Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	334,736	A→G	V96A (GTA→GCA)	yahC ←	uncharacterized protein YahC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	334,736	0	A	G	100.0%	174.4 / NA	49	V96A (GTA→GCA)	yahC	uncharacterized protein YahC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/26);  total (23/26)

CCCGCCAGAAAACCCAGTAACGTCACCGTCCAGTTCATCCCGCTGGCAAAAAACAGCGTCATGCCGAGAAAGCCACCGGGAATAAAGGACAGTAACCAGAAACGCCCTT‑GCCAGACCACTACCGCCGAGAAGGGTACGGTAGTAATTACGCTCGCCCAGAACGCGGACAGTCCACTTACAGAGGCCAGCCAACCAGCAGTCAGCGCGGCGAAAAACGCCCACACCATCCCGGAATAATTAACCGCCAGACTTCTGATAAACCCCGATTTAC  >  NC_000913/334601‑334871                                                                                                                                         |                                                                                                                                        cccGCCAGAAAACCCAGTAACGTCACCGTCCAGTTCATCCCGCTGGCAAAAAACAGCGTCATGCCGAGAAAGCCACCGGGAATAAAGGACAGTAACCAGAAACGCCCTT‑GCCAGACCACTACCGCCGAGAAGGGTGCGgt                                                                                                                                     <  2:103862/140‑1 (MQ=255)      cAGAAAACCCAGTAACGTCACCGTCCAGTTCATCCCGCTGGCAAAAAACAGCGTCATGCCGAGAAAGCCACCGGGAATAAAGGACAGTAACCAGAAACGCCCTT‑GCCAGACCACTACCGCCGAGAAGGGTGCGGTAGTaa                                                                                                                                >  2:297614/1‑140 (MQ=255)      cAGAAAACCCAGTAACGTCACCGTCCAGTTCATCCCGCTGGCAAAAAACAGCGTCATGCCGAGAAAGCCACCGGGAATAAAGGACAGTAACCAGAAACGCCCTT‑GCCAGACCACTACCGCCGAGAAGGGTGCGGTAGTaa                                                                                                                                >  1:353059/1‑140 (MQ=255)           aaCCCAGTAACGTCACCGTCCAGTTCATCCCGCTGGCAAAAAACAGCGTCATGCCGAGAAAGCCACCGGGAATAAAGGACAGTAACCAGAAACGCCCTT‑GCCAGACCACTACCGCCGAGAAGGGTGCGGTAGTAATTACg                                                                                                                           <  2:881820/140‑1 (MQ=255)           aaCCCAGTAACGTCACCGTCCAGTTCATCCCGCTGGCAAAAAACAGCGTCATGCCGAGAAAGCCACCGGGAATAAAGGACAGTAACCAGAAACGCCCTT‑GCCAGACCACTACCGCCGAGAAGGGTGCGGTAGTAATTACg                                                                                                                           <  2:851824/140‑1 (MQ=255)           aaCCCAGTAACGTCACCGTCCAGTTCATCCCGCTGGCAAAAAACAGCGTCATGCCGAGAAAGCCACCGGGAATAAAGGACAGTAACCAGAAACGCCCTT‑GCCAGACCACTACCGCCGAGAAGGGTGCGGTAGTAATTACg                                                                                                                           <  1:465353/140‑1 (MQ=255)             cccAGTAACGTCACCGTCCAGTTCATCCCGCTGGCAAAAAACAGCGTCATGCCGAGAAAGCCACCGGGAATAAAGGACAGTAACCAGAAACGCCCTT‑GCCAGACCACTACCGCCGAGAAGGGTGCGGTAGTAATTACGCt                                                                                                                         >  2:194911/1‑140 (MQ=255)             cccAGTAACGTCACCGTCCAGTTCATCCCGCTGGCAAAAAACAGCGTCATGCCGAGAAAGCCACCGGGAATAAAGGACAGTAACCAGAAACGCCCTT‑GCCAGACCACTACCGCCGAGAAGGGTGCGGTAGTAATTACGCt                                                                                                                         <  2:246130/140‑1 (MQ=255)                 gTAACGTCACCGTCCAGTTCATCCCGCTGGCAAAAAACAGCGTCATGCCGAGAAAGCCACCGGGAATAAAGGACAGTAACCAGAAACGCCCTT‑GCCAGACCACTACCGCCGAGAAGGGTGCGGTAGTAATTACGCTCGcc                                                                                                                     <  2:254181/140‑1 (MQ=255)                  tAACGTCACCGTCCAGTTCATCCCGCTGGCAAAAAACAGCGTCATGCCGAGAAAGCCACCGGGAATAAAGGACAGTAACCAGAAACGCCCTT‑GCCAGACCACTACCGCCGAGAAGGGTGCGGTAGTAATTACGCTCGccc                                                                                                                    <  1:297614/140‑1 (MQ=255)                       tCACCGTCCAGTTCATCCCGCTGGCAAAAAACAGCGTCATGCCGAGAAAGCCACCGGGAATAAAGGACAGTAACCAGAAACGCCCTT‑GCCAGACCACTACCGCCGAGAAGGGTGCGGTAGTAATTACGCTCGCCCAGAAc                                                                                                               <  1:111334/140‑1 (MQ=255)                        cACCGTCCAGTTCATCCCGCTGGCAAAAAACAGCGTCATGCCGAGAAAGCCACCGGGAATAAAGGACAGTAACCAGAAACGCCCTT‑GCCAGACCACTACCGCCGAGAAGGGTGCGGTAGTAATTACGCt                                                                                                                         <  2:847697/129‑1 (MQ=255)                        cACCGTCCAGTTCATCCCGCTGGCAAAAAACAGCGTCATGCCGAGAAAGCCACCGGGAATAAAGGACAGTAACCAGAAACGCCCTT‑GCCAGACCACTACCGCCGAGAAGGGTGCGGTAGTAATTACGCt                                                                                                                         >  1:847697/1‑129 (MQ=255)                             tCCAGTTCATCCCGCTGGAAAAAAACAGCGTCATGCCGAGAAAGCCACCGGGAATAAAGGACAGTAACCAGAAACGCCCTT‑GCCAGACCACTACCGCCGAGAAGGGTGCGGTAGTAATTACGCTCGCCCAGAACGCGGAc                                                                                                         <  2:353059/140‑1 (MQ=255)                             cccAGTCCACCCCGCTGGCAAAAAACAGCGTCATGCCGAGAAAGCCACCGGGAATAAAGGACAGTAACCAGAAACGCCCTT‑GCCAGACCACTACCGCCGAGAAGGGTGCGGTAGTAATTACGCTCGCCCAGAACGCGGAc                                                                                                         <  1:236820/139‑1 (MQ=255)                               cAGTTCATCCCGCTGGCAAAAAACAGCGTCATGCCGAGAAAGCCCCCGGGAATAAAGGACAGTAACCAGAAACGCCCTT‑GCCAGACCACTACCGCCGAGAAGGGTGCGGTAGTAATTACGCTCGCCCAGAACGCGGACAg                                                                                                       <  2:678347/140‑1 (MQ=255)                                aGTTCATCCCGCTGGCAAAAAACAGCGTCATGCCGAGAAAGCCACCGGGAATAAAGGACAGTAACCAGAAACGCCCTT‑GCCAGACCACTACCGCCGAGAAGGGTGCGGTAGTAATTACGCTCGCCCAGAACGCGGACAGt                                                                                                      <  1:728674/140‑1 (MQ=255)                                    cATCCCGCTGGCAAAAAACAGCGTCATGCCGAGAAAGCCACCGGGAATAAAGGACAGTAACCAGAAACGCCCTT‑GCCAGACCACTACCGCCGAGAAGGGTGCGGTAGTAATTACGCTCGCCCAGAACGCGGa                                                                                                          <  2:849862/132‑1 (MQ=255)                                    cATCCCGCTGGCAAAAAACAGCGTCATGCCGAGAAAGCCACCGGGAATAAAGGACAGTAACCAGAAACGCCCTT‑GCCAGACCACTACCGCCGAGAAGGGTGCGGTAGTAATTACGCTCGCCCAGAACGCGGa                                                                                                          >  1:849862/1‑132 (MQ=255)                                       cccGCTGGCAAAAAACAGCGTCATGCCGAGAAAGCCACCGGGAATAAAGGACAGTAACCAGAAACGCCCTT‑GCCAGACCACTACCGCCGAGAAGGGTGCGGTAGTAATTACGCTCGCCCAGAACGCGGACAGTCCACTTa                                                                                               >  1:37749/1‑140 (MQ=255)                                            tGGCAAAAAACAGCGTCATGCCGAGAAAGCCACCGGGAATAAAGGACAGTAACCAGAAACGCCCTT‑GCCAGACCACTACCGCCGAGAAGGGTGCGGTAGTAATTACGCTCGCCCAGAACGCGGACAGTCCACTTACagag                                                                                          >  2:108177/1‑140 (MQ=255)                                                    aaCAGCGTCATGCCGAGAAAGCCACCGGGAATAAAGGACAGTAACCAGAAACGCCCTT‑GCCAGACCACTACCGCCGAGAAGGGTGCGGTAGTAATTACGCTCGCCCAGAACGCGGACAGTCCACTTACAGAGgccagcca                                                                                  >  2:94430/1‑140 (MQ=255)                                                        gCGTCATGCCGAGAAAGCCACCGGGAATAAAGGACAGTAACCAGAAACGCCCTT‑GCCAGACCACTACCGCCGAGAAGGGTGCGGTAGTAATTACGCTCGCCCAGAACGCGGACAGTCCACTTACAGAGGCCAGCCAACca                                                                              >  1:143015/1‑140 (MQ=255)                                                        gCGTCATGCCGAGAAAGCCACCGGGAATAAAGGACAGTAACCAGAAACGCCCTT‑GCCAGACCACTACCGCCGAGAAGGGTGCGGTAGTAATTACGCTCGCCCAGAACGCGGACAGTCCACTTACAGAGGCCAGCCAACca                                                                              >  1:939191/1‑140 (MQ=255)                                                             aTGCCGAGAAAGCCACCGGGAATAAAGGACAGTAACCAGAAACGCCCTT‑GCCAGACCACTACCGCCGAGAAGGGTGCGGTAGTAATTACGCTCGCCCAGAACGCGGACAGTCCACTTACAGAGGCCAGCCAACCAGCAGt                                                                         <  1:523641/140‑1 (MQ=255)                                                                   agaAAGCCACCGGGAATAAAGGACAGTAACCAGAAACGCCCTT‑GCCAGACCACTACCGCCGAGAAGGGTGCGGTAGTAATTACGCTCGCCCAGAACGCGGACAGTCCACTTACAGAGGCCAGCCAACCAGCAGTCAgcgc                                                                   >  2:297984/1‑140 (MQ=255)                                                                          cACCGGGAATAAAGGACAGTAACCAGAAACGCCCTT‑GCCAGACCACTACCGCTGAGAAGGGTGCGGTAGTAATTACGCTCGCCCAGAACGCGGACAGTCCACTTACAGAGGCCAGCCAACCAGCAGTCAGCGCGGCGaaa                                                            <  2:123292/140‑1 (MQ=255)                                                                          cACCGGGAATAAAGGACAGTAACCAGAAACGCCCTT‑GCCAGACCACTACCGCCGAGAAGGGTGCGGTAGTAATTACGCTCGCCCAGAACGCGGACAGTCCACTTACAGAGGCCAGCCAACCAGCAGTCAGCGCGGCGaaa                                                            <  2:651292/140‑1 (MQ=255)                                                                               ggAATAAAGGACAGTAACCAGAAACGCCCTT‑GCCAGACCACTACCGCCGAGAAGGGTGCGGTAGTAATTACGCTCGCCCAGAACGCGGACAGTCCACTTACAGAGGCCAGCCAACCAGCAGTCAGCGCGGCGAAAAACGc                                                       <  1:297984/140‑1 (MQ=255)                                                                                       ggACAGTAACCAGAAACGCCCTT‑GCCAGACCACTACCGCCGAGAAGGGTGCGGTAGTAATTACGCTCGCCCAGAACGCGGACAGTCCACTTACAGAGGCCAGCCAACCAGCAGTCAGCGCGGCGAAAAACGCCCACACCa                                               <  1:94430/140‑1 (MQ=255)                                                                                         aCAGTAACCAGAAACGCCCTT‑GCCAGACCACTACCGCCGAGAAGGGTGCGGTAGTAATTACGCTCGCCCAGAACGCGGACAGTCCACTTACAGAGGCCAGCCAACCAGCAGTCAGCGCGGCGAAAAACGCCCACACCATc                                             >  1:90951/1‑140 (MQ=255)                                                                                           aGTAACCAGAAACGCCCTT‑GCCAGACCACTACCGCCGAGAAGGGTGCGGTAGTAATTACGCTCGCCCAGAACGCGGACAGTCCACTTACAGAGGCCAGCCAACCAGCAGTCAGCGCGGCGAAAAACGCCCACACCATc                                             <  2:238082/138‑1 (MQ=255)                                                                                           aGTAACCAGAAACGCCCTT‑GCCAGACCACTACCGCCGAGAAGGGTGCGGTAGTAATTACGCTCGCCCAGAACGCGGACAGTCCACTTACAGAGGCCAGCCAACCAGCAGTCAGCGCGGCGAAAAACGCCCACACCATc                                             >  1:238082/1‑138 (MQ=255)                                                                                               aCCAGAAACGCCCTT‑GCCAGACCACTACCGCCGAGAAGGGTGCGGTAGTAATTACGCTCGCCCAGAACGCGGACAGTCCACTTACAGAGGCCAGCCAACCAGCAGTCAGCGCGGCGAAAAACGCCCACACCATCCCGGaa                                       >  2:681830/1‑140 (MQ=255)                                                                                                      acccccTTGGCCAGCCCACTACCGCCGAGAAGGGTGCGGTAGTAATTACGCTCGCCCAGAACGCGGACAGTCCACTTACAGAGGCCAGCCAACCAGCAGTCAGCGCGGCGAAAAACGCCCACACCATCCCGGAATAATTa                                 <  2:939191/137‑1 (MQ=255)                                                                                                       cGCCCTT‑GCCAGACCACTACCGCCGAGAAGGGTGCGGTAGTAATTACGCTCGCCCAGAACGCGGACAGTCCACTTACAGAGGCCAGCCAACCAGCAGTCAGCGCGGCGAAAAACGCCCACACCATc                                             <  2:937313/126‑1 (MQ=255)                                                                                                       cGCCCTT‑GCCAGACCACTACCGCCGAGAAGGGTGCGGTAGTAATTACGCTCGCCCAGAACGCGGACAGTCCACTTACAGAGGCCAGCCAACCAGCAGTCAGCGCGGCGAAAAACGCCCACACCATc                                             <  2:346482/126‑1 (MQ=255)                                                                                                       cGCCCTT‑GCCAGACCACTACCGCCGAGAAGGGTGCGGTAGTAATTACGCTCGCCCAGAACGCGGACAGTCCACTTACAGAGGCCAGCCAACCAGCAGTCAGCGCGGCGAAAAACGCCCACACCATc                                             >  1:937313/1‑126 (MQ=255)                                                                                                       cGCCCTT‑GCCAGACCACTACCGCCGAGAAGGGTGCGGTAGTAATTACGCTCGCCCAGAACGCGGACAGTCCACTTACAGAGGCCAGCCAACCAGCAGTCAGCGCGGCGAAAAACGCCCACACCATc                                             >  1:346482/1‑126 (MQ=255)                                                                                                         cccTT‑GCCAGACCACTACCGCCGAGAAGGGTGCGGTAGTAATTACGCTCGCCCAGAACGCGGACAGTCCACTTACAGAGGCCAGCCAACCAGCAGTCAGCGCGGCGAAAAACGCCCACACCATcc                                            >  2:258546/1‑125 (MQ=255)                                                                                                         cccTT‑GCCAGACCACTACCGCCGAGAAGGGTGCGGTAGTAATTACGCTCGCCCAGAACGCGGACAGTCCACTTACAGAGGCCAGCCAACCAGCAGTCAGCGCGGCGAAAAACGCCCACACCATcc                                            <  1:258546/125‑1 (MQ=255)                                                                                                         cccTT‑GCCAGACCACTACCGCCGAGAAGGGTGCGGTAGTAATTACGCTCGCCCAGAACGCGGACAGTCCACTTACAGAGGCCAGCCAACCAGCAGTCAGCGCGGCGAAAAACGCCCACACCATCCCGGAATAATTAACCg                             >  2:435495/1‑140 (MQ=255)                                                                                                            tt‑GCCAGACCACTACCGCCGAGAAGGGTGCGGTAGTAATTACGCTCGCCCAGAACGCGGACAGTCCACTTACAGAGGCCAGCCAACCAGCAGTCAGCGCGGCGAAAAACGCCCACACCATCCCGGAATAATTAACCGCCa                          <  1:194911/140‑1 (MQ=255)                                                                                                            tt‑GCCAGACCACTACCGCCGAGAAGGGTGCGGTAGTAATTACGCTCGCCCAGAACGCGGACAGTCCACTTACAGAGGCCAGCCAACCAGCAGTCAGCGCGGCGAAAAACGCCCACACCATCCCGGAATAATTAACCGCCa                          <  1:435495/140‑1 (MQ=255)                                                                                                                          aCCGCCGAGAAGGGTGCGGTAGTAATTACGCTCGCCCAGAACGCGGACAGTCCACTTACAGAGGCCAGCCAACCAGCAGTCAGCGCGGCGAAAAACGCCCACACCATCCCGGAATAATTAACCGCCAGACTTCTGATaaa             >  2:641006/1‑140 (MQ=255)                                                                                                                          aCCGCCGAGAAGGGTGCGGTAGTAATTACGCTCGCCCAGAACGCGGACAGTCCACTTACAGAGGCCAGCCAACCAGCAGTCAGCGCGGCGAAAAACGCCCACACCATCCCGGAATAATTAACCGCCAGACTTCTGATaaa             >  1:478091/1‑140 (MQ=255)                                                                                                                          aCCGCCGAGAAGGGTGCGGTAGTAATTACGCTCGCCCAGAACGCGGACAGTCCACTTACAGAGGCCAGCCAACCAGCAGTCAGCGCGGCGAAAAACGCCCACACCATCCCGGAATAATTAACCGCCAGACTTCTGATaaa             >  1:63605/1‑140 (MQ=255)                                                                                                                                gagaAGGGTGCGGTAGTAATTACGCTCGCCCAGAACGCGGACAGTCCACTTACAGAGGCCAGCCAACCAGCAGTCAGCGCGGCGAAAAACGCCCACACCATCCCGGAATAATTAACCGCCAGACTTCTGATAAACCCCGa       >  1:85401/1‑140 (MQ=255)                                                                                                                                     gggTGCGGTAGTAATTACGCTCGCCCAGAACGCGGACAGTCCACTTACAGAGGCCAGCCAACCAGCAGTCAGCGCGGCGAAAAACGCCCACACCATCCCGGAATAATTAACCGCCAGACTTCTGATAAACCCCGATTTAc  >  1:777931/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        CCCGCCAGAAAACCCAGTAACGTCACCGTCCAGTTCATCCCGCTGGCAAAAAACAGCGTCATGCCGAGAAAGCCACCGGGAATAAAGGACAGTAACCAGAAACGCCCTT‑GCCAGACCACTACCGCCGAGAAGGGTACGGTAGTAATTACGCTCGCCCAGAACGCGGACAGTCCACTTACAGAGGCCAGCCAACCAGCAGTCAGCGCGGCGAAAAACGCCCACACCATCCCGGAATAATTAACCGCCAGACTTCTGATAAACCCCGATTTAC  >  NC_000913/334601‑334871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑