Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,735,777	G→A	P79S (CCG→TCG)	rluD ←	23S rRNA pseudouridine(1911/1915/1917) synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,735,777	0	G	A	100.0%	247.4 / NA	69	P79S (CCG→TCG)	rluD	23S rRNA pseudouridine(1911/1915/1917) synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (40/29);  total (40/29)

AATGAAGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGGTTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTGATGGCAACCTGCTCGCCACCCAATACTTTTTCTTTCGGCTTATCACAAACTTTGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGA  >  NC_000913/2735648‑2735912                                                                                                                                  |                                                                                                                                        aaTGAAGCAACGCATTCAGTACCGCGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGCGATATCCTGCGATTCAAAAcgc                                                                                                                               >  2:735357/1‑140 (MQ=255)  aTGAAGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGATTCAAAAcgcg                                                                                                                              >  1:1049154/1‑140 (MQ=255)     aaGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGATTCAAAACGCGctt                                                                                                                           <  2:822768/140‑1 (MQ=255)      aGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGATTCAAAACGCGcttc                                                                                                                          <  2:140067/140‑1 (MQ=255)      aGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGATTCAAAACGCGcttc                                                                                                                          <  2:53565/140‑1 (MQ=255)       gCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGATTCaaaa                                                                                                                                  <  2:242806/131‑1 (MQ=255)       gCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGATTCaaaa                                                                                                                                  >  1:242806/1‑131 (MQ=255)          aCGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGATTCaaaa                                                                                                                                  >  1:747277/1‑128 (MQ=255)          aCGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGATTCaaaa                                                                                                                                  <  2:747277/128‑1 (MQ=255)          aCGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGATTCAAAACGCGcttcttct                                                                                                                      >  2:145286/1‑140 (MQ=255)                    tACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAg                                                                                                            >  2:355647/1‑140 (MQ=255)                      ccGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCg                                                                                                          <  2:124565/140‑1 (MQ=255)                       cGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGt                                                                                                         <  1:21438/140‑1 (MQ=255)                       cGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGt                                                                                                         >  1:745970/1‑140 (MQ=255)                         tGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTg                                                                                                       >  1:438818/1‑140 (MQ=255)                         tGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTg                                                                                                       >  2:77876/1‑140 (MQ=255)                         tGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTg                                                                                                       >  1:70195/1‑140 (MQ=255)                         tGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTg                                                                                                       >  2:303756/1‑140 (MQ=255)                         tGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTg                                                                                                       >  2:856292/1‑140 (MQ=255)                              tCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTGATGGc                                                                                                  <  1:691918/140‑1 (MQ=255)                              tCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTGATGGc                                                                                                  >  2:88588/1‑140 (MQ=255)                               ccGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTGATGGCa                                                                                                 <  1:355647/140‑1 (MQ=255)                                     tACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTGATGGCAACCTGc                                                                                           >  1:853200/1‑140 (MQ=255)                                        ccGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTGATGGCAACCTGCTCg                                                                                        <  1:304204/140‑1 (MQ=255)                                         cgcgCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTGATGGCAACCTGCTCGc                                                                                       <  2:222183/140‑1 (MQ=255)                                            gcCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTGATGGCAACCTGCTCGCCAc                                                                                    <  2:70195/140‑1 (MQ=255)                                             ccAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTGATGGCAACCTGCTCGCCAcc                                                                                   >  1:628667/1‑140 (MQ=255)                                              cAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTGATGGCAACCTGCTCGCCAccc                                                                                  <  1:303756/140‑1 (MQ=255)                                              cAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTGATGGCAACCTGCTCGCCAccc                                                                                  <  1:77876/140‑1 (MQ=255)                                                ggATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTGATGGCAACCTGCTCGCCACCCaa                                                                                <  1:828252/140‑1 (MQ=255)                                                          ccaGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTGATGGCAACCTGCTCGCCACCCAATACTTtttct                                                                      >  1:571627/1‑140 (MQ=255)                                                           caGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTGATGGCAACCTGCTCGCCACCCAATACTTtttctt                                                                     >  2:513946/1‑140 (MQ=255)                                                            aGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTGATGGCAACCTGCTCGCCACCCAATACTTtttcttt                                                                    <  1:739553/140‑1 (MQ=255)                                                               tCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTGATGGCAACCTGCTCGCCACCCAATACTTTTTCTTTCgg                                                                 >  2:1034138/1‑140 (MQ=255)                                                                 gcgcgGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTGATGGCAACCTGCTCGCCACCCAATACTTTTTCTTTCGGCt                                                               <  1:88588/140‑1 (MQ=255)                                                                  cgcgGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTGATGGCAACCTGCTCGCCACCCAATACTTTTTCTTTCGGCtt                                                              >  2:135230/1‑140 (MQ=255)                                                                  cgcgGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTGATGGCAACCTGCTCGCCACCCAATACTTTTTCTTTCGGCtt                                                              >  2:13290/1‑140 (MQ=255)                                                                  cgcgGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTGATGGCAACCTGCTCGCCACCCAATACTTTTTCTTTCGGCt                                                               >  1:1028688/1‑139 (MQ=255)                                                                  cgcgGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTGATGGCAACCTGCTCGCCACCCAATACTTTTTCTTTCGGCt                                                               <  2:1028688/139‑1 (MQ=255)                                                                             aaTGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTGATGGCAACCTGCTCGCCACCCAATACTTTTTCTTTCGGCTTATCACAAACtt                                                   <  2:628667/140‑1 (MQ=255)                                                                             aaTGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGGTGATGGCAACCTGCTCGCCACCCAATACTTCTTCTTTCGGCTTATCACAAACtt                                                   <  1:735357/140‑1 (MQ=255)                                                                                 ataataATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTGATGGCAACCTGCTCGCCACCCAATACTTTTTCTTTCGGCTTATCACAAACTTTGcc                                               >  2:707216/1‑140 (MQ=255)                                                                                 ataataATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTGATGGCAACCTGCTCGCCACCCAATACTTTTTCTTTCGGCTTATCACAAACTTTGcc                                               >  2:639255/1‑140 (MQ=255)                                                                                 ataataATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTGATGGCAACCTGCTCGCCACCCAATACTTTTTCTTTCGGCTTATCACAAACTTTGcc                                               >  1:846305/1‑140 (MQ=255)                                                                                 ataataATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTGATGGCAACCTGCTCGCCACCCAATACTTTTTCTTTCGGCTTATCACAAACTTTGcc                                               >  1:715130/1‑140 (MQ=255)                                                                                  taataaTGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTGATGGCAACCTGCTCGCCACCCAATACTTTTTCTTTCGGCTTATCACAAACTTTGCCg                                              <  1:343120/140‑1 (MQ=255)                                                                                       atGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTGATGGCAACCTGCTCGCCACCCAATACTTTTTCTTTCGGCTTATCACAAACTTTGCCGTTAAc                                         <  2:1049154/140‑1 (MQ=255)                                                                                          tcttcatcttcaTAGACGATATCCAGCGGGATATCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTGATGGCAACCTGCTCGCCACCCAATACTTTTTCTTTCGGCTTATCACAAACTTTGCCGTTAACCAg                                      <  2:716048/140‑1 (MQ=255)                                                                                            ttcatcttcatAGACGATATCCAGCGGGATATCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTGATGGCAACCTGCTCGCCACCCAATACTTTTTCTTTCGGCTTATCACAAACTTTGCCGTTAACCAGCa                                    >  2:894281/1‑140 (MQ=255)                                                                                              catcttcatAGACGATATCCAGCGGGATATCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTGATGGCAACCTGCTCGCCACCCAATACTTTTTCTTTCGGCTTATCACAAACTTTGCCGTTAACCAGCACt                                  <  1:513946/140‑1 (MQ=255)                                                                                              catcttcatAGACGATATCCAGCGGGATATCATGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTGATGACAACCTGCTCGCCACCCAATACTTTTTCTTTCGGCTTATCACAAACTTTGCCGTTAACCAGCACt                                  >  1:635834/1‑140 (MQ=255)                                                                                               atcttcatAGACGATATCCAGCGGGATATCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTGATGGCAACCTGCTCGCCACCCAATACTTTTTCTTTCGGCTTATCACAAACTTTGCCGTTAACCAGCACTc                                 >  2:319266/1‑140 (MQ=255)                                                                                               atcttcatAGACGATATCCAGCGGGATATCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTGATGGCAACCTGCTCGCCACCCAATACTTTTTCTTTCGGCTTATCACAAACTTTGCCGTTAACCAGCACTc                                 >  2:96395/1‑140 (MQ=255)                                                                                                 cttcatAGACGATATCCAGCGGGATATCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTGATGGCAACCTGCTCGCCACCCAATACTTTTTCTTTCGGCTTATCACAAACTTTGCCGTTAACCAGCACTCGc                               <  2:876330/140‑1 (MQ=255)                                                                                                         aCGATATCCAGCGGGATATCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTGATGGCAACCTGCTCGCCACCCAATACTTTTTCTTTCGGCTTATCACAAACTTTGCCGTTAACCAGCACTCGCTGGTCGAg                       >  2:34443/1‑140 (MQ=255)                                                                                                         aCGATATCCAGCGGGATATCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTGATGGCAACCTGCTCGCCACCCAATACTTTTTCTTTCGGCTTATCACAAACTTTGCCGTTAACCAGCACTCGCTGGTCGAg                       >  1:1020266/1‑140 (MQ=255)                                                                                                           gATATCCAGCGGGATATCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTGATGGCAACCTGCTCGCCACCCAATACTTTTTCTTTCGGCTTATCACAAACTTTGCCGTTAACCAGCACTCGCTGGTCGAGGa                     >  1:653952/1‑140 (MQ=255)                                                                                                              atCCAGCGGGATATCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTGATGGCAACCTGCTCGCCACCCAATACTTTTTCTTTCGGCTTATCACAAACTTTGCCGTTAACCAGCACTCGCTGGTCGAGGATcc                  >  1:813949/1‑140 (MQ=255)                                                                                                                   gCGGGATATCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTGATGGCAACCTGCTCGCCACCCAATACTTTTTCTTTCGGCTTATCACAAACTTTGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCt             >  1:43534/1‑140 (MQ=255)                                                                                                                    cGGGATATCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTGATGGCAACCTGCTCGCCACCCAATACTTTTTCTTTCGGCTTATCACAAACTTTGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCtt            >  2:32896/1‑140 (MQ=255)                                                                                                                       gATATCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTGATGGCAACCTGCTCGCCACCCAATACTTTTTCTTTCGGCTTATCACAAACTTTGCCGTTAACCAGCACTCGCTGGTCGAGGa                     >  2:705715/1‑128 (MQ=255)                                                                                                                       gATATCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTGATGGCAACCTGCTCGCCACCCAATACTTTTTCTTTCGGCTTATCACAAACTTTGCCGTTAACCAGCACTCGCTGGTCGAGGa                     <  1:705715/128‑1 (MQ=255)                                                                                                                       gATATCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTGATGGCAACCTGCTCGCCACCCAATACTTTTTCTTTCGGCTTATCACAAACTTTGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTa         >  1:139206/1‑140 (MQ=255)                                                                                                                       gATATCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTGATGGCAACCTGCTCGCCACCCAATACTTTTTCTTTCGGCTTATCACAAACTTTGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTa         >  2:1033573/1‑140 (MQ=255)                                                                                                                           tCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTGATGGCAACCTGCTCGCCACCCAATACTTTTTCTTTCGGCTTATCACAAACTTTGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTcg     <  2:745970/140‑1 (MQ=255)                                                                                                                           tCCTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTGATGGCAACCTGCTCGCCACCCAATACTTTTTCTTTCGGCTTATCACAAACTTTGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTcg     <  1:876597/140‑1 (MQ=255)                                                                                                                            ccTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTGATGGCAACCTGCTCGCCACCCAATACTTTTTCTTTCGGCTTATCACAAACTTTGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTcgc    >  2:475670/1‑140 (MQ=255)                                                                                                                             cTGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTGATGGCAACCTGCTCGCCACCCAATACTTTTTCTTTCGGCTTATCACAAACTTTGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTcgcg   <  2:990858/140‑1 (MQ=255)                                                                                                                              tGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTGATGGCAACCTGCTCGCCACCCAATACTTTTTCTTTCGGCTTATCACAAACTTTGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGa  >  2:289277/1‑140 (MQ=255)                                                                                                                              tGCGATTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTGATGGCAACCTGCTCGCCACCCAATACTTTTTCTTTCGGCTTATCACAAACTTTGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGa  >  2:102842/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                                        AATGAAGCAACGCATTCAGTACCGTGCCATCCGGGTTACCCGCGCCAGGATGTACCACCAGGTCGCGCGGTTTATTAATGATAATAATGTCTTCATCTTCATAGACGATATCCAGCGGGATATCCTGCGGTTCAAAACGCGCTTCTTCTTCAATCTCAGCGTTGATGGCAACCTGCTCGCCACCCAATACTTTTTCTTTCGGCTTATCACAAACTTTGCCGTTAACCAGCACTCGCTGGTCGAGGATCCATTCTTTTATTCGCGA  >  NC_000913/2735648‑2735912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑