| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,579,871 | (T)8→9 | coding (920/1158 nt) | ydeM ← | putative anaerobic sulfatase maturation enzyme YdeM |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,579,863 | 1 | . | T | 95.3% | 145.9 / ‑0.3 | 43 | coding (928/1158 nt) | ydeM | putative anaerobic sulfatase maturation enzyme YdeM |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base T (18/23); total (19/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.79e-01 | |||||||||||
CCATGGTTGAAAAAAGGATTTTATAACCTTCGCAAAAATAAGAAACAGTCTCATTGTTTACTTTAGTAATACGATGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCG‑TTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTACTGTGGATAGACAAAATGGTCGCATTCGTAAATGTCTCCATTACTTTCAACAACCAGATTAT > NC_000913/1579725‑1579997 | ccATGGTTGAAAAAAGGATTTTATAACCTTCGCAAAAATAAGAAACAGTCTCATTGTTTACTTTAGTAATACGATGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCG‑t > 2:11070/1‑140 (MQ=255) cATGGTTGAAAAAAGGATTTTATAACCTTCGCAAAAATAAGAAACAGTCTCATTGTTTACTTTAGTAATACGATGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCG‑tt > 2:241038/1‑140 (MQ=255) tGGTTGAAAAAAGGATTTTATAACCTTCGCAAAAATAAGAAACAGTCTCATTGTTTACTTTAGTAATACGATGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCG‑tttt > 1:914665/1‑140 (MQ=255) tGAAAAAAGGATTTTATAACCTTCGCAAAAATAAGAAACAGTCTCATTGTTTACTTTAGTAATACGATGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCG‑tttt‑tttt > 2:220946/1‑140 (MQ=255) gAAAAAAGGATTTTATAACCTTCGCAAAAATAAGAAACAGTCTCATTGTTTACTTTAGTAATACGATGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCG‑tttt‑ttttt > 2:464338/1‑139 (MQ=255) gAAAAAAGGATTTTATAACCTTCGCAAAAATAAGAAACAGTCTCATTGTTTACTTTAGTAATACGATGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCG‑tttt‑ttttt > 1:431063/1‑139 (MQ=255) gAAAAAAGGATTTTATAACCTTCGCAAAAATAAGAAACAGTCTCATTGTTTACTCTAGTAATACGATGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCG‑tttt‑ttttt > 1:152802/1‑139 (MQ=255) ggATTTTATACCCTTCGAAAAAATAAAAAACAGTCTCATTTTTTACTTTAGTAATACGATGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGt < 2:172396/140‑1 (MQ=255) ggATTTTATAACCTTCGCAAAAATAAGAAACAGTCTCATTTTTTACTTTAGTAATACGATGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGt < 1:3973/140‑1 (MQ=255) ggATTTTATAACCTTCGCAAAAATAAGAAACAGTCTCATTGTTTACTTTAGTAATACGATGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGt < 1:12765/140‑1 (MQ=255) cccTTCGAAAAAATAAAAAACAGTCTCATTGTTTATTTTAGTAATACGTTGTTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTaca < 2:544438/139‑1 (MQ=255) ccTTCGCAAAAATAAGAAACAGTCTCATTGTTTACTTTAGTAATACGATGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTacac > 1:52016/1‑140 (MQ=255) tCGCAAAAATAAGAAACAGTCTCATTGTTTACTTTAGTAATACGATGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGt > 2:834552/1‑140 (MQ=255) cGCAAAAATAAGAAACAGTCTCATTGTTTACTTTAGTAATACGATGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGtt > 1:436696/1‑140 (MQ=255) cGCAAAAATAAGAAACAGTCTCATTGTTTAC‑TTAGTAATACGATGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCG‑TTTTCTTTTGCGCTGt > 1:521470/1‑124 (MQ=255) cGCAAAAATAAGAAACAGTCTCATTGTTTAC‑TTAGTAATACGATGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCG‑TTTTCTTTTGCGCTGt < 2:521470/124‑1 (MQ=255) aaaaaaCAGTCTCATTGTTTACTTTAGTAATACGATGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGtttt < 2:39982/137‑1 (MQ=255) aCAGTCTTTTTTTTTATTTTAGTAATACGATGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGtt < 2:603981/140‑1 (MQ=255) ttctcTTTGTTTATTTTAGTAATACGATGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAg < 2:152802/139‑1 (MQ=255) gTCTCATTGTTTACTTTAGTAATACGATGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAg > 2:775119/1‑140 (MQ=255) tACTTTAGTAATACGATGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATg > 1:531692/1‑140 (MQ=255) ttttAGTAATACGATGCTTAGAACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGtt < 2:28733/139‑1 (MQ=255) ttAGTAATACGATGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTc < 2:531692/140‑1 (MQ=255) aTGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTAc < 1:775119/140‑1 (MQ=255) aTGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTAc > 2:125476/1‑140 (MQ=255) aTGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGA‑TTATTAATGTTTCCAATTTTGTACt < 1:11070/140‑1 (MQ=255) tGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTACt > 2:934601/1‑140 (MQ=255) tGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTACGCTGTCAGTTGTAAACTGTTCATCGTTTTTGGTTCAGTTTTTTTAATGTTTCAAATTTGGTACt > 2:196780/1‑140 (MQ=255) ccGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTACTGTGGATAGACaa > 1:525824/1‑140 (MQ=255) ccGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTACTGTGGATAGACaa > 2:345280/1‑140 (MQ=255) ccATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTACTGTGGATAGACAAAAt > 2:661304/1‑140 (MQ=255) aTTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTACt > 1:891102/1‑123 (MQ=255) aTTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTACt < 2:891102/123‑1 (MQ=255) gCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTACTGTGGATAGACAAAATGGTCg < 1:14827/140‑1 (MQ=255) cAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTACTGTGGATAGACAAAATGGTCGc < 1:220946/140‑1 (MQ=255) gATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTACTGTGGATAGACAAAATGGTCGCAt < 1:934601/140‑1 (MQ=255) atgTTTATATGCGCCATGCTGACATTACGCTTTTATACGCATAT‑TTTTGCGATTTCAGTTGTATACTTTTCTTCTTTTTCAGTTAAGATTTATTAATGTTTCCAATTTTGTACTGTGTATAGAAAAAATGGCAGTATTCg > 2:433211/3‑140 (MQ=255) aGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTACTGTGGATAGACAAAATGGTCGCATTCg > 2:563892/1‑140 (MQ=255) acaTTGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTACTGTGGATAGACAAAATGGTCGCATTCGTAAATGTCTCCa < 1:345280/140‑1 (MQ=255) tGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTACTGTGGATAGACAAAATGGTCGCATTCGTAAATGTCTCCATTAc > 2:821250/1‑140 (MQ=255) tGCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTACTGTGGATAGACAAAATGGTCGCATTCGTAAATGTCTCCATTAc > 2:153379/1‑140 (MQ=255) gCTGACATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTACTGTGGATAGACAAAATGGTCGCATTCGTAAATGTCTCCATTACt > 2:741224/1‑140 (MQ=255) cATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTACTGTGGATAGACAAAATGGTCGCATTCGTAAATGTCTCCATTACTTTcaa < 1:233426/140‑1 (MQ=255) cATTTCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTACTGTGGATAGACAAAATGGTCGCATTCGTAAATGTCTCCATTACTTTcaa > 1:688407/1‑140 (MQ=255) ttCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTACTGTGGATAGACAAAATGGTCGCATTCGTAAATGTCTCCATTACTTTcaacaa < 1:661304/140‑1 (MQ=255) tCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTACTGTGGATAGACAAAATGGTCGCATTCGTAAATGTCTCCATTACTTTCaacaac > 1:442296/1‑140 (MQ=255) tCGCTGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTACTGTGGATAGACAAAATGGTCGCATTCGTAAATGTCTCCATTACTTTCaacaac < 1:433211/140‑1 (MQ=255) tGGAATCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTACTGTGGATAGACAAAATGGTCGCATTCGTAAATGTCTCCATTACTTTCAACAACCAGa < 1:153379/140‑1 (MQ=255) aaTCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTACTGTGGATAGACAAAATGGTCGCATTCGTAAATGTCTCCATTACTTTCAACAACCAGATTa > 1:919739/1‑140 (MQ=255) aTCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTACTGTGGATAGACAAAATGGTCGCATTCGTAAATGTCTCCATTACTTTCAACAACCAGATTAt < 1:741224/140‑1 (MQ=255) aTCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTACTGTGGATAGACAAAATGGTCGCATTCGTAAATGTCTCCATTACTTTCAACAACCAGATTAt < 1:834552/140‑1 (MQ=255) aTCCGTTTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTACTGTGGATAGACAAAATGGTCGCATTCGTAAATGTCTCCATTACTTTCAACAACCAGATTAt < 2:919739/140‑1 (MQ=255) | CCATGGTTGAAAAAAGGATTTTATAACCTTCGCAAAAATAAGAAACAGTCTCATTGTTTACTTTAGTAATACGATGCTTAGGACAACCGCCATTGCAGATAGGTTTATATGCACATTGCTGACATTTCGCTGGAATCCG‑TTTT‑TTTTGCGCTGTCAGTTGTACACTGTTCATCGTTTTGAGTTCAGATTTATTAATGTTTCCAATTTTGTACTGTGGATAGACAAAATGGTCGCATTCGTAAATGTCTCCATTACTTTCAACAACCAGATTAT > NC_000913/1579725‑1579997 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |