Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,810,405	A→G	Y21C (TAT→TGT)	ygaH →	L‑valine exporter subunit YgaH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,810,405	0	A	G	100.0%	264.1 / NA	70	Y21C (TAT→TGT)	ygaH	L‑valine exporter subunit YgaH
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/34);  total (36/34)

CCGTCGCCATTCTGGCAGGCATTGTCTGTGGCTGCCTCACTGCGTTAATCCAGGCATTCTGGCAAGGAGCGCCCGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCACG  >  NC_000913/2810269‑2810539                                                                                                                                         |                                                                                                                                       ccGTCGCCATTCTGGCAGGCATTGTCTGTGGCTGCCTCACTGCGTTAATCCAGGCATTCTGGCAAGGAGCGCCCGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTGttt                                                                                                                                     >  2:798682/1‑140 (MQ=255)   gTCGCCATTCTGGCAGGCATTGTCTGTGGCTGCCTCACTGCGTTAATCCAGGCATTCTGGCAAGGAGCGCCCGATGAGCTAGGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTGTTTGc                                                                                                                                   >  2:928863/1‑140 (MQ=255)       ccATTCTGGCAGGCATTGTCTGTGGCTGCCTCACTGCGTTAATCCAGGCATTCTGGCAAGGAGCGCCCGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTGTTTGCCGCt                                                                                                                               <  2:831845/140‑1 (MQ=255)           tCTGGCAGGCATTGTCTGTGGCTGCCTCACTGCGTTAATCCAGGCATTCTGGCAAGGAGCGCCCGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTGTTTGCCGCTgcgc                                                                                                                           <  1:532888/140‑1 (MQ=255)           tCTGGCAGGCATTGTCTGTGGCTGCCTCACTGCGTTAATCCAGGCATTCTGGCAAGGAGCGCCCGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTGTTTGCCGCTgcgc                                                                                                                           <  2:700476/140‑1 (MQ=255)              ggcaggcaTTGTCTGTGGCTGCCTCACTGCGTTAATCCAGGCATTCTGGCAAGGAGCGCCCGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTg                                                                                                                        <  2:267346/140‑1 (MQ=255)              ggcaggcaTTGTCTGTGGCTGCCTCACTGCGTTAATCCAGGCATTCTGGCAAGGAGCGCCCGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTg                                                                                                                        <  2:77967/140‑1 (MQ=255)                  ggcaTTGTCTGTGGCTGCCTCCCTGCGTTAATCCAGGCATTCTGGCAAGGAGCGCCCGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCgtg                                                                                                                    <  2:666539/140‑1 (MQ=255)                  ggcaTTGTCTGTGGCTGCCTCACTGCGTTAATCCAGGCATTCTGGCAAGGAGCGCCCGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGGGTGGCGAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCgtg                                                                                                                    <  2:139922/140‑1 (MQ=255)                  ggcaTTGTCTGTGGCTGCCTCACTGCGTTAATCCAGGCATTCTGGCAAGGAGCGCCCGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCgtg                                                                                                                    <  1:798682/140‑1 (MQ=255)                  ggcaTTGTCTGTGGCTGCCTCACTGCGTTAATCCAGGCATTCTGGCAAGGAGCGCCCGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCgtg                                                                                                                    <  2:504962/140‑1 (MQ=255)                                   ccTCACTGCGTTAATCCAGGCATTCTGGCAAGGAGCGCCCGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCcca                                                                                                   >  2:716320/1‑140 (MQ=255)                                   ccTCACTGCGTTAATCCAGGCATTCTGGCAAGGAGCGCCCGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCcca                                                                                                   >  2:698755/1‑140 (MQ=255)                                   ccTCACTGCGTTAATCCAGGCATTCTGGCAAGGAGCGCCCGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCcca                                                                                                   >  1:35921/1‑140 (MQ=255)                                         tGCGTTAATCCAGGCATTCTGGCAAGGAGCGCCCGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCaaa                                                                                             >  1:312455/1‑140 (MQ=255)                                             ttAATCCAGGCATTCTGGCAAGGAGCGCCCGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTg                                                                                         >  2:256817/1‑140 (MQ=255)                                             ttAATCCAGGCATTCTGGCAAGGAGCGCCCGATGAGCTATGAGGTTCTACTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTg                                                                                         >  1:622434/1‑140 (MQ=255)                                                aTCCAGGCATTCTGGCAAGGAGCGCCCGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGgcg                                                                                      <  1:928863/140‑1 (MQ=255)                                                    aGGCATTCTGCCAAGGAGCGCCCGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCggt                                                                                  <  1:988685/140‑1 (MQ=255)                                                              gCAAGGAGCGCCCGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTg                                                                        <  1:174477/140‑1 (MQ=255)                                                                aaGGAGCGCCCGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCt                                                                      <  1:170400/140‑1 (MQ=255)                                                                   gAGCGCCCGATGAGCTATGAGGTTCTGCCGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGa                                                                   <  2:886895/140‑1 (MQ=255)                                                                    aGCGCCCGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGac                                                                  >  1:284362/1‑140 (MQ=255)                                                                    aGCGCCCGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCAGCGCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGac                                                                  >  2:731208/1‑140 (MQ=255)                                                                      cgcCCGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGacac                                                                >  1:904247/1‑140 (MQ=255)                                                                        cccGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCa                                                              >  1:426065/1‑140 (MQ=255)                                                                        cccGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCa                                                              >  2:78919/1‑140 (MQ=255)                                                                        cccGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCa                                                              >  1:1058979/1‑140 (MQ=255)                                                                            aTGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTATGCTCGACACCATTgg                                                          >  2:950830/1‑140 (MQ=255)                                                                             tGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGc                                                         <  2:327080/140‑1 (MQ=255)                                                                                gCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTGTTTGCCGGTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATc                                                      >  1:927582/1‑140 (MQ=255)                                                                                   aTGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGcc                                                   <  1:256817/140‑1 (MQ=255)                                                                                      aGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCg                                                >  1:484506/1‑140 (MQ=255)                                                                                       ggTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGa                                               >  2:403223/1‑140 (MQ=255)                                                                                           ctgctgCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATg                                           >  1:902585/1‑140 (MQ=255)                                                                                            tgctgcTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATgc                                          >  1:537376/1‑140 (MQ=255)                                                                                             gctgctTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATgcg                                         <  2:904247/140‑1 (MQ=255)                                                                                              ctgctTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATgcgc                                        >  1:509014/1‑140 (MQ=255)                                                                                                  ttGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTctg                                    <  1:335632/140‑1 (MQ=255)                                                                                                   tGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTctgc                                   <  1:422307/140‑1 (MQ=255)                                                                                                     ggTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTctgctg                                 <  2:426065/140‑1 (MQ=255)                                                                                                      gTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTgg                                <  2:409341/140‑1 (MQ=255)                                                                                                        tACTAGTTGGCGTGGCGAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGct                                       >  1:219132/1‑131 (MQ=255)                                                                                                        tACTAGTTGGCGTGGCGAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGct                                       <  2:219132/131‑1 (MQ=255)                                                                                                          cTAGTTGGCGTGGCGAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGt                            <  1:698755/140‑1 (MQ=255)                                                                                                          cTAGTTGGCGTGGCGAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGt                            <  1:716320/140‑1 (MQ=255)                                                                                                                ggcgtggcgAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTAc                      >  2:521294/1‑140 (MQ=255)                                                                                                                ggcgtggcgAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTAc                      <  2:537376/140‑1 (MQ=255)                                                                                                                ggcgtggcgAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTAc                      >  1:277445/1‑140 (MQ=255)                                                                                                                ggcgtggcgAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTAc                      <  1:109338/140‑1 (MQ=255)                                                                                                                ggcgtggcgAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTAc                      <  2:871907/140‑1 (MQ=255)                                                                                                                ggcgtggcgAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTAc                      >  1:210132/1‑140 (MQ=255)                                                                                                                ggcgtggcgAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTAc                      >  1:153491/1‑140 (MQ=255)                                                                                                                ggcgtggcgAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTAc                      >  1:871907/1‑140 (MQ=255)                                                                                                                ggcgtggcgAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTAc                      <  2:284715/140‑1 (MQ=255)                                                                                                                ggcgtggcgAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTAc                      >  1:685219/1‑140 (MQ=255)                                                                                                                ggcgtggcgAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTAc                      >  2:109338/1‑140 (MQ=255)                                                                                                                ggcgtggcgAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTAc                      >  1:611993/1‑140 (MQ=255)                                                                                                                  cgtggcgAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCg                    >  2:416736/1‑140 (MQ=255)                                                                                                                  cgtggcgAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCg                    >  1:468650/1‑140 (MQ=255)                                                                                                                  cgtggcgAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCACTCTGCTGGTTGTCTCTACCg                    >  2:164970/1‑140 (MQ=255)                                                                                                                    tggcgAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCa                  >  2:583996/1‑140 (MQ=255)                                                                                                                     ggcgAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCAc                 <  1:554337/140‑1 (MQ=255)                                                                                                                     ggcgAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCAc                 <  1:889666/140‑1 (MQ=255)                                                                                                                     ggcgAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCAc                 <  2:312455/140‑1 (MQ=255)                                                                                                                      gcgAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCAcc                >  2:515188/1‑140 (MQ=255)                                                                                                                       cgAATTATTGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCa               >  1:23381/1‑140 (MQ=255)                                                                                                                          attattGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGaa            <  2:284362/140‑1 (MQ=255)                                                                                                                             attGCTTCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCAGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTg         <  1:731208/140‑1 (MQ=255)                                                                                                                                  ttCCGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCa    <  2:484506/140‑1 (MQ=255)                                                                                                                                    ccGCTGTTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCACg  <  1:571618/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       CCGTCGCCATTCTGGCAGGCATTGTCTGTGGCTGCCTCACTGCGTTAATCCAGGCATTCTGGCAAGGAGCGCCCGATGAGCTATGAGGTTCTGCTGCTTGGGTTACTAGTTGGCGTGGCGAATTATTGCTTCCGCTATTTGCCGCTGCGCCTGCGTGTGGGTAATGCCCGCCCAACCAAACGTGGCGCGGTAGGTATTTTGCTCGACACCATTGGCATCGCCTCGATATGCGCTCTGCTGGTTGTCTCTACCGCACCAGAAGTGATGCACG  >  NC_000913/2810269‑2810539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑