Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,749,090	T→C	L106P (CTG→CCG)	yfjD →	putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,749,090	0	T	C	100.0%	191.3 / NA	53	L106P (CTG→CCG)	yfjD	putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/26);  total (27/26)

CGCCTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCTGCAAATTTTGATGATGCCGCTGGTCTGGTTGCTGAATGCTATCACCCGT  >  NC_000913/2748954‑2749226                                                                                                                                         |                                                                                                                                         cGCCTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGt                                                                                                                                       <  2:171154/140‑1 (MQ=255)   ccTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTAt                                                                                                                                     >  1:163437/1‑140 (MQ=255)   ccTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTAt                                                                                                                                     <  2:513811/140‑1 (MQ=255)   ccTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTAt                                                                                                                                     >  2:854130/1‑140 (MQ=255)    cTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTAtt                                                                                                                                    <  2:1019105/140‑1 (MQ=255)    cTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTAtt                                                                                                                                    >  2:908755/1‑140 (MQ=255)       aTAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGc                                                                                                                                 <  1:908755/140‑1 (MQ=255)                gtgtTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTAtt                                                                                                                        >  1:834975/1‑140 (MQ=255)                 tgtTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTg                                                                                                                       >  1:1052008/1‑140 (MQ=255)                 tgtTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTg                                                                                                                       >  2:396872/1‑140 (MQ=255)                     aaTCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGa                                                                                                                   >  1:920051/1‑140 (MQ=255)                           cAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGTGGTATTGCCGAAAACCa                                                                                                             >  2:327617/1‑140 (MQ=255)                           cAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCa                                                                                                             <  1:214247/140‑1 (MQ=255)                           cAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCa                                                                                                             >  1:3765/1‑140 (MQ=255)                               aaCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGc                                                                                                         <  2:834975/140‑1 (MQ=255)                                   tGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCcgcg                                                                                                     <  1:396872/140‑1 (MQ=255)                                   tGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCcgcg                                                                                                     <  2:3765/140‑1 (MQ=255)                                     gTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCt                                                                                                   <  1:482052/140‑1 (MQ=255)                                     gTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCt                                                                                                   >  1:258391/1‑140 (MQ=255)                                     gTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCt                                                                                                   <  1:101220/140‑1 (MQ=255)                                         atatTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTAc                                                                                               <  1:837713/140‑1 (MQ=255)                                               ttGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGaa                                                                                         >  1:512153/1‑140 (MQ=255)                                               ttGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGaa                                                                                         >  1:146021/1‑140 (MQ=255)                                                      cgcgCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCg                                                                                  <  2:568508/140‑1 (MQ=255)                                                      cgcgCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCg                                                                                  <  2:920051/140‑1 (MQ=255)                                                           tCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTAt                                                                             <  1:747716/140‑1 (MQ=255)                                                                            aTGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTctgct                                                            >  1:17876/1‑140 (MQ=255)                                                                              gCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTgg                                                          >  2:431891/1‑140 (MQ=255)                                                                                     tACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCt                                                   >  2:174613/1‑140 (MQ=255)                                                                                      aCGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCTg                                                  <  2:512153/140‑1 (MQ=255)                                                                                       cGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAGCCATTGTCGCGATGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTACTGGCTCCGCTGc                                                 <  2:803588/140‑1 (MQ=255)                                                                                         gCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGTAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCTGCa                                                <  2:964719/139‑1 (MQ=255)                                                                                         gCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCTGCaa                                               >  2:808920/1‑140 (MQ=255)                                                                                         gCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCTGCa                                                >  1:964719/1‑139 (MQ=255)                                                                                            aTGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCTGCAAAtt                                            >  2:142017/1‑140 (MQ=255)                                                                                            aTGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCTGCAAAtt                                            >  1:723764/1‑140 (MQ=255)                                                                                            aTGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCTGCAAAtt                                            >  2:511644/1‑140 (MQ=255)                                                                                            aTGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCTGCAAAtt                                            >  2:520254/1‑140 (MQ=255)                                                                                                       gCAATTGCGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCTGCAAATTTTGATGATGcc                                 >  2:578916/1‑140 (MQ=255)                                                                                                              cGACTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCTGCAAATTTTGATGATGCCGctggtc                          <  1:142017/140‑1 (MQ=255)                                                                                                                 cTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGTGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCTGCAAATTTTGATGATGCCGctggtctgg                       <  1:327617/140‑1 (MQ=255)                                                                                                                 cTGGTGTGCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCTGCAAATTTTGATGATGCCGctggtctgg                       <  1:174613/140‑1 (MQ=255)                                                                                                                     tgtgCTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCTGCAAATTTTGATGATGCCGCTGGTCTGGTTGc                   >  2:561974/1‑140 (MQ=255)                                                                                                                         cTGACTTTTGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCTGCAAATTTTGATGATGCCGCTGGTCTGGTTGCTGaa               >  2:616282/1‑140 (MQ=255)                                                                                                                              ttttGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCTGCAAATTTTGATGATGCCGCTGGTCTGGTTGCTGAATGCTa          >  2:621047/1‑140 (MQ=255)                                                                                                                               tttGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCTGCAAATTTTGATGATGCCGCTGGTCTGGTTGCTGAATGCTAt         <  2:1052008/140‑1 (MQ=255)                                                                                                                               tttGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCTGCAAATTTTGATGATGCCGCTGGTCTGGTTGCTGAATGCTAt         <  2:723764/140‑1 (MQ=255)                                                                                                                               tttGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCTGCAAATTTTGATGATGCCGCTGGTCTGGTTGCTGAATGCTAt         <  2:146021/140‑1 (MQ=255)                                                                                                                               tttGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCTGCAAATTTTGATGATGCCGCTGGTCTGGTTGCTGAATGCTAt         <  1:431891/140‑1 (MQ=255)                                                                                                                               tttGTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCTGCAAATTTTGATGATGCCGCTGGTCTGGTTGCTGAATGCTAt         >  1:250774/1‑140 (MQ=255)                                                                                                                                  gTCGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCTGCAAATTTTGATGATGCCGCTGGTCTGGTTGCTGAATGCTATCAc      <  2:184705/140‑1 (MQ=255)                                                                                                                                    cGTACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCTGCAAATTTTGATGATGCCGCTGGTCTGGTTGCTGAATGCTATCAccc    <  1:640434/140‑1 (MQ=255)                                                                                                                                      tACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCTGCAAATTTTGATGATGCCGCTGGTCTGGTTg                    <  2:101656/122‑1 (MQ=255)                                                                                                                                      tACCGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCTGCAAATTTTGATGATGCCGCTGGTCTGGTTGCTGAATGCTATCACCCGt  >  2:485723/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                         CGCCTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCGCTTATCCGAGTAGTTTTCTGCTGGCTCCGCTGCAAATTTTGATGATGCCGCTGGTCTGGTTGCTGAATGCTATCACCCGT  >  NC_000913/2748954‑2749226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑