Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,164,808	A→G	E238E (GAA→GAG)	nagZ →	beta‑N‑acetylhexosaminidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,164,808	0	A	G	100.0%	240.6 / NA	66	E238E (GAA→GAG)	nagZ	beta‑N‑acetylhexosaminidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (39/27);  total (39/27)

TGTCGGTCTTCAGTTCCTTAATCCGCGAAAATAAACTCGACGCCATTATGCCTGCGCATGTGATCTACAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCG  >  NC_000913/1164675‑1164939                                                                                                                                      |                                                                                                                                    tGTCGGTCTTCAGTTCCTTAATCCGCGAAAATAAACTCGACGCCATTATGCCTGCGCATGTGATCTACAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAGCTGGGt                                                                                                                               >  2:183456/1‑140 (MQ=255)      gTCTTCAGTTCCTTAATCCGCGAAAATAAACTCGACGCCATTATGCCTGCGCATGTGATCTACAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCGGGAGCTGGGTTTTGa                                                                                                                          >  1:853739/1‑140 (MQ=255)       tCTTCAGTTCCTTAATCCGCGAAAATAAACTCGACGCCATTATGCCTGCGCATGTGATCTACAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAGCTGGGtttt                                                                                                                            <  2:763898/137‑1 (MQ=255)       tCTTCAGTTCCTTAATCCGCGAAAATAAACTCGACGCCATTATGCCTGCGCATGTGATCTACAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAGCTGGGtttt                                                                                                                            >  1:763898/1‑137 (MQ=255)          tCAGTTCCTTAATCCGCGAAAATAAACTCGACGCCATTATGCCTGCGCATGTGATCTACAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGc                                                                                                                      <  2:4727/140‑1 (MQ=255)             gTTCCTTAATCCGCGAAAATAAACTCGACGCCATTATGCCTGCGCATGTGATCTACAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTg                                                                                                                   >  2:479824/1‑140 (MQ=255)                   tAATCCGCGAAAATAAACTCGACGCCATTATGCCTGCGCATGTGATCTACAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTtc                                                                                                             >  1:83115/1‑140 (MQ=255)                      tCCGCGAAAATAAACTCGACGCCATTATGCCTGCGCATGTGATCTACAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTctct                                                                                                          <  2:337445/140‑1 (MQ=255)                        cgcgAAAATAAACTCGACGCCATTATGCCTGCGCATGTGATCTACAGTGATGTTGATCCGCGTCGGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGAATTGCGGCAGGAGCTGGAATTAGACGGCGTGATTTTCTCTGa                                                                                                        >  2:219590/1‑140 (MQ=255)                        cgcgAAAATAAACTCGACGCCATTATGCCTGCGCATGTGATCTACAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGa                                                                                                        <  2:83115/140‑1 (MQ=255)                          cgAAAATAAACTCGACGCCATTATGCCTGCGCATGTGATCTACAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACg                                                                                                      >  2:596530/1‑140 (MQ=255)                          cgAAAATAAACTCGACGCCATTATGCCTGCGCATGTGATCTACAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACg                                                                                                      >  2:526574/1‑140 (MQ=255)                            aaaaTAAACTCGACGCCATTATGCCTGCGCATGTGATCTACAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACGAt                                                                                                    <  1:479824/140‑1 (MQ=255)                               aTAAACTCGACGCCATTATGCCTGCGCATGTGATCTACAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCGGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTa                                                                                                 <  2:853739/140‑1 (MQ=255)                                  aaCTCGACGCCATTATGCCTGCGCATGTGACCTACAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCg                                                                                              <  2:745100/140‑1 (MQ=255)                                               tATGCCTGCGCATGTGATCTACAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCcg                                                                                 <  2:251990/140‑1 (MQ=255)                                                   ccTGCGCATGTGATCTACAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGAt                                                                             >  2:459858/1‑140 (MQ=255)                                                      gcgcATGTGATCTACAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTAt                                                                          >  2:774087/1‑140 (MQ=255)                                                        gcATGTGATCTACAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATgg                                                                        >  2:877334/1‑140 (MQ=255)                                                                      gTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGaa                                                          >  1:152066/1‑140 (MQ=255)                                                                       tGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAAc                                                         >  1:551641/1‑140 (MQ=255)                                                                       tGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAAc                                                         >  1:760187/1‑140 (MQ=255)                                                                       tGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAAc                                                         >  2:319578/1‑140 (MQ=255)                                                                         aTGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATg                                                               >  1:40485/1‑132 (MQ=255)                                                                         aTGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATg                                                               <  2:40485/132‑1 (MQ=255)                                                                         aTGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAAcgc                                                       >  2:647969/1‑140 (MQ=255)                                                                         aTGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAAcgc                                                       >  2:241512/1‑140 (MQ=255)                                                                         aTGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAAcgc                                                       >  1:460925/1‑140 (MQ=255)                                                                         aTGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAAcgc                                                       >  2:418009/1‑140 (MQ=255)                                                                         aTGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTAAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTGTCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAAcgc                                                       >  2:885906/1‑140 (MQ=255)                                                                          tGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAAcgcg                                                      >  2:256540/1‑140 (MQ=255)                                                                              gATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCa                                                  >  2:40389/1‑140 (MQ=255)                                                                               aTCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAg                                                 >  1:738518/1‑140 (MQ=255)                                                                                tCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAgg                                                <  2:152066/140‑1 (MQ=255)                                                                                 ccGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGc                                               >  2:935147/1‑140 (MQ=255)                                                                                  cgcgTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAggat                                              <  1:319578/140‑3 (MQ=255)                                                                                   gcgTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTTATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCa                                                  <  2:685519/135‑1 (MQ=255)                                                                                   gcgTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTTATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCa                                                  >  1:685519/1‑135 (MQ=255)                                                                                   gcgTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCGGGCtt                                             <  2:760187/140‑1 (MQ=255)                                                                                     gTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCa                                           <  1:877334/140‑1 (MQ=255)                                                                                      tCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCggg                                                    >  2:718313/1‑130 (MQ=255)                                                                                      tCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCggg                                                    <  1:718313/130‑1 (MQ=255)                                                                                        cGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTg                                        >  2:665491/1‑140 (MQ=255)                                                                                           cGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGAt                                     <  2:785110/140‑1 (MQ=255)                                                                                             aGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGc                                   <  1:183456/140‑1 (MQ=255)                                                                                                   tctcCCTACTGGCTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTg                             <  1:40389/140‑1 (MQ=255)                                                                                                        cTACTGGCTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGata                        <  1:596530/140‑1 (MQ=255)                                                                                                        cTACTGGCTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGata                        <  1:459858/140‑1 (MQ=255)                                                                                                            tGGCTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCgg                                 >  1:210413/1‑127 (MQ=255)                                                                                                            tGGCTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCgg                                 <  2:210413/127‑1 (MQ=255)                                                                                                            tGGCTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTg                             <  1:203530/131‑1 (MQ=255)                                                                                                            tGGCTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGAt                    >  2:826687/1‑140 (MQ=255)                                                                                                            tGGCTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGCGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTg                             >  2:203530/1‑131 (MQ=255)                                                                                                             ggCTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATc                   <  1:935147/140‑1 (MQ=255)                                                                                                              gCTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGata                        <  1:683934/134‑1 (MQ=255)                                                                                                              gCTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGata                        >  2:683934/1‑134 (MQ=255)                                                                                                              gCTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATcc                  >  2:14149/1‑140 (MQ=255)                                                                                                              gCTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATcc                  >  1:714479/1‑140 (MQ=255)                                                                                                              gCTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATcc                  >  1:666168/1‑140 (MQ=255)                                                                                                              gCTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATcc                  >  2:252978/1‑140 (MQ=255)                                                                                                               cTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGt                               <  1:465643/126‑1 (MQ=255)                                                                                                               cTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGt                               >  2:465643/1‑126 (MQ=255)                                                                                                               cTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATc                   <  2:371398/138‑1 (MQ=255)                                                                                                               cTGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATc                   >  1:371398/1‑138 (MQ=255)                                                                                                                tGAAAACCGTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTg                >  2:538828/1‑140 (MQ=255)                                                                                                                        gTTTTGCGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCaa        <  2:738518/140‑1 (MQ=255)                                                                                                                              cGTCAGGAGCTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTAACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCg  >  1:322669/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                    TGTCGGTCTTCAGTTCCTTAATCCGCGAAAATAAACTCGACGCCATTATGCCTGCGCATGTGATCTACAGTGATGTTGATCCGCGTCCGGCGAGCGGTTCTCCCTACTGGCTGAAAACCGTTTTGCGTCAGGAACTGGGTTTTGACGGCGTGATTTTCTCTGACGATTTATCGATGGAAGGTGCCGCGATTATGGGCAGTTATGCCGAACGCGGGCAGGCTTCACTGGATGCGGGTTGCGATATGATCCTGGTCTGCAATAATCG  >  NC_000913/1164675‑1164939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑