Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,035,711	A→G	T216A (ACC→GCC)	hyaC →	hydrogenase 1 cytochrome b subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,035,711	0	A	G	100.0%	204.6 / NA	57	T216A (ACC→GCC)	hyaC	hydrogenase 1 cytochrome b subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/31);  total (26/31)

TCTACTGGACGGGTGGCAACTCAATGGACATTCACAGCTGGCATCGGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCACCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGG  >  NC_000913/1035574‑1035845                                                                                                                                          |                                                                                                                                       tCTACTGGACGGGTGGCAACTCAATGGACATTCACAGCTGGCATCGGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTccgcc                                                                                                                                      <  2:348533/140‑1 (MQ=255)        gACGGGTGGCAACTCAATGGACATTCACAGCTGGCATCGGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCa                                                                                                                               <  2:59400/140‑1 (MQ=255)        gACGGGTGGCAACTCAATGGACATTCACAGCTGGCATCGGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCa                                                                                                                               >  1:85440/1‑140 (MQ=255)            ggTGGCAACTCAATGGACATTCACAGCTGGCATCGGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACgg                                                                                                                           <  1:279187/140‑1 (MQ=255)            ggTGGCAACTCAATGGACATTCACAGCTGGCATCGGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACgg                                                                                                                           <  1:754602/140‑1 (MQ=255)             gTGGCAACTCAATGGACATTCACAGCTGGCATCGGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCAGCCATGGTCAACGGc                                                                                                                          >  2:46668/1‑140 (MQ=255)                gCAACTCAATGGACATTCACAGCTGGCATCGGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTAc                                                                                                                       >  2:816410/1‑140 (MQ=255)                 cAACTCAATGGACATTCACAGCTGGCATCGGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTAcc                                                                                                                      <  1:569531/140‑1 (MQ=255)                  aaCTCAATGGACATTCACAGCTGGCATCGGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCg                                                                                                                     >  1:167268/1‑140 (MQ=255)                       aaTGGACATTCACAGCTGGCATCGGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGcc                                                                                                                >  2:743172/1‑140 (MQ=255)                              aTTCACAGCTGGCATCGGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCT‑CGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAAttt                                                                                                        >  2:70910/1‑140 (MQ=255)                                tCACAGCTGGCATCGGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTGGCCACAAATTTg                                                                                                       >  2:841972/1‑140 (MQ=255)                                   caGCTGGCATCGGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCa                                                                                                    <  2:395020/140‑1 (MQ=255)                                     gCTGGCATCGGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCaaa                                                                                                  >  2:96959/1‑140 (MQ=255)                                     gCTGGCATCGGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCaaa                                                                                                  <  1:79147/140‑1 (MQ=255)                                       tGGCATCGGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTGGCCACAAATTTGGCAAAAt                                                                                                <  1:841972/140‑1 (MQ=255)                                        ggCATCGGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATa                                                                                               <  2:85440/140‑1 (MQ=255)                                          cATCGGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTgg                                                                                                      >  1:121921/1‑131 (MQ=255)                                          cATCGGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTgg                                                                                                      <  2:121921/131‑1 (MQ=255)                                           aTCGGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGt                                                                                            <  2:167268/140‑1 (MQ=255)                                                   gggATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGa                                                                                    >  1:320011/1‑140 (MQ=255)                                                     gATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGc                                                                                  <  2:19965/140‑1 (MQ=255)                                                         tgGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTc                                                                              >  2:268547/1‑140 (MQ=255)                                                         tgGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTc                                                                              >  2:347649/1‑140 (MQ=255)                                                          ggCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGtt                                                                               >  2:28842/1‑138 (MQ=255)                                                          ggCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGtt                                                                               <  1:28842/138‑1 (MQ=255)                                                          ggCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCa                                                                             >  1:149371/1‑140 (MQ=255)                                                          ggCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCa                                                                             <  1:780572/140‑1 (MQ=255)                                                          ggCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCa                                                                             <  1:347649/140‑1 (MQ=255)                                                             tGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATga                                                                          <  1:361636/140‑1 (MQ=255)                                                                ttGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATgagcg                                                                       >  1:630517/1‑140 (MQ=255)                                                                  ggCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCAGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATgagcgag                                                                     <  1:46668/140‑1 (MQ=255)                                                                  ggCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATATCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATgagcgag                                                                     <  1:41264/140‑1 (MQ=255)                                                                         ttGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAAcgcg                                                              <  1:743172/140‑1 (MQ=255)                                                                           gtgATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCgtg                                                            <  2:320011/140‑1 (MQ=255)                                                                             gATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCgtggt                                                          <  1:816410/140‑1 (MQ=255)                                                                             gATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCgtggt                                                          <  1:102473/140‑1 (MQ=255)                                                                                  gTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCa                                                     <  1:96959/140‑1 (MQ=255)                                                                                   tCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCAt                                                    >  1:598128/1‑140 (MQ=255)                                                                                      tGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATggg                                                 <  1:498487/140‑1 (MQ=255)                                                                                      tGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATggg                                                 >  1:146063/1‑140 (MQ=255)                                                                                           aCATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTgg                                            >  2:262351/1‑140 (MQ=255)                                                                                            cATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTggg                                           <  1:143748/140‑1 (MQ=255)                                                                                            cATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTggg                                           <  1:535072/140‑1 (MQ=255)                                                                                            cATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTggg                                           >  2:535072/1‑140 (MQ=255)                                                                                             aTGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCt                                              <  2:25382/136‑1 (MQ=255)                                                                                             aTGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCt                                              >  1:25382/1‑136 (MQ=255)                                                                                             aTGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGc                                          >  1:512544/1‑140 (MQ=255)                                                                                                 cgcTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAAcc                                      >  1:206273/1‑140 (MQ=255)                                                                                                 cgcTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAAcc                                      >  2:703058/1‑140 (MQ=255)                                                                                                 cgcTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAAcc                                      >  2:407398/1‑140 (MQ=255)                                                                                                 cgcTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAAcc                                      >  1:739650/1‑140 (MQ=255)                                                                                                          aaGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTggg                             <  2:72896/140‑1 (MQ=255)                                                                                                          aaGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTggg                             <  2:146063/140‑1 (MQ=255)                                                                                                           aGACATCATGTCCGACGACACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGc                            <  1:70910/140‑1 (MQ=255)                                                                                                                       cgacgaCACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCtt                <  2:130915/140‑1 (MQ=255)                                                                                                                       cgacgaCACGGTGATCTCCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCtt                >  2:925561/1‑140 (MQ=255)                                                                                                                                     tctcCGCCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTgg  <  2:667197/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       TCTACTGGACGGGTGGCAACTCAATGGACATTCACAGCTGGCATCGGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCACCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGG  >  NC_000913/1035574‑1035845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑