Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,836,245	A→G	V394A (GTG→GCG)	hypF ←	carbamoyltransferase HypF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,836,245	0	A	G	100.0%	163.7 / NA	45	V394A (GTG→GCG)	hypF	carbamoyltransferase HypF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/24);  total (21/24)

ATCAGGCGTAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCACAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCACGATGTCGCGGTTATG  >  NC_000913/2836113‑2836375                                                                                                                                     |                                                                                                                                   aTCAGGCGTAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCgg                                                                                                                             <  2:758234/140‑1 (MQ=255) aTCAGGCGTAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCgg                                                                                                                             <  1:321767/140‑1 (MQ=255)     ggCGTAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACa                                                                                                                         >  2:177187/1‑140 (MQ=255)                tCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGcc                                                                                                              <  1:172531/140‑1 (MQ=255)                tCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGcc                                                                                                              >  2:786204/1‑140 (MQ=255)                  gcgcCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCg                                                                                                            <  2:612890/140‑1 (MQ=255)                  gcgcCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCg                                                                                                            <  1:1012020/140‑1 (MQ=255)                                ggATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAgc                                                                                              >  2:894745/1‑140 (MQ=255)                                   tGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCa                                                                                           >  2:503513/1‑140 (MQ=255)                                   tGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCa                                                                                           >  1:975750/1‑140 (MQ=255)                                     ccATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATc                                                                                         <  1:138119/140‑1 (MQ=255)                                     ccATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATc                                                                                         <  1:272200/140‑1 (MQ=255)                                     ccATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATc                                                                                         <  2:1037420/140‑1 (MQ=255)                                       aTCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCg                                                                                       <  2:975750/140‑1 (MQ=255)                                           tCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGcac                                                                                   >  1:405741/1‑140 (MQ=255)                                           tCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGcaa                                                                                   >  1:688646/1‑139 (MQ=255)                                               ttAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATAc                                                                               >  1:690645/1‑140 (MQ=255)                                                       cccAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGa                                                                       >  2:1001106/1‑140 (MQ=255)                                                         cAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCg                                                                                       >  2:493810/1‑122 (MQ=255)                                                         cAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCg                                                                                       <  1:493810/122‑1 (MQ=255)                                                              gCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGgcgc                                                                >  1:139746/1‑140 (MQ=255)                                                                          ccGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTcgccg                                                    <  1:786204/140‑1 (MQ=255)                                                                            gCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTcgccg                                                    <  2:833912/138‑1 (MQ=255)                                                                            gCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTcgccg                                                    >  1:833912/1‑138 (MQ=255)                                                                            gCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCt                                                  >  1:486092/1‑140 (MQ=255)                                                                              ttGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCttt                                                >  2:93285/1‑140 (MQ=255)                                                                                  tCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTcgcg                                            <  2:486092/140‑1 (MQ=255)                                                                                  tCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTcgcg                                            <  1:58979/140‑1 (MQ=255)                                                                                  tCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTcgcg                                            <  1:177187/140‑1 (MQ=255)                                                                                    aCCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGca                                          <  1:673533/140‑1 (MQ=255)                                                                                      cgcgCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGcacc                                        >  1:60638/1‑140 (MQ=255)                                                                                       gcgcACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGcacca                                       >  2:134444/1‑140 (MQ=255)                                                                                        cgcACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGcaccac                                      >  2:823150/1‑140 (MQ=255)                                                                                           aCCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATTCCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGCACCACCGa                                   <  1:93285/140‑1 (MQ=255)                                                                                                    aaGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGCACCACCGAATcatccat                          >  2:1038210/1‑140 (MQ=255)                                                                                                    aaGGTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGCACCACCGAATcatccat                          >  1:86585/1‑140 (MQ=255)                                                                                                       gTATTTTTCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGCACCGCCGAATCATCCATCCg                       <  1:134444/140‑1 (MQ=255)                                                                                                            tttCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCa                  <  1:503513/140‑1 (MQ=255)                                                                                                              tCAGATCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCACg                <  2:60638/140‑1 (MQ=255)                                                                                                                  aTCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCa                  <  2:746297/134‑1 (MQ=255)                                                                                                                  aTCCGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCa                  >  1:746297/1‑134 (MQ=255)                                                                                                                    ccGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCACGATGTcg          <  2:690645/140‑1 (MQ=255)                                                                                                                    ccGCGCCGAGACACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCACGATGTcg          <  1:1038210/140‑1 (MQ=255)                                                                                                                       cgcCGAGACACAGCGCAGGCGCAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCACGATGTCGCgg       >  2:752213/1‑140 (MQ=255)                                                                                                                           gagaCACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCACGATGTCGCGGTTAt   <  1:894745/140‑1 (MQ=255)                                                                                                                            agaCACAGCGCAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCACGATGTCGCGGTTATg  >  2:220903/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                   ATCAGGCGTAACGCTTCGCGCCACTGCATCTGGATGCCATCGTCACTTAAATCGCCCAGATGCTGACTCAACACCGCTTGTTCACCGCGCACCAGGCAGAAGGTATTTTTCAGATCCGCGCCGAGACACAGCACAGGCGGAACATTTTTAAAGCCCGGAGGCAAAGCCAGCGCATCCGGCACATACCCCCGCGAACGGCGCAGCATTTCGCCGCTTTCGCGCACCACCGAATCATCCATCCGCTGCACGATGTCGCGGTTATG  >  NC_000913/2836113‑2836375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑