| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 398,684 | A→G | E271E (GAA→GAG) | yaiW → | surface‑exposed outer membrane lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 398,684 | 0 | A | G | 98.0% | 174.3 / ‑4.3 | 51 | E271E (GAA→GAG) | yaiW | surface‑exposed outer membrane lipoprotein |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (29/21); total (30/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01 | |||||||||||
CCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTTAGAGAAAGGTGACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCA > NC_000913/398550‑398818 | ccGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAGCCCgg > 1:178717/1‑140 (MQ=255)ccGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAGCCCgg > 2:883706/1‑140 (MQ=255)ccGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAGCCCgg > 1:559113/1‑140 (MQ=255)ccGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCgg > 1:179743/1‑140 (MQ=255) taCCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAGCCCGGGAAAAc < 1:630698/140‑1 (MQ=255) ttttGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAGCCCGGGAAAACCGAAc > 1:216815/1‑140 (MQ=255) ttttGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAGCCCGGGAAAACCGAAc > 1:748835/1‑140 (MQ=255) ttttGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAGCCCGGGAAAACCGAAc > 2:386402/1‑140 (MQ=255) aTTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAGCCCGGGAAAACCGAACTGGCAAcg > 2:859746/1‑140 (MQ=255) aCGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAGCCCGGGAAAACCGAACTGGCAACGCGCaaa < 1:859746/140‑1 (MQ=255) gctggTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAGCCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCt > 1:709586/1‑140 (MQ=255) gctggTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAGCCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCt > 1:942447/1‑140 (MQ=255) ctggTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAGCCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTg > 1:3408/1‑140 (MQ=255) tggTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAGCCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGc < 1:883706/140‑1 (MQ=255) tggTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAGCCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGc < 2:178717/140‑1 (MQ=255) cAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAGCCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTgg > 1:783245/1‑140 (MQ=255) cAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAGCCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTgg > 2:899769/1‑140 (MQ=255) ccGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAGCCCGGGAAAACCGAACTGGCAACGCGCAAACt > 2:368949/1‑123 (MQ=255) ccGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAGCCCGGGAAAACCGAACTGGCAACGCGCAAACt < 1:368949/123‑1 (MQ=255) gCCGCGTTTCAGAACGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAGCCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGa < 1:386402/140‑1 (MQ=255) cgTTTCAGAACGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAGCCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGc > 2:172287/1‑140 (MQ=255) cgcgGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAGCCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGcc < 2:559113/140‑1 (MQ=255) cgGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAGCCCGGGAAAACCGAACTGGCAACGCGCAAACTGGTTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGt < 1:899769/140‑1 (MQ=255) cgGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAGCCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGt < 2:709586/140‑1 (MQ=255) cgGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTCATTTAATTCGTTACGACAGTAAAGAGCCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGt < 2:942447/140‑1 (MQ=255) tCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAGCCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAg > 1:775382/1‑140 (MQ=255) gcggcgTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAGCCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTTAGAGAAAggg > 2:644056/1‑139 (MQ=255) gcggcgTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAGCCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTTAGAGAAAGGt > 2:243759/1‑140 (MQ=255) gcggcgTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAGCCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTTAGAGAAAGGt > 1:51290/1‑140 (MQ=255) gcggcgTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAGCCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTTAGAGAAAGGt > 2:425763/1‑140 (MQ=255) ggcgTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAGCCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAg < 1:699473/126‑1 (MQ=255) ggcgTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAGCCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAg > 2:699473/1‑126 (MQ=255) ggcgTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAGCCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTTAGAGAAAGGTGa < 1:172287/140‑1 (MQ=255) ggcgTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAGCCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTTAGAGAAAGGTGa > 2:80260/1‑140 (MQ=255) gcgTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAGCCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTTAGAGAAAGGTGAc < 2:51290/140‑1 (MQ=255) tGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAGCCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTTAGAGAAAGGTGACAGc > 2:383469/1‑140 (MQ=255) ggCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAGCCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTTAGAGAAAGGTGACAGCTTCTCtt < 1:425763/140‑1 (MQ=255) gACGGTGATTTAATTCGTTACGACAGTAAAGAGCCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTTAGAGAAAGGTGACAGCTTCTCTTTTgagga > 1:749394/1‑140 (MQ=255) cGGTGATTTAATTCGTTACGACAGTAAAGAGCCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTTAGAGAAAGGTGACAGCTTCTCTTTTGAGgaga < 2:748835/140‑1 (MQ=255) aTTTAATTCGTTACGACAGTAAAGAGCCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTTAGAGAAAGGTGACAGCTTCTCTTTTGAGGAGACGgcg < 2:687270/140‑1 (MQ=255) aTTTAATTCGTTACGACAGTAAAGAGCCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTTAGAGAAAGGTGACAGCTTCTCTTTTGAGGAGACGgcg < 2:501967/140‑1 (MQ=255) aTTTAATTCGTTACGACAGTAAAGAGCCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTTAGAGAAAGGTGACAGCTTCTCTTTTGAGGAGACGgcg < 2:783245/140‑1 (MQ=255) tAATTCGTTACGACAGTAAAGAGCCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTTAGAGAAAGGTGACAGCTTCTCTTTTGAGGAGACGgcg < 2:674266/137‑1 (MQ=255) tAATTCGTTACGACAGTAAAGAGCCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTTAGAGAAAGGTGACAGCTTCTCTTTTGAGGAGACGgcg > 1:674266/1‑137 (MQ=255) ttACGACAGTAAAGAGCCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTTAGAGAAAGGTGACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGa > 1:401380/1‑140 (MQ=255) tACGACAGTAAAGAGCCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTTAGAGAAAGGTGACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGaa < 2:3408/140‑1 (MQ=255) aGTAAAGAGCCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTTAGAGAAAGGTGACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTa < 1:243759/140‑1 (MQ=255) tAAAGAGCCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTTAGAGAAAGGTGACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATc > 1:463036/1‑140 (MQ=255) tAAAGAGCCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTTAGAGAAAGGTGACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATc > 2:423484/1‑140 (MQ=255) tAAAGAGCCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTTAGAGAAAGGTGACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATc > 1:158047/1‑140 (MQ=255) aaaGAGCCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTTAGAGAAAGGTGACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCa > 2:805203/1‑140 (MQ=255) | CCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTTAGAGAAAGGTGACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCA > NC_000913/398550‑398818 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |