Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,860,176	A→G	A139A (GCA→GCG)	pphB →	phosphoprotein phosphatase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,860,176	0	A	G	100.0%	215.8 / NA	57	A139A (GCA→GCG)	pphB	phosphoprotein phosphatase 2
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/25);  total (32/25)

GCTTGCCAGCGGTGGTGACTGGTTTTTCGATTTAAATGATTCAGAGCAACAAGAGGCAATAGATCTGTTGCTGAAATTCCATCACCTTCCACATATTATTGAAATCACTAACGACAACATAAAATATGCCATCGCACATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTGGCCTGTTGATCGTGTGCAGAAATCGCTTAATGGCGAGTTACAACAAATAAACGGCGCTGATTATTTTATAT  >  NC_000913/2860041‑2860312                                                                                                                                        |                                                                                                                                         gCTTGCCAGCGGTGGTGACTGGTTTTTCGATTTAAATGATTCAGAGCAACAAGAGGCAATAGATCTGTTGCTGAAATTCCATCACCTTCCACATATTATTGAAATCACTAACGACAACATAAAATATGCCATCGCGCATg                                                                                                                                      >  2:448238/1‑140 (MQ=255)     gCCAGCGGTGGTGACTGGTTTTTCGATTTAAATGATTCAGAGCAACAAGAGGCAATAGATCTGTTGCTGAAATTCCATCACCTTCCACATATTATTGAAATCACTAACGACAACATAAAATATGCCATCGCGCATGCAGa                                                                                                                                  >  1:545506/1‑140 (MQ=255)            gtggtgACTGGTTTTTCGATTTAAATGATTCAGAGCAACAAGAGGCAATAGATCTGTTGCTGAAATTCCATCACCTTCCACATATTATTGAAATCACTAACGACAACATAAAATATGCCATCGCGCATGCAGATTATCCg                                                                                                                           >  1:5905/1‑140 (MQ=255)              ggtgACTGGTTTTTCGATTTAAATGATTCAGAGCAACAAGAGGCAATAGATCTGTTGCTGAAATTCCATCACCTTCCACATATTATTGAAATCACTAACGACAACATAAAATATGCCATCGCGCATGCAGATTATCCggg                                                                                                                         <  2:545506/140‑1 (MQ=255)                tgACTGGTTTTTCGATTTAAATGATTCAGAGCAACAAGAGGCAATAGATCTGTTGCTGAAATTCCATCACCTTCCACATATTATTGAAATCACTAACGACAACATAAAATATGCCATCGCGCATGCAGATTATCCGGGGa                                                                                                                       <  2:439946/140‑1 (MQ=255)                      gTTTTTCGATTTAAATGATTCAGAGCAACAAGAGGCAATAGATCTGTTGCTGAAATTCCATCACCTTCCACATATTATTGAAATCACTAACGACAACATAAAATATGCCATCGCGCATGCAGATTATCCGGGGAGTGAat                                                                                                                 <  1:294373/140‑1 (MQ=255)                       tttttCGATTTAAATGATTCAGAGCAACAAGAGGCAATAGATCTGTTGCTGAAATTCCATCACCTTCCACATATTATTGAAATCACTAACGACAACATAAAATATGCCATCGCGCATGCAGATTATCCGGGGAGTGAata                                                                                                                >  2:212744/1‑140 (MQ=255)                                             agCAACAAGAGGCAATAGATCTGTTGCTGAAATTCCATCACCTTCCACATATTATTGAAATCACTAACGACAACATAAAATATGCCATCGCGCATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCg                                                                                          >  1:829979/1‑140 (MQ=255)                                                  caaGAGGCAATAGATCTGTTGCTGAAATTCCATCACCTTCCACATATTATTGAAATCACTAACGACAACATAAAATATGCCATCGCGCATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGagag                                                                                     >  1:829123/1‑140 (MQ=255)                                                  caaGAGGCAATAGATCTGTTGCTGAAATCCCATCACCTTCCACATATTATTGAAATCACTAACGACAACATAAAATATGCCATCGCGCATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGagag                                                                                     >  1:457324/1‑140 (MQ=255)                                                      agGCAATAGATCTGTTGCTGAAATTCCATCACCTTCCACATATTATTGAAATCACTAACGACAACATAAAATATGCCATCGCGCATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGaa                                                                                 <  2:862472/140‑1 (MQ=255)                                                       ggCAATAGATCTGTTGCTGAAATTCCATCACCTTCCACATATTATTGAAATCACTAACGACAACATAAAATATGCCATCGCGCATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGagag                                                                                     <  1:820417/135‑1 (MQ=255)                                                       ggCAATAGATCTGTTGCTGAAATTCCATCACCTTCCACATATTATTGAAATCACTAACGACAACATAAAATATGCCATCGCGCATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGagag                                                                                     >  2:820417/1‑135 (MQ=255)                                                       ggCAATAGATCTGTTGCTGAAATTCCATCACCTTCCACATATTATTGAAATCACTAACGACAACATAAAATATGCCATCGCGCATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAAt                                                                                >  1:991624/1‑140 (MQ=255)                                                           aTAGATCTGTTGCTGAAATTCCATCACCTTCCACATATTATTGAAATCACTAACGACAACATAAAATATGCCATCGCGCATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTAct                                                                            <  2:324039/140‑1 (MQ=255)                                                           aTAGATCTGTTGCTGAAATTCCATCACCTTCCACATATTATTGAAATCACTAACGACAACATAAAATATGCCATCGCGCATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTAct                                                                            <  1:448238/140‑1 (MQ=255)                                                           aTAGATCTGTTGCTGAAATCCCATCACCTTCCACATATTATTGAAATCACTAACGACAACATAAAATATGCCATCGCGCATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTAct                                                                            <  2:457324/140‑1 (MQ=255)                                                             aGATCTGTTGCTGAAATTCCATCACCTTCCACATATTATTGAAATCACTAACGACAACATAAAATATGCCATCGCGCATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTActct                                                                          >  1:3516/1‑140 (MQ=255)                                                             aGATCTGTTGCTGAAATTCCATCACCTTCCACATATTATTGAAATCACTAACGACAACATAAAATATGCCATCGCGCATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTActct                                                                          >  2:643122/1‑140 (MQ=255)                                                                tCTGTTGCTGAAATTCCATCACCTTCCACATATTATTGAAATCACTAACGACAACATAAAATATGCCATCGCGCATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGaa                                                                                 >  2:105473/1‑130 (MQ=255)                                                                tCTGTTGCTGAAATTCCATCACCTTCCACATATTATTGAAATCACTAACGACAACATAAAATATGCCATCGCGCATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGaa                                                                                 <  1:105473/130‑1 (MQ=255)                                                                tCTGTTGCTGAAATTCCATCACCTTCCACATATTATTGAAATCACTAACGACAACATAAAATATGCCATCGCGCATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTGGc                                                                       <  2:3516/140‑1 (MQ=255)                                                                 cTGTTGCTGAAATTCCATCACCTTCCACATATTATTGAAATCACTAACGACAACATAAAATATGCCATCGCGCATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTGGcc                                                                      <  2:611751/140‑1 (MQ=255)                                                                 cTGTTGCTGAAATTCCATCACCTTCCACATATTATTGAAATCACTAACGACAACATAAAATATGCCATCGCGCATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTGGcc                                                                      >  1:640219/1‑140 (MQ=255)                                                                         gAAATTCCATCACCTTCCACATATTATTGAAATCACTAACGACAACATAAAATATGCCATCGCGCATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTGGCCTGTTGaac                                                              <  2:829979/140‑3 (MQ=255)                                                                         gAAATTCCATCACCTTCCACATATTATTGAAATCACTAACGACAACATAAAATATGCCATCGCGCATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTGGCCTGTTGATc                                                              >  1:872865/1‑140 (MQ=255)                                                                              tCCATCACCTTCCACATATTATTGAAATCACTAACGACAACATAAAATATGCCATCGCGCATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTGGCCTGTTGATCGtgtg                                                         >  1:519869/1‑140 (MQ=255)                                                                                            caTATTATTGAAATCACTAACGACAACATAAAATATGCCATCGCGCATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTGGCCTGTTGATCGTGTGCAGAAATCGCTTaa                                           >  2:523878/1‑140 (MQ=255)                                                                                               attattGAAATCACTAACGACAACATAAAATATGCCATCGCGCATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTGGCCTGTTGATCGTGTGCAGAAATCGCTTAATgg                                        <  2:500544/140‑1 (MQ=255)                                                                                                ttattGAAATCACTAACGACAACATAAAATATGCCATCGCGCATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTGGCCTGTTGATCGTGTGCAGAAATCGCTTAATGGc                                       >  1:608711/1‑140 (MQ=255)                                                                                                ttattGAAATCACTAACGACAACATAAAATATGCCATCGCGCATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTGGCCTGTTGATCGTGTGCAGAAATCGCTTAATGGc                                       >  1:1003084/1‑140 (MQ=255)                                                                                                 tattGAAATCACTAACGACAACATAAAATATGCCATCGCGCATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTGGCCTGTTGATCGTGTGCAGAAATCGCTTAATGGCg                                      >  2:267067/1‑140 (MQ=255)                                                                                                 tattGAAATCACTAACGACAACATAAAATATGCCATCGCGCATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTGGCCTGTTGATCGTGTGCAGAAATCGCTTAATGGCg                                      >  1:200559/1‑140 (MQ=255)                                                                                                  attGAAATCACTAACGACAACATAAAATATGCCATCGCGCATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTGGCCTGTTGATCGTGTGCAGAAATCGCTTAATGGCGa                                     >  1:257972/1‑140 (MQ=255)                                                                                                       aaTCACTAACGACAACATAAAATATGCCATCGCGCATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTGGCCTGTTGATCGTGTGCAGAAATCGCTTAATGGCGAGTTac                                <  2:519869/140‑1 (MQ=255)                                                                                                        aTCACTAACGACAACATAAAATATGCCATCGCGCATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTGGCCTGTTGATCGTGTGCAGAAATCGCTTAATGGCGAGTTaca                               >  2:500214/1‑140 (MQ=255)                                                                                                         tCACTAACGACAACATAAGATATGCCATCGCGCATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTGGCCTGTTGATCGTGTGCAGAAATCGCTTAATGGCGAGTTacaa                              >  1:712552/1‑140 (MQ=255)                                                                                                           aCTAACGACAACATAAAATATGCCATCGCGCATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTGGCCTGTTGATCGTGTGCAGAAATCGCTTAATGGCGAGTTacaaca                            >  2:997423/1‑140 (MQ=255)                                                                                                             tAACGACAACATAAAATATGCCATCGCGCATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTGGCCTGTTGATCGTGTGCAGAAATCGCTTAATGGCGAGTTACAACaaa                          >  1:258240/1‑140 (MQ=255)                                                                                                                 gACAACATAAAATATGCCATCGCGCATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTGGCCTGTTGATCGTGTGCAGAAATCGCTTAATGGCGAGTTACAACAAATaaa                      <  2:640219/140‑1 (MQ=255)                                                                                                                   caacaTAAAATATGCCATCGCGCATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTGGCCTGTTGATCGTGTGCAGAAATCGCTTAATGGCGAGTTACAACAAATAAACg                    >  1:838120/1‑140 (MQ=255)                                                                                                                    aacaTAAAATATGCCATCGCGCATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTGGCCTGTTGATCGTGTGCAGAAATCGCTTAATGGCGAGTTACAACAAATAAACgg                   >  2:437645/1‑140 (MQ=255)                                                                                                                    aacaTAAAATATGCCATCGCGCATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTGGCCTGTTGATCGTGTGCAGAAATCGCTTAATGGCGAGTTACAACAAATAAACgg                   <  2:854801/140‑1 (MQ=255)                                                                                                                     acaTAAGATATGCCATCGCGCATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTGGCCTGTTGATCGTGTGCAGAAATCGCTTAATGGCGAGTTACAACAAATAAACGgc                  <  2:712552/140‑1 (MQ=255)                                                                                                                     acaTAAAATATGCCATCGCGCATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTGGCCTGTTGATCGTGTGCAGAAATCGCTTAATGGCGAGTTACAACAAATAAACGgc                  >  2:320258/1‑140 (MQ=255)                                                                                                                     acaTAAAATATGCCATCGCGCATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTGGCCTGTTGATCGTGTGCAGAAATCGCTTAATGGCGAGTTACAACAAATAAACGgc                  <  2:1003084/140‑1 (MQ=255)                                                                                                                       aTAAAATATGCCATCGCGCATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTGGCCTGTTGATCGTGTGCAGAAATCGCTTAATGGCGAGTTACAACAAATaa                       <  1:385223/133‑1 (MQ=255)                                                                                                                       aTAAAATATGCCATCGCGCATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTGGCCTGTTGATCGTGTGCAGAAATCGCTTAATGGCGAGTTACAACAAATaa                       >  2:385223/1‑133 (MQ=255)                                                                                                                       aTAAAATATGCCATCGCGCATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTGGCCTGTTGATCGTGTGCAGAAATCGCTTAATGGCGAGTTACAACAAATAAACGgcgc                >  1:787967/1‑140 (MQ=255)                                                                                                                       aTAAAATATGCCATCGCGCATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTGGCCTGTTGATCGTGTGCAGAAATCGCTTAATGGCGAGTTACAACAAATAAACGgcgc                >  1:685540/1‑140 (MQ=255)                                                                                                                          aaaTATGCCATCGCGCATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTGGCCTGTTGATCGTGTGCAGAAATCGCTTAATGGCGAGTTACAACAAATAAACGGCGCTGa             <  1:807185/140‑1 (MQ=255)                                                                                                                               tGCCATCGCGCATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTGGCCTGTTGATCGTGTGCAGAAATCGCTTAATGGCGAGTTACAACAAATAAACGGCGCTGattatt        <  1:212744/140‑1 (MQ=255)                                                                                                                                gCCATCGCGCATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTGGCCTGTTGATCGTGTGCAGAAATCGCTTAATGGCGAGTTACAACAAATAAACg                    >  2:591867/1‑127 (MQ=255)                                                                                                                                gCCATCGCGCATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTGGCCTGTTGATCGTGTGCAGAAATCGCTTAATGGCGAGTTACAACAAATAAACg                    <  1:591867/127‑1 (MQ=255)                                                                                                                                gCCATCGCGCATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTGGCCTGTTGATCGTGTGCAGAAATCGCTTAATGGCGAGTTACAACAAATAAACGGCGCTGATTAttt       <  2:838120/140‑1 (MQ=255)                                                                                                                                gCCATCGCGCATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTGGCCTGTTGATCGTGTGCAGAAATCGCTTAATGGCGAGTTACAACAAATAAACGGCGCTGATTAttt       <  2:991624/140‑1 (MQ=255)                                                                                                                                 ccATCGCGCATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTGGCCTGTTGATCGTGTGCAGAAATCGCTTAATGGCGAGTTACAACAAATAAACGGCGCTGATTAtttt      <  2:200559/140‑1 (MQ=255)                                                                                                                                     cgcgCATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTGGCCTGTTGATCGTGTGCAGAAATCGCTTAATGGCGAGTTACAACAAATAAACGGCGCTGATTATTTTatat  <  1:267067/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         GCTTGCCAGCGGTGGTGACTGGTTTTTCGATTTAAATGATTCAGAGCAACAAGAGGCAATAGATCTGTTGCTGAAATTCCATCACCTTCCACATATTATTGAAATCACTAACGACAACATAAAATATGCCATCGCACATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTGGCCTGTTGATCGTGTGCAGAAATCGCTTAATGGCGAGTTACAACAAATAAACGGCGCTGATTATTTTATAT  >  NC_000913/2860041‑2860312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑