Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	156,729	C→T	G52D (GGT→GAT)	yadN ←	putative fimbrial protein YadN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	156,729	0	C	T	100.0%	209.7 / NA	60	G52D (GGT→GAT)	yadN	putative fimbrial protein YadN
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/36);  total (24/36)

CTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGACCGCCGTCCATATCAGCAGAAGCAGTACCTGCAACCATCGCCAGCATAAGACCA  >  NC_000913/156600‑156854                                                                                                                                  |                                                                                                                              cTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGATCATCCTTGtt                                                                                                                     >  1:273613/1‑140 (MQ=255)         aTTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGt                                                                                                             >  2:670716/1‑140 (MQ=255)         aTTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGt                                                                                                             >  1:949226/1‑140 (MQ=255)             ggATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGTCAAc                                                                                                         <  2:594112/140‑1 (MQ=255)              taTTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGTCAAcg                                                                                                        >  2:264459/2‑140 (MQ=255)              gATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGATCATCCTTGTTAccg                                                                                                                 >  2:556471/1‑131 (MQ=255)              gATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGATCATCCTTGTTAccg                                                                                                                 <  1:556471/131‑1 (MQ=255)               aTTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGTCAAcgc                                                                                                       <  2:44705/140‑1 (MQ=255)                tttgctttgcTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGTCAAcgcg                                                                                                      <  1:10037/140‑1 (MQ=255)                tttgctttgcTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGTCAAcgcg                                                                                                      <  2:949226/140‑1 (MQ=255)                tttgctttgcTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGTCAAcgcg                                                                                                      <  2:672823/140‑1 (MQ=255)                tttgctttgcTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGTCAAcgcg                                                                                                      <  1:670716/140‑1 (MQ=255)                tttgctttgcTGCAGTCAACAGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGTCAAcgcg                                                                                                      <  2:515649/140‑1 (MQ=255)                  tgctttgctGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGTCAACGCggg                                                                                                    <  1:185600/140‑1 (MQ=255)                       tgctgcAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGTCAACGCGGGTTTca                                                                                               <  1:103733/140‑1 (MQ=255)                          tgcAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAg                                                                                            <  1:481748/140‑1 (MQ=255)                          tgcAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAg                                                                                            <  1:899333/140‑1 (MQ=255)                              gTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCAGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGgtgt                                                                                        >  2:43936/1‑140 (MQ=255)                                 aaCGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTAt                                                                                     >  2:229131/1‑140 (MQ=255)                                 aaCGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTAt                                                                                     >  1:878938/1‑140 (MQ=255)                                   cGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGTCAACGCGGGTTTca                                                                                               >  1:163765/1‑128 (MQ=255)                                   cGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGTCAACGCGGGTTTca                                                                                               <  2:163765/128‑1 (MQ=255)                                     gTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATc                                                                                    <  1:72290/137‑1 (MQ=255)                                     gTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCAGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATcaac                                                                                 <  1:43936/140‑1 (MQ=255)                                     gTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACACCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATc                                                                                    >  2:72290/1‑137 (MQ=255)                                         tGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAAcc                                                                             >  1:37982/1‑140 (MQ=255)                                         tGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAAcc                                                                             <  2:125279/140‑1 (MQ=255)                                         tGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAAcc                                                                             >  1:837517/1‑140 (MQ=255)                                           cTGAAAGGTTTAGCTTTAGCGCCAACGGTGTAATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAg                                                                           >  2:417364/1‑140 (MQ=255)                                             gAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAGAc                                                                         <  2:37982/140‑1 (MQ=255)                                                aGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAGACCAc                                                                      <  2:837517/140‑1 (MQ=255)                                                aGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAGACCAc                                                                      <  2:273613/140‑1 (MQ=255)                                                  gTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCTGCAGGATTAGATCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTg                                                                    <  2:660843/140‑1 (MQ=255)                                                  gTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTg                                                                    <  2:404091/140‑1 (MQ=255)                                                     tagctttagcGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGata                                                                 >  2:382017/1‑140 (MQ=255)                                                            agcGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAGACCAc                                                                      >  2:374541/1‑128 (MQ=255)                                                            agcGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAGACCAc                                                                      <  1:374541/128‑1 (MQ=255)                                                            agcGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACt                                                          >  1:194302/1‑140 (MQ=255)                                                             gcgcCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAGAc                                                                         <  2:193322/124‑1 (MQ=255)                                                             gcgcCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAGAc                                                                         >  1:193322/1‑124 (MQ=255)                                                               gcCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGAc                                                       <  1:382017/140‑1 (MQ=255)                                                               gcCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGAc                                                       <  2:811628/140‑1 (MQ=255)                                                                       tgtCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGAccg                                                     >  2:604532/1‑134 (MQ=255)                                                                       tgtCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGAccg                                                     <  1:604532/134‑1 (MQ=255)                                                                         tCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGACCGCCGTCCat                                             >  2:477000/1‑140 (MQ=255)                                                                                   cGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGACCGCCGTCCATATCAGCAGaa                                   >  2:705202/1‑140 (MQ=255)                                                                                      cAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGACCGCCGTCCATATcagcag                                     >  2:918949/1‑135 (MQ=255)                                                                                      cAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGACCGCCGTCCATATcagcag                                     <  1:918949/135‑1 (MQ=255)                                                                                      cAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGACCGCCGTCCATATCAGCAGAAGCa                                >  2:700757/1‑140 (MQ=255)                                                                                       aGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGACCGCCGTCCATATCAGCAGAAGCAg                               <  1:417364/140‑1 (MQ=255)                                                                                                  cACCAACGGTTGCGGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGACCGCCGTCCATATCAGCAGAAGCAGTACCTGCAAcc                    <  1:705202/140‑1 (MQ=255)                                                                                                       aCGGTTGCGGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGACCGCCGTCCATATCAGCAGAAGCAGTACCTGCAACCATCGc               <  2:878938/140‑1 (MQ=255)                                                                                                        cGGTTGCGGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGACCGCCGTCCATATCAGCAGAAGCAGTACCTGCAACCATCGcc              <  1:542364/140‑1 (MQ=255)                                                                                                        cGGTTGCGGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGACCGCCGTCCATATCAGCAGAAGCAGTACCTGCAACCATCGcc              <  1:229131/140‑1 (MQ=255)                                                                                                        cGGTTGCGGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGACCGCCGTCCATATCAGCAGAAGCAGTACCTGCAACCATCGcc              <  1:28951/140‑1 (MQ=255)                                                                                                        cGGTTGCGGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGACCGCCGTCCATATCAGCAGAAGCAGTACCTGCAACCATCGcc              <  1:669984/140‑1 (MQ=255)                                                                                                            tGCGGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGACCGCCGTCCATATCAGCAGAAGCAGTACCTGCAACCATCGCCAGCa          <  1:58703/140‑1 (MQ=255)                                                                                                            tGCGGTTTGCAGCAGGATCAGATCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGACCGCCGTCCATATCAGCAGAAGCAGTACCTGCAACCATCGCCAGCa          <  1:112388/140‑1 (MQ=255)                                                                                                                   tGCAGCAGGATCAGATCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGACCGCCGTCCATATCAGCAGAAGCAGTACCTGCAACCATCGCCAGCATAAGAcc   >  2:720865/1‑140 (MQ=255)                                                                                                                    gcagcaGGATCAGATCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGACCGCCGTCCATATCAGCAGAAGCAGTACCTGCAACCATCGCCAGCATAAGACCa  >  1:79802/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                              CTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAACGGTGTCATTCAGTACGCCAGCATCGATTTCACCAACGGTTGCGGTTTGCAGCAGGATCAGACCATCCTTGTTACCGCCGTCAACGCGGGTTTCACAGGTGTTATCAACAACCAGACCACTGATATTTAACTGACCGCCGTCCATATCAGCAGAAGCAGTACCTGCAACCATCGCCAGCATAAGACCA  >  NC_000913/156600‑156854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑