Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	176,292	(G)5→6	coding (1186/1422 nt)	clcA →	chloride:H(+) antiporter ClcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	176,287	1	.	G	100.0%	55.2 / NA	17	M394R (ATG→AGG)	clcA	chloride:H(+) antiporter ClcA
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (2/15);  total (2/15)

TCTCTTCCGGCGCGCCGGGCGGTATTTTTGCCCCGATGCTGGCGCTGGGTACTGTGCTGGGAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGACGTTTGCTATTGCCGGAAT‑‑GGGGGCATTACTGGCGGCATCTATTCGCGCGCCGTTAACGGGGATCATTCTGGTTCTGGAGATGACCGATAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGC  >  NC_000913/176149‑176399                                                                                                                                            |                                                                                                                  tctcTTCCTGCGCGCCGGGCGGTATTTTTGCCCCGATGCTGGCGCTGGGTACTGTGCTGGGAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGACGTTTGCTATTGCCGGAAT‑‑g                                                                                                                 <  2:216630/140‑1 (MQ=255)   tctTCCGGCGCGCCGGGCGGTATTTTTGCCCCGATGCTGGCGCTGGGTACTGTGCTGGGAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGACGTTTGCTATTGCCGGAAT‑‑ggg                                                                                                               <  2:20961/140‑1 (MQ=255)             cgcCGGGCGGTATTTTTGCCCCGATGCTGGCGCTGGGTACTGTGCTGGGAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGACGTTTGCTATTGCCGGAATG‑GGGGGCATTACt                                                                                                      <  1:638676/140‑1 (MQ=255)               ccGGGCGGTATTTTTGCCCCGATGCTGGCGCTGGGTACTGTGCTGGGAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGACGTTTGCTATTGCCGGAATG‑GGGGGCATTACTgg                                                                                                    <  1:196184/140‑1 (MQ=255)                   caggTATTTTTGCCCCGATGCTGGCGCTGGGTACTGTGCTGGGAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGACGTTTGCTATTGCCGGAATG‑GGGGGCATTACTggcggc                                                                                                <  1:813114/138‑1 (MQ=255)                              gCCCCGATGCTGGCGCTGGGTACTGTGCTGGGAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGACGTTTGCTATTGCCGGAATG‑GGGGGCATTACTGGCGGCATCTATTcgcg                                                                                     <  1:846074/140‑1 (MQ=255)                                   gATGCTGGCGCTGGGTACTGTGCTGGGAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGACGTTTGCTATTGCCGGAATG‑GGGGGCATTACTGGCGGCATCTATTCGCGCGCCg                                                                                <  2:634912/140‑1 (MQ=255)                                           cgcTGGGTACTGTGCTGGGAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGACGTTTGCTATTGCCGGAATG‑GGGGGCATTACTGGCGGCATCTATTCGCGCGCCGTTAACggg                                                                        <  1:145048/140‑1 (MQ=255)                                                    cTGTGCTGGGAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGACGTTTGCTATTGCCGGAATG‑GGGGGCATTACTGGCGGCATCTATTCGCGCGCCGTTAACGGGGATCAttct                                                               <  1:926979/140‑1 (MQ=255)                                                     tgtgCTGGGAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGACGTTTGCTATTGCCGGAATGGGGGGGCATTACTGGCGGCATCTATTCGCGCGCCGTTAACGGGGATCAttct                                                               <  2:785482/140‑1 (MQ=255)                                                     tgtgCTGGGAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGACGTTTGCTATTGCCGGAATGGGGGGGCATTACTGGCGGCATCTATTCGCGCGCCGTTAACGGGGATCAttct                                                               <  2:410224/140‑1 (MQ=255)                                                            ggAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGGCGTTTGCTATTTCCGGGATG‑GGGGGGATTACTGGCGGGGTCTATTTGCGCGCCGTTTAAGGGGGGCAtttttgttctgg                                                       >  2:926979/1‑140 (MQ=255)                                                            ggAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGACGTTTGCTATTGCCGGAATG‑GGGGGCATTACTGGCGGCATCTATTCGCGCGCCGTTAACGGGGATCAttctggttctgg                                                       >  2:376513/1‑140 (MQ=255)                                                                 cGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGGGGGGGCGTTTGCTATTGCCGGAATG‑GGGGGCATTACTGGCGGCATCTATTCGCGCGCCGTTAACGGGGATCATTCTGGTTCTGGAGATg                                                  >  2:96544/1‑140 (MQ=255)                                                                 cGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGGCGTTTGGTATTTCCGGGATG‑GGGGGGATTACTGGCGGGATCTATTTCCGCGCCCTTTACGGGGGTCATTCTTGTTCTGGGGATg                                                  >  2:274964/1‑140 (MQ=255)                                                                   cTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGACGTTTGCTATTGCCGGAATG‑GGGGGCATTACTGGCGGCATCTATTCGCGCGCCGTTAACGGGGATCATTCTGGTTCTGGAGATGAc                                                <  2:111597/140‑1 (MQ=255)                                                                   cTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGACGTTTGCTATTGCCGGAATG‑GGGGGCATTACTGGCGGCATCTATTCGCGCGCCGTTAACGGGGATCATTCTGGTTCTGGAGATGAc                                                <  2:163228/140‑1 (MQ=255)                                                                                 gCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGACGTTTGCTATTGCCGGAATG‑GGGGGCATTACTGGCGGCATCTATTCGCGCGCCGTTAACGGGGATCATTCTGGTTCTGGAGATGACCGATAACTACCAGc                                  <  2:19827/140‑1 (MQ=255)                                                                                    gTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGGCGTTTGCTATTGCCGGGATG‑GGGGGGATTACTGGGGGCATCTATTTGCGCGCCGTTAAAGGGGGTCATTTTGGGTCTGGAGATGACCGATAACTACCAGCTCa                               >  2:97864/1‑140 (MQ=255)                                                                                      tGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGACGTTTGCTATTGCCGGAATG‑GGGGGCATTACTGGCGGCATCTATTCGCGCGCCGTTAACGGGGATCATTCTGGTTCTGGAGATGACCGATAACTACCAGCTCAtt                             <  1:274964/140‑1 (MQ=255)                                                                                                       atCACCTTGAGGCGGGGACGTTTGCTATTGCCGGAATG‑GGGGGCATTACTGGCGGCATCTATTCGCGCGCCGTTAACGGGGATCATTCTGGTTCTGGAGATGACCGATAACTACCAGCTCATTTTGCCAATGATTATTAc            <  1:97864/140‑1 (MQ=255)                                                                                                                 gggggggACGTTTGCTATTGCCGGAATG‑GGGGGCATTACTGGCGGCATCTATTCGCGCGCCGTTAACGGGGATCATTCTGGTTCTGGAGATGACCGATAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGgc  <  1:96544/137‑1 (MQ=255)                                                                                                                                            |                                                                                                                  TCTCTTCCGGCGCGCCGGGCGGTATTTTTGCCCCGATGCTGGCGCTGGGTACTGTGCTGGGAACCGCTTTCGGAATGGTTGCCGTTGAGCTGTTTCCGCAATATCACCTTGAGGCGGGGACGTTTGCTATTGCCGGAAT‑‑GGGGGCATTACTGGCGGCATCTATTCGCGCGCCGTTAACGGGGATCATTCTGGTTCTGGAGATGACCGATAACTACCAGCTCATTTTGCCAATGATTATTACCGGTCTTGGC  >  NC_000913/176149‑176399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑