Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,725,583 G→A S55F (TCC→TTC)  kgtP ← alpha‑ketoglutarate:H(+) symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,725,5830GA100.0% 256.6 / NA 71S55F (TCC→TTC) kgtPalpha‑ketoglutarate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/42);  total (29/42)

CACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGT  >  NC_000913/2725447‑2725710
                                                                                                                                        |                                                                                                                               
cacCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCgaaag                                                                                                                              <  2:362711/140‑1 (MQ=255)
cacCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCgaaag                                                                                                                              >  1:774792/1‑140 (MQ=255)
cacCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCATATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCgaaag                                                                                                                              >  1:289222/1‑140 (MQ=255)
 acCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCgaaagg                                                                                                                             >  2:334155/1‑140 (MQ=255)
   cGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCgaaaggga                                                                                                                           >  2:506329/1‑140 (MQ=255)
   cGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCgaaaggga                                                                                                                           >  2:261052/1‑140 (MQ=255)
     aTAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCgaaagggaag                                                                                                                         >  2:997424/1‑140 (MQ=255)
     aTAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCgaaagggaag                                                                                                                         >  2:524527/1‑140 (MQ=255)
       aaCAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGgaagaa                                                                                                                       <  2:782679/140‑1 (MQ=255)
       aaCAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGgaagaa                                                                                                                       <  1:524527/140‑1 (MQ=255)
         cAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAagaaga                                                                                                                     >  1:557235/1‑140 (MQ=255)
          aGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGAt                                                                                                                    <  1:334155/140‑1 (MQ=255)
          aGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGAt                                                                                                                    <  2:774792/140‑1 (MQ=255)
            cATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGAtgt                                                                                                                  >  1:663856/1‑140 (MQ=255)
             aTCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGAtgtg                                                                                                                 >  1:865572/1‑140 (MQ=255)
             aTCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGAtgtg                                                                                                                 >  2:478574/1‑140 (MQ=255)
             aTCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGAtgtg                                                                                                                 >  2:510896/1‑140 (MQ=255)
             aTCGATTTTTTGCGACCATGTTTATCGGCTATGCGACCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGAtgtg                                                                                                                 >  2:974891/1‑140 (MQ=255)
              tCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTgg                                                                                                                <  2:9618/140‑1 (MQ=255)
              tCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTgg                                                                                                                <  1:622936/140‑1 (MQ=255)
               cGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTggg                                                                                                               <  1:224383/140‑1 (MQ=255)
               cGATTTTTTCCGACCTTGTTTATCGATGCCGGGGCCAAATGCCAAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACTCCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTggg                                                                                                               <  2:851818/140‑1 (MQ=255)
                   tttttGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTGGGCaaa                                                                                                           <  2:717210/140‑1 (MQ=255)
                        gCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTaga                                                                                                      <  2:349375/140‑1 (MQ=255)
                        gCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTaga                                                                                                      <  1:57984/140‑1 (MQ=255)
                        gCGACCATGTTTATCGGCTATGCGGCCAAACAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTaga                                                                                                      <  2:649630/140‑1 (MQ=255)
                            ccATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCATATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTAGAGTGa                                                                                                  <  2:289222/140‑1 (MQ=255)
                              aTGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAAc                                                                                                <  1:478574/140‑1 (MQ=255)
                                     tCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGa                                                                                         <  2:269944/140‑1 (MQ=255)
                                      cGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAg                                                                                        <  1:506329/140‑1 (MQ=255)
                                      cGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAg                                                                                        <  2:201194/140‑1 (MQ=255)
                                      cGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAg                                                                                        <  2:865572/140‑1 (MQ=255)
                                      cGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAg                                                                                        >  2:61628/1‑140 (MQ=255)
                                          tATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTa                                                                                      >  1:117572/1‑138 (MQ=255)
                                          tATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTa                                                                                      <  2:117572/138‑1 (MQ=255)
                                          tATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGa                                                                                    >  2:329325/1‑140 (MQ=255)
                                           aTGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAg                                                                                        <  2:544959/135‑1 (MQ=255)
                                           aTGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAg                                                                                        >  1:544959/1‑135 (MQ=255)
                                           aTGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAc                                                                                   >  1:784104/1‑140 (MQ=255)
                                           aTGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAc                                                                                   >  2:23156/1‑140 (MQ=255)
                                           aTGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCACGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGAc                                                                                   >  2:945434/1‑140 (MQ=255)
                                            tGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTAGAGTg                                                                                                   >  2:582808/1‑123 (MQ=255)
                                            tGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTAGAGTg                                                                                                   <  1:582808/123‑1 (MQ=255)
                                            tGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACa                                                                                  >  2:602434/1‑140 (MQ=255)
                                              cGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATa                                                                                >  2:348457/1‑140 (MQ=255)
                                               ggCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAg                                                                               <  1:602434/140‑1 (MQ=255)
                                                      tAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGa                                                                        >  1:1000235/1‑140 (MQ=255)
                                                        gCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAAc                                                                      <  2:557235/140‑1 (MQ=255)
                                                              cGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCg                                                                <  2:322708/140‑1 (MQ=255)
                                                              cGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCg                                                                <  1:50825/140‑1 (MQ=255)
                                                               gCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGa                                                               <  2:403124/140‑1 (MQ=255)
                                                               gCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGa                                                               <  1:974891/140‑1 (MQ=255)
                                                                ccTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGAc                                                              <  2:337099/140‑1 (MQ=255)
                                                                ccTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGAc                                                              <  2:198513/140‑1 (MQ=255)
                                                                   aTTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAg                                                           <  1:261052/140‑1 (MQ=255)
                                                                    ttGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGa                                                          <  2:663856/140‑1 (MQ=255)
                                                                    ttGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGa                                                          <  2:1000235/140‑1 (MQ=255)
                                                                        gcgcATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTAc                                                      >  1:802550/1‑140 (MQ=255)
                                                                        gcgcATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTAc                                                      >  2:295584/1‑140 (MQ=255)
                                                                            aTCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGGAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGa                                                  >  1:421516/1‑140 (MQ=255)
                                                                                ggAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAagag                                              <  1:329325/140‑1 (MQ=255)
                                                                                ggAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTGGACAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAagag                                              <  1:510896/140‑1 (MQ=255)
                                                                                  aaTCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGc                                            >  1:326296/1‑140 (MQ=255)
                                                                                      ccGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGccccc                                        <  1:613141/140‑1 (MQ=255)
                                                                                      ccGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGccccc                                        >  2:613141/1‑140 (MQ=255)
                                                                                      ccGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGccccc                                        >  2:511340/1‑140 (MQ=255)
                                                                                                        ctgTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATgcgg                      >  1:535860/1‑140 (MQ=255)
                                                                                                          gTTTGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATgcggcg                    <  2:587795/140‑1 (MQ=255)
                                                                                                            ttGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGcgac                  <  2:535860/140‑1 (MQ=255)
                                                                                                             tGTAGTAGTTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGcgacg                 <  2:477578/140‑1 (MQ=255)
                                                                                                                  tagtTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTAt            >  1:824945/1‑140 (MQ=255)
                                                                                                                  tagtTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTAt            <  1:164468/140‑1 (MQ=255)
                                                                                                                  tagtTGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTAt            <  1:23156/140‑1 (MQ=255)
                                                                                                                      tGAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCact        <  2:784104/140‑1 (MQ=255)
                                                                                                                       gAGTCGTCGTGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCacta       >  2:259451/1‑140 (MQ=255)
                                                                                                                         gtcgtcGTGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATACAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCactact     >  2:945159/1‑140 (MQ=255)
                                                                                                                            gtcgtGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCactact     >  1:795149/1‑137 (MQ=255)
                                                                                                                            gtcgtGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCactact     <  2:795149/137‑1 (MQ=255)
                                                                                                                            gtcgtGTTCCCGAAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGt  >  1:445485/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                               
CACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGGCGCATCAGGAATCCCGCAGCAAAAACACCTGCTGTTTGTAGTAGTTGAGTCGTCGTGTTCCCGGAAGGGAAGAAGATGTGGGCAAAGTAGAGTGAACAGAACGAGTAGACATAGAAATCGAACCACTCGACCAGATTACCTGAAGAGGCCCCCACAATCGCCCAAATGCGGCGACGAGTATCACTACTTGT  >  NC_000913/2725447‑2725710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑