Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	665,137	A→G	C18C (TGT→TGC)	rlpA ←	rare lipoprotein RlpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	665,137	0	A	G	100.0%	167.8 / NA	48	C18C (TGT→TGC)	rlpA	rare lipoprotein RlpA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/22);  total (26/22)

GCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGC  >  NC_000913/665004‑665270                                                                                                                                      |                                                                                                                                      gcTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGcc                                                                                                                                 <  2:265537/140‑1 (MQ=255) gcTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGcc                                                                                                                                 <  2:421409/140‑1 (MQ=255)               tttGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGc                                                                                                                   >  1:85493/1‑140 (MQ=255)                  gCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCcgc                                                                                                                <  1:85840/140‑1 (MQ=255)                                  gTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGc                                                                                                >  2:279704/1‑140 (MQ=255)                                   ttCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGcc                                                                                               <  1:898040/140‑1 (MQ=255)                                   ttCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGcc                                                                                               <  2:19605/140‑1 (MQ=255)                                    tCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCa                                                                                              <  2:85493/140‑1 (MQ=255)                                     cGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCAc                                                                                             <  1:260031/140‑1 (MQ=255)                                     cGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCAc                                                                                             <  2:647788/140‑1 (MQ=255)                                            cgGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTAc                                                                                      >  1:400996/1‑140 (MQ=255)                                                       cGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCGTGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCAc                                                                           <  2:543277/140‑1 (MQ=255)                                                                 aaCTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGGGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACg                                                                 >  1:244625/1‑140 (MQ=255)                                                                         ggCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGa                                                         >  1:156944/1‑140 (MQ=255)                                                                             gTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGacaac                                                     >  1:887221/1‑140 (MQ=255)                                                                              ttACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGa                                                         <  1:71341/135‑1 (MQ=255)                                                                              ttACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGa                                                         >  2:71341/1‑135 (MQ=255)                                                                                   tACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTc                                               <  2:887221/140‑1 (MQ=255)                                                                                     ccGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCt                                             <  1:428427/140‑1 (MQ=255)                                                                                      cGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACAGGCTTTTCGACAACATTTTCCtg                                            >  1:671846/1‑140 (MQ=255)                                                                                      cGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCtg                                            <  2:319517/140‑1 (MQ=255)                                                                                      cGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCtg                                            >  1:34116/1‑140 (MQ=255)                                                                                       gCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCtgt                                           >  1:581446/1‑140 (MQ=255)                                                                                        cAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAGCATTTTCCtgtg                                          >  1:492622/1‑140 (MQ=255)                                                                                        cAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCtgtg                                          >  2:429862/1‑140 (MQ=255)                                                                                        cAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCtgtg                                          >  1:195833/1‑140 (MQ=255)                                                                                        cAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCtgtg                                          >  1:48006/1‑140 (MQ=255)                                                                                        cAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCtgt                                           >  1:756245/1‑139 (MQ=255)                                                                                        cAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCtgt                                           <  2:756245/139‑1 (MQ=255)                                                                                        cAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCt                                             >  1:786088/1‑137 (MQ=255)                                                                                        cAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCt                                             <  2:786088/137‑1 (MQ=255)                                                                                          ggCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTggg                                        <  2:400996/140‑1 (MQ=255)                                                                                           gCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGgt                                       >  1:664470/1‑140 (MQ=255)                                                                                            cTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGgtg                                      >  1:791550/1‑140 (MQ=255)                                                                                            cTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGgtg                                      >  1:713197/1‑140 (MQ=255)                                                                                                  tACACTTACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGAtt                                <  2:156944/140‑1 (MQ=255)                                                                                                       ttACCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACAt                           >  2:908570/1‑140 (MQ=255)                                                                                                         aCCGTCTGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTa                         >  1:389276/1‑140 (MQ=255)                                                                                                               tGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGATCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATcc                   >  2:602231/1‑140 (MQ=255)                                                                                                               tGTTGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATcc                   >  2:786336/1‑140 (MQ=255)                                                                                                                 ttGCTGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCg                 <  2:581446/140‑1 (MQ=255)                                                                                                                     tGACCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAAcc             >  2:873332/1‑140 (MQ=255)                                                                                                                       aCCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCa           <  1:602231/140‑1 (MQ=255)                                                                                                                       aCCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCa           >  1:320909/1‑140 (MQ=255)                                                                                                                       aCCATCATCGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCa           <  1:279704/140‑1 (MQ=255)                                                                                                                           tcatcGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTa                         >  1:334292/1‑122 (MQ=255)                                                                                                                           tcatcGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTa                         <  2:334292/122‑1 (MQ=255)                                                                                                                           tcatcGCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCAGCCa       <  1:429862/140‑1 (MQ=255)                                                                                                                                gCTTGTGCATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGc  <  2:195833/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                      GCTGGTAATCCTGATTTGCCGTCGCGTTCAGTGGTTCGAAACGCGGGTCCGCCCCGCTAATTTCAACTATAGGGCCGTTACATACCGCAGGCTGCGGTACACTTACCGTCTGTTGCTGACCATCATCGCTTGTACATGCCGCGAGCATTCCTGCCGCGATGCAGATCCCGAGCCACTGCTTACGCATTGCGCACCTCTTACACGCTTTTCGACAACATTTTCCTGTGGGTGTGGATTGACATTACAATCCCGAACCCAGCCATCAGC  >  NC_000913/665004‑665270

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑