Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,885,556	T→C	F161L (TTT→CTT)	yidC →	membrane protein insertase YidC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,885,556	0	T	C	100.0%	222.2 / NA	62	F161L (TTT→CTT)	yidC	membrane protein insertase YidC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/28);  total (34/28)

TCGTGATGGCCCGGATAACCCGGCTAACGGCCCGCGTCCGCTGTATAACGTTGAAAAAGACGCTTATGTGCTGGCTGAAGGTCAAAACGAACTGCAGGTGCCGATGACGTATACCGACGCGGCAGGCAACACGTTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAACGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCT  >  NC_000913/3885423‑3885692                                                                                                                                      |                                                                                                                                         tCGTGATGGCCCGGATAACCCGGCTAACGGCCCGCGTCCGCTGTATAACGTTGAAAAAGACGCTTATGTGCTGGCTGAAGGTCAAAACGAACTGCAGGTGCCGATGACGTATACCGACGCGGCAGGCAACACGCTTACCa                                                                                                                                    <  2:372148/140‑1 (MQ=255)      aTGGCCCGGATAACCCGGCTAACGGCCCGCGTCCGCTGTATAACGTTGAAAAAGACGCTTATGTGCTGGCTGAAGGTCAAAACGAACTGCAGGTGCCGATGACGTATACCGACGCGGCAGGCAACACGCTTACCAAAACg                                                                                                                               >  2:813954/1‑140 (MQ=255)           ccGGATAACCCGGCTAACGGCCCGCGTCCGCTGTATAACGTTGAAAAAGACGCTTATGTGCTGGCTGAAGGTCAAAACGAACTGCAGGTGCCGATGACGTATACCGACGCGGCAGGCAACACGCTTACCaa                                                                                                                                   >  2:419918/1‑131 (MQ=255)           ccGGATAACCCGGCTAACGGCCCGCGTCCGCTGTATAACGTTGAAAAAGACGCTTATGTGCTGGCTGAAGGTCAAAACGAACTGCAGGTGCCGATGACGTATACCGACGCGGCAGGCAACACGCTTACCaa                                                                                                                                   <  1:419918/131‑1 (MQ=255)             ggATAACCCGGCTAACGGCCCGCGTCCGCTGTATAACGTTGAAAAAGACGCTTATGTGCTGGCTGAAGGTCAAAACGAACTGCAGGTGCCGATGACGTATACCGACGCGGCAGGCAACACGCTTACCAAAACGTTTGTcc                                                                                                                        >  1:393839/1‑140 (MQ=255)              gATAACCCGGCTAACGGCCAGCGTCCGCTGTATAACGTTGAAAAAGACGCTTATGTGCTGGCTGAAGGTCAAAACGAACTGCAGGTGCCGATGACGTATACCGACGCGGCAGGCAACACGCTTACCAAAACGTTTGTCCt                                                                                                                       <  1:943668/140‑1 (MQ=255)                    ccGGCTAACGGCCCGCGTCCGCTGTATAACGTTGAAAAAGACGCTTATGTGCTGGCTGAAGGTCAAAACGAACTGCAGGTGCCGATGACGTATACCGACGCGGCAGGCAACACGCTTACCAAAACGTTTGTCCTGAAACg                                                                                                                 <  1:12686/140‑1 (MQ=255)                             ggCCCGCGTCCGCTGTATAACGTTGAAAAAGACGCTTATGTGCTGGCTGAAGGTCAAAACGAACTGCAGGTGCCGATGACGTATACCGACGCGGCAGGCAACACGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTa                                                                                                        >  1:512376/1‑140 (MQ=255)                             ggCCCGCGTCCGCTGTATAACGTTGAAAAAGACGCTTATGTGCTGGCTGAAGGTCAAAACGAACTGCAGGTGCCGATGACGTATACCGACGCGGCAGGCAACACGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTa                                                                                                        >  2:34606/1‑140 (MQ=255)                               cccGCGTCCGCTGTATAACGTTGAAAAAGACGCTTATGTGCTGGCTGAAGGTCAAAACGAACTGCAGGTGCCGATGACGTATACCGACGCGGCAGGCAACACGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACg                                                                                                      <  1:227355/140‑1 (MQ=255)                                  gcgTCCGCTGTATAACGTTGAAAAAGACGCTTATGTGCTGGCTGAAGGTCAAAACGAACTGCAGGTGCCGATGACGTATACCGACGCGGCAGGCAACACGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTg                                                                                                   <  1:372687/140‑1 (MQ=255)                                  gcgTCCGCTGTATAACGTTGAAAAAGACGCTTATGTGCTGGCTGAAGGTCAAAACGAACTGCAGGTGCCGATGACGTATACCGACGCGGCAGGCAACACGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTg                                                                                                   >  1:852169/1‑140 (MQ=255)                                     tCCGCTGTATAACGTTGAAAAAGACGCTTATGTGCTGGCTGAAGGTCAAAACGAACTGCAGGTGCCGATGACGTATACCGACGCGGCAGGCAACACGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTgtca                                                                                                <  1:813954/140‑1 (MQ=255)                                       cGCTGTATAACGTTGAAAAAGACGCTTATGTGCTGGCTGAAGGTCAAAACGAACTGCAGGTGCCGATGACGTATACCGACGCGGCAGGCAACACGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTgtcaac                                                                                              <  1:450055/140‑1 (MQ=255)                                       cGCTGTATAACGTTGAAAAAGACGCTTATGTGCTGGCTGAAGGTCAAAACGAACTGCAGGTGCCGATGACGTATACCGACGCGGCAGGCAACACGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTgtcaac                                                                                              <  2:539146/140‑1 (MQ=255)                                       cGCTGTATAACGTTGAAAAAGACGCTTATGTGCTGGCTGAAGGTCAAAACGAACTGCAGGTGCCGATGACGTATACCGACGCGGCAGGCAACACGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTgtcaac                                                                                              <  1:919001/140‑1 (MQ=255)                                         cTGTATAACGTTGAAAAAGACGCTTATGTGCTGGCTGAAGGTCAAAACGAACTGCAGGTGCCGATGACGTATACCGACGCGGCAGGCAACACGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTgtcaacgt                                                                                            <  1:595839/140‑1 (MQ=255)                                            tataACGTTGAAAAAGACGCTTATGTGCTGGCTGAAGGTCAAAACGAACTGCAGGTGCCGATGACGTATACCGACGCGGCAGGCAACACGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTgtcaacgtcaa                                                                                         <  1:34606/140‑1 (MQ=255)                                              taACGTTGAAAAAGACGCTTATGTGCTGGCTGAAGGTCAAAACGAACTGCAGGTGCCGATGACGTATACCGACGCGGCAGGCAACACGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTgtca                                                                                                >  2:640872/1‑131 (MQ=255)                                              taACGTTGAAAAAGACGCTTATGTGCTGGCTGAAGGTCAAAACGAACTGCAGGTGCCGATGACGTATACCGACGCGGCAGGCAACACGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTgtca                                                                                                <  1:640872/131‑1 (MQ=255)                                                 cGTTGAAAAAGACGCTTATGTGCTGGCTGAAGGTCAAAACGAACTGCAGGTGCCGATGACGTATACCGACGCGGCAGGCAACACGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTgtcaacgtc                                                                                           <  2:937785/133‑1 (MQ=255)                                                 cGTTGAAAAAGACGCTTATGTGCTGGCTGAAGGTCAAAACGAACTGCAGGTGCCGATGACGTATACCGACGCGGCAGGCAACACGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTgtcaacgtc                                                                                           >  1:937785/1‑133 (MQ=255)                                                 cGTTGAAAAAGACGCTTATGTGCTGGCTGAAGGTCAAAACGAACTGCAGGTGCCGATGACGTATACCGACGCGGCAGGCAACACGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACa                                                                                    <  1:683881/140‑1 (MQ=255)                                                  gTTGAAAAAGACGCTTATGTGCTGGCTGAAGGTCAAAACGAACTGCAGGTGCCGATGACGTATACCGACGCGGCAGGCAACACGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACaa                                                                                   >  2:523548/1‑140 (MQ=255)                                                    tGAAAAAGACGCTTATGTGCTGGCTGAAGGTCAAAACGAACTGCAGGTGCCGATGACGTATACCGACGCGGCAGGCAACACGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACg                                                                                 >  1:206635/1‑140 (MQ=255)                                                    tGAAAAAGACGCTTATGTGCTGGCTGAAGGTCAAAACGAACTGCAGGTGCCGATGACGTATACCGACGCGGCAGGCAACACGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACg                                                                                 >  1:718418/1‑140 (MQ=255)                                                      aaaaaGACGCTTATGTGCTGGCTGAAGGTCAAAACGAACTGCAGGTGCCGATGACGTATACCGACGCGGCAGGCAACACGCTTACCAAAACGTTTGTCCTGAACCGTGGTGATTACGCTGTCAACGTCAACTACAACGTg                                                                               >  2:306010/1‑140 (MQ=255)                                                      aaaaaGACGCTTATGTGCTGGCTGAAGGTCAAAACGAACTGCAGGTGCCGATGACGTATACCGACGCGGCAGGCAACACGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTg                                                                               >  2:619951/1‑140 (MQ=255)                                                      aaaaaGACGCTTATGTGCTGGCTGAAGGTCAAAACGAACTGCAGGTGCCGATGACGTATACCGACGCGGCAGGCAACACGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTg                                                                               >  1:825861/1‑140 (MQ=255)                                                      aaaaaGACGCTTATGTGCTGGCTGAAGGTCAAAACGAACTGCAGGTGCCGATGACGTATACCGACGCGGCAGGCAACACGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTg                                                                               >  2:790955/1‑140 (MQ=255)                                                        aaaGACGCTTATGTGCTGGCTGAAGGTCAAAACGAACTGCAGGTGCCGATGACGTATACCGACGCGGCAGGCAACACGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCa                                                                             <  1:619951/140‑1 (MQ=255)                                                            aCGCTTATGTGCTGGCTGAAGGTCAAAACGAACTGCAGGTGCCGATGACGTATACCGACGCGGCAGGCAACACGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAAc                                                                         >  2:775345/1‑140 (MQ=255)                                                                       ctggctgAAGGTCAAAACGAACTGCAGGTGCCGATGACGTATACCGACGCGGCAGGCAACACGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAACGCTGGCGAGaa                                                              >  1:884546/1‑140 (MQ=255)                                                                          gctgAAGGTCAAAACGAACTGCAGGTGCCGATGACGTATACCGACGCGGCAGGCAACACGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAACGCTGGCGAGAAAcc                                                           >  1:848430/1‑140 (MQ=255)                                                                          gctgAAGGTCAAAACGAACTGCAGGTGCCGATGACGTATACCGACGCGGCAGGCAACACGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAACGCTGGCGAGAAAcc                                                           >  1:831627/1‑140 (MQ=255)                                                                                 gTCAAAACGAACTGCAGGTGCCGATGACGTATACCGACGCGGCAGGCAACACGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAACGCTGGCGAGAAACCGCTGGaa                                                    <  2:718418/140‑1 (MQ=255)                                                                                           aCTGCAGGTGCCGATGACGTATACCGACGCGGCAGGCAACACGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAACGCTGGCGAGAAACCGCTGGAAATCTCCTCGt                                          <  2:206635/140‑1 (MQ=255)                                                                                                 ggTGCCGATGACGTATACCGACGCGGCAGGCAACACGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAACGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTgg                                      >  1:377468/1‑138 (MQ=255)                                                                                                 ggTGCCGATGACGTATACCGACGCGGCAGGCAACACGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAACGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTgg                                      <  2:377468/138‑1 (MQ=255)                                                                                                 ggTGCCGATGACGTATACCGACGCGGCAGGCAACACGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAACGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTc                                    >  1:845368/1‑140 (MQ=255)                                                                                                  gTGCCGATGACGTATACCGACGCGGCAGGCAACACGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAACGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGt                                     <  2:902203/138‑1 (MQ=255)                                                                                                  gTGCCGATGACGTATACCGACGCGGCAGGCAACACGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAACGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGt                                     >  1:902203/1‑138 (MQ=255)                                                                                                       gATGACGTATACCGACGCGGCAGGCAACACGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAACGCTGGCGAGAAACCACTGGAAATCTCCTCGTTTGGTCAGTTGa                              >  2:84840/1‑140 (MQ=255)                                                                                                         tGACGTATACCGACGCGGCAGGCAACACGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAACGCTGGCGAGAAACCACTGGAAATCTCCTCGTTTGGTCAGTTGAAg                            <  1:84840/140‑1 (MQ=255)                                                                                                                taCCGACGCGGCAGGCAACACGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAACGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCa                     <  2:831627/140‑1 (MQ=255)                                                                                                                taCCGACGCGGCAGGCAACACGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAACGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCa                     >  2:746741/1‑140 (MQ=255)                                                                                                                 aCCGACGCGGCAGGCAACACGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAACGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCAt                    <  2:512376/140‑1 (MQ=255)                                                                                                                    gACGCGGCAGGCAACACGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAACGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCATCAc                 >  1:664209/1‑140 (MQ=255)                                                                                                                    gACGCGGCAGGCAACACGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAACGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCATCAc                 >  2:458996/1‑140 (MQ=255)                                                                                                                     aCGCGGCAGGCAACACGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAACGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCATCAct                <  1:458996/140‑1 (MQ=255)                                                                                                                     aCGCGGCAGGCAACACGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAACGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCATCAct                >  2:413911/1‑140 (MQ=255)                                                                                                                     aCGCGGCAGGCAACACGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAACGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCATCAct                >  2:145047/1‑140 (MQ=255)                                                                                                                     aCGCGGCAGGCAACACGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAACGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCATCAct                <  2:852169/140‑1 (MQ=255)                                                                                                                        cgGCAGGCAACACGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAACGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCATCACTCTg             >  1:4260/1‑140 (MQ=255)                                                                                                                        cgGCAGGCAACACGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAACGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCATCACTCTg             >  1:724998/1‑140 (MQ=255)                                                                                                                        cgGCAGGCAACACGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAACGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCATCACTCTg             >  1:729777/1‑140 (MQ=255)                                                                                                                        cgGCAGGCAACACGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAACGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCATCACTCTg             >  1:37666/1‑140 (MQ=255)                                                                                                                          gcaggcaACACGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAACGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCa                          <  2:77004/125‑1 (MQ=255)                                                                                                                          gcaggcaACACGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAACGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCa                          >  1:77004/1‑125 (MQ=255)                                                                                                                          gcaggcaACACGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAACGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCATCACTCTGcc           >  2:278303/1‑140 (MQ=255)                                                                                                                                   acGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAACGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCAtct  <  1:746741/140‑1 (MQ=255)                                                                                                                                   acGCTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAACGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCAtct  <  2:884546/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         TCGTGATGGCCCGGATAACCCGGCTAACGGCCCGCGTCCGCTGTATAACGTTGAAAAAGACGCTTATGTGCTGGCTGAAGGTCAAAACGAACTGCAGGTGCCGATGACGTATACCGACGCGGCAGGCAACACGTTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAACGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCT  >  NC_000913/3885423‑3885692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑