCTGTACGGGgtttgtt  >  1:767145/1‑140 (MQ=255)                                                                                                                                      aaGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTcc                 <  2:72561/125‑1 (MQ=255)                                                                                                                                      aaGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTcc                 >  1:72561/1‑125 (MQ=255)                                                                                                                                      aaGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGgtttgtt  >  1:25945/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        CGGTAATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTT  >  NC_000913/2723839‑2724111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑