Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,336,134	T→C	T300A (ACG→GCG)	adiC ←	arginine:agmatine antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,336,134	0	T	C	100.0%	76.0 / NA	22	T300A (ACG→GCG)	adiC	arginine:agmatine antiporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/1);  total (21/1)

GCTGAGCTGGAAGATGGTCATCAAAATACCGACGATAATCAGCCCCGCCACTGGCGTACCCGCTTTATTTACACGGGCAAAAATCGGTGGGAACAGTCCGTCATCGGCAGCGGCTTTCGCCGTTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGAAGGAAACAATGGCCCCGGCGGTGTCACCCAACGCCATCCGTGCGGCATCACCGAATGGCGAAGCAGAAACGCG  >  NC_000913/4336012‑4336266                                                                                                                           |                                                                                                                                     gctgAGCTGGAAGATGGTCATCAAAATACCGACGATAATCAGCCCCGCCACTGGCGTACCCGCTTTATTTACACGGGCAAAAATCGGTGGGAACAGTCCGTCATCGGCAGCGGCTTTCGCCGCTTGACCCGCCAGCAACg                                                                                                                     >  1:15260/1‑140 (MQ=255)          gAAGATGGTCATCAAAATACCGACGATAATCAGCCCCGCCACTGGCGTACCCGCTTTATTTACACGGGCAAAAATCGGTGGGAACAGTCCGTCATCGGCAGCGGCTTTCGCCGCTTGACCCGCCAGCAACGTCCAgccgc                                                                                                            >  2:569320/1‑140 (MQ=255)          gAAGATGGTCATCAAAATACCGACGATAATCAGCCCCGCCACTGGCGTACCCGCTTTATTTACACGGGCAAAAATCGGTGGGAACAGTCCGTCATCGGCAGCGGCTTTCGCCGCTTGACCCGCCAGCAACGTCCAgccgc                                                                                                            >  1:459218/1‑140 (MQ=255)                              cgacgaTAATCAGCCCCGCCACTGGCGTACCCGCTTTATTTACACGGGCAAAAATCGGTGGGAACAGTCCGTCATCGGCAGCGGCTTTCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAAccc                                                                                        >  1:404100/1‑140 (MQ=255)                               gacgaTAATCAGCCCCGCCACTGGCGTACCCGCTTTATTTACACGGGCAAAAATCGGTGGGAACAGTCCGTCATCGGCAGCGGCTTTCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCg                                                                                       >  1:745329/1‑140 (MQ=255)                                acgaTAATCAGCCCCGCCACTGGCGTACCCGCTTTATTTACACGGGCAAAAATCGGTGGGAACAGTCCGTCATCGGCAGCGGCTTTCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGc                                                                                      >  2:384772/1‑140 (MQ=255)                                           ccccGCCACTGGCGTACCCGCTTTATTTACACGGGCAAAAATCGGTGGGAACAGTCCGTCATCGGCAGCGGCTTTCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGa                                                                           >  2:39891/1‑140 (MQ=255)                                                ccACTGGCGTACCCGCTTTATTTACACGGGCAAAAATCGGTGGGAACAGTCCGTCATCGGCAGCGGCTTTCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGAAGGaa                                                                      >  2:68925/1‑140 (MQ=255)                                                ccACTGGCGTACCCGCTTTATTTACACGGGCAAAAATCGGTGGGAACAGTCCGTCATCGGCAGCGGCTTTCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGAAGGaa                                                                      >  1:744453/1‑140 (MQ=255)                                                        gtccccGTTTTATTTCCACGGGCAAAAATCGGTGGGAACAGCCCGTCATCGGCAGCGGTTTTCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGAAGGAAACAATGGc                                                              <  2:744453/137‑1 (MQ=255)                                                           cccGCTTTTTTTACACGGGCAAAAATCGGTGGGAACAGTCCGTCATCGGCAGCGGCTTTCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGAAGGAAACAATGGcccc                                                           >  1:651482/1‑140 (MQ=255)                                                             cGCTTTATTTACAGGGGCAAAAATCGGTGGGAACAGTCCGTCATCGGCAGCGGCTTTCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGAAGGAAACAATGGCCCcgg                                                         <  2:606361/140‑1 (MQ=255)                                                                tttatttaCACGGGCAAAAATCGGTGGGAACAGTCCGTCATCGGCAGCGGCTTTCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGAAGGAAACAATGGCCCcggcgg                                                      >  2:305862/1‑140 (MQ=255)                                                                tttatttaCACGGGCAAAAATCGGTGGGAACAGTCCGTCATCGGCAGCGGCTTTCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGAAGGAAACAATGGCCCcggcgg                                                      >  1:457545/1‑140 (MQ=255)                                                                   atttaCACGGGAAAAAATCGGTGGGAACAGTCCGTCATCGGCAGCGGCTTTCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGAAGGAAACAATGGCCCCGGCGgtgt                                                   <  2:195313/140‑1 (MQ=255)                                                                       acacGGGCAAAAATCGGTGGGAACAGTCCGTCATCGGCAGCGGCTTTCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGAAGGAAACAATGGCCCCGGCGGTGTCAcc                                               <  1:305862/140‑1 (MQ=255)                                                                          cGGGCAAAAATCGGTGGGAACAGTCCGTCATCGGCAGCGGCTTTCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGAAGGAAACAATGGCCCCGGCGGTGTCACCCaa                                            >  2:643341/1‑140 (MQ=255)                                                                            ggCAAAAATCGGTGGGAACAGTCCGTCATCGGCAGCGGCTTTCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGAAGGAAACAATGGCCCCGGCGGTGTCACCCAACg                                          >  2:159246/1‑140 (MQ=255)                                                                             gCAAAAATCGGTGGGAACAGTCCGTCATCGGCAGCGGCTTTCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGAAGGAAACAATGGCCCCGGCGGTGTCACCCAACGc                                         >  2:236945/1‑140 (MQ=255)                                                                                       gTGGGAACAGTCCGTCATCGGCAGCGGCTTTCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGAAGGAAACAATGGCCCCGGCGGTGTCACCCAACGCCATCCGTGCg                               >  1:191178/1‑140 (MQ=255)                                                                                        ggggAACAGCCCGCCACGGGCAGCGGCTTCCGCCGCTTGCCCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGAAGGAAACAATGGCCCCGGCGGTGTCACCCAACGCCATCCGTGCgg                              <  2:457545/139‑1 (MQ=255)                                                                                                gTCCGTCATCGGCAGCGGCTTTCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGAAGGAAACAATGGCCCCGGCGGTGTCACCCAACGCCATCCGTGCGGCATCACCg                      >  2:75682/1‑140 (MQ=255)                                                                                                  ccGTCATCGGCAGCGGCTTTCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGAAGGAAACAATGGCCCCGGCGGTGTCACCCAACGCCATCCGTGCGGCATCACCGaa                    <  2:513982/140‑1 (MQ=255)                                                                                                     ccagcggcggcggCTTTCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGAAGGAAACAATGGCCCCGGCGGTGTCACCCAACGCCATCCGTGCGGCATCACCGAATgg                 <  2:745329/136‑1 (MQ=255)                                                                                                         cGGCAGCGGCTTTCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGAAGGAAACAATGGCCCCGGCGGTGTCACCCAACGCCATCCGTGCGGCATCACCGAATGGCGaa             >  2:9235/1‑140 (MQ=255)                                                                                                           gCAGCGGCTTTCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGAAGGAAACAATGGCCCCGGCGGTGTCACCCAACGCCATCCGTGCGGCATCACCGAATGGCGAAGc           >  2:213809/1‑140 (MQ=255)                                                                                                           gCAGCGGCTTTCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGAAGGAAACAATGGCCCCGGCGGTGTCACCCAACGCCATCCGTGCGGCATCACCGAATGGCGAAGc           >  1:150416/1‑140 (MQ=255)                                                                                                               cGGCTTTCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGAAGGAAACAATGGCCCCGGCGGTGTCACCCAACGCCATCCGTGCGGCATCACCGAATGGCGAAGCAGaa       >  2:236192/1‑140 (MQ=255)                                                                                                                    ttCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGAAGGAAACAATGGCCCCGGCGGTGTCACCCAACGCCATCCGTGCGGCATCACCGAATGGCGAAGCAGAAAcgcg  <  2:150416/140‑1 (MQ=255)                                                                                                                    ttCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGAAGGAAACAATGGCCCCGGCGGTGTCACCCAACGCCATCCGTGCGGCATCACCGAATGGCGAAGCAGAAAcgcg  <  1:776571/140‑1 (MQ=255)                                                                                                                    ttCGCCGCTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGAAGGAAACAATGGCCCCGGCGGTGTCACCCAACGCCATCCGTGCGGCATCACCGAATGGCGAAGCAGAAAcgcg  <  1:75682/140‑1 (MQ=255)                                                                                                                           |                                                                                                                                     GCTGAGCTGGAAGATGGTCATCAAAATACCGACGATAATCAGCCCCGCCACTGGCGTACCCGCTTTATTTACACGGGCAAAAATCGGTGGGAACAGTCCGTCATCGGCAGCGGCTTTCGCCGTTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAACCCGCAGCTGCGCAGAAGGAAACAATGGCCCCGGCGGTGTCACCCAACGCCATCCGTGCGGCATCACCGAATGGCGAAGCAGAAACGCG  >  NC_000913/4336012‑4336266

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑