Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	494,093	T→C	G6G (GGT→GGC)	ybaB →	putative nucleoid‑associated protein YbaB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	494,093	0	T	C	100.0%	216.1 / NA	60	G6G (GGT→GGC)	ybaB	putative nucleoid‑associated protein YbaB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/24);  total (36/24)

ATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTGACCATCAACGGTGCACACA  >  NC_000913/493959‑494226                                                                                                                                       |                                                                                                                                      atatTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGCCTggg                                                                                                                                  <  2:158985/140‑1 (MQ=255)  tatTCAAACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGCCTGGGt                                                                                                                                 <  2:413021/140‑1 (MQ=255)       aGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGCCTGGGTAACCt                                                                                                                            >  1:559842/1‑140 (MQ=255)         aCCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGCCTGGGTAACCtga                                                                                                                          >  1:146694/1‑140 (MQ=255)         aCCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGCCTGGGTAACCtga                                                                                                                          >  1:880360/1‑140 (MQ=255)          cccTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGCCTGGGTAACCtgat                                                                                                                         <  2:850353/140‑1 (MQ=255)             tGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGCCTGGGTAACCTGATGaa                                                                                                                      >  2:481599/1‑140 (MQ=255)             tGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCAGCCTGGGTAACCTGATGaa                                                                                                                      >  2:468168/1‑140 (MQ=255)              gCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGCCTGGGTAACCtgat                                                                                                                         <  1:197461/136‑1 (MQ=255)              gCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGCCTGGGTAACCtgat                                                                                                                         >  2:197461/1‑136 (MQ=255)              gCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGCCTGGGTAACCTGATGaag                                                                                                                     >  1:257560/1‑140 (MQ=255)              gCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGCCTGGGTAACCTGATGaag                                                                                                                     >  1:732721/1‑140 (MQ=255)               cGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGCCTGGGTAACCTGATGaagc                                                                                                                    >  2:874286/1‑140 (MQ=255)               cGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATGGAGAGAGAAACCTATGTTTGGTAAAGGCGGCCTGGGTAACCTGATGaagc                                                                                                                    >  1:765173/1‑140 (MQ=255)                          cGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGCCTGGGTAACCTGATGaagcaag                                                                                                                 >  1:584998/1‑132 (MQ=255)                          cGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGCCTGGGTAACCTGATGaagcaag                                                                                                                 <  2:584998/132‑1 (MQ=255)                               cGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGCCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCa                                                                                                    <  2:715146/140‑1 (MQ=255)                                            agaaAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGCCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAaaaatgcag                                                                                       >  2:355357/1‑140 (MQ=255)                                            agaaAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGCCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAaaaatgcag                                                                                       >  2:118233/1‑140 (MQ=255)                                            agaaAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGCCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAaaaatgcag                                                                                       >  1:263724/1‑140 (MQ=255)                                                 gTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGCCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAaaaatgcaga                                                                                      <  2:64040/136‑1 (MQ=255)                                                 gTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGCCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAaaaatgcaga                                                                                      >  1:64040/1‑136 (MQ=255)                                                  tATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGCCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAaaaatgcagaaaatg                                                                                 <  1:599707/140‑1 (MQ=255)                                                  tATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGCCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAaaaatgcagaaaatg                                                                                 <  2:146694/140‑1 (MQ=255)                                                          ccATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGCCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAaga                                                                         >  1:494035/1‑140 (MQ=255)                                                                     tAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGCCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGc                                                              >  2:12149/1‑140 (MQ=255)                                                                            aGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGCCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGt                                                       >  1:295619/1‑140 (MQ=255)                                                                              cTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGCCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCa                                                     >  1:610736/1‑140 (MQ=255)                                                                              cTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGCCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCa                                                     <  1:481599/140‑1 (MQ=255)                                                                              cTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGCCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCa                                                     <  2:880360/140‑1 (MQ=255)                                                                                   gTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGCCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGc                                                <  2:732721/140‑1 (MQ=255)                                                                                   gTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGCCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGc                                                >  2:115286/1‑140 (MQ=255)                                                                                      cGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGCCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGaa                                             >  1:307539/1‑140 (MQ=255)                                                                                        tCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGCCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATc                                           >  2:640716/1‑140 (MQ=255)                                                                                        tCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGCCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATc                                           <  1:115286/140‑1 (MQ=255)                                                                                        tCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGCCTGAGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATc                                           >  1:753270/1‑140 (MQ=255)                                                                                              ttAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGCCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGgcgc                                     <  2:295619/140‑1 (MQ=255)                                                                                                aaCGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGCCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAg                                   >  2:148475/1‑140 (MQ=255)                                                                                                 aCGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGCCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAgg                                  <  2:765173/140‑1 (MQ=255)                                                                                                 aCGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGCCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAgg                                  <  2:257560/140‑1 (MQ=255)                                                                                                      aTTGAGAGAGAAACCTATGTTTGGTAAAGGCGGCCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTgg                             >  2:146175/1‑140 (MQ=255)                                                                                                      aTTGAGAGAGAAACCTATGTTTGGTAAAGGCGGCCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTgg                             >  1:844884/1‑140 (MQ=255)                                                                                                      aTTGAGAGAGAAACCTATGTTTGGTAAAGGCGGCCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTgg                             >  1:766902/1‑140 (MQ=255)                                                                                                      aTTGAGAGAGAAACCTATGTTTGGTAAAGGCGGCCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTgg                             >  2:331614/1‑140 (MQ=255)                                                                                                      aTTGAGAGAGAAACCTATGTTTGGTAAAGGCGGCCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTgg                             >  2:475233/1‑140 (MQ=255)                                                                                                          agagagaAACCTATGTTTGGTAAAGGCGGCCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTaaa                         >  1:533475/1‑140 (MQ=255)                                                                                                                  aCCTATGTTTGGTAAAGGCGGCCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTGACCa                 >  1:108790/1‑140 (MQ=255)                                                                                                                   ccTATGTTTGGTAAAGGCGGCCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTGACCAt                <  1:146175/140‑1 (MQ=255)                                                                                                                     tATGTTTGGTAAAGGCGGCCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTGACCATCa              <  1:331614/140‑1 (MQ=255)                                                                                                                      aTGTTTGGTAAAGGCGGCCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTGACCATCaa             <  1:640716/140‑1 (MQ=255)                                                                                                                        gTTTGGTAAAGGCGGCCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTGACCATCAACg           <  2:533475/140‑1 (MQ=255)                                                                                                                        gTTTGGTAAAGGCGGCCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTGACCATCAACg           <  1:148475/140‑1 (MQ=255)                                                                                                                           tGGTAAAGGCGGCCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTGACCATCAACGGTg        <  1:475233/140‑1 (MQ=255)                                                                                                                            ggTAAAGGCGGCCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTGAc                   <  2:240687/128‑1 (MQ=255)                                                                                                                            ggTAAAGGCGGCCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTGAc                   >  1:240687/1‑128 (MQ=255)                                                                                                                            ggTAAAGGCGGCCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTGACCATCAACGGTGc       >  1:645524/1‑140 (MQ=255)                                                                                                                            ggTAAAGGCGGCCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTGACCATCAACGGTGc       <  1:12149/140‑1 (MQ=255)                                                                                                                             gTAAAGGCGGCCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTGACCATCAACGGTGca      >  1:850326/1‑140 (MQ=255)                                                                                                                                 aGGCGGCCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTGACCATCAACGGTGcacaca  >  1:579655/1‑140 (MQ=255)                                                                                                                                 aGGCGGCCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTGACCATCAACGGTGcacaca  >  2:120966/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                      ATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTGACCATCAACGGTGCACACA  >  NC_000913/493959‑494226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑