Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,019,249	A→G	N132S (AAC→AGC)	ubiE →	bifunctional 2‑octaprenyl‑6‑methoxy‑1,4‑benzoquinone methylase and S‑adenosylmethionine:2‑DMK methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,019,249	0	A	G	100.0%	192.8 / NA	54	N132S (AAC→AGC)	ubiE	bifunctional 2‑octaprenyl‑6‑methoxy‑1,4‑benzoquinone methylase and S‑adenosylmethionine:2‑DMK methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/28);  total (26/28)

AGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAATGTATCGCGTGCTGAAACCCGGCGGCCGCCTGCTGGTGCTTGAGTTCTCGAAGCCAATTATCGAG  >  NC_000913/4019112‑4019385                                                                                                                                          |                                                                                                                                         agaAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAgca                                                                                                                                        >  1:451828/1‑140 (MQ=255)      cTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAGCACCttt                                                                                                                                   <  2:473784/140‑1 (MQ=255)       tGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAGCACCTTTg                                                                                                                                  >  2:375107/1‑140 (MQ=255)       tGGCAAAGAGGTCCTTGCTGATATCAATGAACCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAGCACCTTTg                                                                                                                                  >  2:411394/1‑140 (MQ=255)        ggCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAGCACCTTTGa                                                                                                                                 <  2:351748/140‑1 (MQ=255)           aaaGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAGCACCTTTGATTg                                                                                                                              >  1:711081/1‑140 (MQ=255)              gTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAGCACCTTTGATTGCAt                                                                                                                           <  2:759294/140‑1 (MQ=255)              gTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAGCACCTTTGATTGCAt                                                                                                                           >  2:382211/1‑140 (MQ=255)                           atatCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAGCACCTTTGATTGCATCACCATTTCGttt                                                                                                              >  1:390855/1‑140 (MQ=255)                           atatCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAGCACCTTTGATTGCATCACCATTTCGttt                                                                                                              >  1:703019/1‑140 (MQ=255)                              tCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAGCACCTTTGATTGCATCACCATTTCGTTTGGt                                                                                                           <  2:118932/140‑1 (MQ=255)                              tCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAGCACCTTTGATTGCATCACCATTTCGTTTGGt                                                                                                           <  2:451828/140‑1 (MQ=255)                                aaTGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAGCACCTTTGATTGCATCACCATTTCGTTTGGTCt                                                                                                         >  1:184679/1‑140 (MQ=255)                                     aTCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAGCACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTa                                                                                                    <  2:390855/140‑1 (MQ=255)                                     aTCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAGCACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTa                                                                                                    >  1:886294/1‑140 (MQ=255)                                      tCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAGCACCTTTGATTGCATCACCATTTCGTTTGGt                                                                                                           <  2:449673/132‑1 (MQ=255)                                      tCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAGCACCTTTGATTGCATCACCATTTCGTTTGGt                                                                                                           >  1:449673/1‑132 (MQ=255)                                          tGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAGCACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTc                                                                                               >  2:26306/1‑140 (MQ=255)                                          tGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAGCACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTc                                                                                               >  1:137232/1‑140 (MQ=255)                                          tGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAGCACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTc                                                                                               >  2:877002/1‑140 (MQ=255)                                                aaaTGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAGCACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGAc                                                                                         <  1:26306/140‑1 (MQ=255)                                                aaaTGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAGCACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGAc                                                                                         <  1:877002/140‑1 (MQ=255)                                                aaaTGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAGCACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGAc                                                                                         >  2:496078/1‑140 (MQ=255)                                                 aaTGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAGCACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACa                                                                                        <  2:703019/140‑1 (MQ=255)                                                  aTGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAGCACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACaa                                                                                       <  1:583750/140‑1 (MQ=255)                                                      gCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAGCACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCa                                                                                              >  2:464376/1‑129 (MQ=255)                                                      gCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAGCACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCa                                                                                              <  1:464376/129‑1 (MQ=255)                                                      gCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAGCACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGAt                                                                                   >  1:143175/1‑140 (MQ=255)                                                          cgAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAGCACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAg                                                                               >  1:382418/1‑140 (MQ=255)                                                             gaAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAGCACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCAc                                                                            <  2:382418/140‑1 (MQ=255)                                                             gaAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAGCACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCAc                                                                            <  1:378744/140‑1 (MQ=255)                                                             gaAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAGCACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCAc                                                                            <  1:382211/140‑1 (MQ=255)                                                              aaGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAGCACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACt                                                                           <  2:760465/140‑1 (MQ=255)                                                                 cTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAGCACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCg                                                                        <  2:137232/140‑1 (MQ=255)                                                                          atCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAGCACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAATGTa                                                               <  2:143175/140‑1 (MQ=255)                                                                            cGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTAAGGCGCTGCCGTTCCCGGATAGCACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAATGTATc                                                             <  1:160805/140‑1 (MQ=255)                                                                                   aTTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAGCACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGt                                                                       >  2:5012/1‑123 (MQ=255)                                                                                   aTTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAGCACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGt                                                                       <  1:5012/123‑1 (MQ=255)                                                                                   aTTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAGCACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAATGTATCGCGTGCt                                                      >  2:468326/1‑140 (MQ=255)                                                                                   aTTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAGCACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAATGTATCGCGTGCt                                                      >  2:157500/1‑140 (MQ=255)                                                                                   aTTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAGCACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAATGTATCGCGTGCt                                                      >  1:640639/1‑140 (MQ=255)                                                                                        cAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAGCACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAATGTATCGCGTGCTGAAAc                                                 <  1:375107/140‑1 (MQ=255)                                                                                         aaCGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAGCACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAATGTATCGCGTGCTGAAAcc                                                >  2:833878/1‑140 (MQ=255)                                                                                             ttGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAGCACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAATGTATCGCGTGCTGAAACCcggc                                            >  1:360612/1‑140 (MQ=255)                                                                                               gAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAGCACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAATGTATCGCGTGCTGAAACCcggcgg                                          <  1:565778/140‑1 (MQ=255)                                                                                                      ttCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAGCACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAATGTATCGCGTGCTGAAACCCGGCGGCCGCctg                                   >  2:730581/1‑140 (MQ=255)                                                                                                       tCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAGCACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAATGTATCGCTTGCTGAAACCCGGCGGCCGCctgc                                  >  1:453989/1‑140 (MQ=255)                                                                                                       tCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAGCACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAATGTATCGCGTGCTGAAACCCGGCGGCCGCctgc                                  <  1:157500/140‑1 (MQ=255)                                                                                                       tCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAGCACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAATGTATCGCGTGCTGAAACCCGGCGGCCGCctgc                                  <  1:833878/140‑1 (MQ=255)                                                                                                           gCGAACGCTGAGGCGCTGCCGTTCCCGGATAGCACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAATGTATCGCGTGCTGAAACCCGGCGGCCGCCTGCTGGt                              >  1:417091/1‑140 (MQ=255)                                                                                                              aaCGCTGAGGCGCTGCCGTTCCCGGATAGCACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAATGTATCGCGTGCTGAAACCCGGCGGCCGCCTGCTGGTGCt                           >  1:142700/1‑140 (MQ=255)                                                                                                                cGCTGTGGCGCTGCCGTTCCCGGATAGCACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAATGTATCGCGTGCTGAAACCCGGCGGCCGCCTGCTGGTGCtt                          <  2:742529/139‑1 (MQ=255)                                                                                                                cGCTGTGGCGCTGCCGTTCCCGGATAGCACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAATGTATCGCGTGCTGAAACCCGGCGGCCGCCTGCTGGTGCtt                          >  1:742529/1‑139 (MQ=255)                                                                                                                             ccGTTCCCGGATAGCACCTTTGATTGCATCACCATTTCGTTTTGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAATGTATCGCGAGCTGAAACCCGGCGGCCGCCTGCTGGTGCTTGAGTTCTCGAAGcc            <  2:886294/140‑1 (MQ=255)                                                                                                                                     ggATAGCACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAATGTATCGCGTGCTGAAACCCGGCGGCCGCCTGCTGGTGCTTGAGTTCTCGAAGCCAATTATCg    <  1:496078/140‑1 (MQ=255)                                                                                                                                       aTAGCACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAATGTATCGCGTGCTGAAACCCGGCGGCCGCCTGCTGGTGCTTGAGTTCTCGAAGCCAATTATCGAg  <  2:360612/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         AGAAACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAATGTATCGCGTGCTGAAACCCGGCGGCCGCCTGCTGGTGCTTGAGTTCTCGAAGCCAATTATCGAG  >  NC_000913/4019112‑4019385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑