Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,927,933	C→T	T260M (ACG→ATG)	asnA →	asparagine synthetase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,927,933	0	C	T	100.0%	205.7 / NA	58	T260M (ACG→ATG)	asnA	asparagine synthetase A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/27);  total (31/27)

GCACCGGATTATGATGACTGGAGCACCCCGTCAGAGCTGGGCCATGCGGGTCTGAACGGCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCT  >  NC_000913/3927797‑3928068                                                                                                                                         |                                                                                                                                        gcACCGGATTATGATGACTGGAGCACCCCGTCAGAGCTGGGCCATGCGGGTCTGAACGGCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACATGCt                                                                                                                                      <  2:165108/140‑1 (MQ=255) gcACCGGATTATGATGACTGGAGCACCCCGTCAGAGCTGGGCCATGCGGGTCTGAACGGCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACATGCt                                                                                                                                      <  2:148123/140‑1 (MQ=255)    ccGGATTATGATGACTGGAGCACCCCGTCAGAGCTGGGCCATGCGGGTCTGAACGGCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACATGCTGaa                                                                                                                                   >  2:370525/1‑140 (MQ=255)      ggATTATGATGACTGGAGCACCCCGTCAGAGCTGGGCCATGCGGGTCTGAACGGCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACATGCTGAAGc                                                                                                                                 <  2:280619/140‑1 (MQ=255)                 aCTGGAGCACCCCGTCAGAGCTGGGCCATGCGGGTCTGAACGGCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACATGCTGAAGCATCAACTGgcg                                                                                                                      >  2:322850/1‑140 (MQ=255)                   tGGAGCACCCCGTCAGAGCTGGGCCATGCGGGTCTGAACGGCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACATGCTGAAGCATCAACTGGCGCt                                                                                                                    >  2:336951/1‑140 (MQ=255)                      aGCACCCCGTCAGAGCTGGGCCATGCGGGTCTGAACGGCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACATGCTGAAGCATCAACTGGCGCTGAc                                                                                                                 >  2:910594/1‑140 (MQ=255)                      aGCACCCCGTCAGAGCTGGGCCATGCGGGTCTGAACGGCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACATGCTGAAGCATCAACTGGCGCTGAc                                                                                                                 <  2:428330/140‑1 (MQ=255)                        cACCCCGTCAGAGCTGGGCCATGCGGGTCTGAACGGCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACATGCTGAAGCATCAACTGGCGCTGACCg                                                                                                               <  1:370525/140‑1 (MQ=255)                          ccccGTCAGAGCTGGGCCATGCGGGTCTGAACGGCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACATGCTGAAGCATCAACTGGCGCTGACCGGt                                                                                                             >  1:640687/1‑140 (MQ=255)                                  gagCTGGGCCATGCGGGTCTGAACGGCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACATGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGa                                                                                                     >  2:211249/1‑140 (MQ=255)                                       gggCCATGCGGGTCTGAACGGCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACATGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGcc                                                                                                <  1:910594/140‑1 (MQ=255)                                        ggCCATGCGGGTCTGAACGGCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACATGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCct                                                                                               >  1:633708/1‑140 (MQ=255)                                        ggCCATGCGGGTCTGAACGGCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACATGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCct                                                                                               >  1:619193/1‑140 (MQ=255)                                         gCCATGCGGGTCTGAACGGCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACATGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCctg                                                                                              >  2:826537/1‑140 (MQ=255)                                          ccATGCGGGTCTGAACGGCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACATGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCctgg                                                                                             <  2:640687/140‑1 (MQ=255)                                             tGCGGGTCTGAACGGCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACATGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCctggagc                                                                                          <  1:211249/140‑1 (MQ=255)                                             tGCGGGTCTGAACGGCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACATGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCctggagc                                                                                          >  2:678618/1‑140 (MQ=255)                                                  gTCTGAACGGCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACATGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCctggagctggag                                                                                     <  2:545355/140‑1 (MQ=255)                                                   tCTGAACGGCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACATGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGt                                                                                    >  2:226149/1‑140 (MQ=255)                                                   tCTGAACGGCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACATGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGt                                                                                    <  2:48812/140‑1 (MQ=255)                                                    cTGAACGGCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACATGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTg                                                                                   <  2:238072/140‑1 (MQ=255)                                                         cGGCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACATGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATc                                                                              <  1:336951/140‑1 (MQ=255)                                                            cGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACATGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCctggagctgga                                                                                      <  1:496214/129‑1 (MQ=255)                                                            cGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACATGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCctggagctgga                                                                                      >  2:496214/1‑129 (MQ=255)                                                                      gtgtGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACATGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTgcg                                                                 <  1:826537/140‑1 (MQ=255)                                                                           gAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACATGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTg                                                            <  1:783409/140‑1 (MQ=255)                                                                           gAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACATGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTg                                                            >  1:415816/1‑140 (MQ=255)                                                                            aaCCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACATGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGa                                                           >  2:86668/1‑140 (MQ=255)                                                                             aCCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACATGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGaa                                                          <  1:322850/140‑1 (MQ=255)                                                                              cccGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACATGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGaaa                                                         <  1:270014/140‑1 (MQ=255)                                                                                cGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACATGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATg                                                       >  2:906086/1‑140 (MQ=255)                                                                                 ggTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACATGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGc                                                      >  2:464479/1‑140 (MQ=255)                                                                                  gTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACATGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGcc                                                     <  2:619193/140‑1 (MQ=255)                                                                                             tGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACATGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATcg                                          >  1:17950/1‑140 (MQ=255)                                                                                             tGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACATGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATcg                                          >  1:105822/1‑140 (MQ=255)                                                                                             tGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACATGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATcg                                          >  1:217457/1‑140 (MQ=255)                                                                                             tGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACATGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATcg                                          >  1:785558/1‑140 (MQ=255)                                                                                               cGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACATGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATcggc                                        >  1:334955/1‑140 (MQ=255)                                                                                                 tttGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACATGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATcggcgg                                      <  2:785558/140‑1 (MQ=255)                                                                                                    gAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACATGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATcggcggcgg                                   >  2:99516/1‑140 (MQ=255)                                                                                                      gCTTTCCTCCATGGGGATCCGTGTAGATGCCGACATGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTa                                 <  1:678618/140‑1 (MQ=255)                                                                                                          tcctccATGGGGATCCGTGTAGATGCCGACATGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCgg                             >  2:356592/1‑140 (MQ=255)                                                                                                          tcctccATGGGGATCCGTGTAGATGCCGACATGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCgg                             <  2:633708/140‑1 (MQ=255)                                                                                                           cctccATGGGGATCCGTGTAGATGCCGACATGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGc                            <  2:17950/140‑1 (MQ=255)                                                                                                               cATGGGGATCCGTGTAGATGCCGACATGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGt                        <  1:226149/140‑1 (MQ=255)                                                                                                                   gggATCCGTGTAGATGCCGACATGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCg                    <  2:105822/140‑1 (MQ=255)                                                                                                                   gggATCCGTGTAGATGCCGACATGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCg                    <  1:99516/140‑1 (MQ=255)                                                                                                                    ggATCCGTGTAGATGCCGACATGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGt                   >  1:317526/1‑140 (MQ=255)                                                                                                                      aTCCGTGTAGATGCCGACATGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCTTCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCCCGttt                 >  2:213368/1‑140 (MQ=255)                                                                                                                                  gCCGACATGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCTGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTAtgctgct     >  2:863305/1‑140 (MQ=255)                                                                                                                                  gCCGACATGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTAtgctgct     >  1:582028/1‑140 (MQ=255)                                                                                                                                  gCCGACATGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTAtgctgct     >  2:420385/1‑140 (MQ=255)                                                                                                                                  gCCGACATGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTAtgctgct     >  2:183767/1‑140 (MQ=255)                                                                                                                                   ccGACATGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGttt                 <  1:550200/127‑1 (MQ=255)                                                                                                                                   ccGACATGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGttt                 >  2:550200/1‑127 (MQ=255)                                                                                                                                    cGACATGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTAtgctgctgc   <  2:217457/140‑1 (MQ=255)                                                                                                                                     gACATGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGTGGCGGTATCGGCCAGTCTCGTTTGACTAtgctgctgct  >  2:390092/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        GCACCGGATTATGATGACTGGAGCACCCCGTCAGAGCTGGGCCATGCGGGTCTGAACGGCGATATTCTGGTGTGGAACCCGGTACTGGAAGATGCGTTTGAGCTTTCCTCCATGGGGATCCGTGTAGATGCCGACACGCTGAAGCATCAACTGGCGCTGACCGGTGACGAAGATCGCCTGGAGCTGGAGTGGCATCAGGCGCTGCTGCGCGGTGAAATGCCGCAGACCATCGGCGGCGGTATCGGCCAGTCTCGTTTGACTATGCTGCTGCT  >  NC_000913/3927797‑3928068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑