Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	894,177	C→T	P132S (CCC→TCC)	potF →	putrescine ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	894,177	0	C	T	100.0%	74.7 / NA	22	P132S (CCC→TCC)	potF	putrescine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/8);  total (14/8)

GCTTTCTGGAGCGCCAGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGATCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAACGCGCCCGTCGATAGCTGGGACTTGATCCTCAAACCTGAAAATCTGGAAAAACTG  >  NC_000913/894043‑894299                                                                                                                                       |                                                                                                                           gCTTTCTGGAGCGCCAGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGATCCCGACAATAAATTTGCTATGTCCtat                                                                                                                       >  1:37482/1‑140 (MQ=255)               cAGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGATCCCGACAATAAATTTGCTATGTCCTATATGTGGGCgacgac                                                                                                         >  1:364694/1‑140 (MQ=255)               cAGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGATCCCGACAATAAATTTGCTATGTCCTATATGTGGGCgacgac                                                                                                         <  1:594924/140‑1 (MQ=255)                    gACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGATCCCGACAATAAATTTGCTATGTCCTATATGTGGGCGACGACCGGGa                                                                                                    <  2:364694/140‑1 (MQ=255)                           ggAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGATCCCGACAATAAATTTGCTATGTCCTATATGTGGGCGACGACCGGGATTGGCta                                                                                             >  1:522357/1‑140 (MQ=255)                                      gCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGATCCCGACAATAAATTTGCTATGTCCTATATGTGGGCGACGACCGGGa                                                                                                    <  2:471491/122‑1 (MQ=255)                                      gCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGATCCCGACAATAAATTTGCTATGTCCTATATGTGGGCGACGACCGGGa                                                                                                    >  1:471491/1‑122 (MQ=255)                                         gCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGATCCCGACAATAAATTTGCTATGTCCTATATGTGGGCGACGACCGGGATTGGCt                                                                                              <  1:154457/125‑1 (MQ=255)                                         gCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGATCCCGACAATAAATTTGCTATGTCCTATATGTGGGCGACGACCGGGATTGGCt                                                                                              >  2:154457/1‑125 (MQ=255)                                                 aaaGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGATCCCGACAATAAATTTGCTATGTCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCg                                                                       >  1:235201/1‑140 (MQ=255)                                                            ccGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGATCCCGACAATAAATTTGCTATGTCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGa                                                            >  2:116283/1‑140 (MQ=255)                                                                 gTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGATCCCGACAATAAATTTGCTATGTCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAAcg                                                       <  2:235201/140‑1 (MQ=255)                                                                      agaaTCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGATCCCGACAATAAATTTGCTATGTCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAACGCGccc                                                  >  2:260169/1‑140 (MQ=255)                                                                          tctcGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGATCCCGACAATAAATTTGCTATGTCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAACGCGCCCGTCg                                              >  1:888732/1‑140 (MQ=255)                                                                                        tgctgAAGCTGGTCGCCAAACACGATCCCGACAATAAATTTGCTATGTCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAACGCGCCCGTCGATAGCTGGGACTTg                                <  1:260169/140‑1 (MQ=255)                                                                                          ctgAAGCTGGTCGCCAAACACGATCCCGACAATAAATTTGCTATGTCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAACGCGCCCGTCGATAGCTGGGACTTGAt                              >  2:316832/1‑140 (MQ=255)                                                                                                   gTCGCCAAACACGATCCCGACAATAAATTTGCTATGTCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAACGCGCCCGTCGATAGCTGGGACTTGATCCTCAAAcc                     >  2:439446/1‑140 (MQ=255)                                                                                                    tCGCCAAACACGATCCCGACAATAAATTTGCTATGTCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAACGCGCCCGTCGATAGCTGGGACTTGATCCTCAAACCt                    >  1:472917/1‑140 (MQ=255)                                                                                                     cGCCAAACACGATCCCGACAATAAATTTGCTATGTCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAACGCGCCCGTCGATAGCTGGGACTTGATCCTCAAACCTg                   <  2:37482/140‑1 (MQ=255)                                                                                                         aaaCACGATCCCGACAATAAATTTGCTATGTCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAACGCGCCCGTCGATAGCTGGGACTTGATCCTCAAACCTGaaaa               >  1:146001/1‑140 (MQ=255)                                                                                                                             aTTTGCTATGTCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAACGCGCCCGTCGATAGCTGGGACTTGATCCTCAAACCTGAAAATCTGGAAAAACTg  >  1:416912/1‑133 (MQ=255)                                                                                                                             aTTTGCTATGTCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAACGCGCCCGTCGATAGCTGGGACTTGATCCTCAAACCTGAAAATCTGGAAAAACTg  <  2:416912/133‑1 (MQ=255)                                                                                                                                       |                                                                                                                           GCTTTCTGGAGCGCCAGTTGACTGCGGGAGTTTTCCAGCCGCTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTCGCCAAACACGATCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATTGGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAACGCGCCCGTCGATAGCTGGGACTTGATCCTCAAACCTGAAAATCTGGAAAAACTG  >  NC_000913/894043‑894299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑