Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	683,784	T→C	N210S (AAC→AGC)	rihA ←	pyrimidine‑specific ribonucleoside hydrolase RihA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	683,784	0	T	C	100.0%	224.3 / NA	62	N210S (AAC→AGC)	rihA	pyrimidine‑specific ribonucleoside hydrolase RihA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/32);  total (30/32)

AATAACTCCGGTTTCAACAGCCAGGCGATGGTGCATGGGTCATGCAGTGGTGCGCCGACAAAGCCCCATTTTTCGTCTTTATGATATTCGAGGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGTTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGATCCCTGACTGGAAGACAATTTCTGCCGCTTCCGGGTCAACGTA  >  NC_000913/683652‑683911                                                                                                                                     |                                                                                                                                aaTAACTCCGGTTTCAACAGCCAGGCGATGGTGCATGGGTCATGCAGTGGTGCGCCGACAAAGCCCCATTTTTCGTCTTTATGATATTCGAGGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGCTACCAAt                                                                                                                          >  2:903394/1‑140 (MQ=255) aaTAACTCCGGTTTCAACAGCCAGGCGATGGTGCATGGGTCATGCAGTGGTGCGCCGACAAAGCCCCATTTTTCGTCTTTATGATATTCGAGGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGCTACCAAt                                                                                                                          >  1:946948/1‑140 (MQ=255)     aCTCCGGTTTCAACAGCCAGGCGATGGTGCATGGGTCATGCAGTGGTGCGCCGACAAAGCCCCATTTTTCGTCTTTATGATATTCGAGGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGCTACCAATcgcg                                                                                                                      <  1:638606/140‑1 (MQ=255)     aCTCCGGTTTCAACAGCCAGGCGATGGTGCATGGGTCATGCAGTGGTGCGCCGACAAAGCCCCATTTTTCGTCTTTATGATATTCGAGGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGCTACCAATcgcg                                                                                                                      <  2:289356/140‑1 (MQ=255)        ccGGTTTCAACAGCCAGGCGATGGTGCATGGGTCATGCAGTGGTGCGCCGACAAAGCCCCATTTTTCGTCTTTATGATATTCGAGGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGCTACCAATCGCGCgg                                                                                                                   >  2:585072/1‑140 (MQ=255)         cGGTTTCAACAGCCAGGCGATGGTGCATGGGTCATGCAGTGGTGCGCCGACAAAGCCCCATTTTTCGTCTTTATGATATTCGAGGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGCTACCAATCGCGCGGa                                                                                                                  <  1:374409/140‑1 (MQ=255)               cAACAGCCAGGCGATGGTGCATGGGTCATGCAGTGGTGCGCCGACAAAGCCCCATTTTTCGTCTTTATGATATTCGAGGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCt                                                                                                            >  1:150949/1‑140 (MQ=255)                  cAGCCAGGCGATGGTGCATGGGTCATGCAGTGGTGCGCCGACAAAGCCCCATTTTTCGTCTTTATGATATTCGAGGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCTCgg                                                                                                         <  2:21540/140‑1 (MQ=255)                   aGCCAGGCGATGGTGCATGGGTCATGCAGTGGTGCGCCGACAAAGCCCCATTTTTCGTCTTTATGATATTCGAGGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCTCGgt                                                                                                        <  1:903394/140‑1 (MQ=255)                   aGCCAGGCGATGGTGCATGGGTCATGCAGTGGTGCGCCGACAAAGCCCCATTTTTCGTCTTTATGATATTCGAAGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCTCGgt                                                                                                        <  2:946948/140‑1 (MQ=255)                        ggCGATGGTGCATGGGTCATGCAGTGGTGCGCCGACAAAGCCCCATTTTTCGTCTTTATGATATTCGAGGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCTCGGTGTCtt                                                                                                   <  2:866430/140‑1 (MQ=255)                          cGATGGTGCATGGGTCATGCAGTGGTGCGCCGACAAAGCCCCATTTTTCGTCTTTATGATATTCGAGGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCTCGGTGTCTTCa                                                                                                 >  2:415900/1‑140 (MQ=255)                             tGGTGCATGGGTCATGCAGTGGTGCGCCGACAAAGCCCCATTTTTCGTCTTTATGATATTCGAGGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACg                                                                                              <  2:211465/140‑1 (MQ=255)                             tGGTGCATGGGTCATGCAGTGGTGCGCCGACAAAGCCCCATTTTTCGTCTTTATGATATTCGAGGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACg                                                                                              <  1:672076/140‑1 (MQ=255)                              ggTGCATGGGTCATGCAGTGGTGCGCCGACAAAGCCCCATTTTTCGTCTTTATGATATTCGAGGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGt                                                                                             >  2:27348/1‑140 (MQ=255)                                  cATGGGTCATGCAGTGGTGCGCCGACAAAGCCCCATTTTTCGTCTTTATGATATTCGAGGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCTCGGTGTCTTCa                                                                                                 <  2:117529/132‑1 (MQ=255)                                  cATGGGTCATGCAGTGGTGCGCCGACAAAGCCCCATTTTTCGTCTTTATGATATTCGAGGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCTCGGTGTCTTCa                                                                                                 >  1:117529/1‑132 (MQ=255)                                   aTGGGTCATGCAGTGGTGCGCCGACAAAGCCCCATTTTTCGTCTTTATGATATTCGAGGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGAtt                                                                                        >  1:35470/1‑140 (MQ=255)                                     gggTCATGCAGTGGTGCGCCGACAAAGCCCCATTTTTCGTCTTTATGATATTCGAGGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTtg                                                                                      <  1:79477/140‑1 (MQ=255)                                       gTCATGCAGTGGTGCGCCGACAAAGCCCCATTTTTCGTCTTTATGATATTCGAGGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGAt                                                                                         >  2:341456/1‑135 (MQ=255)                                       gTCATGCAGTGGTGCGCCGACAAAGCCCCATTTTTCGTCTTTATGATATTCGAGGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGAt                                                                                         <  1:341456/135‑1 (MQ=255)                                       gTCATGCAGTGGTGCGCCGACAAAGCCCCATTTTTCGTCTTTATGATATTCGAGGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTtgtg                                                                                    >  1:601848/1‑140 (MQ=255)                                         cATGCAGTGGTGCGCCGACAAAGCCCCATTTTTCGTCTTTATGATATTCGAGGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCt                                                                                  <  2:698143/140‑1 (MQ=255)                                          aTGCAGTGGTGCGCCGACAAAGCCCCATTTTTCGTCTTTATGATATTCGAGGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCtt                                                                                 >  1:104369/1‑140 (MQ=255)                                          aTGCAGTGGTGCGCCGACAAAGCCCCATTTTTCGTCTTTATGATATTCGAGGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCtt                                                                                 >  1:867300/1‑140 (MQ=255)                                            gCAGTGGTGCGCCGACAAAGCCCCATTTTTCGTCTTTATGATATTCGAGGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTa                                                                               <  1:354909/140‑1 (MQ=255)                                             cAGTGGTGCGCCGACAAAGCCCCATTTTTCGTCTTTATGATATTCGAGGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTAt                                                                              <  1:585072/140‑1 (MQ=255)                                             cAGTGGTGCGCCGACAAAGCCCCATTTTTCGTCTTTATGATATTCGAGGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTAt                                                                              <  2:435947/140‑1 (MQ=255)                                                tggtgCGCCGACAAAGCCCCATTTTTCGTCTTTATGATATTCGAGGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAg                                                                           >  1:841619/1‑140 (MQ=255)                                                 ggtgCGCCGACAAAGCCCTATTTTTCGTCTTTATGATATTCGAGGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGt                                                                          >  1:743089/1‑140 (MQ=255)                                                 ggtgCGCCGACAAAGCCCCATTTTTCGTCTTTATGATATTCGAGGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCttt                                                                                >  2:218816/1‑134 (MQ=255)                                                 ggtgCGCCGACAAAGCCCCATTTTTCGTCTTTATGATATTCGAGGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCttt                                                                                <  1:218816/134‑1 (MQ=255)                                                 ggtgCGCCGACAAAGCCCCATTTTTCGTCTTTATGATATTCGAGGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTAt                                                                              <  2:535165/136‑1 (MQ=255)                                                 ggtgCGCCGACAAAGCCCCATTTTTCGTCTTTATGATATTCGAGGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTAt                                                                              >  1:535165/1‑136 (MQ=255)                                                 ggtgCGCCGACAAAGCCCCATTTTTCGTCTTTATGATATTCGAGGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGt                                                                          <  2:684753/140‑1 (MQ=255)                                                 ggtgCGCCGACAAAGCCCCATTTTTCGTCTTTATGATATTCGAGGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGt                                                                          >  2:893374/1‑140 (MQ=255)                                                  gtgCGCCGACAAAGCCCCATTTTTCGTCTTTATGATATTCGAGGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTa                                                                         >  2:739865/1‑140 (MQ=255)                                                     cgcCGACAAAGCCCCATTTTTCGTCTTTATGATATTCGAGGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACa                                                                      >  1:803220/1‑140 (MQ=255)                                                       ccGACAAAGCCCCATTTTTCGTCTTTATGATATTCGAGGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCt                                                                                  <  2:924577/126‑1 (MQ=255)                                                       ccGACAAAGCCCCATTTTTCGTCTTTATGATATTCGAGGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCt                                                                                  >  1:924577/1‑126 (MQ=255)                                                                   cATTTTTCGTCTTTATGATATTCGAGGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCAt                                                        <  1:415900/140‑1 (MQ=255)                                                                                tATGATATTCGAGGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGATc                                           >  1:394881/1‑140 (MQ=255)                                                                                      atTCGAGGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGATCCCTGAc                                     <  2:867300/140‑1 (MQ=255)                                                                                       ttCGAGGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGATCCCTGACt                                    >  2:532439/1‑140 (MQ=255)                                                                                        tCGAGGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGATCCCTGACTg                                   >  2:808074/1‑140 (MQ=255)                                                                                         cGAGGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGATCCCTGACTgg                                  >  1:25676/1‑140 (MQ=255)                                                                                            ggAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCg                                                <  2:272237/123‑1 (MQ=255)                                                                                            ggAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCg                                                >  1:272237/1‑123 (MQ=255)                                                                                            ggAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGATCCCTGACTGGAAg                               >  2:378950/1‑140 (MQ=255)                                                                                               agaaATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGGGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGATCCCTGACTGGAAGACa                            >  2:563424/1‑140 (MQ=255)                                                                                                   aTCCAGCAGTTCGGCAACAATGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGATCCCTGACTGGAAGACAAttt                        <  2:731569/140‑1 (MQ=255)                                                                                                       agcagTTCGGCAACAATGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGATCCCTGACTGGAAGACAATTTCTGc                    <  2:150949/140‑1 (MQ=255)                                                                                                        gcagTTCGGCAACAATGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCGCCACCGGGATCCCTGACTGGAAGACAATTTCTGcc                   >  2:812788/1‑140 (MQ=255)                                                                                                         cagTTCGGCAACAATGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGATCCCTGACTGGAAGACAATTTCTGCCg                  >  1:850783/1‑140 (MQ=255)                                                                                                                gCAACAATGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGATCCCTGACTGGAAGACAATTTCTGCCGCTTCCgg           <  2:803220/140‑1 (MQ=255)                                                                                                                  aacaaTGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCTGACCGGCCATCACCACCGGGATCCCTGACTGGAAGACAATTTCTGCCGCTTCCGGGt         <  2:743089/140‑1 (MQ=255)                                                                                                                  aacaaTGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGATCCCTGACTGGAAGACAATTTCTGCCGCTTCCGGGt         <  1:27348/140‑1 (MQ=255)                                                                                                                  aacaaTGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGATCCCTGACTGGAAGACAATTTCTGCCGCTTCCGGGt         <  1:808074/140‑1 (MQ=255)                                                                                                                  aacaaTGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGATCCCTGACTGGAAGACAATTTCTGCCGCTTCCGGGt         <  1:532439/140‑1 (MQ=255)                                                                                                                       tGGTTGAAACAGGGCTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGATCCCTGACTGGAAGACAATTTCTGCCGCTTCCGGGTCAACg    <  2:35470/140‑1 (MQ=255)                                                                                                                               aCAGGGCTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGATCCCTGACTGGAAGACAATTTCTGCCGCTTCCGGGTCAACGTa  >  2:781940/1‑134 (MQ=255)                                                                                                                               aCAGGGCTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGATCCCTGACTGGAAGACAATTTCTGCCGCTTCCGGGTCAACGTa  <  1:781940/134‑1 (MQ=255)                                                                                                                                     |                                                                                                                                AATAACTCCGGTTTCAACAGCCAGGCGATGGTGCATGGGTCATGCAGTGGTGCGCCGACAAAGCCCCATTTTTCGTCTTTATGATATTCGAGGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGTTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGATCCCTGACTGGAAGACAATTTCTGCCGCTTCCGGGTCAACGTA  >  NC_000913/683652‑683911

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑