Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,270,999	A→G	V284A (GTT→GCT)	chaA ←	Na(+)/K(+):H(+) antiporter ChaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,270,999	0	A	G	100.0%	148.9 / NA	41	V284A (GTT→GCT)	chaA	Na(+)/K(+):H(+) antiporter ChaA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/23);  total (18/23)

CATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAACCTGGTTGTTCAACACTGCTTTTAATGCACCTAAACCTTCCGGCGACAGAATCAACAGTGCCACCAGGAAGCCAGTAAAGGCGACAGGGGCATTCATGCTGTCGAGCAATGTCTCCAGCGAGCTGGCGTTCATTT  >  NC_000913/1270863‑1271134                                                                                                                                         |                                                                                                                                        caTCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAGCCt                                                                                                                                      <  1:933908/140‑1 (MQ=255)   tCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAGCCTgg                                                                                                                                    <  2:17934/140‑1 (MQ=255)   tCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAGCCTgg                                                                                                                                    <  2:446540/140‑1 (MQ=255)   tCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAGCCTgg                                                                                                                                    <  2:101320/140‑1 (MQ=255)      ccaccaTTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAGCCTGgttg                                                                                                                                 >  1:176785/1‑140 (MQ=255)              tCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAGCCTGGTTGTTCAacac                                                                                                                         <  1:31218/140‑1 (MQ=255)               cTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAGCCTGGTTGTTCAACACt                                                                                                                        <  1:771825/140‑1 (MQ=255)                 ggCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAGCCTGGTTGTTCAACACTGc                                                                                                                      >  2:122244/1‑140 (MQ=255)                        cAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAGCCTGGTTGTTCAACACTGCTTTTAAt                                                                                                               <  2:301485/140‑1 (MQ=255)                        cAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAGCCTGGTTGTTCAACACTGCTTTTAAt                                                                                                               <  1:855827/140‑1 (MQ=255)                            tGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAGCCTGGTTGTTCAACACTGCTTTTAATGCAc                                                                                                           >  1:849510/1‑140 (MQ=255)                                aaCTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAGCCTGGTTGTTCAACACTGCTTTTAATGCACCTaa                                                                                                       >  2:279331/1‑140 (MQ=255)                                           gTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAGCCTGGTTGTTCAACACTGCTTTTAATGCACCTAAACCTTCCGGCg                                                                                            <  1:279331/140‑1 (MQ=255)                                              aCCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAGCCTGGTTGTTCAACACTGCTTTTAATGCACCTAAACCTTCCGGCGACa                                                                                         <  1:250798/140‑1 (MQ=255)                                                   tCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAGCCTGGTTGTTCAACACTGCTTTTAATGCACCTAAACCTTCCGGCGACAGAATc                                                                                    >  1:126635/1‑140 (MQ=255)                                                        aaGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAGCCTGGTTGTTCAACACTGCTTTTAATGCACCTAAACCTTCCGGCGACAGAATCAACAg                                                                               <  2:530899/140‑1 (MQ=255)                                                            cAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAGCCTGGTTGTTCAACACTGCTTTTAATGCACCTAAACCTTCCGGCGACAGAATCAACAGTGcc                                                                           <  2:587222/140‑1 (MQ=255)                                                               ttAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAGCCTGGTTGTTCAACACTGCTTTTAATGCACCTAAACCTTCCGGCGACAGAATCAACAGTGccacc                                                                        >  2:803795/1‑140 (MQ=255)                                                                 aGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAGCCTGGTTGTTCAACACTGCTTTTAATGCACCTAAACCTTCCGGCGACAGAATCAACAGTGCCACCAg                                                                      <  1:745807/140‑1 (MQ=255)                                                                  gCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAGCCTGGTTGTTCAACACTGCTTTTAATGCACCTAAACCTTCCGGCGACAGAATCAACAGTGCCACCAgg                                                                     >  1:468023/1‑140 (MQ=255)                                                                     tGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAGCCTGGTTGTTCAACACTGCTTTTAATGCACCTAAACCTTCCGGCGACAGAATCAACAGTGCCACCAGGAAg                                                                  >  2:487000/1‑140 (MQ=255)                                                                     tGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAGCCTGGTTGTTCAACACTGCTTTTAATGCACCTAAACCTTCCGGCGACAGAATCAACAGTGCCACCAGGAAg                                                                  <  2:322448/140‑1 (MQ=255)                                                                        cgacAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAGCCTGGTTGTTCAACACTGCTTTTAATGCACCTAAACCTTCCGGCGACAGAATCAACAGTGCCACCAGGAAGCCa                                                               <  2:74020/140‑1 (MQ=255)                                                                           cAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAGCCTGGTTGTTCAACACTGCTTTTAATGCACCTAAACCTTCCGGCGACAGAATCAACAGTGCCACCAGGAAGCCAGTa                                                            <  1:531092/140‑1 (MQ=255)                                                                                  ggTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAGCCTGGTTGTTCAACACTGCTTTTAATGCACCTAAACCTTCCGGCGACAGAATCAACAGTGCCACCAGGAAGCCAGTAAAGGCGa                                                     <  2:126635/140‑1 (MQ=255)                                                                                      aGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAGCCTGGTTGTTCAACACTGCTTTTAATGCACCTAAACCTTCCGGCGACAGAATCAACAGTGCCACCAGGAAGCCAGTAAAGGCGACAgg                                                 <  2:252520/140‑1 (MQ=255)                                                                                                ttGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAGCCTGGTTGTTCAACACTGCTTTTAATGCACCTAAACCTTCCGGCGACAGAATCAACAGTGCCACCAGGAAGCCAGTAAAGGCGACAGGGGCATTCATg                                       <  2:468023/140‑1 (MQ=255)                                                                                                       cacTGAACCAAAGAACAGATTCATCGCGCGCTGAGCCTGGTTGTTCAACACTGCTTTTAATGCACCTAAACCTTCCGGCGACAGAATCAACAGTGCCACCAGGAAGCCAGTAAAGGCGACAGGGGCATTCATGCTGTCGa                                <  2:176785/140‑1 (MQ=255)                                                                                                        acTGAACCAAAGAACAGATTCATCGCGCGCTGAGCCTGGTTGTTCAACACTGCTTTTAATGCACCTAAACCTTCCGGCGACAGAATCAACAGTGCCACCAGGAAGCCAGTAAAGGCGACAGGGGCATTCATGCTGTCGAg                               >  2:906097/1‑140 (MQ=255)                                                                                                            aaCCAAAGAACAGATTCATCGCGCGCTGAGCCTGGTTGTTCAACACTGCTTTTAATGCACCTAAACCTTCCGGCGACAGAATCAACAGTGCCACCAGGAAGCCAGTAAAGGCGACAGGGGCATTCa                                         <  1:902912/126‑1 (MQ=255)                                                                                                            aaCCAAAGAACAGATTCATCGCGCGCTGAGCCTGGTTGTTCAACACTGCTTTTAATGCACCTAAACCTTCCGGCGACAGAATCAACAGTGCCACCAGGAAGCCAGTAAAGGCGACAGGGGCATTCa                                         >  2:902912/1‑126 (MQ=255)                                                                                                            aaCCAAAGAACAGATTCATCGCGCGCTGAGCCTGGTTGTTCAACACTGCTTTTAATGCACCTAAACCTTCCGGCGACAGAATCAACAGTGCCACCAGGAAGCCAGTAAAGGCGACAGGGGCATTCATGCTGTCGAGCAAt                           >  1:9000/1‑140 (MQ=255)                                                                                                            aaCCAAAGAACAGATTCATCGCGCGCTGAGCCTGGTTGTTCAACACTGCTTTTAATGCACCTAAACCTTCCGGCGACAGAATCAACAGTGCCACCAGGAAGCCAGTAAAGGCGACAGGGGCATTCATGCTGTCGAGCAAt                           >  1:583099/1‑140 (MQ=255)                                                                                                            aaCCAAAGAACAGATTCATCGCGCGCTGAGCCTGGTTGTTCAACACTGCTTTTAATGCACCTAAACCTTCCGGCGACAGAATCAACAGTGCCACCAGGAAGCCAGTAAAGGCGACAGGGGCATTCATGCTGTCGAGCAAt                           >  1:108737/1‑140 (MQ=255)                                                                                                             aCCAAAGAACAGATTCATCGCGCGCTGAGCCTGGTTGTTCAACACTGCTTTTAATGCACCTAAACCTTCCGGCGACAGAATCAACAGTGCCACCAGGAAGCCAGTAAAGGCGACAGGGGCATTCATGCTGTCGAGCAATg                          >  1:611050/1‑140 (MQ=255)                                                                                                              ccAAAGAACAGATTCATCGCGCGCTGAGCCTGGTTGTTCAACACTGCTTTTAATGCACCTAAACCTTCCGGCGACAGAATCAACAGTGCCACCAGGAAGCCAGTAAAGGCGACAGGGGCATTCATGCTGTCGAGCAATGt                         >  1:81172/1‑140 (MQ=255)                                                                                                                   gAACAGATTCATCGCGCGCTGAGCCTGGTTGTTCAACACTGCTTTTAATGCACCTAAACCTTCCGGCGACAGAATCAACAGTGCCACCAGGAAGCCAGTAAAGGCGACAGGGGCATTCATGCTGTCGAGCAATGTCTCCa                    <  2:807099/140‑1 (MQ=255)                                                                                                                        gATTCATCGCGCGCTGAGCCTGGTTGTTCAACACTGCTTTTAATGCACCTAAACCTTCCGGCGACAGAATCAACAGTGCCACCAGGAAGCCAGTAAAGGCGACAGGGGCATTCATGCTGTCGAGCAATGTCTCCAGCGAg               <  2:108737/140‑1 (MQ=255)                                                                                                                           tCATCGCGCGCTGAGCCTGGTTGTTCAACACTGCTTTTAATGCACCTAAACCTTCCGGCGACAGAATCAACAGTGCCACCAGGAAGCCAGTAAAGGCGACAGGGGCATTCATGCTGTCGAGCAATGTCTCCAGCGAGCTg            >  2:882907/1‑140 (MQ=255)                                                                                                                              tCGCGCGCTGAGCCTGGTTGTTCAACACTGCTTTTAATGCACCTAAACCTTCCGGCGACAGAATCAACAGTGCCACCAGGAAGCCAGTAAAGGCGACAGGGGCATTCATGCTGTCGAGCAATGTCTCCAGCGAGCTGGCg         >  1:84628/1‑140 (MQ=255)                                                                                                                                     cTGAGCCTGGTTGTTCAACACTGCTTTTAATGCACCTAAACCTTCCGGCGACAGAATCAACAGTGCCACCAGGAAGCCAGTAAAGGCGACAGGGGCATTCATGCTGTCGAGCAATGTCTCCAGCGAGCTGGCGTTCAttt  >  2:857730/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        CATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACTGAACCAAAGAACAGATTCATCGCGCGCTGAACCTGGTTGTTCAACACTGCTTTTAATGCACCTAAACCTTCCGGCGACAGAATCAACAGTGCCACCAGGAAGCCAGTAAAGGCGACAGGGGCATTCATGCTGTCGAGCAATGTCTCCAGCGAGCTGGCGTTCATTT  >  NC_000913/1270863‑1271134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑