Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,966,860 A→G E148E (GAA→GAG)  rho → transcription termination factor Rho

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,966,8600AG100.0% 197.2 / NA 55E148E (GAA→GAG) rhotranscription termination factor Rho
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/26);  total (29/26)

CGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCACCGCCGAAAGCCGGTAAAACCATGCTGCTGCAGAACATTGC  >  NC_000913/3966727‑3966991
                                                                                                                                     |                                                                                                                                   
cgAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAGCGTGGt                                                                                                                               >  2:496073/1‑140 (MQ=255)
  aaaGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAGCGTGGTaa                                                                                                                             <  1:161892/140‑1 (MQ=255)
             aCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAGCGTGGTAACGGTTCTACTg                                                                                                                  >  1:54827/1‑140 (MQ=255)
                       gcgcTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAGCGTGGTAACGGTTCTACTGAAGATTTAAc                                                                                                        <  2:593587/140‑1 (MQ=255)
                        cgcTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAGCGTGGTAACGGTTCTACTGAAGATTTAACt                                                                                                       >  1:548603/1‑140 (MQ=255)
                           tgctgAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAGCGTGGTAACGGTTCTACTGAAGATTTAACTGCt                                                                                                    >  1:192233/1‑140 (MQ=255)
                           tgctgAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAGCGTGGTAACGGTTCTACTGAAGATTTAACTGCt                                                                                                    >  1:756294/1‑140 (MQ=255)
                            gctgAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAGCGTGGTAACGGTTCTACTGAAGATTTAACTGCTc                                                                                                   <  2:54827/140‑1 (MQ=255)
                            gctgAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAGCGTGGTAACGGTTCTACTGAAGATTTAACTGCTc                                                                                                   <  2:756294/140‑1 (MQ=255)
                             ctgAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAGCGTGGTCACGGTTCTACTGAAGATTTAACTGCTcg                                                                                                  >  2:792935/1‑140 (MQ=255)
                             ctgAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAGCGTGGTAACGGTTCTACTGAAGATTTAACTGCTcg                                                                                                  >  2:380584/1‑140 (MQ=255)
                                  aGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAGCGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGTAc                                                                                             <  2:192233/140‑1 (MQ=255)
                                  aGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAGCGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGTAc                                                                                             <  2:488672/140‑1 (MQ=255)
                                   gTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAGCGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGTACt                                                                                            <  1:237005/140‑1 (MQ=255)
                                       aCGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAGCGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGTACTGGAt                                                                                        >  2:801655/1‑140 (MQ=255)
                                       aCGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAGCGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGTACTGGAt                                                                                        >  2:588492/1‑140 (MQ=255)
                                       aCGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAGCGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGTACTGGAt                                                                                        <  1:553366/140‑1 (MQ=255)
                                       aCGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAGCGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGTACTGGAt                                                                                        >  2:6252/1‑140 (MQ=255)
                                          aaGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAGCGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGTACTGGATCTg                                                                                     <  2:150383/140‑1 (MQ=255)
                                            gTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAGCGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGTACTGGATCTGGc                                                                                   <  1:167153/140‑1 (MQ=255)
                                            gTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAGCGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGTACTGGATCTGGc                                                                                   >  2:745851/1‑140 (MQ=255)
                                             ttAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAGCGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGTACTGGATCTGGCa                                                                                  <  1:588492/140‑1 (MQ=255)
                                                     gACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAGCGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGTACTGGATCTGGCAt                                                                                 >  2:733170/1‑133 (MQ=255)
                                                     gACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAGCGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGTACTGGATCTGGCAt                                                                                 <  1:733170/133‑1 (MQ=255)
                                                     gACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAGCGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTAt                                                                          <  2:332129/140‑1 (MQ=255)
                                                     gACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAGCGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTAt                                                                          <  1:235982/140‑1 (MQ=255)
                                                              gAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAGCGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTgg                                                                 <  1:745851/140‑1 (MQ=255)
                                                              gAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAGCGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGTACTGGATATGGCATCACCTATCGGTCGTgg                                                                 <  1:801655/140‑1 (MQ=255)
                                                               aaaaCGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAGCGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGt                                                                >  1:246422/1‑140 (MQ=255)
                                                                 aaCGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAGCGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTgg                                                                 >  2:183506/1‑137 (MQ=255)
                                                                 aaCGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAGCGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTgg                                                                 <  1:183506/137‑1 (MQ=255)
                                                                    gCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAGCGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCg                                                                    >  2:568423/1‑131 (MQ=255)
                                                                    gCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAGCGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCg                                                                    <  1:568423/131‑1 (MQ=255)
                                                                     cccGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAGCGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGt                                                          <  1:380584/140‑1 (MQ=255)
                                                                          aacaaAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAGCGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCt                                                     <  1:698829/140‑1 (MQ=255)
                                                                                   ctctTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAGCGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGc                                            >  1:669264/1‑140 (MQ=255)
                                                                                    tctTTGAGAACTTAACCCCGCTGCACGCGAACTCTCGTCTGCGTATGGAGCGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCa                                           >  2:523921/1‑140 (MQ=255)
                                                                                    tctTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAGCGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGt                                                       <  2:613608/128‑1 (MQ=255)
                                                                                    tctTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAGCGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGt                                                       >  1:613608/1‑128 (MQ=255)
                                                                                      tttGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAGCGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCAcc                                         >  1:140736/1‑140 (MQ=255)
                                                                                             aCTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAGCGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGGGTGGTCTGATTGTGGCACCGCCGaaa                                  <  2:140736/140‑1 (MQ=255)
                                                                                                aacccccGCTGCGGGCAAACGCTCGTCTGCGTAGGGAGCGTGGTAACGGTTCTCTTGAAGATTTACCTGCTCGCGTATTGGATCTGGCATCACCTATCGGTCGTGGTCAGCATGGTCTGATTGTGGCCCCGCCGAAAGcc                               <  2:629512/137‑1 (MQ=255)
                                                                                                      cGCTGCACGCAAACTCTCGTCTGCGTATGGAGCGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCACCGCCGAAAGCCGGTaaa                         >  1:610734/1‑140 (MQ=255)
                                                                                                        cTGCACGCAAACTCTCGTCTGCGTATGGAGCGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCACCGCCGAAAGCCGGTAAAAc                       >  1:629512/1‑140 (MQ=255)
                                                                                                        cTGCACGCAAACTCTCGTCTGCGTATGGAGCGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCACCGCCGAAAGCCGGTAAAAc                       >  1:441931/1‑140 (MQ=255)
                                                                                                        cTGCACGCAAACTCTCGTCTGCGTATGGAGCGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCACCGCCGAAAGCCGGTAAAAc                       >  1:136501/1‑140 (MQ=255)
                                                                                                        cTGCACGCAAACTCTCGTCTGCGTATGGAGCGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCACCGCCGAAAGCCGGTAAAAc                       >  2:810569/1‑140 (MQ=255)
                                                                                                         tGCACGCAAACTCTCGTCTGCGTATGGAGCGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCACCGCCGAAAGCCGGTAAAAcc                      >  1:383439/1‑140 (MQ=255)
                                                                                                         tGCACGCAAACTCTCGTCTGCGTATGGAGCGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCACCGCCGAAAGCCGGTAAAAcc                      >  1:169982/1‑140 (MQ=255)
                                                                                                             cGCAAACTCTCGTCTGCGTATGGAGCGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCACCGCCGAAAGCCGGTAAAACCAtgc                  >  1:565684/1‑140 (MQ=255)
                                                                                                               cAAACTCTCGTCTGCGTATGGAGCGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCACCGCCGAAAGCCGGTAAAACCAtgctg                <  2:246422/140‑1 (MQ=255)
                                                                                                               cAAACTCTCGTCTGCGTATGGAGCGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCACCGCCGAAAGCCGGTAAAACCAtgctg                <  1:523921/140‑1 (MQ=255)
                                                                                                                  aCTCTCGTCTGCGTATGGAGCGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCACCGCCGAAAGCCgg                             >  2:212096/1‑124 (MQ=255)
                                                                                                                  aCTCTCGTCTGCGTATGGAGCGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCACCGCCGAAAGCCgg                             <  1:212096/124‑1 (MQ=255)
                                                                                                                    tctcGTCTGCGTATGGAGCGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCACCGCCGAAAGCCGGTAAAACCATGCTGCTGCa           <  2:548603/140‑1 (MQ=255)
                                                                                                                             cGTATGGAGCGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCACCGCCGAAAGCCGGTAAAACCATGCTGCTGCAGAACATTGc  >  2:460621/1‑140 (MQ=255)
                                                                                                                                     |                                                                                                                                   
CGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGTACTGGATCTGGCATCACCTATCGGTCGTGGTCAGCGTGGTCTGATTGTGGCACCGCCGAAAGCCGGTAAAACCATGCTGCTGCAGAACATTGC  >  NC_000913/3966727‑3966991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑