Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,488,579	(C)7→8	coding (1485/1503 nt)	yjgR ←	DUF853 domain‑containing protein YjgR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,488,572	1	.	C	100.0%	120.3 / NA	34	R498G (AGA→GGA)	yjgR	DUF853 domain‑containing protein YjgR
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (17/17);  total (17/17)

TGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCT‑‑CCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGCACCACACCATCTTTCTTCCCGCCGCGTGGTCCGGTAGTGCCGAACAAAATATCCT  >  NC_000913/4488442‑4488706                                                                                                                                    |                                                                                                                                        tGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCT‑‑cccccccca                                                                                                                               >  2:193460/1‑138 (MQ=255)    tAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCT‑‑ccccccccagca                                                                                                                            <  1:466889/140‑6 (MQ=255)     aGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCTC‑CCCCCCCAGCaa                                                                                                                            >  2:49995/1‑140 (MQ=255)              tgCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCTC‑CCCCCCCAGCAAACTCCCCaa                                                                                                                   <  1:259666/140‑1 (MQ=255)                 ttCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCTC‑CCCCCCCAGCAAACTCCCCAACAt                                                                                                                <  1:193460/140‑1 (MQ=255)                      tCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCTC‑CCCCCCCAGCAAACTCCCCAACATCCcgc                                                                                                           >  1:77883/1‑140 (MQ=255)                                    taatTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCTC‑CCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTc                                                                                             >  2:935852/1‑140 (MQ=255)                                      atTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCTC‑CCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGt                                                                                           <  1:49995/140‑1 (MQ=255)                                                       tGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCTCCCCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAgcggcg                                                                           >  1:843433/1‑140 (MQ=255)                                                       tGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCTC‑CCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGgcgc                                                                          >  1:336579/1‑140 (MQ=255)                                                        gCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCTC‑CCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCt                                                                         <  2:406924/140‑1 (MQ=255)                                                              gggtgggCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCTC‑CCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAgcgg                                                                             >  1:432191/1‑130 (MQ=255)                                                              gggtgggCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCTC‑CCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAgcgg                                                                             <  2:432191/130‑1 (MQ=255)                                                               ggtgggCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCTC‑CCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGcc                                                                  <  1:697866/140‑1 (MQ=255)                                                                gtgggCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCTC‑CCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCa                                                                 <  1:935852/140‑1 (MQ=255)                                                                 tgggCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCTC‑CCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCAt                                                                >  2:379140/1‑140 (MQ=255)                                                                      aTGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCTC‑CCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGttt                                                           >  1:695292/1‑140 (MQ=255)                                                                      aTGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCTC‑CCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGttt                                                           >  1:321411/1‑140 (MQ=255)                                                                        gATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCTC‑CCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTgg                                                                    <  1:48731/129‑1 (MQ=255)                                                                        gATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCTC‑CCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTgg                                                                    >  2:48731/1‑129 (MQ=255)                                                                        gATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCTC‑CCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTgg                                                                    <  1:409854/129‑1 (MQ=255)                                                                        gATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCTC‑CCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTgg                                                                    >  2:409854/1‑129 (MQ=255)                                                                         aTAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCTCCCCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGc                                                         <  2:843433/140‑1 (MQ=255)                                                                            aGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCTC‑CCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGcacca                                                     <  2:77883/140‑1 (MQ=255)                                                                             gACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCTC‑CCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGcaccac                                                    >  2:479043/1‑140 (MQ=255)                                                                             gACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCTC‑CCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGcaccac                                                    >  2:816971/1‑140 (MQ=255)                                                                                gcgcCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCTCCCCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGcaccacac                                                  <  2:9867/140‑1 (MQ=255)                                                                                                  gCAAAGACAAATCTCGCCTGACTTACCTTCTTCTC‑CCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGCACCACACCATCTTTCTTCCCGCcgcg                               <  2:321411/140‑1 (MQ=255)                                                                                                  gCAAAGACAAATCTCGCCTGACTTACCTTCTTCTC‑CCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGCACCACACCATCTTTCTTCCCGCcgcg                               <  1:479043/140‑1 (MQ=255)                                                                                                     aaGACAAATCTCGCCTGACTTACCTTCTTCTC‑CCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGCACCACACCATCTTTCTTCCCGCCGCGTgg                            <  1:379140/140‑1 (MQ=255)                                                                                                             tctcGCCTGACTTACCTTCTTCTC‑CCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGCACCACACCATCTTTCTTCCCGCCGCGTGGTCCGGTAg                    >  1:25399/1‑140 (MQ=255)                                                                                                                  ccTGACTTACCTTCTTCTC‑CCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGCACCACACCATCTTTCTTCCCGCCGCGTGGTCCGGTAGTGCCg               >  1:839995/1‑140 (MQ=255)                                                                                                                   cTGACTTACCTTCTTCTC‑CCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGCACCACACCATCTTTCTTCCCGCCGCGTGGTCCGGTAGTGCCGa              <  2:25399/140‑1 (MQ=255)                                                                                                                    tGACTTACCTTCTTCTC‑CCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGCACCACACCATCTTTCTTCCCGCCGCGTGGTCCGGTAGTGCCg               >  2:831946/1‑138 (MQ=255)                                                                                                                    tGACTTACCTTCTTCTC‑CCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGCACCACACCATCTTTCTTCCCGCCGCGTGGTCCGGTAGTGCCg               <  1:831946/138‑1 (MQ=255)                                                                                                                      aCTTACCTTCTTCTC‑CCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGCACCACACCATCTTTCTTCCCGCCGCGTGGTCCGGTAGTGCCGAACa           >  1:772891/1‑140 (MQ=255)                                                                                                                               cttctc‑CCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGCACCACACCATCTTTCTTCCCGCCGCGTGGTCCGGTAGTGCCGAACAAAATATCCt  >  1:72436/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                        TGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCT‑‑CCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGCACCACACCATCTTTCTTCCCGCCGCGTGGTCCGGTAGTGCCGAACAAAATATCCT  >  NC_000913/4488442‑4488706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑