Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,816,960	C→T	A182T (GCC→ACC)	yqaB ←	fructose‑1‑phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,816,960	0	C	T	100.0%	174.6 / NA	50	A182T (GCC→ACC)	yqaB	fructose‑1‑phosphatase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/21);  total (29/21)

ATGACTGATCCCGGAAAGCAACATTGCCACCAGCACGACTTCGGAGTTGCCGGGTAATAGTGTAGCGCTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGCGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACG  >  NC_000913/2816824‑2817093                                                                                                                                         |                                                                                                                                      aTGACTGATCCCGGAAAGCAACATTGCCACCAGCACGACTTCGGAGTTGCCGGGTAATAGTGTAGCGCTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTc                                                                                                                                    <  1:329500/140‑1 (MQ=255)    aCTGATCCCGGAAAGCAACATTGCCACCAGCACGACTTCGGAGTTGCCGGGTAATAGTGTAGCGCTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCAt                                                                                                                                 >  2:22452/1‑140 (MQ=255)    aCTGATCCCGGAAAGCAACATTGCCACCAGCACGACTTCGGAGTTGCCGGGTAATAGTGTAGCGCTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCAt                                                                                                                                 <  1:134388/140‑1 (MQ=255)    aCTGATCCCGGAAAGCAACATTGCCACCAGCACGACTTCGGAGTTGCCGGGTAATAGTGTAGCGCTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCAt                                                                                                                                 >  1:720059/1‑140 (MQ=255)    aCTGATCCCGGAAAGCAACATTGCCACCAGCACGACTTCGGAGTTGCCGGGTAATAGTGTAGCGCTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCAt                                                                                                                                 >  2:70334/1‑140 (MQ=255)              gAAAGCAACATTGCCACCAGCACGACTTCGGAGTTGCCGGGTAATAGTGTAGCGCTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCa                                                                                                                       >  2:646821/1‑140 (MQ=255)              gAAAGCAACATTGCCACCAGCACGACTTCGGAGTTGCCGGGTAATAGTGTAGCGCTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCa                                                                                                                       <  1:646821/140‑1 (MQ=255)              gAAAGCAACATTGCCACCAGCACGACTTCGGAGTTGCCGGGTAATAGTGTAGCGCTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCa                                                                                                                       >  1:613284/1‑140 (MQ=255)              gAAAGCAACATTGCCACCAGCACGACTTCGGAGTTGCCGGGTAATAGAGTAGCGCTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCa                                                                                                                       >  2:593290/1‑140 (MQ=255)               aaaGCAACATTGCCACCAGCACGACTTCGGAGTTGCCGGGTAATAGTGTAGCGCTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCAc                                                                                                                      >  1:275600/1‑140 (MQ=255)                    aaCATTGCCACCAGCACGACTTCGGAGTTGCCGGGTAATAGTGTAGCGCTGAGAAAACTACTGGCAAACAAGGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGgcc                                                                                                                 >  2:737797/1‑140 (MQ=255)                                              gctGCCGGGTAATAGTGTAGCGCTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTc                                                                                       <  2:720059/138‑1 (MQ=255)                                               ttGCCGGGTAATAGTGTAGCGCTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCa                                                                                      <  1:70334/140‑1 (MQ=255)                                                tGCCGGGTAATAGTGTAGCGCTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCaa                                                                                     <  1:904030/140‑1 (MQ=255)                                                tGCCGGGTAATAGTGTAGCGCTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCaa                                                                                     <  2:378423/140‑1 (MQ=255)                                                tGCCGGGTAATAGTGTAGCGCTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCaa                                                                                     <  1:560497/140‑1 (MQ=255)                                                tGCCGGGTAATAGTGTAGCGCTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCaa                                                                                     <  2:86915/140‑1 (MQ=255)                                                  ccGGGTAATAGTGTAGCGCTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAg                                                                                   <  2:833488/140‑1 (MQ=255)                                                  ccGGGTAATAGTGTAGCGCTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAg                                                                                   >  1:1030709/1‑140 (MQ=255)                                                     ggTAATAGTGTAGCGCTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCAGTCACAGCAAGCGAACATACACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGAcc                                                                                <  2:948179/140‑1 (MQ=255)                                                               tAGCGCTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCg                                                                      >  2:15362/1‑140 (MQ=255)                                                                aGCGCTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGt                                                                     >  1:173142/1‑140 (MQ=255)                                                                aGCGCTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATACCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGt                                                                     >  2:371477/1‑140 (MQ=255)                                                                 gcgcTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTc                                                                    >  2:450559/1‑140 (MQ=255)                                                                 gcgcTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTc                                                                    >  1:959982/1‑140 (MQ=255)                                                                 gcgcTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTc                                                                    >  1:511315/1‑140 (MQ=255)                                                                 gcgcTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATACCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTc                                                                    <  1:371477/140‑1 (MQ=255)                                                                  cgcTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCg                                                                   >  1:877355/1‑140 (MQ=255)                                                                  cgcTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCg                                                                   >  2:537282/1‑140 (MQ=255)                                                                     tGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGtt                                                                >  2:680760/1‑140 (MQ=255)                                                                       agaAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGc                                                              >  1:835231/1‑140 (MQ=255)                                                                        gaAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCa                                                             <  1:145284/140‑1 (MQ=255)                                                                        gaAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCa                                                             <  1:647738/140‑1 (MQ=255)                                                                        gaAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCa                                                             <  1:450559/140‑1 (MQ=255)                                                                                 tGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATAc                                                    <  2:613284/140‑1 (MQ=255)                                                                                             agaAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGcaca                                        >  2:814697/1‑140 (MQ=255)                                                                                               aaaaGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCacaac                                      <  1:15362/140‑1 (MQ=255)                                                                                                             ttCACTCACAGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTgg                        >  2:141236/1‑140 (MQ=255)                                                                                                                    acaGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGACATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGt                 <  1:792975/140‑1 (MQ=255)                                                                                                                      aGCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGttt               >  2:860961/1‑140 (MQ=255)                                                                                                                       gCAAGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTa              <  2:511315/140‑1 (MQ=255)                                                                                                                          aGCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATgg           >  2:251596/1‑140 (MQ=255)                                                                                                                           gCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTAt             >  2:818080/1‑137 (MQ=255)                                                                                                                           gCGAACATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTAt             <  1:818080/137‑1 (MQ=255)                                                                                                                              aaCATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGgcg                      <  1:324993/125‑1 (MQ=255)                                                                                                                              aaCATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGgcg                      >  2:324993/1‑125 (MQ=255)                                                                                                                              aaCATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGtt       >  1:596593/1‑140 (MQ=255)                                                                                                                                cATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTg     >  1:167463/1‑140 (MQ=255)                                                                                                                                cATCCACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTg     >  2:909529/1‑140 (MQ=255)                                                                                                                                   ccACGGTGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACg  >  1:1055841/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      ATGACTGATCCCGGAAAGCAACATTGCCACCAGCACGACTTCGGAGTTGCCGGGTAATAGTGTAGCGCTGAGAAAACTACTGGCAAACAACGAGAAAAGCGATAACGCTTCACTCACAGCAAGCGAACATCCACGGCGTCCATGCCTGCTGCACGGGCCGCCTGAATACCGAAATCGGCATCTTCAAAGACCACACACTGCGTCGGTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACG  >  NC_000913/2816824‑2817093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑