Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,121,744	A→G	S2P (TCT→CCT)	hslU ←	ATPase component of the HslVU protease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,121,744	0	A	G	100.0%	185.9 / NA	51	S2P (TCT→CCT)	hslU	ATPase component of the HslVU protease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/29);  total (22/29)

TGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGACATGGGAGATCCTTACGCTTTGTAGCTTAATTCTTCGATGGTGTGGAAATGGTTGGTATAGATGCAAATGTCGCCTGCAATATCCAACGCCTTTTCAGCAATTTCACGGGCGCTAAGTTCAGTG  >  NC_000913/4121611‑4121868                                                                                                                                      |                                                                                                                             tGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGGCATggg                                                                                                                        >  1:458537/1‑140 (MQ=255)  ggCGCAGCTCTTCGTTGAGCGGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGGCATGGga                                                                                                                       <  2:189165/140‑1 (MQ=255)      cAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGGCATGGGAGATc                                                                                                                   >  2:129811/1‑140 (MQ=255)         ctctTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGGCATGGGAGATCCtt                                                                                                                >  1:914031/1‑140 (MQ=255)                  gAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGGCATGGGAGATCCTTACGCTTTGt                                                                                                       >  2:570732/1‑140 (MQ=255)                     cTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGGCATGGGAGATCCTTACGCTTTGTAGc                                                                                                    >  2:359967/1‑140 (MQ=255)                       gCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGGCATGGGAGATCCTTACGCTTTGTAGCtt                                                                                                  <  1:570732/140‑1 (MQ=255)                        cATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGGCATGGGAGATCCTTACGCTTTGTAGCTTa                                                                                                 >  1:632022/1‑140 (MQ=255)                         aTGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGGCATGGGAGATCCTTACGCTTTGTAGCTTaa                                                                                                <  1:33529/140‑1 (MQ=255)                          tGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGGCATGGGAGATCCTTACGCTTTGTAGCTTAAt                                                                                               >  1:42829/1‑140 (MQ=255)                                gCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGGCATGGGAGATCCTTACGCTTTGTAGCTTAATTCTTCg                                                                                         <  2:914031/140‑1 (MQ=255)                                 ccAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGGCATGGGAGATCCTTACGCTTTGTAGCTTAATTCTTCGa                                                                                        <  1:129811/140‑1 (MQ=255)                                 ccAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGGCATGGGAGATCCTTACGCTTTGTAGCTTAATTCTTCGa                                                                                        <  2:42829/140‑1 (MQ=255)                                 ccAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGGCATGGGAGATCCTTACGCTTTGTAGCTTAATTCTTCGa                                                                                        <  2:550996/140‑1 (MQ=255)                                 ccAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGGCATGGGAGATCCTTACGCTTTGTAGCTTAATTCTTCGa                                                                                        <  1:873886/140‑1 (MQ=255)                                 ccAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGGCATGGGAGATCCTTACGCTTTGTAGCTTAATTCTTCGa                                                                                        <  1:469781/140‑1 (MQ=255)                                 ccAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGGCATGGGAGATCCTTACGCTTTGTAGCTTAATTCTTCGa                                                                                        <  2:930392/140‑1 (MQ=255)                                  cAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGGCATGGGAGATCCTTACGCTTTGTAGCTTAATTCTTCGAt                                                                                       >  2:585887/1‑140 (MQ=255)                                  cAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGGCATGGGAGATCCTTACGCTTTGTAGCTTAATTCTTCGAt                                                                                       <  1:887761/140‑1 (MQ=255)                                    gCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGGCATGGGAGATCCTTACGCTTTGTAGCTTAATTCTTCGATgg                                                                                     >  1:802363/1‑140 (MQ=255)                                           cGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGGCATGGGAGATCCTTACGCTTTGTAGCTTAATTCTTCGATGGTGTGGaa                                                                              <  2:632022/140‑1 (MQ=255)                                           cGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGGCATGGGAGATCCTTACGCTTTGTAGCTTAATTCTTCGATGGTGTGGaa                                                                              <  2:539035/140‑1 (MQ=255)                                           cGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGGCATGGGAGATCCTTACGCTTTGTAGCTTAATTCTTCGATGGTGTGGaa                                                                              <  2:490804/140‑1 (MQ=255)                                               gagCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGGCATGGGAGATCCTTACGCTTTGTAGCTTAATTCTTCGa                                                                                        <  1:936053/126‑1 (MQ=255)                                               gagCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGGCATGGGAGATCCTTACGCTTTGTAGCTTAATTCTTCGa                                                                                        >  2:936053/1‑126 (MQ=255)                                                agCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGGCATGGGAGATCCTTACGCTTTGTAGCTTAATTCTTCGATGGTGTGGAAAtggt                                                                         <  1:359967/140‑1 (MQ=255)                                                  cAATCGCCACAGAACGCTTGGCGTTGTCCTGGTCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGGCATGGGAGATCCTTACGCTTTGTAGCTTGATTCTTCGATGGTGTGGAAAtggttg                                                                       >  1:665673/1‑140 (MQ=255)                                                          acaGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGGCATGGGAGATCCTTACGCTTTGTAGCTTAATTCTTCGATGGTGTGGAAATGGTTGGTATAGAt                                                               >  1:541264/1‑140 (MQ=255)                                                                cGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGGCATGGGAGATCCTTACGCTTTGTAGCTTAATTCTTCGATGGTGTGGAAATGGTTGGTATAGATGCAAAt                                                         <  2:26010/140‑1 (MQ=255)                                                                                      tgatgTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGGCATGGGAGATCCTTACGCTTTGTAGCTTAATTCTTCGATGGTGTGGAAATGGTTGGTATAGATGCAAATGTCGCCTGCAATATCCAACGcc                                   >  2:96319/1‑140 (MQ=255)                                                                                      tgatgTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGGCATGGGAGATCCTTACGCTTTGTAGCTTAATTCTTCGATGGTGTGGAAATGGTTGGTATAGATGCAAATGTCGCCTGCAATATCCAACGcc                                   >  2:869976/1‑140 (MQ=255)                                                                                      tgatgTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGGCATGGGAGATCCTTACGCTTTGTAGCTTAATTCTTCGATGGTGTGGAAATGGTTGGTATAGATGCAAATGTCGCCTGCAATATCCAACGcc                                   >  2:478352/1‑140 (MQ=255)                                                                                      tgatgTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGGCATGGGAGATCCTTACGCTTTGTAGCTTAATTCTTCGATGGTGTGGAAATGGTTGGTATAGATGCAAATGTCGCCTGCAATATCCAACGcc                                   >  2:65258/1‑140 (MQ=255)                                                                                      tgatgTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGGCATGGGAGATCCTTACGCTTTGTAGCTTAATTCTTCGATGGTGTGGAAATGGTTGGTATAGATGCAAATGTCGCCTGCAATATCCAACGcc                                   >  1:44109/1‑140 (MQ=255)                                                                                          gtgCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGGCATGGGAGATCCTTACGCTTTGTAGCTTAATTCTTCGATGGTGTGGAAATGGTTGGTATAGATGCAAATGTCGCCTGCAATATCCAACGCCtttt                               <  2:458537/140‑1 (MQ=255)                                                                                           tgCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGGCATGGGAGATCCTTACGCTTTGTAGCTTAATTCTTCGATGGTGTGGAAATGGTTGGTATAGATGCAAATGTCGCCTGCAATATCCAACGCCTTTTc                              >  2:532582/1‑140 (MQ=255)                                                                                                aTCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGGCATGGGAGATCCTTACGCTTTGTAGCTTAATTCTTCGATGGTGTGGAAATGGTTGGTATAGATGCAAATGTCGCCTGCAATATCCAACGCCTTTTCAGCaa                         >  1:815591/1‑140 (MQ=255)                                                                                                aTCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGGCATGGGAGATCCTTACGCTTTGTAGCTTAATTCTTCGATGGTGTGGAAATGGTTGGTATAGATGCAAATGTCGCCTGCAATATCCAACGCCTTTTCAGCaa                         >  2:352689/1‑140 (MQ=255)                                                                                                aTCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGGCATGGGAGATCCTTACGCTTTGTAGCTTAATTCTTCGATGGTGT‑GAAATGGTTGGTATAGATGCAAATGTCGCCTGCAATATCCAACGCCTTTTCAGCAAt                        >  1:890728/1‑140 (MQ=255)                                                                                                             aCGATTTCGCGTGGGGTCATTTCAGGCATGGGAGATCCTTACGCTTTGTAGCTTAATTCTTCGATGGTGTGGAAATGGTTGGTATAGATGCAAATGTCGCCTGCAATATCCAACGCCTTTTCAGCAATTTCACGGGCGCt            <  2:802363/140‑1 (MQ=255)                                                                                                               gATTTCGCGTGGGGTCATTTCAGGCATGGGAGATCCTTACGCTTTGTAGCTTAATTCTTCGATGGTGTGGAAATGGTTGGTATAGATGCAAATGTCGCCTGCAATATCCAACGCCTTTTCAGCAATTTCACGGGCGCTaa          >  1:860903/1‑140 (MQ=255)                                                                                                                  ttCGCGTGGGGTCATTTCAGGCATGGGAGATCCTTACGCTTTGTAGCTTAATTCTTCGATGGTGTGGAAATGGTTGGTATAGATGCAAATGTCGCCTGCAATATCCAACGCCTTTTCAGCAATTTCACGGGCGCTAAGtt       <  2:815591/140‑1 (MQ=255)                                                                                                                   tCGCGTGGGGTCATTTCAGGCATGGGAGATCCTTACGCTTTGTAGCTTAATTCTTCGATGGTGTGGAAATGGTTGGTATAGATGCAAATGTCGCCTGCAATATCCAACGCCTTTTCAGCAATTTCACGGGCGCTAAGTTc      <  1:352689/140‑1 (MQ=255)                                                                                                                     gcgTGGGGTCATTTCAGGCATGGGAGATCCTTACGCTTTGTAGCTTAATTCTTCGATGGTGTGGAAATGGTTGGTATAGATGCAAATGTCGCCTGCAATATCCAACGCCTTTTCAGCAATTTCACGGGCGCTAAGTTCAg    <  1:96319/140‑1 (MQ=255)                                                                                                                     gcgTGGGGTCATTTCAGGCATGGGAGATCCTTACGCTTTGTAGCTTAATTCTTCGATGGTGTGGAAATGGTTGGTATAGATGCAAATGTCGCCTGCAATATCCAACGCCTTTTCAGCAATTTCACGGGCGCTAAGTTCAg    <  1:585887/140‑1 (MQ=255)                                                                                                                       gTGGGGTCATTTCAGGCATGGGAGATCCTTACGCTTTGTAGCTTAATTCTTCGATGGTGTGGAAATGGTTGGTATAGATGCAAATGTCGCCTGCAATATCCAACGCCTTTTCAGCAATTTCACGGGCGCTAAGTTCAgtg  <  2:541264/140‑1 (MQ=255)                                                                                                                       gTGGGGTCATTTCAGGCATGGGAGATCCTTACGCTTTGTAGCTTAATTCTTCGATGGTGTGGAAATGGTTGGTATAGATGCAAATGTCGCCTGCAATATCCAACGCCTTTTCAGCAATTTCACGGGCGCTAAGTTCAgtg  <  2:860903/140‑1 (MQ=255)                                                                                                                       gTGGGGTCATTTCAGGCATGGGAGATCCTTACGCTTTGTAGCTTAATTCTTCGATGGTGTGGAAATGGTTGGTATAGATGCAAATGTCGCCTGCAATATCCAACGCCTTTTCAGCAATTTCACGGGCGCTAAGTTCAgtg  <  2:44109/140‑1 (MQ=255)                                                                                                                       gTGGGGTCATTTCAGGCATGGGAGATCCTTACGCTTTGTAGCTTAATTCTTCGATGGTGTGGAAATGGTTGGTATAGATGCAAATGTCGCCTGCAATATCCAACGCCTTTTCAGCAATTTCACGGGCGCTAAGTTCAgtg  <  1:478352/140‑1 (MQ=255)                                                                                                                       gTGGGGTCATTTCAGGCATGGGAGATCCTTACGCTTTGTAGCTTAATTCTTCGATGGTGTGGAAATGGTTGGTATAGATGCAAATGTCGCCTGCAATATCCAACGCCTTTTCAGCAATTTCACGGGCGCTAAGTTCAgtg  <  1:532582/140‑1 (MQ=255)                                                                                                                         ggggTCATTTCAGGCATGGGAGATCCTTACGCTTTGTAGCTTAATTCTTCGATGGTGTGGAAATGGTTGGTATAGATGCAAATGTCGCCTGCAATATCCAACGCCTTTTCAGCAATTTCACGGGCGCTAAGTTCAg    >  2:606572/1‑136 (MQ=255)                                                                                                                         ggggTCATTTCAGGCATGGGAGATCCTTACGCTTTGTAGCTTAATTCTTCGATGGTGTGGAAATGGTTGGTATAGATGCAAATGTCGCCTGCAATATCCAACGCCTTTTCAGCAATTTCACGGGCGCTAAGTTCAg    <  1:606572/136‑1 (MQ=255)                                                                                                                                      |                                                                                                                             TGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGACATGGGAGATCCTTACGCTTTGTAGCTTAATTCTTCGATGGTGTGGAAATGGTTGGTATAGATGCAAATGTCGCCTGCAATATCCAACGCCTTTTCAGCAATTTCACGGGCGCTAAGTTCAGTG  >  NC_000913/4121611‑4121868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑