Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,4350CT33.3% 40.6 / 21.7 36intergenic (‑179/‑28)ttdR/ttdADNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (1/23);  new base T (12/0);  total (13/23)
Fisher's exact test for biased strand distribution p-value = 1.04e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01
Rejected as consensus: Frequency below/above cutoff threshold.

AAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGA  >  NC_000913/3206298‑3206566
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aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCa                                                                                                                                    <  1:143473/140‑1 (MQ=255)
  aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGa                                                                                                                                  >  1:760549/1‑140 (MQ=255)
    cATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACa                                                                                                                                <  1:589606/140‑1 (MQ=255)
       tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCTAACaaaa                                                                                                                             >  2:771258/1‑139 (MQ=255)
       tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAAt                                                                                                                             <  1:438524/140‑1 (MQ=255)
          cATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACa                                                                                                                          <  2:202862/140‑1 (MQ=255)
              cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAATAaaaaaa                                                                                                                      >  1:19675/1‑140 (MQ=255)
                 tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTTTGACAaaaaaaaaaaaaa                                                                                                                   >  2:242030/1‑137 (MQ=255)
                   ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGa                                                                                                                 <  1:224670/140‑1 (MQ=255)
                      tGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAAAAAAAAAAATGGA‑ggt                                                                                                             >  2:131954/1‑138 (MQ=255)
                          gcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCa                                                                                                         <  2:441828/140‑1 (MQ=255)
                             atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCatga                                                                                                      <  1:242030/140‑1 (MQ=255)
                                 tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAg                                                                                                  <  1:131954/140‑1 (MQ=255)
                                  cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGc                                                                                                 <  2:760549/140‑1 (MQ=255)
                                     tcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAAAAAAAAAAAAGGA‑GGTGTCCTGATGAGCGaa                                                                                              >  1:923519/1‑140 (MQ=255)
                                        tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCTGACAAAAACAAAAAATGGA‑GTTGCCATGATGATCGaaaaa                                                                                           >  2:485964/1‑138 (MQ=255)
                                          ttttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAAAAAAAAATGGA‑GGTGGCATGATGAGGGGAAGtaa                                                                                         >  2:618792/1‑140 (MQ=255)
                                           tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGtaat                                                                                        <  1:618792/140‑1 (MQ=255)
                                                 gTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCaa                                                                                  <  1:128481/140‑1 (MQ=255)
                                                   tGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACa                                                                                <  1:667874/140‑1 (MQ=255)
                                                     aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGATCGAAAGTAATAAGCAACAgg                                                                              <  1:485964/140‑1 (MQ=255)
                                                          cGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTg                                                                         <  2:59353/140‑1 (MQ=255)
                                                               tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAAAAAAAATGGA‑GTTGCCATGATGAGCGAAAGTAAAAAAAAAAAAGCAGGGAATaa                                                                    >  1:821873/1‑140 (MQ=255)
                                                               tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAAAAAAAAAAAAGGAGGTTGCC‑TGATGAGCGAAAAGAATAAAAAAAAAGCAGGGAaaaa                                                                    >  2:810012/1‑140 (MQ=255)
                                                                 ttAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAAAAAAAAAAATGGA‑GGTGCCATGATGAGGGAAAGTAATAAACAACAAGCAGGGAATaaat                                                                  >  1:272972/1‑138 (MQ=255)
                                                                          tatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAgaga                                                         <  1:35818/140‑1 (MQ=255)
                                                                           atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGAATTTTTCAGACAAAAAAAAAAAATGGA‑GGTGGCATGATGAGGGAAAATAATAAAAAACAAGCAGGGAATAAGTTGACAgagaa                                                        >  2:169563/1‑139 (MQ=255)
                                                                                  gTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTAGACAAAAAAAAAAAATGGA‑GTTTCCATGGTGAGGGAAAAGAAAAAAAAAAAGGCAGAGAATAAAATGAAAGAGATTGttggt                                                 >  2:35818/1‑136 (MQ=255)
                                                                                  gTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCt                                                 <  1:810012/140‑1 (MQ=255)
                                                                                       caaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTAAACAAAAAAAAAAAATGGA‑GGTGCCATGATGAGCGAAAGAAATAAACAACAGGCAGGGAATAAATTGACAAAGATTGTTGCTAACtt                                            >  2:768368/2‑140 (MQ=255)
                                                                                                           tttAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAACCTGGA‑GTTGCCATGATGAGCGAAAGTAAAAAGCAACAGGCAGTGAATAAGTTGAAAGAGATTGTCGCTAACTTTACCGCCATGATTTCTAccc                        <  1:28409/140‑1 (MQ=255)
                                                                                                             tAGTGGTATTTTGTGGCGGTGATTTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAATTTTACCGCCATGATTTCTACCCGa                      <  2:272972/140‑1 (MQ=255)
                                                                                                               gTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAAt                    <  1:122167/140‑1 (MQ=255)
                                                                                                               gTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACGGGA‑GTTGCAATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTATCTTTACCGCCATGATTTCTACCCGAAt                    <  2:113409/140‑1 (MQ=255)
                                                                                                                  gTAGTTTGTGCGGGCAATTTTTTCCAGACAAATAAAAAAACTGGA‑GTTGCAATGAGGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGcc                 <  2:821873/140‑1 (MQ=255)
                                                                                                                    aGTTTGTGGCGGTGGATTTTTTCAGACAAAAACAAAAAATGGA‑GGTGGCATGATGAGCGGAAGTAATAAAAAAAAGGCAGTGAAAAAATTGACAGAGAATGTCGCTAACTTTACCGCCATGATTTTTACCCCAAAGCCtg               >  2:560078/1‑140 (MQ=255)
                                                                                                                          tgGCGGTGAAATTTTTCACACAAAAAAAAAAAAAGGA‑GTTGGCCTTTTGGGGGGAAGAAAAAAAAAAAAAGCAGGGAATAAAGTGACAGAGATTGTCGCTAACTTTTCCCCCATGATTTTTTCCCCAATGCCtgatggag         >  2:224127/1‑137 (MQ=255)
                                                                                                                            gCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtg       <  2:337790/140‑1 (MQ=255)
                                                                                                                            gCGGTGAATTTTTCCAGACAAATACAAAAACGGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtg       <  1:169563/140‑1 (MQ=255)
                                                                                                                            gCGGTGAATTTTTCCAGAAAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtg       <  1:560078/140‑1 (MQ=255)
                                                                                                                                 aaaTTTTTCCAGAAAAATAAAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTATCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa  <  2:557750/139‑1 (MQ=255)
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AAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGA  >  NC_000913/3206298‑3206566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑