Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	674,949	C→T	intergenic (‑166/‑69)	leuS ← / → ybeL	leucine‑‑tRNA ligase/DUF1451 domain‑containing protein YbeL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	674,949	0	C	T	100.0%	154.1 / NA	46	intergenic (‑166/‑69)	leuS/ybeL	leucine‑‑tRNA ligase/DUF1451 domain‑containing protein YbeL
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/25);  total (21/25)

CTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTCAGTTAACGACCCGGGAGATGAAACGATGAACAAGGTTGCTCAATATTACCGTGAACTGGTTGCGTCACTGAGCGAACGCCTGCGCAATGGCG  >  NC_000913/674813‑675084                                                                                                                                         |                                                                                                                                        ccaCAAATGTAGCGTTGAAGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGTGTa                                                                                                                                      >  2:611659/3‑140 (MQ=255) cTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGTGTa                                                                                                                                      >  2:154199/1‑140 (MQ=255) cTACAAATGTAGCGTCGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGTGTa                                                                                                                                      >  2:141045/1‑140 (MQ=255)  tACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGTGTAt                                                                                                                                     <  2:609613/140‑1 (MQ=255)       aTGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGTGTATTagca                                                                                                                                <  2:468493/140‑1 (MQ=255)          tAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGTGTATTagcaaag                                                                                                                             >  2:528594/1‑140 (MQ=255)           aGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGTGTATTagcaaagc                                                                                                                            >  2:748186/1‑140 (MQ=255)            gCGTCGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGTGTATTagcaaagca                                                                                                                           <  1:141045/140‑1 (MQ=255)             cGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGTGTATTagcaaagcaa                                                                                                                          >  2:208336/1‑140 (MQ=255)               ttGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGTGTATTAGCAAAGCAAgg                                                                                                                        >  1:6216/1‑140 (MQ=255)                 gAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGTGTATTAGCAAAGCAAGGaa                                                                                                                      >  2:397895/1‑140 (MQ=255)                  aGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGTGTATTAGCAAAGCAAGGAAc                                                                                                                     >  2:284681/1‑140 (MQ=255)                   ggtggtTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGTGTATTAGCAAAGCAAGGAACa                                                                                                                    >  2:665441/1‑140 (MQ=255)                    gtggtTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGTGTATTAGCAAAGCAAGGAACaa                                                                                                                   >  1:677461/1‑140 (MQ=255)                         ttttCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGTGTATTAGCAAAGCAAGGAACAAAGAAc                                                                                                              <  1:237993/140‑1 (MQ=255)                            tCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAATTTACGGAGTTTGTGGGTGTATTAGCAAAGCAAGGAACAAAGAACGTc                                                                                                           <  2:767421/140‑1 (MQ=255)                            tCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGTGTATTAGCAAAGCAAGGAACAAAGAACGTc                                                                                                           <  2:816221/140‑1 (MQ=255)                             cAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGTGTATTAGCAAAGCAAGGAACAAAGAACGTCt                                                                                                          <  1:313386/140‑1 (MQ=255)                               gATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGTGTATTAGCAAAGCAAGGAACAAAg                                                                                                                 >  2:58407/1‑131 (MQ=255)                               gATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGTGTATTAGCAAAGCAAGGAACAAAg                                                                                                                 <  1:58407/131‑1 (MQ=255)                                     catagcatagCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGTGTATTAGCAAAGCAAGGAACAAAGAACGTc                                                                                                           <  2:567811/131‑1 (MQ=255)                                     catagcatagCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGTGTATTAGCAAAGCAAGGAACAAAGAACGTc                                                                                                           >  1:567811/1‑131 (MQ=255)                                        agcatagcCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGTGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGt                                                                                               <  1:500486/140‑1 (MQ=255)                                         gcatagcCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGTGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATACTc                                                                                              <  1:611659/140‑1 (MQ=255)                                               cccAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGTGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTCAGTTaa                                                                                        >  2:893937/1‑140 (MQ=255)                                               cccAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGTGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTCAGTTaa                                                                                        <  1:154199/140‑1 (MQ=255)                                               cccAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGTGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTCAGTTaa                                                                                        >  1:756314/1‑140 (MQ=255)                                                ccAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGTGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTCAGTTAAc                                                                                       >  1:370388/1‑140 (MQ=255)                                                         cgcgTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGTGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTCAGTTAACGACCCGGga                                                                              >  1:454907/1‑140 (MQ=255)                                                                  aCAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGTGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTCAGTTAACGACCCGGGAGATGAAACg                                                                     <  1:933965/140‑1 (MQ=255)                                                                        tttCGCGCACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGTGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTCAGTTAACGACCCGGTAGATGAAACGATGaa                                                                >  1:49032/1‑139 (MQ=255)                                                                        tttCGCGCACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGTGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTCAGTTAACGACCCGGTAGATGAAACGATGaa                                                                <  2:49032/139‑1 (MQ=255)                                                                            gcgcACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGTGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTCAGTTAACGACCCGGGAGATGAAACGATGAACAAgg                                                           <  2:454907/140‑1 (MQ=255)                                                                            gcgcACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGTGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTCAGTTAACGACCCGGGAGATGAAACGATGAACAAgg                                                           <  1:893937/140‑1 (MQ=255)                                                                               cACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGTGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTCAGTTAACGACCCGGGAGATGAAACGATGAACAAGGTTg                                                        <  2:370388/140‑1 (MQ=255)                                                                                 cTCGACGTTGAAATGATGCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGTGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTCAGTTAACGACCCGGGAGATGAAACGATGAACAAGGTTGCt                                                      <  2:677461/140‑1 (MQ=255)                                                                                 cTCGACGTTGAAATGATGCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGTGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTCAGTTAACGACCCGGGAGATGAAACGATGAACAAGGTTGCt                                                      <  2:318128/140‑1 (MQ=255)                                                                                                atgCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGTGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTCAGTTAACGACCCGGGAGATGAAACGATGAACAAGGTTGCTCAATATTACCGTGaa                                       >  1:178206/1‑140 (MQ=255)                                                                                                  gCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGTGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTCAGTTAACGACCCGGGAGATGAAACGATGAACAAGGTTGCTCAATATTACCGTGAACt                                     <  1:208336/140‑1 (MQ=255)                                                                                                                  cATTAATTTACAGAGTTTGTGGGTGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTCAGTTAACGACCCGGGAGATGAAACGATGAACAAGGTTGCTCAATATTACCGTGAACTGGTTGCGTCa                           >  2:370777/1‑134 (MQ=255)                                                                                                                  cATTAATTTACAGAGTTTGTGGGTGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTCAGTTAACGACCCGGGAGATGAAACGATGAACAAGGTTGCTCAATATTACCGTGAACTGGTTGCGTCa                           <  1:370777/134‑1 (MQ=255)                                                                                                                   aTTAATTTACAGAGTTTGTGGGTGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTCAGTTAACGACCCGGGAGATGAAACGATGAACAAGGTTGCTCAATATTACCGTGAACTGGTTGCGTCACTGAGCg                    <  1:397895/140‑1 (MQ=255)                                                                                                                        tttACAGAGTTTGTGGGTGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTCAGTTAACGACCCGGGAGATGAAACGATGAACAAGGTTGCTCAATATTACCGTGAACTGGTTGCGTCACTGAGCGAACGc               <  2:756314/140‑1 (MQ=255)                                                                                                                           aCAGAGTTTGTGGGTGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTCAGTTAACGACCCGGGAGATGAAACGATGAACAAGGTTGCTCAATATTACCGTGAACTGGTTGCGTCACTGAGCGAACGCCTg            >  1:425671/1‑140 (MQ=255)                                                                                                                             agagTTTGTGGGTGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTCAGTTAACGACCCGGGAGATGAAACGATGAACAAGGTTGCTCAATATTACCGTGAACTGGTTGCGTCACt                         >  1:583511/1‑125 (MQ=255)                                                                                                                             agagTTTGTGGGTGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTCAGTTAACGACCCGGGAGATGAAACGATGAACAAGGTTGCTCAATATTACCGTGAACTGGTTGCGTCACt                         <  2:583511/125‑1 (MQ=255)                                                                                                                                 tttGTGGGTGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTCAGTTAACGACCCGGGAGATGAAACGATGAACAAGGTTGCTCAATATTACCGTGAACTGGTTGCGTCACTGAGCGAACGCCTGCGCAAt      >  2:424269/1‑140 (MQ=255)                                                                                                                                     tgGGTGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTCAGTTAACGACCCGGGAGATGAAACGATGAACAAGGTTGCTCAATATTACCGTGAACTGGTTGCGTCACTGAGCGAACGCCTGCGCAATGGCg  <  1:665441/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        CTACAAATGTAGCGTTGAGGTGGTTTTTCAGATCCGCATAGCATAGCCCAAACGTCCGCGTCAAAACAGCCTTTCGCGCACTCGACGTTGAAATGATGCCCGGATTATTCATACATTAATTTACAGAGTTTGTGGGCGTATTAGCAAAGCAAGGAACAAAGAACGTCTATTATTATAGTCAGTTAACGACCCGGGAGATGAAACGATGAACAAGGTTGCTCAATATTACCGTGAACTGGTTGCGTCACTGAGCGAACGCCTGCGCAATGGCG  >  NC_000913/674813‑675084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑