Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,430,909	C→T	T349M (ACG→ATG)	cycA →	serine/alanine/glycine/cycloserine:H(+)symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,430,909	0	C	T	100.0%	243.1 / NA	68	T349M (ACG→ATG)	cycA	serine/alanine/glycine/cycloserine:H(+)symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (37/31);  total (37/31)

CTGACCTCTGCGGCGTCTTCCGCTAACAGCGGCGTCTTCTCTACCAGCCGTATGCTGTTTGGTCTGGCGCAGGAAGGTGTGGCACCGAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGACGTTCTCGTGTATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAATCCTAGTGTGATTGGCGCGTTCACGATGATTACAACCGTTTCCGCGATTCTGTTTATGTTCGTCTGGACGATTATCCTTTGCTCGT  >  NC_000913/4430773‑4431043                                                                                                                                         |                                                                                                                                       cTGACCTCTGCGGCGTCTTCCGCTAACAGCGGCGTCTTCTCTACCAGCCGTATGCTGTTTGGTCTGGCGCAGGGAGGTGTGGCACCGAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGATGtt                                                                                                                                     >  1:218836/1‑140 (MQ=255) cTGACCTCTGCGGCGTCTTCCGCTAACAGCGGCGTCTTCTCTACCAGCCGTATGCTGTTTGGTCTGGCGCAGGAAGGTGTGGCACCGAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGATGtt                                                                                                                                     >  2:491877/1‑140 (MQ=255) cTGACCTCTGCGGCGTCTTCCGCTAACAGCGGCGTCTTCTCTACCAGCCGTATGCTGTTTGGTCTGGCGCAGGAAGGTGTGGCACCGAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGATGtt                                                                                                                                     >  2:389419/1‑140 (MQ=255)    aCCTCTGCGGCGTCTTCCGCTAACAGCGGCGTCTTCTCTACCAGCCGTATGCTGTTTGGTCTGGCGCAGGAAGGTGTGGCACCGAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGATGTtctc                                                                                                                                  >  2:495120/1‑140 (MQ=255)         tGCGGCGTCTTCCGCTAACAGCGGCGTCTTCTCTACCAGCCGTATGCTGTTTGGTCTGGCGCAGGAAGGTGTGGCACCGAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGATGTTCTCGTGTa                                                                                                                             >  1:309831/1‑140 (MQ=255)         tGCGGCGTCTTCCGCTAACAGCGGCGTCTTCTCTACCAGCCGTATGCTGTTTGGTCTGGCGCAGGAAGGTGTGGCACCGAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGATGTTCTCGTGTa                                                                                                                             >  1:762704/1‑140 (MQ=255)           cggcgTCTTCCGCTAACAGCGGCGTCTTCTCTACCAGCCGTATGCTGTTTGGTCTGGCGCAGGAAGGTGTGGCACCGAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGATGTTCTCGTGTAtc                                                                                                                           >  2:9797/1‑140 (MQ=255)              cgTCTTCCGCTAACAGCGGCGTCTTCTCTACCAGCCGTATGCTGTTTGGTCTGGCGCAGGAAGGTGTGGCACCGAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGATGTTCTCGTGTAtctgt                                                                                                                        >  2:443135/1‑140 (MQ=255)              cgTCTTCCGCTAACAGCGGCGTCTTCTCTACCAGCCGTATGCTGTTTGGTCTGGCGCAGGAAGGTGTGGCACCGAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGATGTTCTCGTGTAtctgt                                                                                                                        >  2:463446/1‑140 (MQ=255)                     cGCTAACAGCGGCGTCTTCTCTACCAGCCGTATGCTGTTTGGTCTGGCGCAGGAAGGTGTGGCACCGAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGATGTTCTCGTGTATCTGTCTGCTcg                                                                                                                 <  2:762704/140‑1 (MQ=255)                      gCTAACAGCGGCGTCTTCTCTACCAGCCGTATGCTGTTTGGTCTGGCGCAGGAAGGTGTGGCACCGAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGATGTTCTCGTGTATCTGTCTGCTcgg                                                                                                                <  2:852351/140‑1 (MQ=255)                           cAGCGGCGTCTTCTCTACCAGCCGTATGCTGTTTGGTCTGGCGCAGGAAGGTGTGGCACCGAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGATGTTCTCGTGTATCTGTCTGCTCGgcggcg                                                                                                           <  2:309831/140‑1 (MQ=255)                           cAGCGGCGTCTTCTCTACCAGCCGTATGCTGTTTGGTCTGGCGCAGGAAGGTGTGGCACCGAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGATGTTCTCGTGTATCTGTCTGCTCGgcggcg                                                                                                           <  2:699766/140‑1 (MQ=255)                           cAGCGGCGTCTTCTCTACCAGCCGTATGCTGTTTGGTCTGGCGCAGGAAGGTGTGGCACCGAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGATGTTCTCGTGTATCTGTCTGCTCGgcggcg                                                                                                           <  2:238517/140‑1 (MQ=255)                              cggcgTCTTCTCTACCAGCCGTATGCTGTTTGGTCTGGCGCAGGAAGGTGTGGCACCGAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGATGTTCTCGTGTATCTGTCTGCTCGGCGGCgtgg                                                                                                        >  1:741942/1‑140 (MQ=255)                              cggcgTCTTCTCTACCAGCCGTATGCTGTTTGGTCTGGCGCAGGAAGGTGTGGCACCGAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGATGTTCTCGTGTATCTGTCTGCTCGGCGGCgtgg                                                                                                        >  1:900406/1‑140 (MQ=255)                               ggcgTCTTCTCTACCAGCCGTATGCTGTTTGGTCTGGCGCAGGAAGGTGTGGCACCGAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGATGTTCTCGTGTATCTGTCTGCTCGGCGGCgtggt                                                                                                       <  1:443135/140‑1 (MQ=255)                               ggcgTCTTCTCTACCAGCCGTATGCTGTTTGGTCTGGCGCAGGAAGGTGTGGCACCGAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGATGTTCTCGTGTATCTGTCTGCTCGGCGGCgtggt                                                                                                       <  2:900406/140‑1 (MQ=255)                                 cgTCTTCTCTACCAGCCGTATGCTGTTTGGTCTGGCGCAGGAAGGTGTGGCACCGAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGATGTTCTCGTGTATCTGTCTGCTCGGCGGCGTGGTGa                                                                                                     <  2:636830/140‑1 (MQ=255)                                 cgTCTTCTCTACCAGCCGTATGCTGTTTGGTCTGGCGCAGGAAGGTGTGGCACCGAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGATGTTCTCGTGTATCTGTCTGCTCGGCGGCGTGGTGa                                                                                                     <  1:896765/140‑1 (MQ=255)                                           aCCAGCCGTATGCTGTTTGGTCTGGCGCAGGAAGGTGTGGCACCGAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGATGTTCTCGTGTATCTGTCTGCTCGGCGGCGTGGTGATGTTGTAtgt                                                                                           <  1:42625/140‑1 (MQ=255)                                           aCCAGCCGTATGCTGTTTGGTCTGGCGCAGGAAGGTGTGGCACCGAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGATGTTCTCGTGTATCTGTCTGCTCGGCGGCGTGGTGATGTTGTAtgt                                                                                           <  1:235069/140‑1 (MQ=255)                                           aCCAGCCGTATGCTGTTTGGTCTGGCGCAGGAAGGTGTGGCACCGAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGATGTTCTCGTGTATCTGTCTGCTCGGCGGCGTGGTGATGTTGTAtgt                                                                                           <  2:9502/140‑1 (MQ=255)                                              aGCCGTATGCTGTTTGGTCTGGCGCAGGAAGGTGTGGCACCGAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGATGTTCTCGTGTATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGaa                                                                                        <  1:865046/140‑1 (MQ=255)                                              aGCCGTATGCTGTTTGGTCTGGCGCAGGAAGGTGTGGCACCGAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGATGTTCTCGTGTATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGaa                                                                                        <  1:9797/140‑1 (MQ=255)                                               gCCGTATGCTGTTTGGTCTGGCGCAGGAAGGTGTGGCACCGAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGATGTTCTCGTGTATCTGTCTGCTCGGCGGCgtggt                                                                                                       >  1:495131/1‑124 (MQ=255)                                               gCCGTATGCTGTTTGGTCTGGCGCAGGAAGGTGTGGCACCGAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGATGTTCTCGTGTATCTGTCTGCTCGGCGGCgtggt                                                                                                       <  2:495131/124‑1 (MQ=255)                                               gCCGTATGCTGTTTGGTCTGGCGCAGGAAGGTGTGGCACCGAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGATGTTCTCGTGTATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAAt                                                                                       >  1:251752/1‑140 (MQ=255)                                               gCCGTATGCTGTTTGGTCTGGCGCAGGAAGGTGTGGCACCGAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGATGTTCTCGTGTATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAAt                                                                                       >  1:933856/1‑140 (MQ=255)                                                    aTGCTGTTTGGTCTGGCGCAGGAAGGTGTGGCACCGAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGATGTTCTCGTGTATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAATCCTAg                                                                                  >  2:424539/1‑140 (MQ=255)                                                    aTGCTGTTTGGTCTGGCGCAGGAAGGTGTGGCACCGAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGATGTTCTCGTGTATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAATCCTAg                                                                                  >  1:938800/1‑140 (MQ=255)                                                          tttGGTCTGGCGCAGGAAGGTGTGGCACCGAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGATGTTCTCGTGTATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAATCCTAGTGTGAt                                                                            >  2:183226/1‑140 (MQ=255)                                                              gTCTGGCGCAGGAAGGTGTGGCACCGAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGATGTTCTCGTGTATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAATCCTAGTGTGATTGgc                                                                        >  2:123426/1‑140 (MQ=255)                                                               tCTGGCGCAGGAAGGTGCGGCACCGAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGATGTTCTCGTGTATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAATCCTAGTGTGATTGgcg                                                                       >  1:946171/1‑140 (MQ=255)                                                                 tGGCGCAGGAAGGTGTGGCACCGAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGATGTTCTCGTGTATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAAt                                                                                       >  1:276323/1‑122 (MQ=255)                                                                 tGGCGCAGGAAGGTGTGGCACCGAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGATGTTCTCGTGTATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAAt                                                                                       <  2:276323/122‑1 (MQ=255)                                                                    cgcAGGAAGGTGTGGCACCGAAAGCGTTCGCTAAACTTTTTAAGCGCGCAGTACCCGCGAAAGGGCTGATGTTCTCGTGTATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAATCCTAGTGTGATTGGCGCGTTc                                                                  >  2:208686/1‑140 (MQ=255)                                                                              tgtgGCACCGAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGATGTTCTCGTGTATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAATGCTAGTGTGATTGGCGAGTTCACGATGAGTa                                                        >  2:583156/1‑140 (MQ=255)                                                                              tgtgGCACCGAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGATGTTCTCGTGTATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAATCCTAGTGTGATTGGCGCGTTCACGATGATTa                                                        >  1:335800/1‑140 (MQ=255)                                                                                       gAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGATGTTCTCGTGTATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAATCCTAGTGTGATTGGCGCGTTCACGATGATTACAACCGttt                                               >  1:101500/1‑140 (MQ=255)                                                                                        aaaGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGATGTTCTCGTGTATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAATCCTAGTGTGATTGGCGCGTTCACGATGATTACAACCGTTTc                                              >  2:628380/1‑140 (MQ=255)                                                                                          aGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGATGTTCTCGTGTATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAATCCTAGTGTGATTGGCGCGTTCACGATGATTACAACCGTTTCcg                                            <  2:678377/140‑1 (MQ=255)                                                                                             gTTCGCTAAACTTTCTAAGCGCGCAGTGCCCGCGAAAGGGCTGATGTTCTCGTGTATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAATCCTAGTGTGATTGGCGCGTTCACGATGATTACAACCGTTTCCGCGa                                         <  2:850904/140‑1 (MQ=255)                                                                                              ttCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGATGTTCTCGTGTATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAATCCTAGTGTGATTGGCGCGTTCACGATGATTACAACCGTTTCCGCGAt                                        <  1:424539/140‑1 (MQ=255)                                                                                               tCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGATGTTCTCGTGTATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAATCCTAGTGTGATTGGCGCGTTCACGATGATTACAACCGTTTCCGCGAtt                                       <  2:251752/140‑1 (MQ=255)                                                                                               tCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGATGTTCTCGTGTATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAATCCTAGTGTGATTGGCGCGTTCACGATGATTACAACCGTTTCCGCGAtt                                       >  1:625242/1‑140 (MQ=255)                                                                                               tCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGATGTTCTCGTGTATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAATCCTAGTGTGATTGGCGCGTTCACGATGATTACAACCGTTTCCGCGAtt                                       >  1:34829/1‑140 (MQ=255)                                                                                                cGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGATGTTCTCGTGTATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAATCCTAGTGTGATTGGCGCGTTCACGATGATTACAACCGTTTCCGCGATTc                                      <  2:311706/140‑1 (MQ=255)                                                                                                         ttCTAAGCGCGCAGTACCCGCGAAAGGGCTGATGTTCTCGTGTATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAATCCTAGTGTGATTGGCGCGTTCACGATGATTACAACCGTTTCCGCGATTCTGTTTATGt                             <  2:335800/140‑1 (MQ=255)                                                                                                          tCTAAGCGCGCAGTACCCGCGAAAGGGCTGATGTTCTCGTGTATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAATCCTAGTGTGATTGGCGCGTTCACGATGATTACAACCGTTTCCGCGATTCTGTTTATGtt                            >  1:424627/1‑140 (MQ=255)                                                                                                                cgcgcAGTACCCGCGAAAGGGCTGATGTTCTCGTGTATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAATCCTAGTGTGATTGGCGCGTTCACGATGATTACAACCGTTTCCGCGATTCTGTTTATGTTCGTCTg                      <  2:741942/140‑1 (MQ=255)                                                                                                                cgcgcAGTACCCGCGAAAGGGCTGATGTTCTCGTGTATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAATCCTAGTGTGATTGGCGCGTTCACGATGATTACAACCGTTTCCGCGATTCTGTTTATGTTCGTCTg                      <  1:498398/140‑1 (MQ=255)                                                                                                                 gcgcAGTACCCGCGAAAGGGCTGATGTTCTCGTGTATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAATCCTAGTGTGATTGGCGCGTTCACGATGATTACAACCGTTTCCGCGATTCTGTTTATGTTCGTCTgg                     <  1:583156/140‑1 (MQ=255)                                                                                                                  cgcAGTACCCGCGAAAGGGCTGATGTTCTCGTGTATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAATCCTAGTGTGATTGGCGCGTTCACGATGATTACAACCGTTTCCGCGATTCTGTTTATGTTCGTCTGGa                    <  1:389419/140‑1 (MQ=255)                                                                                                                  cgcAGTACCCGCGAAAGGGCTGATGTTCTCGTGTATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAATCCTAGTGTGATTGGCGCGTTCACGATGATTACAACCGTTTCCGCGATTCTGTTTATGTTCGTCTGGa                    <  2:34829/140‑1 (MQ=255)                                                                                                                  cgcAGTACCCGCGAAAGGGCTGATGTTCTCGTGTATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAATCCTAGTGTGATTGGCGCGTTCACGATGATTACAACCGTTTCCGCGATTCTGTTTATGTTCGTCTGGa                    >  1:311042/1‑140 (MQ=255)                                                                                                                  cgcAGTACCCGCGAAAGGGCTGATGTTCTCGTGTATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAATCCTAGTGTGATTGGCGCGTTCACGATGATTACAACCGTTTCCGCGATTCTGTTTATGTTCGTCTGGa                    <  2:311042/140‑1 (MQ=255)                                                                                                                          ccGCGAAAGGGCTGATGTTCTCGTGTATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAATCCTAGTGTGATTGGCGCGTTCACGATGATTACAACCGTTTCCGCGATTCTGTTTATGTTCGTCTGGACGATTATc            >  1:591627/1‑140 (MQ=255)                                                                                                                            gcgAAAGGGCTGATGTTCTCGTGTATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAATCCTAGTGTGATTGGCGCGTTCACGATGATTACAACCGTTTCCGCGATTCTGTTTATGTTCGTCTGGACGATTATCCt          <  2:591627/140‑1 (MQ=255)                                                                                                                            gcgAAAGGGCTGATGTTCTCGTGTATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAATCCTAGTGTGATTGGCGCGTTCACGATGATTACAACCGTTTCCGCGATTCTGTTTATGTTCGTCTGGACGATTATCCt          <  2:938800/140‑1 (MQ=255)                                                                                                                             cgAAAGGGCTGATGTTCTCGTGTATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAATCCTAGTGTGATTGGCGCGTTCACGATGATTACAACCGTTTCCGCGATTCTGTTTATGTTCGTCTGGACGATTATCCtt         >  2:924591/1‑140 (MQ=255)                                                                                                                               aaaGGGCTGATGTTCTCGTGTATCTGTCTGCTCGGCGGCGTGGAGATGTTGTATGTGAATCCTAGTGTGATTGGCGCGTTCACGATGATTACAACCGTTTCCGCGATTCTGTTTATGTTCGTCTGGACGATTATCCTTTg       >  2:685590/1‑140 (MQ=255)                                                                                                                                 aGGGCTGATGTTCTCGTGTATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAATCCTAGTGTGATTGGCGCGTTCACGATGATTACAACCGTTTCCGCGATTCTGTTTATGTTCGTCTGGACGATTATCCTTTGCt     >  2:174750/1‑140 (MQ=255)                                                                                                                                 aGGGCTGATGTTCTCGTGTATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAATCCTAGTGTGATTGGCGCGTTCACGATGATTACAACCGTTTCCGCGATTCTGTTTATGTTCGTCTGGACGATTATCCTTTGCt     >  1:128543/1‑140 (MQ=255)                                                                                                                                 aGGGCTGATGTTCTCGTGTATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAATCCTAGTGTGATTGGCGCGTTCACGATGATTACAACCGTTTCCGCGATTCTGTTTATGTTCGTCTGGACGATTATCCTTTGCt     >  1:855815/1‑140 (MQ=255)                                                                                                                                 aGGGCTGATGCTCTCGTGTATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAATCCTAGTGTGATTGGCGCGTTCACGATGATTACAACCGTTTCCGCGATTCTGTTTATGTTCGCCTGGACGATTATCCTTTGCt     >  2:329479/1‑140 (MQ=255)                                                                                                                                   ggCTGATGTTCTCGTGTATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAATCCTAGTGTGATTGGCGCGTTCACGATGATTACAACCGTTTCCGCGATTCTGTTTATGTTCGTCTGGACGATTATCCTTTGCTCg   >  1:117585/1‑140 (MQ=255)                                                                                                                                    gCTGATGTTCTCGTGTATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAATCCTAGTGTGATTGGCGCGTTCACGATGATTACAACCGTTTCCGCGATTCTGTTTATGTTCGTCTGGACGATTATCCTTTGCTCGt  <  1:183226/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       CTGACCTCTGCGGCGTCTTCCGCTAACAGCGGCGTCTTCTCTACCAGCCGTATGCTGTTTGGTCTGGCGCAGGAAGGTGTGGCACCGAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGGGCTGACGTTCTCGTGTATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAATCCTAGTGTGATTGGCGCGTTCACGATGATTACAACCGTTTCCGCGATTCTGTTTATGTTCGTCTGGACGATTATCCTTTGCTCGT  >  NC_000913/4430773‑4431043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑